Agilent Technologies AssayMAP Bravo User manual


AssayMAP
Bravo Platform
Installation Guide
Original Instructions

Notices
© Agilent Technologies, Inc. 2018
No part of this manual may be reproduced
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electronic storage and retrieval or
translation into a foreign language) without
prior agreement and written consent from
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United States and international copyright
laws.
Installation Guide Part Number
G5571-90001
Edition
Revision B, March 2018
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Automation Solutions
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(November 1995), as applicable in any
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Safety Notices
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed or
adhered to, could result in personal
injury or death. Do not proceed
beyond a WARNING notice until the
indicated conditions are fully
understood and met.
A CAUTION notice denotes a hazard. It
calls attention to an operating procedure,
practice, or the like that, if not correctly
performed or adhered to, could result in
damage to the product or loss of important
data. Do not proceed beyond a CAUTION
notice until the indicated conditions are
fully understood and met.

iiiAssayMAP Bravo Platform Installation Guide
Contents
1. Before you start. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
About this guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Related guides and where to find them. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Safety information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1. Installing the platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Overview of the hardware components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Computer requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Installation workflow for standard configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Installing the Bravo risers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
About installing the AssayMAP head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Installing the 96AM Wash Station and Pump Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Installing the standard platepads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Installing the Peltier Thermal Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Installing and connecting the Orbital Shaking Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
2. Setting up the computer and software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Setting up the computer and installing the software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Turning on the platform and setting up the VWorks software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Verifying the profile settings and configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
About verifying the deck configuration in the profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Verifying the Autofill Station configuration (96AM Wash Station). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Verifying the Peltier Thermal Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Verifying the Orbital Shaking Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Initializing the profile that you are verifying . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Testing the accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
3. Setting the teachpoints . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Teaching part 1: Setting teachpoints in AssayMAP 1 profile. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Teaching part 2: Adjusting teachpoints for accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Teaching part 3: Updating the other profiles based on AssayMAP 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Checking and adjusting the gripper offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
4. Verifying the installation and setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Verification workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
About installing the Bravo pendant, Light Curtain, and shields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Opening the Workbench and accessing the utilities. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Running the Startup utility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Testing the safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Washing the syringes to prepare for first time use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Performing a mock run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Running the Syringe Test utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Running the Shutdown utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

Contents
iv AssayMAP Bravo Platform Installation Guide
Workflow for setting teachpoints in each profile manually. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Adjusting the teachpoint for the Bravo Plate Riser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Adjusting the teachpoint for the red PCR plate insert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

1
AssayMAP Bravo Platform
Installation Guide

1
Before you start
•“About this guide” on page 2
•“Related guides and where to find them” on page 2
•“Service documentation” on page 4
•“Safety information” on page 4

Before you start
About this guide
2AssayMAP Bravo Platform Installation Guide
About this guide
Intent
This installation guide is intended for use by qualified and authorized Agilent
Technologies service personnel who possess an in- depth service background
and have received training in the installation of the AssayMAP Bravo Platform.
What this guide covers
This guide describes the following software versions:
•Protein Sample Prep Workbench v3.0
•VWorks Automation Control v13.1.1
•Bravo Diagnostics v19.1.1
What is new in this revision
and
Related guides and where to find them
Checklists
You can find the following checklists on the Agilent AssayMAP Bravo Platform
product page at https://www.agilent.com/en/products/automation- solutions/
protein- sample- preparation/assaymap- bravo- platform
•Site Preparation Checklist. Specifies the site requirements that must be
met before installing the AssayMAP Bravo Platform.
•Installation Checklist. Lists the tasks to be performed to install the
AssayMAP Bravo Platform.
Feature and description See…
Revised the startup procedure to match
instructions in the Protein Sample Prep
Workbench v3.0.
“Running the Startup utility” on
page 83
Reorganized the workflow for testing the
safety equipment.
“Testing the safety equipment” on
page 84
Revised the syringe wash procedure to
specify 60 wash cycles.
“Washing the syringes to prepare
for first time use” on page 86

