Agilent Technologies AssayMAP Bravo Platform User manual

AssayMAP Bravo Platform
Error Recovery Guide
Original Instructions

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Manual Part Number
D0021233 Revision A
October 2022
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iiiAssayMAP Bravo Platform Labware Reference Guide
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
About this guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi
Reporting problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
2. Common startup error messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Unable to communicate with the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
There appears to be a plate present . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Verify that it is safe to home the W-axis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Accessory startup errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3. Common AssayMAP Bravo error messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Could not dock gripper and other communication errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Robot disable is active messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
W-axis was commanded to an invalid destination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4. Miscellaneous errors and recovery techniques. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
W-axis is not homed error message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Using Bravo Diagnostics to reclaim reagents from syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Pausing and continuing a run in the workbench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Contents
iviv AssayMAP Bravo Platform Labware Reference Guide
This page is intentionally blank.

v
Preface
This guide provides a reference for how to resolve error messages that can occur
when using the Agilent Protein Sample Prep Workbench.
This section contains the following topics:
•“About this guide” on page vi
•“Reporting problems” on page viii

Preface
About this guide
vi AssayMAP Bravo Platform Error Recovery Guide
About this guide
Overview
This guide provides a reference to common error messages you may encounter when
using the AssayMAP Bravo Platform.
Software version
This guide documents the following software versions:
•Protein Sample Prep Workbench 4.0
•VWorks Automation Control 14.1.1
What is new in this revision
Item See...
Added a section on error
messages that occur during
startup.
“Common startup error messages” on page 1
Updated the topics for
Workbench 4.0.
“Pausing and continuing a run in the workbench”
on page 23.

vii
Preface
About this guide
AssayMAP Bravo Platform Error Recovery Guide
Accessing the user guides
You can find the user guides for the AssayMAP applications and utilities in the Protein
Sample Prep Workbench.
The Literature Library provides links to the user guides.
The app quick start guides are available in the App Library and additional utility guides
are available in the Utilities Library.

Preface
Reporting problems
viii AssayMAP Bravo Platform Error Recovery Guide
Reporting problems
If you find a problem with the AssayMAP Bravo Platform, contact Agilent Technical
Support. For contact information, go to https://www.agilent.com/en/contact-us/page.
To report problems with… Have the following information ready
Hardware •Instrument serial number from the Bravo serial
number label
•Short description of the problem
Software •Instrument serial number from the Bravo serial
number label
•Short description of the problem
•Relevant software version number (for example,
automation control software, diagnostics software,
and firmware)
•Error message text (or screen capture of the error
message dialog box)
•Relevant files, such as log files
AssayMAP Cartridges •Cartridge type
•Lot number
•Short description of the problem

1
2 Common startup error messages
This chapter describes error messages that you may encounter when starting up
the AssayMAP Bravo Platform.
The error messages are:
•“Unable to communicate with the device” on page 2
•“There appears to be a plate present” on page 4
•“Verify that it is safe to home the W-axis” on page 5
•“Accessory startup errors” on page 6
If you cannot resolve an error message using the following procedures, see
“Reporting problems” on page viii.
IMPORTANT
Always use the System Startup utility when you first start up the AssayMAP Bravo
Platform and before you run an application or utility after running the System
Shutdown protocol.

2 Common startup error messages
Unable to communicate with the device
2AssayMAP Bravo Platform Error Recovery Guide
Unable to communicate with the device
Startup error message
The Unable to communicate with the device message or similar message may appear
when you attempt to initialize the AssayMAP Bravo Platform.
Resolving the error
Possible causes Recommended actions
The Bravo device is not
turned on.
1If the AssayMAP Bravo is not turned on (indicator light in not on), turn on
the AssayMAP Bravo power switch and turn on all the accessories.
Note: If the AssayMAP Bravo device does not turn on, see the
troubleshooting section of the Bravo Platform User Guide.
2Click Retry.
The communication cable is
damaged or not connected
properly.
1Verify that the Ethernet cable is in good condition. If necessary, reconnect
the Ethernet cable at the computer and the AssayMAP Bravo device.
2At the computer connection, check that the link light next to the Ethernet
cable connector is lit green, and then click Retry.
The LAN connection is
interrupted.
1Click Abort. Close the Protein Sample Prep Workbench and the VWorks
software.
2Power cycle the AssayMAP Bravo device by turning it off and turning it on
again.
3Open the Protein Sample Prep Workbench and run the System Startup
utility again.

