Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Thermostatted Column
Compartment
User Manual

Agilent 1260 Infinity TCC User Manual
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Manual Part Number
G1316-90013
Edition
06/2010
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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For Research Use Only

Agilent 1260 Infinity TCC User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1260 Infinity Thermostatted Column
Compartments (G1316A TCC).
1 Introduction to the Column Compartment
This chapter gives an introduction to the TCC, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information about site requirements and specifications
for the module.
3 Installing the Column Compartment
This chapter describes the installation of the thermostatted column
compartment.
4 How to optimize the Column Compartment
This chapter provides information on how to optimize the thermostatted
column compartement.
5 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7TestFunctions
This chapter describes the TCC’s built in test functions.

4 Agilent 1260 Infinity TCC User Manual
In This Guide…
8 Maintenance
This chapter describes the maintenance and repair of the TCC.
9 Parts for Maintenance
This chapter provides information on parts for maintenance.
10 Identifying Cables
This chapter provides information on cables used with the 1260 Infinity series
of HPLC modules.
11 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity TCC User Manual 5
Contents
Contents
1 Introduction to the Column Compartment 9
Main Features 10
System Overview 11
Column-Identification System 13
Column Switching Valve (Optional) 15
Instrument Layout 18
Electrical Connections 19
Interfaces 21
Setting the 8-bit Configuration Switch (without On-Board LAN) 27
2 Site Requirements and Specifications 33
Site Requirements 34
Physical Specifications 37
Performance Specifications 38
3 Installing the Column Compartment 39
Unpacking the Column Compartment 40
Optimizing the Stack Configuration 41
Installing the Column Compartment 46
Flow Connections of the Column Compartment 49
Placing Columns 53
4 How to optimize the Column Compartment 55
Optimizing the Performance of your Column Compartment 56
5 Troubleshooting and Diagnostics 57
Overview of the Module’s Indicators and Test Functions 58
Status Indicators 59
Available Tests depending on User Interfaces 61
Agilent Lab Advisor Software 62

6 Agilent 1260 Infinity TCC User Manual
Contents
6 Error Information 63
What Are Error Messages 64
General Error Messages 65
TCC Error Messages 70
7TestFunctions 77
Thermostat Function Test 78
Pressure Test 81
Column Thermostat Temperature Calibration 82
8 Maintenance 91
Warnings and Cautions 92
Introduction to Maintenance 94
Overview of Maintenance 95
Cleaning the Module 96
Changing Column Identification Tags 97
Replacing Head Parts of Column Switching Valve 99
Correcting Leaks 102
Replacing the Module’s Firmware 103
9 Parts for Maintenance 105
Valve Options Overview 106
Column Switching Valve 2 Position/6 Port 107
Micro Column Switching Valve 2 Position-6 Port 109
Micro Column Switching Valve 2 Position/10 Port 110
Capillary Kit Column Regeneration 111
Column Regeneration Kit 113
Accessory Kits 115
10 Identifying Cables 117
Cable Overview 118
Analog Cables 120
Remote Cables 122
BCD Cables 125
CAN/LAN Cables 127
External Contact Cable 128

Agilent 1260 Infinity TCC User Manual 7
Contents
Agilent Module to PC 129
Agilent 1200 Module to Printer 130
11 Appendix 131
Safety Symbols 132
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 135
Lithium Batteries Information 136
Radio Interference 137
Sound Emission 138
Use of Solvents 139
Agilent Technologies on Internet 140

8 Agilent 1260 Infinity TCC User Manual
Contents

9
Agilent 1260 Infinity TCC User Manual
Agilent Technologies
1
Introduction to the Column
Compartment
Main Features 10
System Overview 11
Column-Identification System 13
Column Switching Valve (Optional) 15
Instrument Layout 18
Electrical Connections 19
Serial Number Information 1260 Infinity 19
Electrical Connections 19
Interfaces 21
Overview Interfaces 23
Setting the 8-bit Configuration Switch (without On-Board LAN) 27
Communication Settings for RS-232C 28
Special Settings 30
This chapter gives an introduction to the TCC, instrument overview and internal
connectors.

10 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Main Features
Main Features
The Agilent 1260 Infinity Thermostatted Column Compartment is a stackable
temperature-controlled column compartment for LC. It is used for heating and
cooling to meet extreme requirements of retention time reproducibility.
The main features are:
•Peltier heating and cooling from 10 degrees below ambient up to 80 °C with
high heating and cooling speeds for maximum application flexibility and
stability.
•Holds up to three 30 cm columns and optimized design gives minimum dead
volumes and maximum efficiency.
•Two independently programmable heat exchangers contribute volumes of
only 3 µL and 6 µL.
•Electronic column-identification module as standard for GLP
documentation of column type and major column parameters.
•Optional high-quality Rheodyne®column switching valves with ceramic
stator-face assemblies for prolonged lifetime.
For specifications, see “Performance Specifications” on page 38.