3
Before you start
Related guides and where to find them
AssayMAP Bravo Platform Installation Guide
User guides
The following table lists the user guides and where to find them.
To access the VWorks Knowledge Base, do one of the following:
•From within VWorks software, select Help > Knowledge Base or press F1.
•From the Windows desktop, select Start > Agilent Technologies >
VWorks Knowledge Base.
•Go to the online VWorks Knowledge Base at www.agilent.com/chem/askb
and click the link for PDF user guides.
Guide Description Where to find the guide
Bravo Platform Unpacking
Guide
Unpacking instructions for the Bravo
Platform
Attached to shipping
container and in VWorks
Knowledge Base
Bravo Platform Safety and
Installation Guide
Safety guidelines, site specifications,
and installation procedures
Included in the shipping
container and in VWorks
Knowledge Base
Bravo Platform User Guide Setting up, operating, maintaining,
and troubleshooting the Bravo
Platform
VWorks Knowledge Base and
Protein Sample Prep
Workbench
Pump Module User Guide Setting up the Pump Module and the
autofilling reservoirs and wash
stations
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP Bravo Platform
Error Recovery Guide
Error messages, and how to handle
them
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP Bravo Platform
Labware Reference Guide
Labware photo reference and
descriptions
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP Bravo Syringe
Replacement User Guide
Replacing a defective syringe in the
Bravo 96AM Head
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP 96AM Wash
Station Maintenance Guide
Replacing chimneys in the 96AM
Wash Station
VWorks Knowledge Base
VWorks Automation Control
Setup Guide
Defining labware and labware classes,
liquid classes, and pipetting
techniques, and how to track and
manage labware in storage
VWorks Knowledge Base
VWorks Automation Control
User Guide
Creating and editing VWorks
protocols and application interfaces
VWorks Knowledge Base
AssayMAP Syringe Test
Utility User Guide
Running a syringe test of the Bravo
96AM Head
Utility instructions in the
Protein Sample Prep
Workbench

Before you start
Safety information
4AssayMAP Bravo Platform Installation Guide
To access the context-sensitive help feature in the VWorks software:
1In the main window of the VWorks software, click the help button .
The pointer changes to . Notice that the different icons or areas are
highlighted as you move the pointer over them.
2Click an icon or area of interest. The relevant topic or document opens.
Service documentation
Agilent field service engineers can find instructions on how to maintain and
repair the Bravo Platform in the following guides:
•Bravo Platform Service Guide
•Pipette Head Service Guide
Changes to existing procedures and additional service procedures are published
as service bulletins and posted in the Support Portal at
http://intranet.chem.agilent.com/sites/partners/support/default.aspx
Safety information
Before installing and using the Bravo Platform, make sure you are aware of the
potential hazards and understand how to avoid being exposed to them. You
must be properly trained in the correct and safe installation and operation of
the device. For details, see the Bravo Platform Safety and Installation Guide.
Do not substitute parts or make any unauthorized modifications to the Bravo
Platform or the supplied safety equipment.
WARNING Changing or modifying the Bravo Platform safety equipment may prevent
the safe operation of the device, invalidate its safety compliance, and lead to personal
injury or property damage. Any customer who does not use the supplied safety equipment
or who modifies the supplied safety equipment assumes full responsibility for providing an
appropriate level of safety for its operators and for providing the applicable safety
compliance marking and documentation.

1
AssayMAP Bravo Platform
Installation Guide

1
Installing the platform
•“Overview of the hardware components” on page 2
•“Computer requirements” on page 3
•“Installation workflow for standard configuration” on page 4
•“Installing the Bravo risers” on page 5
•“Installing the 96AM Wash Station and Pump Module” on
page 10
•“Installing the standard platepads” on page 20
•“Installing the Peltier Thermal Station” on page 21
•“Installing and connecting the Orbital Shaking Station” on
page 26

Installing the platform
Overview of the hardware components
2AssayMAP Bravo Platform Installation Guide
Overview of the hardware components
The following figure and table show the components of the AssayMAP Bravo
Platform, which is required for running the AssayMAP protocols.
Figure AssayMAP Bravo Platform components
Item Required hardware
1Gripper upgrade
2Bravo 96AM Head
396AM Wash Station
4Pump Module 2.0 and two carboys
596AM Cartridge & Tip Seating Station
6Risers, 146 mm
7Peltier Thermal Station with STC controller
8Custom plate nest
9Red PCR Plate Insert
10 Orbital Shaking Station with Control Unit

3
Installing the platform
Computer requirements
AssayMAP Bravo Platform Installation Guide
In addition to the components listed in the preceding table, the AssayMAP
Bravo Platform includes:
•Bravo Standard Light Curtain and the emergency- stop pendant shown in
the following figures.
•Computer workstation
Figure Standard configuration with Light Curtain
Figure Emergency-stop pendant
Computer requirements
The software user interface for the AssayMAP applications is optimized for a
monitor with a resolution of 1920 x 1080 (full HD). For a full list of the
computer requirements, see the software release notes.
66.5 cm
52.5 cm
84.3 cm

