Agilent Technologies InfinityLab LC Series User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II SFC System
User Manual

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2017
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Manual Part Number
G4301-90104
Edition
02/2017
Printed in Germany
Agilent Technologies
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3
In This Guide...
In This Guide...
This manual covers the Agilent InfinityLab LC Series 1260 Infinity II SFC
System.
1 Introduction to Supercritical Fluid Chromatography (SFC)
This chapter provides an overview of the history, theory and benefits of SFC.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications only for the Agilent 1260 Infinity II SFC
System.
3 Installing the Agilent 1260 Infinity II SFC System
This chapter provides an overview of the installation and setup of the
hardware and software
4 Configuring the System
How to configure the Agilent 1260 Infinity II SFC System and Agilent 1260
Infinity II SFC Control Module in OpenLAB CDS.
5 Using the Agilent 1260 Infinity II SFC Control Module
This chapter provides information and hints on the use of the SFC System.
6 Maintenance and Repair
In this chapter only the SFC specific procedures are described. For procedures
similar to the Agilent module procedures, please refer to the single module
manuals (G4782A, G4767A, G7116A, G7115/65A)
7PartsforMaintenance
This chapter provides information on parts for maintenance and repair.

4
In This Guide...
8 Identifying Cables
This chapter provides information on cables used with the modules.
9 Appendix
This chapter provides addition information on safety, legal and web.

5
Contents
Contents
1 Introduction to Supercritical Fluid Chromatography (SFC) 7
History of SFC 8
Theory of SFC 9
Benefits of SFC 10
Common flow path overview for packed column SFC instrumentation 11
The Agilent 1260 Infinity II SFC System 12
2 Site Requirements and Specifications 21
Site Requirements 22
Specifications 26
3 Installing the Agilent 1260 Infinity II SFC System 31
Hardware Installation 32
4 Configuring the System 55
Configuring the SFC system in OpenLAB CDS 56
Setting up the Method 60
Status 62
Control 63
5 Using the Agilent 1260 Infinity II SFC Control Module 65
Powering up the Module 66
Power-up Sequence and Operational Control States 67
Operational Control States 68
Controlling the Agilent 1260 Infinity II SFC Control Module through the Agilent
OpenLAB CDS 71
Running a method on the SFC system 73
Shutting Down the SFC System 75

6
Contents
6 Maintenance and Repair 77
Inspection and Preventative Maintenance Intervals 78
General Maintenance procedures 80
7PartsforMaintenance 91
Agilent 1260 Infinity II SFC Control Module Parts 92
Agilent 1260 Infinity II SFC DAD Parts 94
8 Identifying Cables 95
Cable Overview 96
Analog Cables 98
Remote Cables 100
CAN/LAN Cables 104
RS-232 Cables 105
USB 106
9 Appendix 107
General Safety Information 108
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002-96-EC) 112
Radio Interference 113
Sound Emission 114
Solvent Information 115
Agilent Technologies on Internet 117

7
Agilent Technologies
1
Introduction to Supercritical Fluid
Chromatography (SFC)
History of SFC 8
Theory of SFC 9
Benefits of SFC 10
Common flow path overview for packed column SFC
instrumentation 11
The Agilent 1260 Infinity II SFC System 12
The Agilent 1260 Infinity II SFC Control Module (G4301A) 13
The Agilent 1260 Infinity II SFC Binary Pump (G4782A) 14
The Agilent 1260 Infinity II SFC Multisampler (G4767A) 15
The Agilent 1260 Infinity II Multicolumn Thermostat (G7116A) 16
The Agilent 1260 Infinity II Diode Array Detector WR (G7115A) and
Agilent 1260 Infinity II Multi Wavelength Detector (G7165A) 18
Applications 18
Columns 19
This chapter provides an overview of the history, theory and benefits of SFC.

8
1 Introduction to Supercritical Fluid Chromatography (SFC)
History of SFC
History of SFC
Supercritical fluid chromatography (SFC) was first introduced by Klesper et
al. in 1962 (Klesper, E.; Corwin, A. H.; Turner, D. A. J. Org. Chem. 1962,
27,700.) for the separation and analysis of a porphyrin mixture using open
tubular SFC. The first commercial instruments using packed columns were
available from Hewlett-Packard (HP) in 1982. Since then, several vendors have
developed and commercialized packed column SFC instrumentation for
analytical as well as for preparative separation. SFC is widely accepted for the
separation of chiral compounds and increased user interest has been observed
for a wide spectrum of small to medium sized molecules due to the analysis
speed achieved and the low solvent consumption.
The latest introduction of analytical SFC instrumentation, the Agilent 1260
Infinity II SFC Control Module coupled to an Agilent 1260 Infinity II Binary
LC system optimized for SFC.

9
Introduction to Supercritical Fluid Chromatography (SFC)
1
Theory of SFC
Theory of SFC
Figure 1 State of a solvent
The superior separation properties achieved by SFC can be explained best by
the thermodynamics of liquids and gases (see the phase diagram in Figure 1
on page 9). Above a critical pressure, liquids can no longer enter the gaseous
state; similarly, above a critical temperature, gases cannot be converted to
liquids. Above both the critical pressure and temperature (characterized by
the critical point), solvents are in the supercritical state. Under these
conditions, the mobile phases exhibit gaseous as well as liquid-like properties.
The major advantages of this state related to chromatography are improved
diffusion characteristics and mass transfer and low viscosity, which result
in high separation efficiency and fast separation capability.

10
1 Introduction to Supercritical Fluid Chromatography (SFC)
Benefits of SFC
Benefits of SFC
SFC is widely accepted for the analysis and separation of chiral compounds. In
addition, it gains increasing acceptance as a complementary liquid-based
separation technique to HPLC for high-throughput and high-resolution
analysis of complex mixtures. This is due to the thermodynamic properties of
supercritical fluids, which can be exploited for high throughput and high
efficiency. In addition, the mild thermal conditions also allow for the analysis
of thermally labile compounds. Typically, analysis times and column
re-equilibration are decreased by a factor of 3–5 compared to standard HPLC.
With the increasing costs of organic solvents and the environmental
awareness to minimize toxic waste, production SFC is increasingly accepted
as the “green alternative” to normal phase or reversed phase chromatography,
gaining popularity in method development and UV- and MS-based separation
and purification. A variety of parameters, such as stationary phase selection,
mobile phase composition, modifier type and concentration, column
temperature and system pressure, can be easily manipulated to fulfill
separation requirements by influencing, optimizing and exploiting selectivity
in SFC.