Agilent Technologies Cary 3500 User manual

Agilent Cary 3500 UV-Vis Spectrophotometer
User’s Guide

2 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Notices
Manual Part Number
5994-0319EN
Edition 2, December 2018
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© Agilent Technologies, Inc. 2018
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Safety Notices
A CAUTION notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in personal injury or death.
Do not proceed beyond a WARNING
notice until the indicated conditions are
fully understood and met.
WARNING
CAUTION

Contents
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 3
Contents
1General Information and Safety Practices and Hazards 5
User Documentation 5
Conventions 6
Notes and Tips 6
Verifying Safe State 6
Lamp Module 7
Modules, Covers and Panels 7
Compressed gas cylinders 7
Other Precautions 8
Warning and Other Symbols 8
CE Compliance 10
Electromagnetic Compatibility 10
EN55011/CISPR11 10
ICES/NMB-001 11
2Specifications 13
Site Preparation Checklist 13
Measurement category 13
Pollution degree 13
Environmental conditions 14
Weights and dimensions 14
Electrical specifications 15
Mains Supply 15
Computer requirements 16

Contents
4 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Gas requirements 16
3Installation 17
4Introduction 19
Modular concept 19
Modules 19
Cary Multicell Ambient and Peltier 20
Compact Ambient and Peltier 20
Configurations 21
Temperature probes 21
Instrument overview 21
Front and right side of the module and engine 22
Back of the engine 22
Front of the engine 22
Back of the module 23
Indicator LED 23
Using the software 23
Compressed gas purging 24
Purge kit 24
Drain outlet 25
5Troubleshooting, Maintenance and Spare Parts 27
Fuses 27
Spills 28
Spare Parts 28

General Information and Safety Practices and Hazards
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 5
1General Information and Safety Practices
and Hazards
User Documentation 5
Verifying Safe State 6
Lamp Module 7
Modules, Covers and Panels 7
Compressed gas cylinders 7
Other Precautions 8
Warning and Other Symbols 8
CE Compliance 10
Electromagnetic Compatibility 10
Your Agilent instrument and accessories have been carefully designed so that
when used properly you have an accurate, fast, flexible and safe analytical system.
Information about safety practices appears throughout the documentation (both
hard copy and online) provided with your instrument and accessories to help you
safely operate the instrument and accessories. Before using the instrument or
accessories, you must thoroughly read these safety practices. ALWAYS operate
the instrument and accessories in accordance with these safety practices.
User Documentation
You have been provided with the following additional documentation to help you
set up and operate your Agilent Cary 3500 UV-Vis spectrophotometer:
Cary UV software installation instruction sheet found in the software box
Cary 3500 UV-Vis module instruction sheet found in the module box
Cary 3500 UV-Vis engine instruction sheet found in the engine box

General Information and Safety Practices and Hazards
6 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
This manual, with safety practices and hazards information, instructions for
installing and maintaining the components of the Cary 3500 UV-Vis and
troubleshooting information.
The Cary UV Workstation Help and Learning Center provided with the Cary UV
Workstation software which provide step-by-step instructions for installing
selected accessories and programming applications, step-by-step instructions
for frequently performed analyses and instructions for using any accessories
you ordered.
Conventions
The following conventions have been used throughout the documentation:
Menus, menu items, buttons and check boxes have been typed in bold. For
example, ‘click OK’ and ‘From the Edit menu, choose Copy’.
ALL CAPITALS indicate keyboard commands. For example, ‘Press ENTER’ and
‘Press SHIFT+F3’.
Notes and Tips
A Note is used to give advice or information.
A Tip is used to give practical hints to help you achieve the best possible
performance from your instrument.
Verifying Safe State
The following general safety precautions must be observed during all phases of
operation, maintenance and service of this instrument.
To ensure continued safety of the instrument after maintenance or service
procedures verify the instrument is returned to a safe state for the user. This
includes running performance checks to verify the instruments safety systems are
functioning correctly. Check the general condition of the instrument during
operation for wear or signs of corrosion that are likely to inhibit function or safety.
Failure to comply with these precautions or with specific warnings elsewhere in
this manual violates safety standards of design, manufacture, and intended use of
the instrument. Agilent Technologies assumes no liability for the customer’s
failure to comply with these requirements.

