Agilent Technologies 1290 Infinity II User manual

Agilent Technologies
Agilent 1290 Infinity II
Variable Wavelength
Detector
User Manual

Agilent 1290 Infinity II VWD User Manual
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Manual Part Number
G7114-90000 Rev. B
Edition
12/2014
Printed in Germany
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Agilent 1290 Infinity II VWD User Manual 3
In This Book
In This Book
This manual covers the Agilent 1290 Infinity II Variable Wavelength
Detector (G7114B).
Find information on other Agilent Variable Wavelength Detectors in
separate manuals.
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument overview
and internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements, physical
and performance specifications.
3 Using the Module
This chapter explains the essential operational parameters of the module.
4 Preparing the Module
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
5 Optimizing the Detector
This chapter provides information on how to optimize the detector.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of detector error messages, and
provides information on probable causes and suggested actions how to
recover from error conditions.

4 Agilent 1290 Infinity II VWD User Manual
In This Book
8TestFunctions
This chapter describes the detector’s built in test functions.
9 Maintenance
This chapter provides general information on maintenance of the detector.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1290 Infinity II VWD User Manual 5
Contents
Contents
1 Introduction to the Variable Wavelength Detector 9
Introduction to the Detector 10
Product Description 11
Optical System Overview 12
Dual-Wavelength Mode 17
Leak and Waste Handling 18
Operating Principle 21
2 Site Requirements and Specifications 23
Site Requirements 24
Physical Specifications 27
Performance Specifications G7114B 28
3 Using the Module 31
Magnets 32
Turn on/off 33
Status Indicators 34
Instrument Configuration 35
Set up the Detector with Agilent Open Lab ChemStation 37
The Detector User Interface 38
Detector Control Settings 40
Method Parameter Settings 41
Scanning with the VWD 45
4 Preparing the Module 47
Leak and Waste Handling 48
Setting up an Analysis 50
Solvent Information 57

6 Agilent 1290 Infinity II VWD User Manual
Contents
5 Optimizing the Detector 63
Introduction 64
Match the Flow Cell to the Column 65
Set the Detector Parameters 68
Warm up of the Detector 69
6 Troubleshooting and Diagnostics 71
Available Tests versus Interfaces 72
Agilent Lab Advisor Software 73
7 Error Information 75
What Are Error Messages 77
General Error Messages 78
Detector Error Messages 85
8TestFunctions 95
Introduction 96
Intensity Test 97
Cell Test 100
Wavelength Verification-Calibration 102
ASTM Drift and Noise Test 104
Quick Noise Test 105
Dark Current Test 107
Holmium Oxide Test 109
D/A Converter (DAC) Test 112
Other Lab Advisor Functions 115
9 Maintenance 117
Introduction to Maintenance 118
Warnings and Cautions 118
Overview of Maintenance 120
Cleaning the Module 121
Remove and Install the Doors 122
Replace the Deuterium Lamp 123
Replace the Flow Cell / Cuvette Holder 126
Repairing the Flow Cells 128

Agilent 1290 Infinity II VWD User Manual 7
Contents
Using the Cuvette Holder 131
Correcting Leaks 133
Replacing Leak Handling System Parts 135
Replace the Module Firmware 136
10 Parts and Materials for Maintenance 139
Overview of Maintenance Parts 140
Standard Flow Cell 10 mm / 14 µL 142
Micro Flow Cell 3 mm / 2 µL 144
Semi-micro Flow Cell 6 mm / 5 µL 146
High Pressure Flow Cell 10 mm / 14 µL 148
Cuvette Holder 150
Accessory Kit 151
11 Identifying Cables 153
Cable Overview 154
Analog Cables 156
Remote Cables 158
CAN/LAN Cables 162
RS-232 Cables 163
USB Cables 164
12 Hardware Information 165
Firmware Description 166
Electrical Connections 169
Interfaces 172
Setting the 6-bit Configuration Switch 179
Instrument Layout 183
Early Maintenance Feedback 184

8 Agilent 1290 Infinity II VWD User Manual
Contents
13 LAN Configuration 187
What You Have to Do First 188
TCP/IP parameter configuration 189
Configuration Switch 190
Initialization Mode Selection 191
Dynamic Host Configuration Protocol (DHCP) 195
Manual Configuration 198
PC and Agilent ChemStation Setup 202
14 Appendix 211
General Safety Information 212
Waste Electrical and Electronic Equipment Directive 220
Radio Interference 221
Sound Emission 222
Solvent Information 223
Declaration of Conformity for HOX2 Filter 224
Agilent Technologies on Internet 225

