Agilent Technologies AssayMAP Bravo User manual


AssayMAP
Bravo Platform
Installation Guide
Original Instructions

Notices
© Agilent Technologies, Inc. 2018
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Installation Guide Part Number
G5571-90001
Edition
Revision B, March 2018
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Safety Notices
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hazard. It calls attention to an
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adhered to, could result in personal
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damage to the product or loss of important
data. Do not proceed beyond a CAUTION
notice until the indicated conditions are
fully understood and met.

iiiAssayMAP Bravo Platform Installation Guide
Contents
1. Before you start. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
About this guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Related guides and where to find them. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Safety information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1. Installing the platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Overview of the hardware components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Computer requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Installation workflow for standard configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Installing the Bravo risers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
About installing the AssayMAP head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Installing the 96AM Wash Station and Pump Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Installing the standard platepads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Installing the Peltier Thermal Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Installing and connecting the Orbital Shaking Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
2. Setting up the computer and software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Setting up the computer and installing the software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Turning on the platform and setting up the VWorks software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Verifying the profile settings and configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
About verifying the deck configuration in the profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Verifying the Autofill Station configuration (96AM Wash Station). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Verifying the Peltier Thermal Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Verifying the Orbital Shaking Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Initializing the profile that you are verifying . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Testing the accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
3. Setting the teachpoints . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Teaching part 1: Setting teachpoints in AssayMAP 1 profile. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Teaching part 2: Adjusting teachpoints for accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Teaching part 3: Updating the other profiles based on AssayMAP 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Checking and adjusting the gripper offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
4. Verifying the installation and setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Verification workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
About installing the Bravo pendant, Light Curtain, and shields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Opening the Workbench and accessing the utilities. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Running the Startup utility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Testing the safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Washing the syringes to prepare for first time use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Performing a mock run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Running the Syringe Test utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Running the Shutdown utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

Contents
iv AssayMAP Bravo Platform Installation Guide
Workflow for setting teachpoints in each profile manually. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Adjusting the teachpoint for the Bravo Plate Riser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Adjusting the teachpoint for the red PCR plate insert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

1
AssayMAP Bravo Platform
Installation Guide

1
Before you start
•“About this guide” on page 2
•“Related guides and where to find them” on page 2
•“Service documentation” on page 4
•“Safety information” on page 4

Before you start
About this guide
2AssayMAP Bravo Platform Installation Guide
About this guide
Intent
This installation guide is intended for use by qualified and authorized Agilent
Technologies service personnel who possess an in- depth service background
and have received training in the installation of the AssayMAP Bravo Platform.
What this guide covers
This guide describes the following software versions:
•Protein Sample Prep Workbench v3.0
•VWorks Automation Control v13.1.1
•Bravo Diagnostics v19.1.1
What is new in this revision
and
Related guides and where to find them
Checklists
You can find the following checklists on the Agilent AssayMAP Bravo Platform
product page at https://www.agilent.com/en/products/automation- solutions/
protein- sample- preparation/assaymap- bravo- platform
•Site Preparation Checklist. Specifies the site requirements that must be
met before installing the AssayMAP Bravo Platform.
•Installation Checklist. Lists the tasks to be performed to install the
AssayMAP Bravo Platform.
Feature and description See…
Revised the startup procedure to match
instructions in the Protein Sample Prep
Workbench v3.0.
“Running the Startup utility” on
page 83
Reorganized the workflow for testing the
safety equipment.
“Testing the safety equipment” on
page 84
Revised the syringe wash procedure to
specify 60 wash cycles.
“Washing the syringes to prepare
for first time use” on page 86