3
2 Common startup error messages
Unable to communicate with the device
AssayMAP Bravo Platform Error Recovery Guide
IMPORTANT
If you are connecting to a LAN other than the AssayMAP Bravo Platform, make sure
that the computer has a second LAN card. One LAN card connects to the Bravo. The
second LAN card can connect to a different LAN, such as your company network. Make
sure you verify the computer LAN connection to the AssayMAP Bravo Platform before
connecting any other networks. For details, see the Bravo Platform Safety and
Installation Guide.
Unknown cause. 1Power cycle the AssayMAP Bravo device and accessories, and restart the
computer.
2Open the Protein Sample Prep Workbench and run the System Startup
utility again.
Possible causes Recommended actions

2 Common startup error messages
There appears to be a plate present
4AssayMAP Bravo Platform Error Recovery Guide
There appears to be a plate present
Startup error message
The There appears to be a plate present error message appears during initialization of
the AssayMAP Bravo Platform.
Resolving the error
Cause Recommended action
The first time that the AssayMAP Bravo device
is initialized after turning on the instrument, this
error occurs as a prompt to make sure the
gripper is not holding a plate.
1Make sure that no labware is being held by the
AssayMAP Bravo gripper.
Note: If the gripper is holding labware, open Bravo
Diagnostics, and use the controls in the Gripper tab
to open the gripper and remove the labware.
2Click Ignore and Continue, leaving device in current state
to continue the homing process.

5
2 Common startup error messages
Verify that it is safe to home the W-axis
AssayMAP Bravo Platform Error Recovery Guide
Verify that it is safe to home the W-axis
Startup error message
The Please verify that it is safe to home the W-axis message appears during initialization
of the AssayMAP Bravo Platform.
Resolving the error
IMPORTANT
If you are not using the System Startup utility and this message appears, you should
typically click Retry to continue homing the w-axis.
Cause Recommended action
The first time that the AssayMAP Bravo device
is initialized after turning on the instrument, this
error occurs as a warning so you can manage
any liquid that might be present in the syringes.
For example, if the System Shutdown utility
was used when shutting down the AssayMAP
Bravo, 200 µL of liquid is in each syringe.
If this message appears during a run of the System Startup
utility, click Ignore and Continue, leaving device in current
state.
The Startup utility will automatically home the w-axis in a
later step when the head is moved above the wash station
to ensure that any liquid in the syringes are managed
properly.

2 Common startup error messages
Accessory startup errors
6AssayMAP Bravo Platform Error Recovery Guide
Accessory startup errors
Startup error message examples
The following are just a few examples of messages that can occur if an accessory is
not powered on or if an accessory power or communication cable is disconnected or
loose. The wording of the message varies depending on the accessory and the
problem.
Resolving the error
Cause Recommended action
The accessory is not powered on. 1Ensure that the power cable is in good condition and
properly connected.
2Turn off and turn on the accessory.
3Click Retry.
The accessory communication cable is
damaged or the connections are loose.
1Verify that the communication cable is in good
condition and securely connected.
2Click Retry.

7
3 Common AssayMAP Bravo error
messages
This chapter describes error messages that you may encounter when using the
AssayMAP Bravo Platform.
The error messages are:
•“Could not dock gripper and other communication errors” on page 8
•“Robot disable is active messages” on page 10
•“W-axis was commanded to an invalid destination ” on page 13
If you cannot resolve an error message using the following procedures, see
“Reporting problems” on page viii.

