Agilent Technologies 2200 TapeStation User manual

Agilent Technologies
Agilent 2200 TapeStation
System
User Manual

Agilent 2200 TapeStation System - User Manual
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2015
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Manual Part Number
G2964-90003 Rev. C
Edition
09/2015
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Agilent 2200 TapeStation System - User Manual 3
Contents
Contents
1 Introduction to the 2200 TapeStation System 5
Overview of the System 6
2 Specifications 11
Technical Specifications 12
ScreenTape Specifications 14
3 Installing the System 23
Unpacking the System 24
Contents of the 2200 TapeStation System 26
Installing the System 30
4 Using the 2200 TapeStation System 33
Intended Use of the 2200 TapeStation System 35
Performance Limitations of Use 36
Additional Components Required by the User 37
Operating Procedure 38
How to prepare your samples 50
Good Measurement Practices for Analysing DNA on the Agilent 2200
Ta p e S ta t i o n 52
DNA Sample Preparation 53
RNA Sample Preparation 61
Protein Sample Preparation 67
5 Maintenance 71
General Information 72
Changing the Needle 73
Preventative Maintenance Interval 76
Ta p e S ta t i o n Te s t Ta p e 78

4 Agilent 2200 TapeStation System - User Manual
Contents
6 Appendix 83
Limited Use Label License 84
Sound Emission 85
Waste Electrical and Electronic Equipment Directive 86
Technical Service 87

5
Agilent 2200 TapeStation System - User Manual
Agilent Technologies
1
Introduction to the 2200 TapeStation
System
Overview of the System 6
ScreenTape Device Architecture 7
Agilent 2200 TapeStation Instrument Components 8
This chapter gives an introduction to the system

6 Agilent 2200 TapeStation System - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Overview of the System
The Agilent 2200 TapeStation system is a revolutionary automated
platform for simpler, faster and more reliable electrophoresis. It is made
up of three elements: a consumable tape (ScreenTape device), an
instrument (the 2200 TapeStation instrument), and an Agilent Software
package (2200 TapeStation Controller Software, and Agilent TapeStation
Analysis Software). The system is very straightforward to use, simply
place the sample tubes and ScreenTape device in to the 2200 TapeStation
instrument and let it load, separate, image, analyze, and present the
results.
This User Manual guides the operation of all ScreenTape assays, the 2200
TapeStation instrument, and software for the analysis of DNA, RNA, and
protein samples. The contents of the ScreenTape system are detailed
below.
Information pertaining to the 2200 TapeStation system can be found in:
•Technical Specification
•Agilent 2200 TapeStation Instrument Components
•Installing the System
Place ScreenTape device and some tips in the 2200 TapeStation
instrument.
Place your samples in the 2200 TapeStation instrument and click
Start on the 2200 TapeStation Controller Software.
View your analyzed results in around 1 min per sample.

Agilent 2200 TapeStation System - User Manual 7
Introduction to the 2200 TapeStation System 1
Overview of the System
Information pertaining to sample and ScreenTape device requirements can
be found in:
•ScreenTape Device Architecture
•Operating Procedure
ScreenTape Device Architecture
Figure 1 Example: P200 ScreenTape device
Barcode: The unique barcode tracks lane usage within each ScreenTape device and
allows traceability of results.
Buffer chamber: The buffer chamber is located at the top of the channel and contains
optimised buffers for the effective separation of nucleic acid fragments or
proteins.
Electrodes: The integrated electrodes apply a current across the ScreenTape device
and eliminate the need for any additional electrophoresis equipment.
Gel: The gel contained within the ScreenTape device has been developed
specifically to resolve nucleic acids or proteins.
ScreenTape device
product details:
The information is unique to each ScreenTape device. This includes:
ScreenTape assay type, product expiry date and a unique serial number.

8 Agilent 2200 TapeStation System - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Agilent 2200 TapeStation Instrument Components
Lid: The 2200 TapeStation instrument lid must be closed each time the 2200
TapeStation Controller Software is initialized, and whilst the instrument is
in operation.
LED: The LED will illuminate once the instrument is on. When the LED is
flashing slowly, the instrument is in use and the lid should not be opened,
rapid flashing indicates that the 2200 TapeStation instrument requires
attention.
Sample Block: There are 2 sample blocks provided that can either hold 0.2 mL sample
tube strips or a 96-well plate.
Tip Holder: The tip holder can accommodate up to 16 loading tips at any one time.
ScreenTape device: The ScreenTape device must be placed into the holder with the barcode
towards the front of the instrument, facing towards the right.
USB Socket: The USB connector is inserted into the USB socket to link the laptop to the
2200 TapeStation instrument.
Power-cable socket: The power cable must be connected to the 2200 TapeStation instrument
and the relevant mains electricity outlet.