3
Before you start
Related guides and where to find them
AssayMAP Bravo Platform Installation Guide
User guides
The following table lists the user guides and where to find them.
To access the VWorks Knowledge Base, do one of the following:
•From within VWorks software, select Help > Knowledge Base or press F1.
•From the Windows desktop, select Start > Agilent Technologies >
VWorks Knowledge Base.
•Go to the online VWorks Knowledge Base at www.agilent.com/chem/askb
and click the link for PDF user guides.
Guide Description Where to find the guide
Bravo Platform Unpacking
Guide
Unpacking instructions for the Bravo
Platform
Attached to shipping
container and in VWorks
Knowledge Base
Bravo Platform Safety and
Installation Guide
Safety guidelines, site specifications,
and installation procedures
Included in the shipping
container and in VWorks
Knowledge Base
Bravo Platform User Guide Setting up, operating, maintaining,
and troubleshooting the Bravo
Platform
VWorks Knowledge Base and
Protein Sample Prep
Workbench
Pump Module User Guide Setting up the Pump Module and the
autofilling reservoirs and wash
stations
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP Bravo Platform
Error Recovery Guide
Error messages, and how to handle
them
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP Bravo Platform
Labware Reference Guide
Labware photo reference and
descriptions
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP Bravo Syringe
Replacement User Guide
Replacing a defective syringe in the
Bravo 96AM Head
VWorks Knowledge Base and
Protein Sample Prep
Workbench
AssayMAP 96AM Wash
Station Maintenance Guide
Replacing chimneys in the 96AM
Wash Station
VWorks Knowledge Base
VWorks Automation Control
Setup Guide
Defining labware and labware classes,
liquid classes, and pipetting
techniques, and how to track and
manage labware in storage
VWorks Knowledge Base
VWorks Automation Control
User Guide
Creating and editing VWorks
protocols and application interfaces
VWorks Knowledge Base
AssayMAP Syringe Test
Utility User Guide
Running a syringe test of the Bravo
96AM Head
Utility instructions in the
Protein Sample Prep
Workbench

Before you start
Safety information
4AssayMAP Bravo Platform Installation Guide
To access the context-sensitive help feature in the VWorks software:
1In the main window of the VWorks software, click the help button .
The pointer changes to . Notice that the different icons or areas are
highlighted as you move the pointer over them.
2Click an icon or area of interest. The relevant topic or document opens.
Service documentation
Agilent field service engineers can find instructions on how to maintain and
repair the Bravo Platform in the following guides:
•Bravo Platform Service Guide
•Pipette Head Service Guide
Changes to existing procedures and additional service procedures are published
as service bulletins and posted in the Support Portal at
http://intranet.chem.agilent.com/sites/partners/support/default.aspx
Safety information
Before installing and using the Bravo Platform, make sure you are aware of the
potential hazards and understand how to avoid being exposed to them. You
must be properly trained in the correct and safe installation and operation of
the device. For details, see the Bravo Platform Safety and Installation Guide.
Do not substitute parts or make any unauthorized modifications to the Bravo
Platform or the supplied safety equipment.
WARNING Changing or modifying the Bravo Platform safety equipment may prevent
the safe operation of the device, invalidate its safety compliance, and lead to personal
injury or property damage. Any customer who does not use the supplied safety equipment
or who modifies the supplied safety equipment assumes full responsibility for providing an
appropriate level of safety for its operators and for providing the applicable safety
compliance marking and documentation.

1
AssayMAP Bravo Platform
Installation Guide

1
Installing the platform
•“Overview of the hardware components” on page 2
•“Computer requirements” on page 3
•“Installation workflow for standard configuration” on page 4
•“Installing the Bravo risers” on page 5
•“Installing the 96AM Wash Station and Pump Module” on
page 10
•“Installing the standard platepads” on page 20
•“Installing the Peltier Thermal Station” on page 21
•“Installing and connecting the Orbital Shaking Station” on
page 26

Installing the platform
Overview of the hardware components
2AssayMAP Bravo Platform Installation Guide
Overview of the hardware components
The following figure and table show the components of the AssayMAP Bravo
Platform, which is required for running the AssayMAP protocols.
Figure AssayMAP Bravo Platform components
Item Required hardware
1Gripper upgrade
2Bravo 96AM Head
396AM Wash Station
4Pump Module 2.0 and two carboys
596AM Cartridge & Tip Seating Station
6Risers, 146 mm
7Peltier Thermal Station with STC controller
8Custom plate nest
9Red PCR Plate Insert
10 Orbital Shaking Station with Control Unit