Agilent Technologies InfinityLab LC Series User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II Fraction Collectors
Inlet / Waste Tubings Replacement
This technical note describes the installation of the inlet/waste tubing kit to a
1260 Infinity II Fraction Collector:
•Agilent InfinityLab LC Series 1260 Infinity II Preparative-Scale Fraction
Collector (G1364E)
•Agilent InfinityLab LC Series 1260 Infinity II Analytical-Scale Fraction
Collector (G1364F)
•Agilent InfinityLab LC Series 1260 Infinity II Bio-inert Fraction Collector
(G5664B)
Contents
Replace the Inlet/Waste Tubings 2
Replace the Valve to Needle Tubing 8

2
Replace the Inlet/Waste Tubings
For bio-inert modules use bio-inert parts only!
When • When contaminated, worn out or visibly damaged
•Typically once every year
Parts required p/n Description
Preparative-scale:
G1364-68605 Tubing Kit 40 – 100 mL/min, 1.2 mm ID
Standard tubing kit for Preparative Fraction Collector
G1364-68603 Tubing Kit 4 – 8 mL/min, 0.5 mm ID (OPTIONAL)
G1364-68604 Tubing Kit 15 – 40 mL/min, 0.8 mm ID (OPTIONAL)
Analytical-scale and bio-inert:
G1364-68602 Tubing Kit 1 – 5 mL/min, 0.25 mm ID
Standard tubing kit for Analytical and Bio-inert Fraction
Collector
G1364-68601 Tubing Kit 1 mL/min, 0.15 mm ID (OPTIONAL)
G1364-68603 Tubing Kit 4 – 8 mL/min, 0.5 mm ID (OPTIONAL)
Preparations • Position the transport unit of the fraction collector in the Home position.
•Remove all installed trays from the tray base.
•Position the transport unit of the fraction collector in the Change Parts
position.
•Turn OFF the instrument.
•Remove the rear end of the fraction collector’s waste tubing from the
waste container, unscrew the front end of the fraction collector’s inlet
tubing from the flow cell of the detector.
WARNING
Personal injury
Risk of personal injury caused by the needle arm movement.
➔Keep fingers away from the needle area.

3
1Locate the diverter valve with the finger tight fittings of the inner tubing kit.
2Unscrew the two finger-tight fittings of the inner tubing kit at the diverter
valve.
CAUTION
Liquid spills or fraction losses
Worn or damaged tubings can cause potential spills or lead to
fraction losses.
➔Explicitly follow the described installation procedures to
maximize the lifetime of inlet / waste tubing assembly and the
valve to needle tubing, and to avoid potential spills or fraction
losses.
➔Regularly inspect the tubings and exchange them if they are
worn out or show visible signs of damage.
NOTE
Connect the tubings as described in order to maximize their lifetime
and operating security.

4
3Unclip the inlet / waste tubing assembly from the leak adapter.
4Remove the outer tubing kit from the unions.
5Remove the leak adapter from the module housing.
6Remove the unions from the inner tubing kit.

5
7Remove the clip by pushing it down through the module housing.
8Remove the inner tubing kit by pulling it down through the opening in the
module housing.
9Slide in the new inner tubing kit from bottom to top (1.) and let the clip click
into position (2.).
10 Install the unions to the inner tubing kit.
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6
11 Install the leak adapter to the module housing.
12 Connect the outer tubing kit to the unions.
13 Clip the inlet / waste tubing assembly into the leak adapter.
14 A color coded ring on one of the tubings and the valve body indicates, which
tubing belongs to which port.

7
15 Connect the finger-tight fittings of the inner tubing kit to the ports of the
diverter valve. The tubings must run into the ports of the diverter valve in
horizontal, parallel lines.
NOTE
The tubings must not be bent or twisted.
16 Connect the tubing with the label with the arrow (1) to the flow cell of the
detector. Insert the other tubing (2) into the waste container.
17 Re-install the tray(s) in the tray base.
18 Start the instrument.
19 Close the front cover.
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8
Replace the Valve to Needle Tubing
For bio-inert modules use bio-inert parts only!
When • When contaminated, worn out or visibly damaged
•Typically once every year
Tools required p/n Description
8710-1534 Wrench, open end, 4 mm
8710-0510 Wrench open 1/4 — 5/16 inch
Parts required p/n Description
Preparative-scale:
G1364-68605 Tubing Kit 40 – 100 mL/min, 1.2 mm ID
Analytical-scale and bio-inert:
G1364-68602 Tubing Kit 1 – 5 mL/min, 0.25 mm ID
Standard tubing kit for Analytical and Bio-inert Fraction
Collector
G1364-68601 Tubing Kit 1 mL/min, 0.15 mm ID (OPTIONAL)
Preparations • Position the transport unit of the fraction collector in the Home position.
•Remove all installed trays from the tray base.
•Position the transport unit of the fraction collector in the Change Parts
position and turn off the instrument.
•It might be more convenient to remove the needle from its carrier before
unscrewing the needle tubing.
WARNING
Personal injury
Risk of personal injury caused by the needle arm movement.
➔Keep fingers away from the needle area.

9
1Locate the diverter valve with the finger-tight fittings of the valve to needle
tubing assembly.
2Unscrew the finger-tight fitting of the valve to needle tubing assembly at the
diverter valve.
CAUTION
Liquid spills or fraction losses
Worn or damaged tubings can cause potential spills or lead to
fraction losses.
➔Explicitly follow the described installation procedures to
maximize the lifetime of inlet / waste tubing assembly and the
valve to needle tubing, and to avoid potential spills or fraction
losses.
➔Regularly inspect the tubings and exchange them if they are
worn out or show visible signs of damage.
NOTE
Install the tubing as described to maximize its lifetime.

10
3Using the 4 mm wrench and the 5/16“ wrench for counter-holding, unscrew
the valve to needle tubing from the needle.
4Un-clip the tubing from the bottom of the needle carrier assembly.
5Slide the tubing through the hole in the needle carrier assembly (from
bottom to top) (1.) and out of the holder in the z-arm assembly (2.).
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