Agilent Technologies 990 User manual

Agilent 990
Micro Gas Chromatograph
User Manual

Notices
© Agilent Technologies, Inc. 2022
No part of this manual may be reproduced in
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eign language) without prior agreement and
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tional copyright laws.
Manual Part Number
G3588-90010
Edition
Third edition, August 2022
Printed in USA
Agilent Technologies, Inc.
412 Ying Lun Road
Waigoaqiao Free Trade Zone
Shanghai 200131 P.R.China
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The material contained in this document is
provided “as is,” and is subject to being
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Further, to the maximum extent permitted by
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed
or adhered to, could result in
damage to the product or loss of
important data. Do not proceed
beyond a CAUTION notice until the
indicated conditions are fully
understood and met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed
or adhered to, could result in
personal injury or death. Do not
proceed beyond a WARNING notice
until the indicated conditions are
fully understood and met.

Agilent 990 Micro GC User Manual 3
Contents
1Introduction
Safety Information 10
Important safety warnings 10
Hydrogen safety 10
Safety symbols 11
Safety and regulatory information 12
General safety precautions 12
Shipping Instructions 14
Cleaning 14
Instrument Disposal 14
2 Instrument Overview
Principle of Operation 16
Front View 17
Front inside view 18
Rear View 19
Rear inside view 21
Communication Ports 23
Connecting a Channel Extension Cabinet 24
Carrier Gas Connection 26
Power 27
Power source 27
Power requirements 27
Disposal 28
Specifications 28
Ambient Pressure 29
Ambient Temperature 29
Maximum Operation Altitude 29
Storage requirements 29
Micro GC Cycle with Constant Pressure 30
Micro GC Cycle with Ramped Pressure 31
3 Installation and Use
Pre-Installation Requirements 34
Inspect the Shipping Packages 34

4Agilent 990 Micro GC User Manual
Unpack the Micro GC 34
Review the Packing List 35
Agilent 990 Micro GC Installation 36
Step 1: Connect carrier gas 36
Step 2: Connect to calibration gas or checkout sample 36
Step 3: Install power supply 36
Step 4: Connect to computer or local network 37
Step 5: Install the Chromatography Data System 37
Step 6: Assign an IP address 37
Step 7: Complete Agilent 990 Micro GC configuration in the Chromatography Data
System 38
Create the Test Method 39
Perform a Series of Runs 40
Shut Down Procedure 41
Long Storage Recovery Procedure 41
4 Sample Gas Handling
Installing Sample Inlets 44
Installing and configuring a sample inlet manifold 44
Sample inlet configurations 46
Using the External Filter Unit 47
Heated Zones 48
Connecting a Sample to the 990 Micro GC 49
Front inlet 49
Connecting sample inlet accessories 49
990 Micro GC Optional Pressure Regulators 50
G3588-68863 50
G3588-68864 53
Manual Injection 56
Manual injection guidelines 56
Injection procedure 56
Manual injection flow diagram 58
Manual injection kits 58
5 Micro GC Channels
Carrier Gas 62
Dynamic Electronic Gas Control (DEGC) 63
Inert Sample Path 63

Agilent 990 Micro GC User Manual 5
Injector 63
Column 64
Molsieve 5Å columns 65
CP-Sil 5 CB columns 66
CP-Sil 13 CB and CP-Sil 19 CB columns 67
PoraPlot 10 m column 68
HayeSep A 40 cm heated column 69
COXand Al203/KCI columns 70
Volamine Column 71
MES (NGA) and CP-WAX 52 CB columns 72
Column conditioning 73
Replacing Channel Column, Injector Die, and TCD 74
Backflush Option 75
Tuning the backflush time (except on a HayeSep A channel) 77
Tuning the backflush time on a HayeSep A channel 78
Backflush to Detector 79
CP-Sil 5 CB Backflush to detector 79
Al2O3 Backflush to detector 79
Tuning the backflush time 80
To disable backflush 81
Set invert signal time 82
Checkout information 83
C6+ Calorific value calculation 84
TCD Detector 84
6 Channel Exchange and Installation
Replacement Procedure for Micro GC Channel 86
Tools required 86
Channel removal procedure 86
Channel installation procedure 91
7 Replacement Procedures
Removing the Covers 94
Removing the front cover 94
Removing the rear covers 95
Sample Line Replacement 97
Tools required 97
Replacement procedure for Micro GC sample line 97
RTS Filter Replacement 100
Tools required 100