9
Agilent 1290 Infinity II VWD User Manual
Agilent Technologies
1
Introduction to the Variable Wavelength
Detector
Introduction to the Detector 10
Product Description 11
Optical System Overview 12
Flow Cell 13
Lamp 14
Source Lens Assembly 14
Entrance Slit Assembly 14
Filter Assembly 15
Mirror Assemblies M1 and M2 16
Grating Assembly 16
Beam Splitter Assembly 16
Photo Diodes Assemblies 16
Photo Diode ADC (analog-to-digital converter) 16
Dual-Wavelength Mode 17
Leak and Waste Handling 18
Waste Concept 20
Operating Principle 21
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1290 Infinity II VWD User Manual
1Introduction to the Variable Wavelength Detector
Introduction to the Detector
Introduction to the Detector
The Agilent variable wavelength detectors described in this manual are
designed for highest optical performance, GLP compliance and easy
maintenance with:
•higher data rate up to 240 Hz for ultra- fast- HPLC (G7114B),
•deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
•optional flow- cell cartridges (standard 10 mm, 14 µL; high pressure
10 mm, 14 µL; micro 3 mm, 2 µL; semi- micro 6 mm, 5 µL) and Prep
Cells are available and can be used depending on the application needs
(other types may be introduced later),
•Dual wavelength mode, see “Dual- Wavelength Mode” on page 17.
•easy front access to lamp and flow cell for fast replacement,
•electronic identification of flow cell and lamp with RFID (Radio
Frequency Identification) tag for unambiguous identification,
•lamp information: part number, serial number, production date,
ignitions, burn time
•cell information: part number, serial number, production date,
nominal path length, volume, maximum pressure
•built- in electronic temperature control (ETC) for improved baseline
stability, and
•built- in holmium oxide filter for fast wavelength accuracy verification.

Agilent 1290 Infinity II VWD User Manual 11
Introduction to the Variable Wavelength Detector 1
Product Description
Product Description
The Agilent 1290 Infinity II Variable Wavelength Detector (VWD) is the
most sensitive and fastest detector in its class.
Time- programmable wavelength switching provides sensitivity and
selectivity for your applications.
More sample information can be acquired in the dual wavelength mode.
Low detector noise (<±1.5 µAU) and baseline drift (< 1 x 10- 4 AU/h)
facilitates precise quantification of trace levels components.
High productivity can be achieved with fast analysis at up to 240 Hz data
rates.
Figure 1 Overview of the Variable Wavelength Detector
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12 Agilent 1290 Infinity II VWD User Manual
1Introduction to the Variable Wavelength Detector
Optical System Overview
Optical System Overview
The optical system of the detector is shown in the figure below. Its
radiation source is a deuterium- arc discharge lamp for the ultraviolet (UV)
wavelength range from 190 to 600 nm. The light beam from the deuterium
lamp passes through a lens, a filter assembly, an entrance slit, a spherical
mirror (M1), a grating, a second spherical mirror (M2), a beam splitter,
and finally through a flow cell to the sample diode. The beam through the
flow cell is absorbed depending on the solutions in the cell, in which UV
absorption takes place, and the intensity is converted to an electrical
signal by means of the sample photodiode. Part of the light is directed to
the reference photodiode by the beam splitter to obtain a reference signal
for compensation of intensity fluctuation of the light source. A slit in front
of the reference photodiode cuts out light of the sample bandwidth.
Wavelength selection is made by rotating the grating, which is driven
directly by a stepper motor. This configuration allows fast change of the
wavelength. The cutoff filter is moved into the lightpath above 370 nm to
reduce higher order light.
Figure 2 Optical Path of the Variable Wavelength Detector
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Agilent 1290 Infinity II VWD User Manual 13
Introduction to the Variable Wavelength Detector 1
Optical System Overview
Flow Cell
A variety of flow- cell cartridges can be inserted using the same quick and
simple mounting system.
The flow cells have an integrated RFID tag that contains the flow cell
specific information (e.g. part number, cell volume, path length, ...). A
RFID tag reader reads out this information and transfers it to the user
interface.
Figure 3 Flow Cell with RFID tag
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Table 1 Flow Cell Data
STD Semi-micro Micro High Pressure
Maximum pressure 40 (4) 40 (4) 120 (12) 400 (40) bar
(MPa)
Path length 10 (conical) 6 (conical) 3 (conical) 10 (conical) mm
Volume 14 5 2 14 µL
Inlet i.d. 0.25 0.17 0.12 0.25 mm
Inlet length 750 250 310 750 mm
Outlet i.d. 0.30 0.17 0.17 0.17 mm
Outlet length 120 120 120 120 mm
Total volume 60.77 14.49 14.00 60.77 µL
Materials in contact
with solvent
SST, quartz, PTFE,
PEEK
SST, quartz, PTFE SST, quartz, PTFE SST, quartz, Kapton

14 Agilent 1290 Infinity II VWD User Manual
1Introduction to the Variable Wavelength Detector
Optical System Overview
Lamp
The light source for the UV wavelength range is a deuterium lamp. As a
result of plasma discharge in a low pressure deuterium gas, the lamp
emits light over the 190 – 600 nm wavelength range.
The lamp has an integrated RFID tag that contains the lamp specific
information (e.g. part number, burn time, ...). A RFID tag reader reads out
this information and transfers it to the user interface.
Source Lens Assembly
The source lens receives the light from the deuterium lamp and focuses it
onto the entrance slit.
Entrance Slit Assembly
The entrance slit assembly has an exchangeable slit. The standard one has
a 1- mm slit. For replacement and calibration purposes to optimize the
alignment, a slit with a hole is needed.