General Information and Safety Practices and Hazards
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 7
Lamp Module
The lamp is enclosed in a self-contained module. This module contains
components operating at high voltages. To avoid electric shock, NEVER
disassemble the module.
Modules, Covers and Panels
The only routinely accessed panel is the Cary UV spectrophotometer sample
compartment to introduce samples.
The only other cover and panel that is customer accessible is the lamp module
and is to be removed ONLY when changing the lamp module. Consult the Cary UV
Help and Learning Center for instructions and safety information.
Any other panels or covers that are retained by screws on the spectrophotometer
and accessories may be opened ONLY by Agilent service engineers.
Compressed gas cylinders
Compressed gas cylinders contain highly pressurized gas. If storage conditions
are outside of the recommended supplier’s safety codes, the cylinders can explode
or rapidly release gas into the environment. This may result in injury or death.
Store and handle compressed gases carefully and in strict adherence to safety
codes.
Secure cylinders to an immovable structure or wall.
Store and move cylinders in an upright, vertical position. Before transport,
remove regulators and install cylinder cap.
Store cylinders in a well-ventilated area away from heat, direct sunshine,
freezing temperatures, and ignition sources.
Clearly mark cylinders so there is no doubt as to their contents.
Use only approved regulators and connections.
Use only connector tubing that is chromatographically clean and has a
pressure rating significantly greater than the highest outlet pressure from the
regulator.

General Information and Safety Practices and Hazards
8 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Other Precautions
Use of the Cary UV-Vis system and accessories may involve materials, solvents
and solutions that are flammable, corrosive, toxic or otherwise hazardous.
Careless, improper, or unskilled use of such materials, solvents and solutions can
create explosion hazards, fire hazards, toxicity and other hazards which can result
in death, serious personal injury, and damage to equipment and property.
ALWAYS ensure that laboratory safety practices governing the use, handling and
disposal of such materials are strictly observed. These safety practices should
include the wearing of appropriate safety clothing and safety glasses.
Warning and Other Symbols
The following is a list of symbols that appear in conjunction with warnings on the
spectrophotometer. The hazard they describe is also shown.
A triangular symbol indicates a warning. The meanings of the symbols that may
appear alongside warnings in the documentation or on the instrument itself are as
follows.
Disconnect power
Electrical shock
Heavy weight
(danger to feet)
Heavy weight
(danger to hands)
Hot surface

General Information and Safety Practices and Hazards
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 9
The following symbol may be used on warning labels attached to the instrument.
When you see this symbol, refer to the relevant operation or service manual for the
correct procedure referred to by that warning label.
The following symbols appear on the instrument or accessories:
I
Mains power on
0
Mains power off
Fuse
Single phase alternating current
Protective earth ground terminal
Earth ground terminal
Frame or chassis ground terminal
Nitrogen purge input at the specified flow rate
Caution, disconnect all supplies, risk of electric shock

General Information and Safety Practices and Hazards
10 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
CE Compliance
Your Agilent Cary UV spectrophotometer has been designed to comply with the
requirements of the Electromagnetic Compatibility (EMC) Directive and the Low
Voltage (electrical safety) Directive (commonly referred to as the LVD) of the
European Union. Agilent has confirmed that each product complies with the
relevant Directives by testing a prototype against the prescribed EN (European
Norm) standards.
Proof that a product complies with these directives is indicated by:
the CE Marking appearing on the rear of the product, and
the documentation package that accompanies the product containing a copy
of the Declaration of Conformity. The Declaration of Conformity is the legal
declaration by Agilent that the product complies with the directives listed
above, and shows the EN standards to which the product was tested to
demonstrate compliance.
Electromagnetic Compatibility
EN55011/CISPR11
Group 1 ISM equipment: group 1 contains all Industrial, Scientific and Medical
(ISM) equipment in which there is intentionally generated and/or used
conductively coupled radio- frequency energy which is necessary for the internal
functioning of the equipment itself.
Class A equipment is equipment suitable for use in all establishments other than
domestic and those directly connected to a low voltage power supply network
which supplies buildings used for domestic purposes.
This device complies with the requirements of CISPR11, Group 1, Class A as
radiation professional equipment. Therefore, there may be potential difficulties in
ensuring electromagnetic compatibility in other environments, due to conducted
as well as radiated disturbances.
Operation is subject to the following two conditions:
1This device may not cause harmful interference.
2This device must accept any interference received, including interference that
may cause undesired operation.

General Information and Safety Practices and Hazards
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 11
If this equipment does cause harmful interference to radio or television reception,
which can be determined by turning the equipment off and on, the user is
encouraged to try one or more of the following measures:
1Relocate the radio or antenna.
2Move the device away from the radio or television.
3Plug the device into a different electrical outlet, so that the device and the
radio or television are on separate electrical circuits.
4Make sure that all peripheral devices are also certified.
5Make sure that appropriate cables are used to connect the device to peripheral
equipment.
6Consult your equipment dealer, Agilent Technologies, or an experienced
technician for assistance.
Changes or modifications not expressly approved by Agilent Technologies could
void the user’s authority to operate the equipment.
ICES/NMB-001
This ISM device complies with Canadian ICES-001.
Cet appareil ISM est conforme à la norme NMB-001 du Canada.