6Agilent 990 Micro GC User Manual
Replacement procedure for Micro GC RTS filter 100
Septum Replacement for Manual Injection 103
Septum injection port 103
Genie Filter Membrane Replacement 104
Tools required 104
Replacement procedure for Genie Filter membrane 104
Connecting the External LCD 106
LCD touch screen 106
8Communications
Access the Connection Ports 112
990 Chromatography Data Systems 115
Ethernet Networks 116
IP Addresses 116
Example network configurations 117
Subnet 1 119
Subnet 2 119
USB Wi-Fi 120
Frequently Asked Questions (FAQ) 121
Glossary of network terms 121
External I/O 123
9Errors
Error Handling 128
Error List 129
10 Micro GC Biogas Analyzers
Biogas Analyzer 135
Biogas Analyzer Extended 135
Checkout Information 136
CP-Molsieve channel 137
CP-PoraPLOT U channel 138
CP Sil 5 CB channel 139
Certificate of the Universal Gas Calibration Standard 140
The Biogas Analyzer Method 141
11 Micro GC Natural Gas Analyzer
Agilent 990 Micro GC - Natural Gas Analyzers 144

Agilent 990 Micro GC User Manual 7
Introduction 144
Types of Agilent 990 Micro GC - Natural Gas Analyzers 144
Agilent 990 Micro GC - Natural Gas Analyzer A and the Extended Version 144
Agilent 990 Micro GC - Natural Gas Analyzer B and the Extended Version 145
Checkout Information 146
Introduction 146
Natural Gas Analyzer A and the A Extended Version 147
Natural Gas Analyzer B and B Extended Version 154
Certificate of the NGA Gas Calibration Standard 160
Certificate of the Universal Gas Calibration Standard 161
Typical Method Settings for Natural Gas Analyzers 162
Carrier Gas Type Configuration 164
Procedure to change the carrier gas type 165
Method for CP-Molsieve 5A Channel with Carrier Gas Argon 168

8Agilent 990 Micro GC User Manual

Agilent 990 Micro GC User Manual 9
1Introduction
Safety Information 10
Shipping Instructions 14
Cleaning 14
Instrument Disposal 14
This chapter provides important information about using the Agilent 990 Micro Gas
Chromatograph, Micro GC, safely. To prevent any injury to you or any damage to the instrument, it
is essential that you read the information in this chapter.

1Introduction
Safety Information
10 Agilent 990 Micro GC User Manual
Safety Information
Important safety warnings
There are several important safety notices that you should always keep in mind when using the
Micro GC.
WARNING
When handling or using chemicals for preparation or use within the Micro GC, all applicable local
and national laboratory safety practices must be followed. This includes, but is not limited to,
correct use of Personal Protective Equipment, correct use of storage vials, and correct handling
of chemicals, as defined in the laboratory’s internal safety analysis and standard operating
procedures. Failure to adhere to laboratory safety practices could lead to injury or death.
Hydrogen safety
Hydrogen is a commonly used GC carrier gas. When mixed with air, hydrogen can form explosive
mixtures and has other dangerous characteristics.
WARNING
When using hydrogen (H2) as the carrier gas, be aware that hydrogen gas can create a fire or
explosion hazard. Ensure that the supply is turned off until all connections are made.
Hydrogen is flammable. Leaks, when confined in an enclosed space, may create a fire or
explosion hazard. In any application using hydrogen, leak test all connections, lines, and valves
before operating the instrument. Always turn off the hydrogen supply at its source before
working on the instrument.
•Hydrogen is combustible over a wide range of concentrations. At atmospheric pressure,
hydrogen is combustible at concentrations from 4 % to 74.2 % by volume.
•Hydrogen has the highest burning velocity of any gas.
•Hydrogen has a very low ignition energy.
•Hydrogen that is allowed to expand rapidly from high pressure into the atmosphere can
self-ignite.
•Hydrogen burns with a nonluminous flame which can be invisible under bright light.

1Introduction
Safety symbols
Agilent 990 Micro GC User Manual 11
Safety symbols
Warnings in the manual or on the instrument must be observed during all phases of operation,
service, and repair of this instrument. Failure to comply with these precautions violates safety
standards of design and the intended use of the instrument. Agilent Technologies assumes no
liability for the customer’s failure to comply with these requirements.
See accompanying instructions for more information.
Indicates a hot surface.
Indicates hazardous voltages.
Indicates earth (ground) terminal.
Indicates potential explosion hazard.
Indicates electrostatic discharge hazard.
Indicates a hazard. See the Agilent 990 GC user documentation for the item
labeled.
Indicates that you must not discard this electrical/electronic product in
domestic household waste