Agilent 1260 Infinity TCC User Manual 11
Introduction to the Column Compartment 1
System Overview
System Overview
The Concept of Heating and Cooling
The design of this thermostatted column compartment uses column heating
and cooling devices with Peltier elements. The solvent entering the column
compartment is heated up or cooled down to a settable temperature with two
low-volume heat exchangers (3 µl on left side, 6 µl on right side), made of a
short piece of capillary 0.17 mm i.d. leading through a heat exchanger. The
heat exchanger is designed such that it can function simultaneously as an air
heater. The shape of the heat exchanger surface allows the area around the
column to be kept at a similar temperature level as the liquid running through
the column. This is done by thermal convection and radiation between the
heat exchanger fins. This design ensures that the column and the solvent
flowing through it are almost at the same temperature.
Actual temperature control is accomplished at the heat exchanger. The solvent
cools down or heats up on its transfer from the heating block to the column
inlet. This depends on several factors: flow rate, setpoint temperature,
ambient temperature and column dimensions.
In a flow-through temperature regulation system, there are necessarily slightly
different temperatures at different positions. If, for example, the temperature
set by the user is 40 °C, then the heat exchanger is regulated to a temperature
40.8 °C which is different by a certain offset (here 0.8 °C). The solvent
temperature at the column entry would be about 39 °C.
The actual temperature displayed on the user interface is always the derived
temperature taken at the heat exchanger, corrected by the offset explained
above.
Any type of heated column compartment brings one important consequence
for column temperature equilibration. Before an equilibrium is reached, the
whole mass of column, column packing, and solvent volume inside the column
has to be brought to the selected temperature. This depends on several factors:
flow rate, setpoint temperature, ambient temperature and column dimensions.
The higher the flow rate, the faster the column equilibrates (due to
thermostatted mobile phase).

12 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
System Overview
“Column Thermostat Temperature Calibration”on page 82 shows a setpoint
temperature of 40 °C. Some time after entering the setpoint the heat
exchanger has reached its temperature and the control activity starts. The
TEMPERATURE NOT READY signal will be cancelled 20 seconds after the sensed
temperature was within a range of ± 0.5 °C of the setpoint (other values can be
set via the user interface). However this does not necessarily mean that the
column has already reached the correct temperature. The equilibration of the
column may take longer. Stability of the pressure signal is a good indication
for equilibrium.
Figure 1 Equilibration of Heat Exchanger and Column Temperature
The temperature calibration and verification is described in the Service
Manual.
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Agilent 1260 Infinity TCC User Manual 13
Introduction to the Column Compartment 1
Column-Identification System
Column-Identification System
The Agilent 1260 Infinity Thermostatted Column Compartment is equipped
with a column-identification system. It allows to read and write
column-specific information to and from the column-identification tag.
Figure 2 Column-Identification System
Table 1 on page 13 shows the information that can be stored. The information
fields can be edited via the user interface.
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Table 1 Column-Identification Module Information
Item Example Comment
Product number 79916OD-552
Serial number 950522 Date of manufacturing
Batch number 1675
Geometry 100 mm × 2.1 mm
Stationary phase ODS Hypersil
Particle size 10 µm
Number of injections 1267 See below.
Maximum pressure allowed 400 bar

14 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Column-Identification System
The number of injections will be updated each run to create a column lifecycle
(history). The user interface allows to edit all information.
Maximum temperature recommended 70 °C
Maximum pH recommended 12
Column void volume
Table 1 Column-Identification Module Information
Item Example Comment
NOTE If a column switching valve (see “Column Switching Valve (Optional)” on page 15) is
installed in the module, the update of the number of injections depends on the position of
the column switching valve. For example, if the left column is selected, the right column is
not updated, and vice versa. If no column switching valve is installed both sides are
updated at the same time.

Agilent 1260 Infinity TCC User Manual 15
Introduction to the Column Compartment 1
Column Switching Valve (Optional)
Column Switching Valve (Optional)
Figure 3 Location of Column Switching Valve
Two Column Selection
The valve can select either column 1 or column 2. The offline column is sealed
by connecting head to tail. Switching should be done when the flow is off and
the pressure is zero.
NOTE Before switching the valve, switch off the pump or set the flow to zero. Keeping the flow on
while the valve is switched can cause exceeding the maximum pressure. This will stop
method or sequence execution.

16 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Column Switching Valve (Optional)
Figure 4 Column 1 Active
Figure 5 Column 2 Active
Precolumn Back-flushing
The sample is injected into series-connected precolumn and analytical
column. After the valve has switched, the analytical column flow continues in
normal direction. Only the precolumn is back-flushed, eluting highly retained
peaks directly to the detector.
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Agilent 1260 Infinity TCC User Manual 17
Introduction to the Column Compartment 1
Column Switching Valve (Optional)
Figure 6 Precolumn Back-flushing
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18 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Instrument Layout
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers of foam plastic spacers in which the mechanical
and electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
•virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
•the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
•the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
•the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.

Agilent 1260 Infinity TCC User Manual 19
Introduction to the Column Compartment 1
Electrical Connections
Electrical Connections
•The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer and
synchronization.
•One analog output provides signals for integrators or data handling
systems.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
•With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
•The power input socket accepts a line voltage of 100 – 240 VAC ± 10 % with a
line frequency of 50 or 60 Hz. Maximum power consumption varies by
module. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
Serial Number Information 1260 Infinity
The serial number information on the instrument labels provide the following
information:
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.
CCXZZ00000 Format
CC Country of manufacturing (DE Germany)
X Alphabetic character A-Z (used by manufacturing)

20 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Electrical Connections
Figure 7 Rear View of the Thermostatted Column Compartment
ZZ Alpha-numeric code 0-9, A-Z, where each combination
unambiguously denotes a module (there can be more than one
code for the same module)
00000 Serial number
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NOTE The GPIB interface has been removed with the introduction of the 1260 Infinity modules.