Agilent Technologies 7200 User manual

Agilent Technologies
Agilent
7200 Accurate-Mass
Quadrupole
Time-of-Flight GC/MS
System
Operation Manual

Notices
© Agilent Technologies, Inc. 2012, 2014
No part of this manual may be reproduced in
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tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G3850-90008
Edition
First Edition, August 2014
Printed in USA
Agilent Technologies, Inc.
5301 Stevens Creek Blvd.
Santa Clara, CA 95051
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A WARNING notice denotes a
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tions are fully understood and
met.

7200 Accurate-Mass QTOF Operation Manual 3
About This Manual
This manual contains information for operating and maintaining the Agilent
7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS system.
1“Introduction”
Chapter 1 describes general information about the 7200 Q-TOF GC/MS,
including a hardware description, and general safety warnings.
2“Installing GC Columns”
Chapter 2 shows you how to prepare a capillary column for use with the MS,
install it in the GC oven, and connect it to the MS using the GC/MS interface.
3“Operating in Electron Impact (EI) Mode”
Chapter 3 describes routine operations in EI mode such as setting
temperatures, monitoring pressures, tuning, venting, and pumpdown.
4“Operating in Chemical Ionization (CI) Mode”
Chapter 4 describes additional tasks necessary to operate in CI mode.
5“Using the Removable Ion Source”
Chapter 5 shows you how to change the removable ion source.
6“General Maintenance”
Chapter 6 describes general maintenance procedures for the 7200 Q-TOF
GC/MS.

4 7200 Accurate-Mass QTOF Operation Manual
Hardware User Information
Accompanying your hardware and software is a comprehensive collection of
manuals, videos, user applications, and method development tools. These are
located on the:
To Install Your Hardware Library
Insert Disk 1 into your DVD drive and follow the prompts.
This can be installed by anyone who has authority to copy
information onto the receiving computer.
To Install Your Software Library
Insert the memory stick into a USB port and follow the prompts.
This can be installed by anyone who has authority to copy
information onto the receiving computer.
©2013 Agilent Technologies,Inc.
Allrights reserved.
Madein USA
Agilent Technologies
GC/GCMS Hardware User Manuals and Videos
S1
Disk2/3
Toinstall:
1.Insert disk into DVD drive
2.Follow the instructions on the screen.
3.If autoplay is not enabled, double-click
"index_xx.html"on the DVD
where xx is:
enfor English
chfor中文
jpfor 日本語
VersionB.01.05
January,2013
©2013 Agilent Technologies,Inc.
Allrights reserved.
Madein USA
Agilent Technologies
GC/GCMS Hardware User Manuals and Videos
S1
Disk2/3
Toinstall:
1.Insert disk into DVD drive
2.Follow the instructions on the screen.
3.If autoplay is not enabled, double-click
"index_xx.html"on the DVD
where xx is:
enfor English
chfor中文
jpfor 日本語
VersionB.01.05
January,2013
•Agilent GC and GC/MS User Manuals and Tools DVD set
•Agilent GC/MS Software Information and Manuals memory stick
See the Agilent 7200 Accurate Mass Q-TOF GC/MS System Quick Start
document (G6845-90014) for more details on how to install this information
on your computer.

7200 Accurate-Mass QTOF Operation Manual 5
Contents
1 Introduction
Abbreviations Used 10
The 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS
System 12
Hardware Description 14
Important Safety Warnings 15
Safety and Regulatory Certifications 18
Intended Use 21
Cleaning/Recycling the Product 21
Liquid Spills 21
Moving or Storing the MS 21
2 Installing GC Columns
Columns 24
To Install a Capillary Column in a Split/Splitless Inlet 26
To Condition a Capillary Column 29
To Install a Capillary Column in the GC/MS Interface 30
3 Operating in Electron Impact (EI) Mode
Operating the MS from the Data System 34
The GC/MS Interface 35
Before You Turn On the MS 37
Pumping Down 38

6 7200 Accurate-Mass QTOF Operation Manual
Controlling Temperatures 38
Controlling Column Flow 38
Controlling Collision Cell Flow 39
Venting the MS 39
Typical Vacuum Pressures in EI Mode 40
To Set Monitors for Temperature and Vacuum Status 41
To Set the MS Analyzer Temperature 43
To Set the GC/MS Interface Temperature from the MassHunter Workstation 45
To Calibrate the Column 46
To Configure the Collision Cell Gas 48
To Set the Collision Cell Gas Flow Rate 49
To Autotune the MS for EI Mode 50
To Remove the Upper RIS Cover 52
To Open the Analyzer Cover for Access to the Analyzer Sideplate 53
To Pump Down the MS 54
To Vent the MS 56
4 Operating in Chemical Ionization (CI) Mode
Setting Up Your MS to Operate in CI Mode 58
The GC/MS Interface 59
Operating the CI MS 61
High Vacuum Pressure in CI Mode 62
Other Reagent Gases 63
CI Autotune 65
The Flow Control Module 66
To Switch from the EI Mode to CI Mode 67

7200 Accurate-Mass QTOF Operation Manual 7
To Operate the Reagent Gas Flow Control Module 69
To Set a Reagent Gas Flow 71
To Perform a CI Autotune 72
5 Using the Removable Ion Source
The Removable Ion Source 76
To Change the Ion Source 78
To Install the RIS Probe Extraction Tool 80
To Remove the Source from the Analyzer Chamber 82
To Change the Ion Source on the RIS Probe Extraction Tool 84
To Install the Ion Source in the Analyzer Chamber 86
To Remove the RIS Probe Extraction Tool from the Instrument 87
6 General Maintenance
Before Starting 90
Maintaining the Vacuum System 94
Maintaining the Analyzer 95
To Disassemble the EI Ion Source 97
To Assemble the EI Ion Source 99
To Disassemble the CI Ion Source 101
To Assemble the CI Ion Source 103
To Clean the Ion Source 105
To Remove a Filament 109
To Install a Filament 111

8 7200 Accurate-Mass QTOF Operation Manual

9
Agilent 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS System
Operation Manual
Agilent Technologies
1
Introduction
Abbreviations Used 10
The 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS System 12
Hardware Description 14
Important Safety Warnings 15
Safety and Regulatory Certifications 18
Intended Use 21
Cleaning/Recycling the Product 21
Liquid Spills 21
Moving or Storing the MS 21
This section provides general information about the 7200 Accurate-Mass
Quadrupole Time-of-Flight (Q-TOF) GC/MS System, including a hardware
description, and general safety warnings.

10 7200 Accurate-Mass QTOF Operation Manual
1Introduction
Abbreviations Used
The abbreviations in Table 1 are used in discussing this product. They are
collected here for convenience.
Table 1 Abbreviations
Abbreviation Definition
AC Alternating current
ALS Automatic liquid sampler
BFB Bromofluorobenzene (calibrant)
CC Collision cell
CI Chemical ionization
DC Direct current
DFTPP Decafluorotriphenylphosphine (calibrant)
EI Electron impact
EPC Electronic pneumatic control
eV Electron volt
GC Gas chromatograph
id Inside diameter
LAN Local Area Network
m/z Mass to charge ratio
MFC Mass flow controller
MS Mass spectrometer
NCI Negative chemical ionization
OFN Octafluoronaphthalene (sample)
PCI Positive chemical ionization
PFDTD Perfluoro-5,8-dimethyl-3,6,9-trioxydodecane (calibrant)
PFET 2,4,6-tris (Pentafluoroethyl)-1,3,5-triazine