1Introduction
Safety and regulatory information
12 Agilent 990 Micro GC User Manual
Safety and regulatory information
This instrument and its accompanying documentation comply with the CE specifications and the
safety requirements for electrical equipment for measurement, control, and laboratory use
(CEI/IEC 1010-1)CCSAUS and FCC-b.
This device has been tested and found to comply with the limits for a Class A digital device,
pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used
in accordance with the instruction manual, may cause harmful interference to radio
communications.
Operation of this equipment in a residential area is likely to cause harmful interference, in which
case the user will be required to correct the interference at his own expense.
NOTE
This instrument has been tested per applicable requirements of EMC Directive as required to
carry the European Union CE Mark. As such, this equipment may be susceptible to
radiation/interference levels or frequencies, which are not within the tested limits.
General safety precautions
To ensure safe equipment operation, follow these safety practices:
•Perform periodic leak checks on all supply lines and pneumatic plumbing.
•Do not allow gas lines to become kinked or punctured.
•Place lines away from foot traffic and extreme heat or cold.
•Store organic solvents in fireproof, vented, and clearly labeled cabinets so they are easily
identified as either toxic, flammable, or both.
•Do not accumulate waste solvents. Dispose of such materials through a regulated disposal
program and not through municipal sewage lines.
WARNING
This instrument is designed for chromatographic analysis of appropriately prepared samples. It
must be operated using appropriate gases or solvents and within specified maximum ranges for
pressure, flows, and temperatures as described in this manual. If the equipment is used in a
manner not specified by the manufacturer, the protection provided by the equipment may be
impaired.
WARNING
It is the responsibility of the customer to inform Agilent customer support representatives if the
instrument has been used for the analysis of hazardous samples, prior to any instrument service
being performed or when an instrument is being returned for repair.
•Avoid exposure to potentially dangerous voltages. Disconnect the instrument from all power
sources before removing protective panels.
•When it is necessary to use a nonoriginal power cord and plug, ensure the replacement cord
adheres to the color coding and polarity described in the manual and all local building safety
codes.
•Replace faulty or frayed power cords immediately with the same type and rating.
•Place this instrument in a location with sufficient ventilation to remove gases and vapors.
•Ensure there is enough space around the instrument for it to cool off sufficiently.

1Introduction
General safety precautions
Agilent 990 Micro GC User Manual 13
•Before plugging the instrument in or turning the power on, always ensure that the voltage is
set appropriately for your local power source.
•Do not turn on the instrument if there is a possibility of any kind of electrical damage. Instead,
disconnect the power cord and contact your local Agilent sales office.
•The supplied power cord must be inserted into a power outlet with a protective ground
connection. When using an extension cord, ensure that the cord is also properly grounded.
•Do not change any external or internal grounding connections, as this could endanger you or
damage the instrument.
•The instrument is properly grounded when shipped. You do not need to make any changes to
the electrical connections or to the instrument chassis to ensure safe operation.
•When working with this instrument, follow the regulations for Good Laboratory Practices
(GLP). Wear safety glasses and appropriate clothing.
•Do not place containers with flammable liquids on this instrument. Spilling liquid over hot
parts may cause fire.
•This instrument may use flammable or explosive gases, such as hydrogen gas under
pressure. Before operating the instrument, know and follow prescribed operation procedures
for the gases you use.
•Never try to repair or replace any component that is not described in this manual without the
assistance of an Agilent service engineer. Unauthorized repairs or modifications will result in
rejection of warranty claims.
•Always disconnect the AC power cord before attempting any type of maintenance.
•To prevent danger to you or damage to the instrument, use proper tools when working on the
instrument.
•Do not attempt to replace any battery in this instrument, other than as specified in the manual.
•Damage can result if the instrument is stored under unfavorable conditions for prolonged
periods. (For example, damage will occur if stored while subject to heat, water, or other
conditions exceeding the allowable operating conditions).
•This unit has been designed and tested in accordance with recognized safety standards and is
designed for use indoors.
•If the instrument is used in a manner not specified by the manufacturer, the protection
provided by the instrument may be impaired.
•Substituting parts or performing any unauthorized modification to the instrument may result
in a safety hazard.
•Changes or modifications not expressly approved by the responsible party for compliance
could void the user's authority to operate the equipment.