General Information and Safety Practices and Hazards
12 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
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Specifications
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 13
2Specifications
Site Preparation Checklist 13
Measurement category 13
Pollution degree 13
Environmental conditions 14
Weights and dimensions 14
Electrical specifications 15
Computer requirements 16
Gas requirements 16
Site Preparation Checklist
Upon purchasing your Cary 3500 UV-Vis system, you were sent the Cary 3500
UV-Vis System Site Preparation Checklist. You must have prepared your site
according to the requirements listed in the checklist before installing and using the
instrument. To obtain another copy of the site preparation checklist, go to the
Agilent website at www.agilent.com and search for ‘Cary 3500 Site Preparation
Checklist’.
Measurement category
The Measurement category is IEC61010:I. Do not to use this equipment for
measurements within measurement categories II, III and IV.
Pollution degree
The pollution degree is IEC61010:2. Pollution degree ‘2’ applies to a normal indoor
atmosphere.

Specifications
14 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Environmental conditions
For detailed environmental conditions, see the Site Preparation Checklist.
The area should have no drafts, no corrosive fumes and no vibrations.
NOTE
For
optimum analytical performance
, it is recommended that the ambient
temperature of the laboratory be between
20 and 25 °C
and be held constant to
within ±2 °C throughout the entire working day.
The area should have a dust-free, low humidity atmosphere. Air conditioning is
recommended. The room should be temperature-controlled if your analyses are
particularly sensitive.
Weights and dimensions
WARNING
Danger to hands and feet
Heavy weight. Always use two people to lift the Cary 3500 UV-Vis engine
and module.
For detailed weight information, see the Site Preparation Checklist.
The workbench should be about 90 cm (36 in) high. Remember to provide space
for the computer, monitor and printer.
To avoid damage through spillage of solutions and samples being analyzed, the
worktops should be covered with a material that is corrosion resistant and
impervious to liquids.
Do not block any ventilation grills present on the computer. Consult the manuals
supplied with your PC, monitor and printer/plotter for their specific ventilation
requirements.
For models with Peltier control you must allow for the circulation of air for cooling.
Allow for 20 cm (8 inches) of space on both sides and approximately 10 cm
(4 inches) in the rear for electrical, gas connections and air flow.

Specifications
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 15
For models that have Peltier temperature control, clear and unobstructed airflow
of the inlet and outlet must be maintained. Ambient air must be allowed to enter
the inlet and other heat sources such as exhausts, computers or other nearby
Peltier modules must be avoided.
For models without Peltier control, you must allow for the circulation of air for
cooling. Allow for 10 cm (4 inches) of space on both sides and approximately
10 cm (4 inches) in the rear for electrical connections and air flow.
Electrical specifications
For detailed power requirements see the Site Preparation Checklist.
The power cord and all other connections are located at the rear of the instrument
or on the side of the module. The power switch is located on the rear of the engine
and on the side panel of the module. Position the instrument for easy access to
the disconnecting device.
Mains Supply
Power cords are provided based on the user’s country requirements. Only the
supplied power cord is to be used with this equipment.
The installation of electrical power supplies must comply with the rules and/or
regulations imposed by local authorities responsible for the supply of electrical
energy to the workplace.
WARNING
Shock Hazard
Danger of electrocution. Good electrical grounding is essential to avoid
potentially serious shock hazards. A 3-wire outlet with ground connection
must be provided for the Cary 3500 UV-Vis. Make certain that power outlets
are earth-grounded at the grounding pin.
For safety reasons, a separate power outlet receptacle should be provided for
each unit in the system. The use of extension cords or outlet adaptors is not
recommended.
If necessary, replace the power cord only with a cord equivalent to the one
specified in the Site Preparation Checklist.

