Agilent Technologies 2200 TapeStation User manual

Agilent Technologies
Agilent 2200 TapeStation
System
User Manual

Agilent 2200 TapeStation System - User Manual
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2015
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Manual Part Number
G2964-90003 Rev. C
Edition
09/2015
Printed in Germany
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Agilent 2200 TapeStation System - User Manual 3
Contents
Contents
1 Introduction to the 2200 TapeStation System 5
Overview of the System 6
2 Specifications 11
Technical Specifications 12
ScreenTape Specifications 14
3 Installing the System 23
Unpacking the System 24
Contents of the 2200 TapeStation System 26
Installing the System 30
4 Using the 2200 TapeStation System 33
Intended Use of the 2200 TapeStation System 35
Performance Limitations of Use 36
Additional Components Required by the User 37
Operating Procedure 38
How to prepare your samples 50
Good Measurement Practices for Analysing DNA on the Agilent 2200
Ta p e S ta t i o n 52
DNA Sample Preparation 53
RNA Sample Preparation 61
Protein Sample Preparation 67
5 Maintenance 71
General Information 72
Changing the Needle 73
Preventative Maintenance Interval 76
Ta p e S ta t i o n Te s t Ta p e 78

4 Agilent 2200 TapeStation System - User Manual
Contents
6 Appendix 83
Limited Use Label License 84
Sound Emission 85
Waste Electrical and Electronic Equipment Directive 86
Technical Service 87

5
Agilent 2200 TapeStation System - User Manual
Agilent Technologies
1
Introduction to the 2200 TapeStation
System
Overview of the System 6
ScreenTape Device Architecture 7
Agilent 2200 TapeStation Instrument Components 8
This chapter gives an introduction to the system

6 Agilent 2200 TapeStation System - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Overview of the System
The Agilent 2200 TapeStation system is a revolutionary automated
platform for simpler, faster and more reliable electrophoresis. It is made
up of three elements: a consumable tape (ScreenTape device), an
instrument (the 2200 TapeStation instrument), and an Agilent Software
package (2200 TapeStation Controller Software, and Agilent TapeStation
Analysis Software). The system is very straightforward to use, simply
place the sample tubes and ScreenTape device in to the 2200 TapeStation
instrument and let it load, separate, image, analyze, and present the
results.
This User Manual guides the operation of all ScreenTape assays, the 2200
TapeStation instrument, and software for the analysis of DNA, RNA, and
protein samples. The contents of the ScreenTape system are detailed
below.
Information pertaining to the 2200 TapeStation system can be found in:
•Technical Specification
•Agilent 2200 TapeStation Instrument Components
•Installing the System
Place ScreenTape device and some tips in the 2200 TapeStation
instrument.
Place your samples in the 2200 TapeStation instrument and click
Start on the 2200 TapeStation Controller Software.
View your analyzed results in around 1 min per sample.

Agilent 2200 TapeStation System - User Manual 7
Introduction to the 2200 TapeStation System 1
Overview of the System
Information pertaining to sample and ScreenTape device requirements can
be found in:
•ScreenTape Device Architecture
•Operating Procedure
ScreenTape Device Architecture
Figure 1 Example: P200 ScreenTape device
Barcode: The unique barcode tracks lane usage within each ScreenTape device and
allows traceability of results.
Buffer chamber: The buffer chamber is located at the top of the channel and contains
optimised buffers for the effective separation of nucleic acid fragments or
proteins.
Electrodes: The integrated electrodes apply a current across the ScreenTape device
and eliminate the need for any additional electrophoresis equipment.
Gel: The gel contained within the ScreenTape device has been developed
specifically to resolve nucleic acids or proteins.
ScreenTape device
product details:
The information is unique to each ScreenTape device. This includes:
ScreenTape assay type, product expiry date and a unique serial number.

8 Agilent 2200 TapeStation System - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Agilent 2200 TapeStation Instrument Components
Lid: The 2200 TapeStation instrument lid must be closed each time the 2200
TapeStation Controller Software is initialized, and whilst the instrument is
in operation.
LED: The LED will illuminate once the instrument is on. When the LED is
flashing slowly, the instrument is in use and the lid should not be opened,
rapid flashing indicates that the 2200 TapeStation instrument requires
attention.
Sample Block: There are 2 sample blocks provided that can either hold 0.2 mL sample
tube strips or a 96-well plate.
Tip Holder: The tip holder can accommodate up to 16 loading tips at any one time.
ScreenTape device: The ScreenTape device must be placed into the holder with the barcode
towards the front of the instrument, facing towards the right.
USB Socket: The USB connector is inserted into the USB socket to link the laptop to the
2200 TapeStation instrument.
Power-cable socket: The power cable must be connected to the 2200 TapeStation instrument
and the relevant mains electricity outlet.

Agilent 2200 TapeStation System - User Manual 9
Introduction to the 2200 TapeStation System 1
Overview of the System
Figure 2 2200 TapeStation instrument (front view)
Figure 3 2200 TapeStation instrument (back view)
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10 Agilent 2200 TapeStation System - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System