Agilent Technologies Cary 3500 User manual

Agilent Cary 3500 UV-Vis Spectrophotometer
User’s Guide

2 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Notices
Manual Part Number
5994-0319EN
Edition 2, December 2018
Copyright
© Agilent Technologies, Inc. 2018
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Safety Notices
A CAUTION notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in personal injury or death.
Do not proceed beyond a WARNING
notice until the indicated conditions are
fully understood and met.
WARNING
CAUTION

Contents
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 3
Contents
1General Information and Safety Practices and Hazards 5
User Documentation 5
Conventions 6
Notes and Tips 6
Verifying Safe State 6
Lamp Module 7
Modules, Covers and Panels 7
Compressed gas cylinders 7
Other Precautions 8
Warning and Other Symbols 8
CE Compliance 10
Electromagnetic Compatibility 10
EN55011/CISPR11 10
ICES/NMB-001 11
2Specifications 13
Site Preparation Checklist 13
Measurement category 13
Pollution degree 13
Environmental conditions 14
Weights and dimensions 14
Electrical specifications 15
Mains Supply 15
Computer requirements 16

Contents
4 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Gas requirements 16
3Installation 17
4Introduction 19
Modular concept 19
Modules 19
Cary Multicell Ambient and Peltier 20
Compact Ambient and Peltier 20
Configurations 21
Temperature probes 21
Instrument overview 21
Front and right side of the module and engine 22
Back of the engine 22
Front of the engine 22
Back of the module 23
Indicator LED 23
Using the software 23
Compressed gas purging 24
Purge kit 24
Drain outlet 25
5Troubleshooting, Maintenance and Spare Parts 27
Fuses 27
Spills 28
Spare Parts 28

General Information and Safety Practices and Hazards
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 5
1General Information and Safety Practices
and Hazards
User Documentation 5
Verifying Safe State 6
Lamp Module 7
Modules, Covers and Panels 7
Compressed gas cylinders 7
Other Precautions 8
Warning and Other Symbols 8
CE Compliance 10
Electromagnetic Compatibility 10
Your Agilent instrument and accessories have been carefully designed so that
when used properly you have an accurate, fast, flexible and safe analytical system.
Information about safety practices appears throughout the documentation (both
hard copy and online) provided with your instrument and accessories to help you
safely operate the instrument and accessories. Before using the instrument or
accessories, you must thoroughly read these safety practices. ALWAYS operate
the instrument and accessories in accordance with these safety practices.
User Documentation
You have been provided with the following additional documentation to help you
set up and operate your Agilent Cary 3500 UV-Vis spectrophotometer:
Cary UV software installation instruction sheet found in the software box
Cary 3500 UV-Vis module instruction sheet found in the module box
Cary 3500 UV-Vis engine instruction sheet found in the engine box

General Information and Safety Practices and Hazards
6 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
This manual, with safety practices and hazards information, instructions for
installing and maintaining the components of the Cary 3500 UV-Vis and
troubleshooting information.
The Cary UV Workstation Help and Learning Center provided with the Cary UV
Workstation software which provide step-by-step instructions for installing
selected accessories and programming applications, step-by-step instructions
for frequently performed analyses and instructions for using any accessories
you ordered.
Conventions
The following conventions have been used throughout the documentation:
Menus, menu items, buttons and check boxes have been typed in bold. For
example, ‘click OK’ and ‘From the Edit menu, choose Copy’.
ALL CAPITALS indicate keyboard commands. For example, ‘Press ENTER’ and
‘Press SHIFT+F3’.
Notes and Tips
A Note is used to give advice or information.
A Tip is used to give practical hints to help you achieve the best possible
performance from your instrument.
Verifying Safe State
The following general safety precautions must be observed during all phases of
operation, maintenance and service of this instrument.
To ensure continued safety of the instrument after maintenance or service
procedures verify the instrument is returned to a safe state for the user. This
includes running performance checks to verify the instruments safety systems are
functioning correctly. Check the general condition of the instrument during
operation for wear or signs of corrosion that are likely to inhibit function or safety.
Failure to comply with these precautions or with specific warnings elsewhere in
this manual violates safety standards of design, manufacture, and intended use of
the instrument. Agilent Technologies assumes no liability for the customer’s
failure to comply with these requirements.

General Information and Safety Practices and Hazards
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 7
Lamp Module
The lamp is enclosed in a self-contained module. This module contains
components operating at high voltages. To avoid electric shock, NEVER
disassemble the module.
Modules, Covers and Panels
The only routinely accessed panel is the Cary UV spectrophotometer sample
compartment to introduce samples.
The only other cover and panel that is customer accessible is the lamp module
and is to be removed ONLY when changing the lamp module. Consult the Cary UV
Help and Learning Center for instructions and safety information.
Any other panels or covers that are retained by screws on the spectrophotometer
and accessories may be opened ONLY by Agilent service engineers.
Compressed gas cylinders
Compressed gas cylinders contain highly pressurized gas. If storage conditions
are outside of the recommended supplier’s safety codes, the cylinders can explode
or rapidly release gas into the environment. This may result in injury or death.
Store and handle compressed gases carefully and in strict adherence to safety
codes.
Secure cylinders to an immovable structure or wall.
Store and move cylinders in an upright, vertical position. Before transport,
remove regulators and install cylinder cap.
Store cylinders in a well-ventilated area away from heat, direct sunshine,
freezing temperatures, and ignition sources.
Clearly mark cylinders so there is no doubt as to their contents.
Use only approved regulators and connections.
Use only connector tubing that is chromatographically clean and has a
pressure rating significantly greater than the highest outlet pressure from the
regulator.

General Information and Safety Practices and Hazards
8 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
Other Precautions
Use of the Cary UV-Vis system and accessories may involve materials, solvents
and solutions that are flammable, corrosive, toxic or otherwise hazardous.
Careless, improper, or unskilled use of such materials, solvents and solutions can
create explosion hazards, fire hazards, toxicity and other hazards which can result
in death, serious personal injury, and damage to equipment and property.
ALWAYS ensure that laboratory safety practices governing the use, handling and
disposal of such materials are strictly observed. These safety practices should
include the wearing of appropriate safety clothing and safety glasses.
Warning and Other Symbols
The following is a list of symbols that appear in conjunction with warnings on the
spectrophotometer. The hazard they describe is also shown.
A triangular symbol indicates a warning. The meanings of the symbols that may
appear alongside warnings in the documentation or on the instrument itself are as
follows.
Disconnect power
Electrical shock
Heavy weight
(danger to feet)
Heavy weight
(danger to hands)
Hot surface

General Information and Safety Practices and Hazards
Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 9
The following symbol may be used on warning labels attached to the instrument.
When you see this symbol, refer to the relevant operation or service manual for the
correct procedure referred to by that warning label.
The following symbols appear on the instrument or accessories:
I
Mains power on
0
Mains power off
Fuse
Single phase alternating current
Protective earth ground terminal
Earth ground terminal
Frame or chassis ground terminal
Nitrogen purge input at the specified flow rate
Caution, disconnect all supplies, risk of electric shock

General Information and Safety Practices and Hazards
10 Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide
CE Compliance
Your Agilent Cary UV spectrophotometer has been designed to comply with the
requirements of the Electromagnetic Compatibility (EMC) Directive and the Low
Voltage (electrical safety) Directive (commonly referred to as the LVD) of the
European Union. Agilent has confirmed that each product complies with the
relevant Directives by testing a prototype against the prescribed EN (European
Norm) standards.
Proof that a product complies with these directives is indicated by:
the CE Marking appearing on the rear of the product, and
the documentation package that accompanies the product containing a copy
of the Declaration of Conformity. The Declaration of Conformity is the legal
declaration by Agilent that the product complies with the directives listed
above, and shows the EN standards to which the product was tested to
demonstrate compliance.
Electromagnetic Compatibility
EN55011/CISPR11
Group 1 ISM equipment: group 1 contains all Industrial, Scientific and Medical
(ISM) equipment in which there is intentionally generated and/or used
conductively coupled radio- frequency energy which is necessary for the internal
functioning of the equipment itself.
Class A equipment is equipment suitable for use in all establishments other than
domestic and those directly connected to a low voltage power supply network
which supplies buildings used for domestic purposes.
This device complies with the requirements of CISPR11, Group 1, Class A as
radiation professional equipment. Therefore, there may be potential difficulties in
ensuring electromagnetic compatibility in other environments, due to conducted
as well as radiated disturbances.
Operation is subject to the following two conditions:
1This device may not cause harmful interference.
2This device must accept any interference received, including interference that
may cause undesired operation.