Agilent Technologies 850-DS User manual

850-DS
Dissolution Sampling Station
Operator’s Manual

Notices
Manual Part Number
70-9074 Rev D
March 2018
Copyright
© Agilent Technologies, Inc. 2018
No part of this manual may be
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(including electronic storage and retrieval
or translation into a foreign language)
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as governed by United States and
international copyright laws.
Agilent Technologies, Inc.
3501 Stevens Creek Blvd.
Santa Clara, CA 95052 USA
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly
performed or adhered to, could
result in damage to the product or
loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated
conditions are fully understood
and met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly
performed or adhered to, could
result in personal injury or death.
Do not proceed beyond a
WARNING notice until the
indicated conditions are fully
understood and met.

850-DS Operator’s Manual 3
Content
1Safety 7
Electrical Hazards 8
Warning 9
Caution 9
Note 9
Information Symbols 10
2 Introduction 11
Serial Number Format 13
3 Setting Up the 850-DS 15
Initial Setup 16
Equipment Ratings / Environmental Requirements for Installation 17
Specification 18
Equipment, Parts, and Accessories As Shipped 19
Parts and Accessories 19
Connections 20
Tubing 21
Power Cord 22
Apparatus Control Cable 22
Synchronized Start 23
Initial Power Up 24
Sample Tray Installation 24
Updating 850-DS Language Screens 25

Content
4850-DSOperator’sManual
Optional Filter Module 27
Changing Trays / Needle Replacement 28
4 Operating the 850-DS 29
Startup Screen 30
Main Screen 32
Menu Screens 36
Methods 36
Method Properties (Apparatus 1/2/5/6) 38
Sample Collection Parameters (Apparatus 1/2/5/6) 40
Method Properties (Apparatus 3/7) 43
Timepoint Number Selection 46
Timepoint Parameter Entry (Apparatus 1/2/5/6) 47
Timepoint Parameter Entry (Apparatus 3/7) 48
Run Method Screen 1 49
Run Method Screen 2 50
Run Method Screen 3 52
Run Method Screen 4 53
Run Method Screen 5 53
850-DS Method Transfer (to another 850-DS) 54
Alarms 56
Calibration 57
Cleaning 61
Diagnostics 64
Diagnostics Screen 2 66
Diagnostics Screen 3 67
Diagnostic Screen 4 (Apparatus 1/2/5/6) 69
Diagnostic Screen 4 (Apparatus 3/7) 71
Instrument Settings 72
Instrument Settings Screen 2 74
Manufacturing Configuration 78
Reports 79
User Access 80

Content
850-DS Operator’s Manual 5
Dialog Screens (Blue) 83
Warning Screens (Yellow) 85
Error Screens (Red) 88
5 Maintenance and Troubleshooting 91
Preventive Maintenance 92
Clean System 92
Periodic Maintenance 92
Printer Maintenance 93
Removing a Thermal Paper Roll 93
Inserting a Thermal Paper Roll 95
Thermal Printer Test 98
Obtaining Warranty and Other Services 99

Content
6850-DSOperator’sManual
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850-DS Operator’s Manual 7
1Safety
Electrical Hazards 8

1Safety
8850-DSOperator’sManual
The Agilent 850-DS Dissolution Sampling Station has been designed and tested
so that when used properly you have an accurate, fast, flexible, and safe
instrument.
If the equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.
Operation of a Agilent 850-DS involves the use of aqueous liquids and various
pharmaceutical dosage forms. Unskilled, improper, or careless use of this
instrument can create shock hazards, fire hazards, or other hazards which can
cause death, serious injury to personnel, or severe damage to equipment and
property.
Information on safety practices is provided with your instrument and operation
manuals. Before using your instrument or accessories, you must thoroughly read
these safety practices.
Observe all relevant safety practices at all times.
Electrical Hazards
The dissolution apparatus contains electrical circuits, devices, and components
operating at dangerous voltages. Contact with these circuits, devices, and
components can cause death, serious injury, or painful electric shock.
Panels or covers that are retained by fasteners which require the use of a tool for
removal may be opened only by Agilent-trained, Agilent-qualified, or
Agilent-authorized service engineers. Consult the manuals or product labels
supplied with the dissolution apparatus to determine which parts are
operator-accessible.
Application of the wrong supply voltage, connection of the instrument to an
incorrectly wired supply outlet, or lack of proper electrical grounding can create a
fire hazard or a potentially serious shock hazard and could seriously damage the
instrument and any attached ancillary equipment.
Always use a three-wire outlet with ground connection which is adequately rated
for the load. The installation must comply with local, state, and federal safety
regulations.
Do not connect the instrument to the main power supply until you have made
sure that the operating voltage is correctly set for the main power supply in the
specific outlet in your laboratory to which the equipment will be connected.

1Safety
850-DS Operator’s Manual 9
Warning
WARNING
A ