Agilent Technologies Infinity Binary Pump VL 1290 User manual

Agilent Technologies
Agilent 1290 Infinity
Binary Pump VL
User Manual

Agilent 1290 Infinity Binary Pump User Manual
Notices
© Agilent Technologies, Inc. 2009,
2010-2012
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G4220-90005 Rev. C
Edition
08/2012
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
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operating procedure, practice, or
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stood and met.

Agilent 1290 Infinity Binary Pump User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1290 Infinity Binary Pump (G4220B).
1 Introduction
This chapter gives an introduction to the pump, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your system
and the installation of your Agilent 1290 Infinity Binary Pump.
4UsingthePump
This chapter explains the operational parameters of the Agilent 1290 Infinity
Binary Pump.
5 How to Optimize the Performance of Your Module
This chapter gives hints on how to optimize the performance or use additional
devices.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent 1290 Infinity Binary Pump User Manual
In This Guide…
8 Test Functions and Calibrations
This chapter describes the tests for the module.
9 Maintenance
This chapter describes the maintenance of the Agilent 1290 Infinity Binary
Pump.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter summarizes information on all cables.
12 Hardware Information
This chapter describes the pump in more detail on hardware and electronics.
13 LAN Configuration
This chapter provides information on connecting the detector to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1290 Infinity Binary Pump User Manual 5
Contents
Contents
1 Introduction 9
Features 10
Overview of the Binary Pump 11
Pump Principle 12
2 Site Requirements and Specifications 15
Site Requirements 16
Physical Specifications 19
Specifications 20
3 Installing the Module 23
Unpacking the Module 24
Optimizing the Stack Configuration 26
Removing the Transport Foam 31
Installing the Pump 32
Flow connections to the pump 35
Installation of seal wash option 39
4UsingthePump 41
Preparing the Binary Pump 42
Algae Growth in HPLC Systems 43
Setting up the Pump with the Instrument Control Interface 44
Priming the Pump 54
Solvent Information 56
5 How to Optimize the Performance of Your Module 61
Delay Volume and Extra-Column Volume 62
How to Configure the Optimum Delay Volume 63
How to Achieve Higher Resolution 65
Using Solvent Calibration Tables 68

6 Agilent 1290 Infinity Binary Pump User Manual
Contents
6 Troubleshooting and Diagnostics 69
Overview of the Module’s Indicators and Test Functions 70
Status indicators 71
Available Tests vs User Interfaces 73
Agilent Lab Advisor Software 74
7 Error Information 75
What Are Error Messages 77
General Error Messages 78
Pump Error Messages 85
8 Test Functions and Calibrations 101
Introduction 102
System Pressure Test 103
Pump Head Leak Test 105
9 Maintenance 109
Introduction to Maintenance 110
Warnings and Cautions 112
Overview of Maintenance 113
Cleaning the Module 114
Installing Fittings and Capillaries 115
Replacing the Shutoff Valve Panel 116
Replacing the Pressure Sensor 118
Replacing the Inlet valve 120
Replacing the Outlet valve 122
Replacing the Solvent Selection Valve (SSV) 125
Changing configuration or replacing the Jet Weaver 127
Replacing the Seal Wash Pump 130
Releasing a Stuck Inlet Valve 131
Replacing the Pump Heads 134
Disassembling the Pump Head 139
Disassembling the Primary Pump Head 140
Disassembling the Secondary Pump Head 143
Assembling the Pump Head 146
Replacing the Purge Valve Head 154
Replacing Parts of the High Pressure Filter Assembly 157

Agilent 1290 Infinity Binary Pump User Manual 7
Contents
Installing the Valve Rail Kit 159
Replacing the Main Power Fuses 160
Replacing Module Firmware 162
Preparing the Pump Module for Transport 163
10 Parts and Materials for Maintenance 167
Overview of Maintenance Parts 168
Capillaries 169
Solvent Cabinet Kit 170
Seal Wash Option 171
Pump Head Assembly Parts 172
Primary Pump Head Parts 174
Secondary Pump Head Parts 178
Purge Valve 182
Cover Parts 183
Leak Parts 184
Fuses 184
Accessory Kit 185
Others 186
11 Identifying Cables 189
Cable Overview 190
Analog cables 192
Remote Cables 194
BCD Cables 197
CAN/LAN Cable 199
RS-232 Cable Kit 200
Agilent 1200 Module to Printer 201
12 Hardware Information 203
Firmware Description 204
Electrical Connections 207
Interfaces 209
Setting the 8-bit Configuration Switch 216
Instrument Layout 220
Early Maintenance Feedback 221

8 Agilent 1290 Infinity Binary Pump User Manual
Contents
13 LAN Configuration 223
What You Have to Do First 224
TCP/IP parameter configuration 226
Configuration Switch 227
Initialization mode selection 228
Dynamic Host Configuration Protocol (DHCP) 232
Link configuration selection 236
Automatic configuration with Bootp 237
Manual Configuration 247
PC and User Interface Software Setup Setup 253
14 Appendix 255
General Safety Information 256
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002-96-EC) 259
Radio Interference 260
Sound Emission 261
Agilent Technologies on Internet 262

9
Agilent 1290 Infinity Binary Pump User Manual
Agilent Technologies
1
Introduction
Features 10
Overview of the Binary Pump 11
Pump Principle 12
This chapter gives an introduction to the pump, instrument overview and
internal connectors.

10 Agilent 1290 Infinity Binary Pump User Manual
1Introduction
Features
Features
The Binary pump is designed for highest performance, GLP compliance and
easy maintenance. It includes the following features:
•Seal wash for continued high lifetime of pump seals using high buffer
concentrations.
•Solvent selection valve for method flexibility.
•Jet Weaver for optimum mixing performance with a minimum of delay
volume.
•Automatic purge function for ease of use and unattended preparation of the
system.
•Auto tuning of the delivery cycle for compensation of elasticity and dead
volume effects.
•Solvent selection for optimum density correction.
•Fast defill function to increase robustness of the pump.
•Two pistons in series design for increased reliability.
•Smooth motion control to prevent shock movements.
For specifications, see Table 2 on page 20.
NOTE
This Binary pump has been introduced together with the Agilent 1290 Infinity Liquid
Chromatograph.

Agilent 1290 Infinity Binary Pump User Manual 11
Introduction 1
Overview of the Binary Pump
Overview of the Binary Pump
The Agilent 1290 Infinity Binary Pump comprises two identical pumps
integrated into one housing. Binary gradients are created by high-pressure
mixing. A degassing unit is included for applications that require best flow
stability, especially at low flow rates, for maximum detector sensitivity. The
flow path of the pump has been optimized for minimal delay of gradients.
Typical applications are high throughput methods with fast gradients on high
resolution 2.1 mm columns. The pump is capable of delivering flow in the
range of 0.05 -2 mL/min against up to 1200 bar. A solvent selection valve
allows forming binary mixtures (isocratic or gradient) from one of two
solvents per channel. Active seal wash (optional) is available for use with
concentrated buffer solutions.

12 Agilent 1290 Infinity Binary Pump User Manual
1Introduction
Pump Principle
Pump Principle
The Binary Pump is based on a two-channel, dual-piston in-series design
which comprises all essential functions that a solvent delivery system has to
fulfill. Metering of solvent and delivery to the high-pressure side are
performed by two pump assemblies which can generate pressure up to
1200 bar.
Each channel comprises a pump assembly including independent pump drive
for each piston, pump head, inlet valve, outlet valve, solvent heat exchanger
and an outlet filter. The two channels are fed into a low-volume mixing groove
in an automatic purge valve and a Jet Weaver mixer, with 35 or 100 µl volume
can be added downstream for optimum mixing performance.
A system pressure sensor, for monitoring the pump pressure, is attached to
the purge valve, normally connected in the B-channel of the pump, before the
mixing groove, in order to minimize delay volumes.

Agilent 1290 Infinity Binary Pump User Manual 13
Introduction 1
Pump Principle
Figure 1 The hydraulic path
In the user interface the solvent in use for each channel can be selected in
order to get optimum flow and composition accuracy. Although selecting the
right solvent is not required for low ripple, especially for composition accuracy
across pressure it is vital for the compensation algorithm to use the right
solvent properties. The density of the solvents is increased under the
influence of pressure and a certain displaced solvent will expand again when
the pressure is released, for example across the column. In order to achieve
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14 Agilent 1290 Infinity Binary Pump User Manual
1Introduction
Pump Principle
the correct volumetric flow while sample passes through the detector it is
necessary to correct for density related flow inaccuracies in the pump module.
In order to always deliver the best possible pressure stability, the pump
constantly tunes the delivery cycle for elasticity and dead volume effects. With
this feature the pump is able to deliver a stable and accurate flow without
requiring individual calibration settings. A further feature of the control and
compensation algorithm is leak correction. With this it is even possible to
compensate for minor leaks in primary pump chamber (inlet valve and seal),
without the performance of the pump being affected.
To increase the robustness of the pump it uses a fast defill function which
reduces the delivery time of the primary piston, thereby reducing the net
effects of leaks considerably. Furthermore each pump channel has only two
valves on its two pump heads which also reduce the potential of failures. In
order to reduce stresses on the pump hardware, it uses a smooth motion
control, which slowly increases or decreases the speed of the pistons to
prevent shock movements. In order to be able to control these movements a
high resolution encoder unit is attached to the pump drives which resolves a
revolution into 65000 steps, and each step translates to a volume of about
300 pl.

15
Agilent 1290 Infinity Binary Pump User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 16
Physical Specifications 19
Specifications 20
This chapter provides information on environmental requirements, physical and
performance specifications.

16 Agilent 1290 Infinity Binary Pump User Manual
2Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Consideration
The module power supply has wide ranging capabilities and accepts any line
voltage in the range mentioned in Table 1 on page 19. Consequently, there is
no voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened and the module is connected to power.
➔Make sure that it is always possible to access the power plug.
➔Remove the power cable from the instrument before opening the cover.
➔Do not connect the power cable to the Instrument while the covers are removed.
WARNING
Incorrect line voltage at the module
Shock hazard or damage of your instrument can result if the devices are connected
to line voltage higher than specified.
➔Connect your module to the specified line voltage.

Agilent 1290 Infinity Binary Pump User Manual 17
Site Requirements and Specifications 2
Site Requirements
Power Cords
Different power cords are offered as options with the module. The female end
of all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
CAUTION
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.
WARNING
Absence of ground connection or use of unspecified power cord
The absence of ground connection or the use of unspecified power cord can lead to
electric shock or short circuit.
➔Never operate your instrumentation from a power outlet that has no ground
connection.
➔Never use a power cord other than the Agilent Technologies power cord designed
for your region.
WARNING
Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

18 Agilent 1290 Infinity Binary Pump User Manual
2Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 19) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
WARNING
Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage of
electronic equipment.
➔Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
CAUTION
Condensation within the module
Condensation will damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

Agilent 1290 Infinity Binary Pump User Manual 19
Site Requirements and Specifications 2
Physical Specifications
Physical Specifications
Tab le 1 Physical Specifications
Type Specification Comments
Weight 21.8 kg (48 lbs)
Dimensions
(height × width × depth)
240 x 345 x 435 mm
(9.3 x 13.5 x 17 inches)
Line voltage 100 – 240 VAC, ± 10 % Wide-ranging
capability
Line frequency 50 or 60 Hz, ± 5 %
Power consumption 350 VA / 270 W / 922 BTU Maximum
Ambient operating
temperature
4–55 °C (41–131 °F)
Ambient non-operating
temperature
-40 – 70 °C (-4 – 158 °F)
Humidity < 95 % r.h. at 40 °C (104 °F) Non-condensing
Operating altitude Up to 2000 m (6562 ft)
Non-operating altitude Up to 4600 m (15091 ft) For storing the module
Safety standards:
IEC, CSA, UL
Installation category II, Pollution degree 2 For indoor use only.

20 Agilent 1290 Infinity Binary Pump User Manual
2Site Requirements and Specifications
Specifications
Specifications
Tab le 2 Performance specifications
Type Specification Comments
Hydraulic system Two dual pistons in series pumps
with proprietary servo-controlled
variable stroke design and smooth
motion control.
Settable flow range Setpoints 0.001 —2 ml/min, in
0.001 ml/min increments.
Executed in 300 pl/step
increments
Flow precision ≤0.07 % RSD or 0.005 min SD,
whatever is greater
(0.2 —2.0 ml/min).
Based on retention time at
constant room temperature.
Flow accuracy ±1 % or 10 µl/min, whatever is
greater.
Pumping degassed H2O at
10 MPa (100 bar)
Pressure operating
range
Operating pressure range 120 MPa
(1200 bar), up to 2 ml/min.
Pressure pulsation <1 % amplitude or < 0.5 MPa (5 bar),
whatever is greater.
At 1 ml/min water
Compressibility
compensation
Automatic, pre-defined, based on
mobile phase selection.
Gradient formation High pressure binary mixing.
Delay volume Jet Weaver V35: <45 µl
Jet Weaver V100: <75 µl
JetWeaver generally
recommended. For applications
requiring lowest delay volumes,
JetWeaver can be removed out of
the flow path. Delay volume is
then solely determined by the
volume of the connection
capillary.
Composition range Settable range: 0 – 100 % Recommended range: 1 — 99 %
or 5 µl/min per channel,
whatever is greater.