1Introduction
Shipping Instructions
14 Agilent 990 Micro GC User Manual
Shipping Instructions
If your Micro GC must be shipped for any reason, it is very important to always include the power
supply.
Cleaning
To clean the surface of the Micro GC:
1Switch the Micro GC off.
2Remove the power cable.
3Put protection plugs on the sample inlets.
4Put protection plugs on the column vents.
5Use a soft brush (not hard or abrasive) to carefully brush away all dust and dirt.
6Use a soft, clean cloth dampened with mild detergent to clean the outside of the instrument.
•Never clean the inside of the instrument.
•Never use alcohol or thinners to clean the instrument; these chemicals can damage the
case.
•Do not to get water on the electronic components.
•Do not use compressed air to clean the instrument.
Instrument Disposal
When the Micro GC or its parts have reached the end of their useful life, dispose of them in
accordance with the environmental regulations that are applicable in your country.

Agilent 990 Micro GC User Manual 15
2Instrument Overview
Principle of Operation 16
Front View 17
Rear View 19
Communication Ports 23
Connecting a Channel Extension Cabinet 24
Carrier Gas Connection 26
Power 27
Ambient Pressure 29
Ambient Temperature 29
Maximum Operation Altitude 29
Micro GC Cycle with Constant Pressure 30
Micro GC Cycle with Ramped Pressure 31
The Agilent 990 Micro GC uses GC channels, each of which consists of a Dynamic Electronic Gas
Control (DEGC) injector, column, and detector.
The Micro GC is a self-contained package with all of the normal GC components. It is a dual
channel cabinet (one or two GC channels). Two cabinets can be connected to form a quad
channel system (up to four GC channels). A computer with a chromatography data system (CDS)
is needed to complete the system.
This chapter provides a brief overview of the Agilent 990 Micro GC.

2 Instrument Overview
Principle of Operation
16 Agilent 990 Micro GC User Manual
Principle of Operation
The Agilent 990 Micro GC can be equipped with one or two independent column channels. Each
column channel is a complete, miniaturized GC with dynamic electronic gas control,
micro-machined injector, narrow-bore analytical column, and micro thermal conductivity detector
(µ TCD), Figure 1.
Each Agilent 990 Micro GC cabinet has two analytical channel slots. Two cabinets can be
combined to support four analytical channels simultaneously. For a three or four channel
configuration, a channel extension cabinet can be connected to the Micro GC.
The Agilent 990 Micro GC analytical channels can optionally be equipped with a backflush option.
The advantages include the protection of the stationary column phase against moisture and
carbon dioxide. Also, the backflush option results in shorter analysis times as late elution
compounds, which are not of interest, do not enter the analytical column.
Figure 1. Agilent 990 Micro GC setup
Micro-machined
injector
Narrow-bore
analytical column
Micro thermal
conductivity
detector
Dynamic
electronic gas
control
One to four
independently
controlled
micro-machined
GC channels

2 Instrument Overview
Front View
Agilent 990 Micro GC User Manual 17
Front View
The LED indicator light has color states as described in Table 1.
Figure 2. Front view of the Agilent 990 Micro GC
Table 1 LED state definitions
LED state Indication
Red - solid Recoverable error.
Red - blinking Critical error
Yellow - solid System in not ready or stabilizing status.
Yellow - blinking System in flushing or column cleaning status.
Green - solid System in ready status:
All heated zone, column head pressure, and TCD statuses are within ready window.
Green - blinking System in run status.
LED indicator light
USB ports
Primary front
sample inlet
Power button
with LED light
Optional secondary
front sample inlet
(covered)

2 Instrument Overview
Front inside view
18 Agilent 990 Micro GC User Manual
Front inside view
Unscrew and remove the front cover to view the channel cases and pump box.
See Removing the Covers on page 94.
Figure 3. Front inside view of the Agilent 990 Micro GC
Top assembly
Bottom assembly
Channel 1
Channel locking tabs
Pump box
Channel locking tabs
Channel 2
Sample inlet manifold
Second sample
manifold mount
Sample inlet
Sample inlet
Lock bar
Channel 2
Lock bar

2 Instrument Overview
Rear View
Agilent 990 Micro GC User Manual 19
Rear View
Figure 4. Rear view of the Agilent 990 Micro GC
Figure 5. Rear communication ports of the Agilent 990 Micro GC top assembly
Rear communication ports
(top assembly)
CAN connection
between the GC and
Channel Extension Cabinet
Power connectors
Combined carrier gas
and vent plate
(Figure 6 shows
in detail.)
USBRS-232
(COM-1)
Ethernet
(LAN)
Digital and Analog I/O

2 Instrument Overview
Rear View
20 Agilent 990 Micro GC User Manual
Figure 6. Combined carrier gas and vent plate
Carrier 1 Backflush vent Upper channel
vent
Sample vent
Carrier 2 Lowerchannel
vent