Specifications
16 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Computer requirements
Cary UV systems are evaluated under Agilent’s ISO9001 procedures using Cary UV
Workstation software and a desktop computer. We strongly recommend a
computer configuration that matches the system that was used during
evaluations.
Table 1 Computer requirements
Component
Requirement
Processor
3.3 GHz, 8MB cache
Memory
8 GB RAM
Storage
500 GB SATA 7200 RPM HDD
DVD drive
16x DVD+ -RW
Graphics
Intel® HD Graphics P530
Communications
PCIe GbE Controller; 2nd Serial Port Adapter, 2nd LAN NIC
Operating system
Microsoft Windows 10 64-bit
Locate the PC keyboard and mouse for ergonomically correct access.
Gas requirements
The Cary 3500 UV-Vis requires the supply of a compressed gas (nitrogen or air)
when the Peltier temperature-controlled modules are used. It is required to prevent
condensation of the sample compartment when temperatures below ambient are
used. The supplied gas must be dry, oil and particle free and non-combustible.
Table 2 Purge gas purity requirements
Compressed gas
Description
Nitrogen
Water vapor <2570 ppm (dew point @ -10 °C). Acceptable sources are from
bottled gas with industrial, medical or food grade nitrogen.
Air
Compressed Air: ISO 8573-1:2010 CLASS 1.3.1

Installation
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 17
3Installation
Use the following checklist to make sure you have the work area ready to install
the Cary 3500 UV-Vis.
Preparation requirement
Complete
All equipment is on site and has been checked for damage.

The work area meets the environmental requirements (see Page 14).

A suitable workbench is available (see Page 14).

Suitable electrical power supplies are available (see Page 15).

A computer that meets requirements is available (see Page 16).

A working Microsoft Windows operating system is installed on the
computer. For instructions on installing this, refer to the documentation
supplied with the operating system.

The Agilent Cary 3500 UV-Vis spectrophotometer is designed to be completely
customer-installable. Instructions for setting up the system are included in the
Cary UV-Vis Installation Video that is installed on the computer desktop during the
software installation.
There are three main steps to install your Cary UV system:
1Install the software – instructions are provided in the Cary UV software box
2Install the hardware including both the engine and module – instructions are
provided in the installation video installed on your computer desktop
3Run the System Heath tests to ensure the installation was successful –
instructions are provided in the installation video installed on your computer
desktop and in the Cary UV-Vis Help and Learning Center
Once the installation is complete, open the Cary UV-Vis Help and Learning Center.
Click “Learning” to access a self-guided familiarization of the hardware and
software, including instructions on how to setup and run a method and how to
analyze the data.

Installation
18 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
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Introduction
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 19
4Introduction
Modular concept 19
Modules 19
Configurations 21
Temperature probes 21
Instrument overview 21
Indicator LED 23
Using the software 23
Compressed gas purging 24
Drain outlet 25
_Toc506284002Modular concept
The Cary 3500 UV-Vis introduces a modular concept to the Agilent Cary range of
high performance spectrophotometers. Modularity splits instrument function
between light generation and light measurement. The Cary 3500 UV-Vis engine
produces monochromatic light which is measured by the various Cary 3500 UV-Vis
sample measurement modules. This allows for the tailored development of specific
modules for applications while maximizing the flexibility of the system.
The Cary engine is based around Xenon flash lamp technology coupled with a
compact dual out of plane monochromator. As with any spectrophotometer
photometric performance is largely determined by the quality of the
monochromator. With UV-Vis instruments, the monochromator design is aimed at
providing a desired wavelength resolution while maintaining high light throughput
and low stray light.
Modules
Cary 3500 UV-Vis modules couple with the engine to provide measurement
functionality for target applications. The Multicell and Compact UV-Vis modules
provide measurement solutions for cuvette-based applications.

Introduction
20 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Both modules employ fiber optic technology to split and distribute light from the
engine to each of their measurement channels.
The Compact UV-Vis module has 2 measurement channels: sample and
reference/blank. The Multicell module provides 8 measurement channels. Both
modules come as ambient or Peltier variants.
Cary Multicell Ambient and Peltier
This module provides thermal control of the 8 cuvette stations. Control is arranged
in 4 zones each containing one channel pair (positions 1/2, 3/4, etc.).
For the Peltier version, the temperature of each zone can be varied over a range of
0 to 110 °C and can be controlled based on either the temperature of the cell holder
(block) or the temperature of the sample by using the sample temperature probe
accessory. The Multizone feature allows for individual control of each zone.
WARNING
Hot surface
The sample compartment and cuvettes may become extremely hot during
instrument operation and remain hot for some time after the temperature
has reduced. Allow the sample compartment and cuvettes to cool for at
least five minutes before attempting to remove cuvettes.
NOTE
Laboratory conditions directly affect temperature control. Because this is an air-
cooled system, when working at the lower temperature ranges, the laboratory
conditions must meet the lower end of the Environmental Specifications.
Compact Ambient and Peltier
This module provides thermal control of the two cuvette positions.
The Peltier version provides similar thermal control as Multicell Peltier in one
channel pair (zone). Control may be based on either the cell holder (block) or the
actual sample temperature by using the sample temperature probe accessory.