Agilent 2200 TapeStation System - User Manual 9
Introduction to the 2200 TapeStation System 1
Overview of the System
Figure 2 2200 TapeStation instrument (front view)
Figure 3 2200 TapeStation instrument (back view)
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USB socket
On/off switch
Power-cable socket

10 Agilent 2200 TapeStation System - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System

11
Agilent 2200 TapeStation System - User Manual
Agilent Technologies
2
Specifications
Technical Specifications 12
ScreenTape Specifications 14
This chapter provides information on specifications.

12 Agilent 2200 TapeStation System - User Manual
2Specifications
Technical Specifications
Technical Specifications
2200 TapeStation instrument
Input voltage: 12 V DC
Power consumption: 40 W (VA)
Current: 3 A
Interface: USB cable (PC comms.)
Instrument Housing: UL94/VO rated flame retardant cast polyurethane
Dimensions: 400x 310x 310 mm
Weight: 12.5 kg
Power supply
Input voltage: 100 – 240 V AC
Input frequency: 50 – 60 Hz
Phase: 1
Current: 0.45 – 1.1 A
Environmental conditions
Optimal operating
temperature
20 °C (68 °F).
Instrument operating
temperature1
1Instrument operating temperature may be higher than ambient lab temperature, especially after
prolonged use.
12 – 37 °C (54 – 99 °F) for D1000 ScreenTape assay
17 – 37 °C (63 – 99 °F) for High Sensitivity D1000 ScreenTape assay
15 – 30 °C (59 – 86 °F) for Genomic DNA, D5000 and High Sensitivity
D5000 ScreenTape assays
14 – 30 °C (57 – 86 °F) for RNA and High Sensitivity RNA ScreenTape
assays
10 – 33 °C (50 – 91 °F) for P200 ScreenTape assay

Agilent 2200 TapeStation System - User Manual 13
Specifications 2
Technical Specifications
NOTE
If the instrument is out of the recommended temperature range for the ScreenTape assay
selected, the following error message will appear in the software:
•If the quoted current temperature is above the specified range, please move the
system out of direct sunlight and away from any windows. Check that any air
conditioning is functioning.
•If the quoted current temperature is below the specified range, please allow the
instrument to equilibrate to the ambient temperature, and avoid using in a cooled
area.

14 Agilent 2200 TapeStation System - User Manual
2Specifications
ScreenTape Specifications
ScreenTape Specifications
Specification (D1000 ScreenTape Assay)
Analytical Specifications D1000 ScreenTape assay and reagents
Sizing Range 35 – 1000 bp
Typical Resolution 35 – 300 bp: 15 %
300 – 1000 bp: 10 %
Sensitivity1
1signal-to-noise >3 (single peak)
0.1 ng/µL
Sizing Precision 5 % CV
Sizing Accuracy2
2Sizing Accuracy for electronic ladder: ±20%
±10 %
Quantitative Precision 0.1 – 1 ng/µL: 15 % CV
1 – 50 ng/µL: 10 % CV
Quantitative Accuracy3
3Measured against 2100 Bioanalyzer system
±20 %
Quantitative Range 0.1 – 50 ng/µL
Maximum sample buffer strength 20 mM KCl,
60 mM Phosphate Buffer,
60 mM Guanidine-HCl,
240 mM NaCl,
60 mM Acetate
Physical Specifications
Analysis Time 16 samples: <20 min
96 samples: ≈100 min
Samples per consumable 16
Sample volume required 1 µL
Kit stability 4 months
Kit size 112 samples

Agilent 2200 TapeStation System - User Manual 15
Specifications 2
ScreenTape Specifications
Specification (High Sensitivity D1000 ScreenTape Assay)
Analytical Specifications High Sensitivity D1000 ScreenTape assay and reagents
Sizing Range 35 – 1000 bp
Typical Resolution 35 – 300 bp: 15 %
300 – 1000 bp: 10 %
Sensitivity1
1signal-to-noise >3 (single peak)
5pg/µL
Sizing Precision 5 % CV
Sizing Accuracy2
2Sizing Accuracy for electronic ladder: ±20%
±10 %
Quantitative Precision 15 % CV
Quantitative Accuracy3
3Measured against 2100 Bioanalyzer system
±20 %
Quantitative Range 10 – 1000 pg/µL
Maximum sample buffer strength 7 mM KCl,
20 mM Phosphate Buffer,
20 mM Guanidine-HCl,
80 mM NaCl,
20 mM Acetate
Physical Specifications
Analysis Time 16 samples: <20 min
96 samples: ≈100 min
Samples per consumable 16
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples

16 Agilent 2200 TapeStation System - User Manual
2Specifications
ScreenTape Specifications
Specification (D5000 ScreenTape Assay)
Analytical Specifications D5000 ScreenTape assay and reagents
Sizing Range 100 – 5000 bp
Typical Resolution 400 – 5000 bp: 15 %
Sensitivity1
1signal-to-noise >3 (single peak)
0.1 ng/µL
Sizing Precision2
2Determined using the D5000 Ladder as sample
5%CV
Sizing Accuracy2,3
3Accuracy for run using single ScreenTape device
±10 %
Quantification Precision 0.1 – 1 ng/µL: 15 % CV
1 – 50 ng/µL: 10 % CV
Quantification Accuracy4
4Measured against 2100 Bioanalyzer system
±20 %
Quantification Range 0.1 – 50 ng/µL
Maximum Sample Buffer Strength 250 mM KCl,
250 mM Tris-HCl,
125 mM NaCl,
50 mM Acetate,
25 mM MgCl2,
25 mM BSA,
25 mM Guanidine-HCl
Physical Specifications
Multiple ScreenTape (96 well)
compatibility
Not compatible
Analysis Time 16 samples: <25 min
Samples per consumable 1 ladder + 15 samples
Sample volume required 1 µL
Kit stability 4 months
Kit size 112 samples/box

Agilent 2200 TapeStation System - User Manual 17
Specifications 2
ScreenTape Specifications
Specification (HS D5000 ScreenTape Assay)
Analytical Specifications D5000 ScreenTape assay and reagents
Sizing Range 100 – 5000 bp
Typical Resolution 400 – 5000 bp: 15 %
Sensitivity1
1signal-to-noise >3 (single peak)
5pg/µL
Sizing Precision2
2Determined using the High Sensitivity D5000 Ladder as sample
10 % CV
Sizing Accuracy33
3Accuracy for run using single ScreenTape device
±15 %
Quantification Precision 15 % CV
Quantification Accuracy4
4Measured against 2100 Bioanalyzer system
±25 %
Quantification Range 10 – 1000 pg/µL
Maximum Sample Buffer Strength 25 mM KCl,
25 mM Tris-HCl,
12.5 mM NaCl,
5 mM Acetate,
2.5 mM MgCl2,
2.5 mM BSA,
2.5 mM Guanidine-HCl
Physical Specifications
Multiple ScreenTape (96 well)
compatibility
Not compatible
Analysis Time 16 samples: <25 min
Samples per consumable 1 ladder + 15 samples
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples/box

18 Agilent 2200 TapeStation System - User Manual
2Specifications
ScreenTape Specifications
Specification (Genomic DNA ScreenTape Assay)
Analytical Specification Genomic DNA ScreenTape assay and reagents
Sizing Range 200 bp to > 60000 bp
Sensitivity 0.5 ng/µL
Sizing Precision1
1Determined using the Genomic DNA Ladder as sample
200 – 15000 bp: 15 % CV
Sizing Accuracy1200 – 15000 bp: ±15 %
Quantitative Precision2
2Average result from various genomic DNA sample types
15 % CV
Quantitative Accuracy3±20 %
Linear Concentration Range 10 – 100 ng/µL
DIN functional range 5 – 300 ng/µL
Physical Specification
Analysis Time 16 samples: < 25 min
96 samples: < 150 min
Samples per consumable 1 ladder + 15 samples
Sample Volume Required 1 µL
Shelf Life 4 months
Box/Kit size 112 samples/box

Agilent 2200 TapeStation System - User Manual 19
Specifications 2
ScreenTape Specifications
Specification (RNA ScreenTape Assay)
Analytical Specification RNA ScreenTape assay and reagents
Quality Score RINe
Quantitative Range 25 – 500 ng/µL
Quantitative Precision (%CV)15%
Quantitative Accuracy 20 %
Sizing Range 100 – 6000 nt
Sensitivity25ng/µL
RINefunctional range 25 – 500 ng/µL
Analysis Type Eukaryotic or Prokaryotic Total RNA QC
Maximum sample buffer strength 200 mM Tris,
20 mM EDTA, or
50 mM NaCl
Physical Specifications
Analysis Time 16 samples < 16 min
96 samples < 100 min
Samples per consumable 16
Sample volume required (µL) 1
Kit Stability 4 months
Kit Size 112 samples
For total RNA samples
1Within a ScreenTape
device
2Signal/noise >3 in water and TE

20 Agilent 2200 TapeStation System - User Manual
2Specifications
ScreenTape Specifications
Specification (High Sensitivity RNA ScreenTape Assay)
Analytical Specification HS RNA ScreenTape assay and reagents
Quality Score RINe
Quantitative Range 500 – 10000 pg/µL
Quantitative Precision (%CV)110 %
Quantitative Accuracy 30 %
Sizing Range 100 – 6000 nt
Sensitivity2100 pg/µL
RINefunctional range 1000 – 25000 pg/µL
Analysis Type Eukaryotic or Prokaryotic Total RNA QC
Maximum sample buffer strength 10 mM Tris
1mMEDTA
Physical Specifications
Analysis Time 16 samples < 30 min
96 samples < 180 min
Samples per consumable 16
Sample volume required (µL) 2
Kit Stability 4 months
Kit Size 112 samples
For total RNA samples
1Within a ScreenTape
device
2Signal/noise >3 in water and TE