Installing the platform
Installation workflow for standard configuration
4AssayMAP Bravo Platform Installation Guide
Installation workflow for standard configuration
Step For this task... See...
1Verify that the installation site meets the site
requirements.
IMPORTANT Ensure that the laboratory table is not
bowed and is perfectly flat and rigid. A bowed or wobbly
table will cause placement errors during protocol
execution.
Clear the table of all other material that may interfere
with the installation.
Bravo Platform Safety and
Installation Guide
2Unpack and connect the computer. Manufacturer’s instructions
3Unpack the Bravo Platform and accessories.
IMPORTANT Do not remove the y- axis shipping stop
from the head mount until after you have installed the
risers (“Installing the Bravo risers” on page 5).
Bravo Platform Unpacking
Guide
4Install the Bravo deck risers. “Installing the Bravo risers” on
page 5
5Connect the Bravo Platform power cord, communications
cable, and pendant cable.
Bravo Platform Safety and
Installation Guide
6Install the Bravo 96AM Head. “About installing the AssayMAP
head” on page 8
7Install the 96AM Wash Station and Pump Module. “Installing the 96AM Wash
Station and Pump Module” on
page 10
8Install the standard platepads at the specified Bravo
deck locations.
“Installing the standard
platepads” on page 20
9Install the Peltier Thermal Station and the Inheco STC
Controller.
“Installing the Peltier Thermal
Station” on page 21
10 Install the Orbital Shaking Station. “Installing and connecting the
Orbital Shaking Station” on
page 26
11 Set up the software, and configure the device files. “Setting up the computer and
software” on page 31
12 Set the AssayMAP head teachpoints in every one of the
AssayMAP profiles. Verify the gripper offset in every
profile.
“Setting the teachpoints” on
page 57
13 Install the Bravo shields and Light Curtain. Bravo Platform Safety and
Installation Guide
14 Verify the installation and setup. “Verifying the installation and
setup” on page 79

5
Installing the platform
Installing the Bravo risers
AssayMAP Bravo Platform Installation Guide
Installing the Bravo risers
Before you start
IMPORTANT The following procedure is intended only for fully qualified and
trained Agilent service engineers.
Ensure that you have the following:
•Two people to lift the Bravo Platform
•Foam pad (3- inch thick) or similar cushioning material to use when you
tilt the Bravo Platform onto its back plate
Note: EPE foam is preferred due to its firmness.
•Risers
•Hex drivers
Procedure
To install the risers:
1Place the foam pad on the work table behind the Bravo Platform.
WARNING The Bravo device weighs approximately 52.1 kg (114.9 lb). Attempting to
move the Bravo device without assistance could cause personal injury. Request assistance
and use proper lifting techniques when moving the Bravo device.
CAUTION Tugging on the tie bar or using it to lift the Bravo Platform can damage the
device. Do not grab the tie bar when lifting the Bravo Platform.
00233
Bravo
Proper lift
00233
00234
Bravo
Improper lift
00234
CAUTION When tilting the Bravo Platform backwards to rest on its back plate, the head
mount can slide back rapidly along the y-axis and collide with the back plate. To prevent
potential equipment damage, ensure that the y-axis shipping stop remains installed on the
head mount until after you install the risers. If the shipping stop has already been removed
during unpacking, ensure that the head mount is resting against the Bravo back plate before
tilting the Bravo Platform backwards.

Installing the platform
Installing the Bravo risers
6AssayMAP Bravo Platform Installation Guide
2Ensure that the head mount is resting against the Bravo back plate. With a
person positioned on each side of the Bravo Platform, slowly tilt the device
backwards until the back plate rests on the foam.
3Remove the four rubber feet from the base of the Bravo deck.
Figure Bottom view of Bravo deck
4On the bottom of the Bravo deck, install the two risers, one on each side.
Use the four screws and washers provided to install each riser. Tighten the
screws as follows:
aTighten the screws at the top first, and then tighten the screws at the
bottom, as the following figures show.
bTighten the screws until snug, and ensure that the risers are flush to
the base of the Bravo deck.
Figure Top screw located at inner hole

7
Installing the platform
Installing the Bravo risers
AssayMAP Bravo Platform Installation Guide
Figure Bottom screw located at outer hole
5Ensure the risers are installed securely, and the outside edges of the risers
are flush with the Bravo base.
6Lift the Bravo Platform up onto the risers using proper lifting techniques.
Figure Bravo Platform on risers with no deck platepads
Ensure that the instrument and riser assembly does not wobble and that it
sits flat on the table. If the Bravo Platform has any wobble or the risers
have uneven seating, replace or realign and tighten the risers.
WARNING Attempting to lift the Bravo device without assistance could cause
personal injury and result in equipment damage. Request assistance and use proper
lifting techniques when lifting the Bravo device.

