Agilent Technologies Enhancer Cell User manual

Agilent Enhancer Cell
Operator’s Manual

Notices
Manual Part Number
70-9072 Rev B
February 2020
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© Agilent Technologies, Inc. 2020
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Enhancer Cell Operator’s Manual 3
Content
1 Introduction 5
Assembly Components 8
Unpacking Your Enhancer Cell Assembly 9
2 Operation 11
Membrane Selection Information for the Enhancer Cell 14
Ideal Characteristics for a Membrane 14
Examples of Membranes Used for In Vitro Release Tests 14

Content
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Enhancer Cell Operator’s Manual 5
1Introduction
Assembly Components 8
Unpacking Your Enhancer Cell Assembly 9

1Introduction
6 Enhancer Cell Operator’s Manual
The Enhancer Cell Assembly, designed for research, quality control, and product
development laboratories, is a PTFE cell with adjustable volume and a screw cap
to retain the skin or artificial membrane. The assembly can be used with any
dissolution tester and is available with 4.0, 2.0, or 0.5 cm² surface area. Using the
Paddle-over-Enhancer-Cell method (see Figure 3, “Paddle over Enhancer Cell,” on
page 7) provides release rates comparable to Franz Cell technology. It provides
release rate data for quality control of topicals using readily available dissolution
apparatus, including 200 mL or standard 900 mL dissolution vessels.
The alignment tool (see Figure 1, “Enhancer Cell Assembly,” on page 6) is used to
hold the membrane in place and ensure the washer is centered properly while
using the adjustment tool to set the volume with the adjustment plate. When
purchasing more than one Enhancer Cell Assembly, one alignment tool is
required per size. The adjustment tool is universal and fits all sizes. Both the
alignment tool and the adjustment tool are required and must be ordered
separately. The Enhancer Cell Assembly does not include the vessel, paddle, or
membrane / skin.
Figure 1. Enhancer Cell Assembly
*not provided—for illustrative purposes only.
Retaining Ring
Washer
Membrane or Skin *
Adjustment Plate
with O-ring Cell Body R
Alignment Tool
Adjustment Tool

1Introduction
Enhancer Cell Operator’s Manual 7
Figure 2. Enhancer Cell Assembly Surface Areas
Figure 3. Paddle over Enhancer Cell
0.5 cm² 2 cm² 4 cm²

1Introduction
8 Enhancer Cell Operator’s Manual
Assembly Components
The Enhancer Cell Assembly consists of the following components (see
Figure 1, “Enhancer Cell Assembly,” on page 6):
•Retaining ring – secures the membrane or skin sample to the cell body and
ensures full contact with the sample.
•Washer – provides a leak-proof seal between the membrane, retaining ring,
and cell body.
•Membrane (not provided) – retains the sample in the sample
compartment. Usually an artificial membrane although some tests use
skin samples.
•Adjustment plate – allows control of the volume of the reservoir within the
cell body. This threaded part can be placed at the appropriate height for
each test and can be completely removed for easy cleaning. A Viton O-ring
prevents leakage.
•Cell body – provides a variable depth reservoir for the sample (semisolid,
solution, suspension, or emulsion) being tested.
Two additional tools are required to properly use the Enhancer Cell Assembly and
must be ordered separately:
•Adjustment tool – to move the adjustment plate.
•Alignment tool – to ensure the membrane is in proper contact with the
sample and the washer is properly centered.
This product is warranted to be free from manufacturing defects at the time of
delivery. Please report any problems upon receipt of merchandise to an Agilent
Customer Care Center. Contact information can be found www.agilent.com
under your country using the Contact Us link.

1Introduction
Enhancer Cell Operator’s Manual 9
Unpacking Your Enhancer Cell Assembly
1Carefully remove the Enhancer Cell Assembly.
2Ensure all of the following items were shipped with your Enhancer Cell:
If any item is damaged or missing, contact the Dissolution Systems Service
Department or your local Agilent representative for replacements.
•Enhancer Cell Assembly Operator’s Manual
•Retaining ring
•Washer
•Adjustment plate with O-ring
•Cell body
•Included if purchased separately:
•Adjustment tool
•Alignment tool

1Introduction
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