Agilent 1290 Infinity II VWD User Manual 15
Introduction to the Variable Wavelength Detector 1
Optical System Overview
Filter Assembly
The filter assembly is electromechanically actuated. During wavelength
calibrations it moves into the light path.
Figure 4 Filter Assemby
The filter assembly has two filters installed and is processor- controlled.
A photo sensor determines the correct position.
OPEN nothing in light path at λ<370nm
CUTOFF cut off filter in light path at λ>370nm
HOLMIU
M
holmium oxide filter for wavelength check
SHUTTER for measurement of dark current of photo
diodes
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16 Agilent 1290 Infinity II VWD User Manual
1Introduction to the Variable Wavelength Detector
Optical System Overview
Mirror Assemblies M1 and M2
The instrument contains two spherical mirrors (M1 and M2). The beam
adjustable is vertically and horizontally. Both mirrors are identical.
Grating Assembly
The grating separates the light beam into all its component wavelengths
and reflects the light onto mirror #2.
The stepper motor reference position is determined by a plate fitted onto
the motor shaft, interrupting the beam of a photo sensor. The wavelength
calibration of the grating is done at the zero order light position and at
656 nm, which is the emission line of the deuterium lamp.
Beam Splitter Assembly
The beam splitter splits the light beam. One part goes directly to the
sample diode. The other part of the light beam goes to the reference
diode.
Photo Diodes Assemblies
Two photo diode assemblies are installed in the optical unit. The sample
diode assembly is located on the left side of the optical unit. The
reference diode assembly is located in the front of the optical unit.
Photo Diode ADC (analog-to-digital converter)
The photo diode current is directly converted to digital data direct photo
current digitalization. The data is transferred to the detector main board .
The photo diode ADC boards are located close to the photo diodes.

Agilent 1290 Infinity II VWD User Manual 17
Introduction to the Variable Wavelength Detector 1
Dual-Wavelength Mode
Dual-Wavelength Mode
The detector provides a Dual- Wavelength mode that offers additional
operation functions.
Features:
•200 ms acquisition time per data point
•5 Hz data rate distributed to two channels
•2.5 Hz data rate for each channel,
•delta wavelength max. 150 nm,
•scans during Dual- Wavelength mode are possible,
•the second order filter is disabled when one wavelength is < 370 nm.
Timetable:
•Wavelength settings are timetable programmable (depends if enough
time for implementation is available),
•switching from Single- Wavelength mode to Dual- Wavelength mode is
NOT timetable programmable,
•filter settings are not timetable programmable.

18 Agilent 1290 Infinity II VWD User Manual
1Introduction to the Variable Wavelength Detector
Leak and Waste Handling
Leak and Waste Handling
The 1290 Infinity II Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
Figure 5 Leak and waste handling concept (overview - typical stack configuration as an
example)

Agilent 1290 Infinity II VWD User Manual 19
Introduction to the Variable Wavelength Detector 1
Leak and Waste Handling
The solvent cabinet is designed to store a maximum volume of 6 L solvent.
The maximum volume for an individual bottle stored in the solvent cabinet
should not exceed 4 L. For details, see the usage guideline for the Agilent
1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has
been shipped with the solvent cabinet, electronic copies are available on
the Internet).
All leak plane outlets are situated in a consistent position so that all
Infinity and Infinity II modules can be stacked on top of each other. Waste
tubes are guided through a channel on the right hand side of the
instrument, keeping the front access clear from tubes.
The leak plane provides leak management by catching all internal liquid
leaks, guiding them to the leak sensor for leak detection, and passing
them on to the next module below, if the leak sensor fails. The leak sensor
in the leak plane stops the running system as soon as the leak detection
level is reached.
Solvent and condensate is guided through the waste channel into the
waste container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler (condensate)
•from the Seal Wash Sensor
•from the pump's Purge Valve or Multipurpose Valve
The waste tube connected to the leak pan outlet on each of the bottom
instruments guides the solvent to a suitable waste container.

20 Agilent 1290 Infinity II VWD User Manual
1Introduction to the Variable Wavelength Detector
Leak and Waste Handling
Waste Concept
1Agilent recommends using the 6 L waste can with 1 Stay
Safe cap GL45 with 4 ports (5043-1221) for optimal and
safe waste disposal. If you decide to use your own waste
solution, make sure that the tubes don't immerse in the
liquid.