Installing the platform
About installing the AssayMAP head
8AssayMAP Bravo Platform Installation Guide
Ensure that you provide 30.48- cm (12- inch) clearance behind the Bravo
Platform.
IMPORTANT Make sure that you remove the y- axis shipping stop from the
Bravo head mount. For instructions, see the Bravo Platform Unpacking Guide.
IMPORTANT Before you install any platepads or accessories, use a clean lint-
free cloth to wipe the Bravo deck and ensure that it is clean.
About installing the AssayMAP head
Before you install the head
The Bravo 96AM Head has four retractable tip- box stripper pins, two on each
side of the probes, as the following figure shows. Before you install the Bravo
96AM Head, ensure that the tip- box stripper pins are retracted, as the
following figure shows.
Figure Bravo 96AM Head (upside down) with stripper pins retracted
If necessary, perform the following procedure to retract the stripper pins.
For instructions on how to install the Bravo 96AM Head, see the Bravo
Platform Safety and Installation Guide.
CAUTION If the tip-box stripper pins are extended, the pins will crash into the wash
station during syringe washing. To prevent potential equipment damage, ensure that the pins
are always retracted during use.

9
Installing the platform
About installing the AssayMAP head
AssayMAP Bravo Platform Installation Guide
Figure Bravo 96AM Head with tip-box stripper pins retracted (left) and extended (right)
Retracting the tip-box stripper pins
To retract the tip box stripper pins:
1Place the Bravo 96AM Head upside down on a stable surface, so that the
probes are facing up and the front of the 96AM Head (logo side) is facing
you.
If directional arrows appear on the pins, gently rotate the pins so that the
arrows are facing you.
2Insert the end of a 2- mm hex wrench into the head of one of the pins, and
very gently push the pin down into the pipette head until you feel the pin
stop.
Verify that the pin is properly seated. If the pin has an arrow, the square
end of the arrow should disappear or be barely visible.
IMPORTANT If the pin hits a stop before it retracts completely, the pin
locking mechanism is above instead of below the locking dowel. While
removing any downward pressure on the pin head, gently rotate the pin
in quarter- turn increments until you feel the pin drop into the seated
position.
3When the pin is properly seated, rotate it counterclockwise no more than
180°until it locks.
4Repeat this procedure for the other pins.
CAUTION If you apply too much force when pressing down or rotating the pin to retract it,
the locking mechanism at the tip of the pin can bend or break. A damaged pin cannot lock in
the retracted position. Use only gentle pressure to push down and rotate the pin to lock it into
place.

Installing the platform
Installing the 96AM Wash Station and Pump Module
10 AssayMAP Bravo Platform Installation Guide
Installing the 96AM Wash Station and Pump Module
The 96AM Wash Station fits on a platepad with brackets that hold the wash
station securely in place, as the following figure shows.
Figure 96AM Wash Station installed on platepad with brackets
Before you start
Use the following workflow.
Step For this task... See...
1Ensure that the wash station
chimneys are in good
condition.
“Wash station inspection” on
page 11
2Install the bracketed platepad
and the 96AM Wash Station at
deck location 1.
“Installing the 96AM Wash Station
and Pump Module” on page 10
3Set up the tubing
configuration.
“Tubing connections for 96AM
Wash Station” on page 14
4Connect the tubing and the
Pump Module power and
communication cables.
“Routing the tubing” on page 16

11
Installing the platform
Installing the 96AM Wash Station and Pump Module
AssayMAP Bravo Platform Installation Guide
Wash station inspection
Carefully inspect the chimneys in the wash station to ensure that they are:
•In good condition and free of deformities. The chimneys must not be
damaged, such as those shown in the figure (possibly from a head
collision).
•Of equal height. When the chimneys are fully seated in the wash station
their height should be consistent (less than 1 mm difference in height).
If any chimney is taller than the others, use your thumb to press down on
the chimney so that it touches the bottom surface (hard stop) of the wash
station. Use care when seating the chimney to avoid damaging it.
Good condition chimneys and consistent chimney height are critical for proper
wash station function. To replace damaged chimneys, see the AssayMAP 96AM
Wash Station Maintenance Guide.
Figure Close up views of 96AM Wash Station chimneys
Good condition chimneys Damaged chimneys

Installing the platform
Installing the 96AM Wash Station and Pump Module
12 AssayMAP Bravo Platform Installation Guide
Installing the 96AM Wash Station
Before you start
Ensure that you have the following:
•96AM Wash Station and the platepad with brackets
•Pump Module 2.0
•Tubing kit with inline filter
•Hex driver for 2- mm screws
Ensure that you install the 96AM Wash Station at deck location 1.
Figure 96AM Wash Station installed at deck location 1
To install the wash station:
1At deck location 1, remove the standard platepad, if installed, and then
install the bracketed platepad. Use the supplied screw to secure the
platepad to the deck.
Ensure the platepad crosshairs are oriented towards the northwest corner
of the deck, as the following figure shows.
WARNING To prevent potential injury, turn off the Bravo Platform before you install or
remove any accessory.