3 Common AssayMAP Bravo error messages
Could not dock gripper and other communication errors
8AssayMAP Bravo Platform Error Recovery Guide
Could not dock gripper and other communication errors
Error message examples
In addition to the errors shown in these examples, some of the other potential
communication and homing errors are:
•Could not home the X-axis or Y-axis.
•Could not align X-axis or Y-axis motor.
•Error in moving to position on X-axis or Y-axis.
•Error homing the X-axis or Y-axis: Axis timed out during commutation.
•No response received from the pipette controller.
•Error retrieving homed status of X-axis or Y-axis while preparing a move.
The exact wording of the error message can vary depending on the Bravo model (for
example, G5563A or G5523A) and the operation that the Bravo device was performing
when the error occurred.
This type of error can occur if you attempt to run an AssayMAP application or utility
after power cycling the Bravo Platform without first exiting the Protein Sample Prep
Workbench and the VWorks software.
A communication can also occur if someone turns off the power to the AssayMAP
Bravo Platform or a power or communication cable is disconnected or loose.
Use the following procedure to resolve any of these types of errors.

9
3 Common AssayMAP Bravo error messages
Could not dock gripper and other communication errors
AssayMAP Bravo Platform Error Recovery Guide
Resolving a communication error
IMPORTANT
Make sure that you run the System Startup utility when starting up the AssayMAP
Bravo Platform.
To resolve a communication error:
1In the AssayMAP Bravo Error dialog box, click Abort.
2In the Scheduler Paused dialog box that opens, click Abort process.
3Exit the Protein Sample Prep Workbench and the VWorks software.
4Power cycle the device as follows:
aTurn off the AssayMAP Bravo Platform and accessories.
bWait a minute, and then turn on the AssayMAP Bravo Platform and
accessories.

3 Common AssayMAP Bravo error messages
Robot disable is active messages
10 AssayMAP Bravo Platform Error Recovery Guide
5Start the Protein Sample Prep Workbench, and open the Utility Library.
6In the Utility Library, locate the System Startup/Shutdown utility, and then click Utility.
Follow the instructions for running the Startup protocol.
Robot disable is active messages
Error message examples
Note: The wording in a given robot disable is active error message may not match the
examples included here.
A robot disable is active error message opens anytime you press the red button on the
emergency-stop pendant or interrupt the Light Curtain, resulting in an emergency stop.
The actual wording in the error message depends on the operation that was in progress
at the time the interlock circuit was tripped.

11
3 Common AssayMAP Bravo error messages
Robot disable is active messages
AssayMAP Bravo Platform Error Recovery Guide
Figure AssayMAP Bravo Platform emergency-stop pendant and Light Curtain
About emergency stops and the safety interlock circuit
IMPORTANT
An emergency stop might be necessary to prevent a collision of the Bravo head with
another object, such as misaligned labware. If you want to temporarily pause and then
continue a run, see “Pausing and continuing a run in the workbench” on page 23.
The AssayMAP Bravo Platform has a safety interlock circuit that is designed to protect
operators from moving-parts hazards. The safety interlock circuit must be closed for
the AssayMAP Bravo Platform to operate. The pendant and the Light Curtain are
connected to the interlock circuit. Pressing the red button on the pendant or
interrupting the Light Curtain trips the interlock circuit, causing the motion of the Bravo
head to stop immediately.
Stopping in an emergency
To stop in an emergency:
Press the red button on the emergency-stop pendant or interrupt the Light Curtain. The
Bravo head stops immediately.
Figure Emergency-stop pendant
The robot disable is active error message opens.
Bravo
B
r
a
v
o

3 Common AssayMAP Bravo error messages
Robot disable is active messages
12 AssayMAP Bravo Platform Error Recovery Guide
Restoring the Bravo device after an emergency stop
To restore the Bravo device after an emergency stop:
1At the emergency-stop pendant, turn the red button clockwise. The spring-loaded
button pops up.
2If applicable. Remove any object that is interrupting the Light Curtain.
3In the AssayMAP Bravo Error dialog box, click Retry.
In most cases, the AssayMAP Bravo Platform will be able to resume the run where
it left off.
Note: In some rare cases, the W-Axis commanded to invalid destination error message
may appear after attempting to retry. If this happens, see the “W-axis was commanded
to an invalid destination ” on page 13 for instructions on how to recover.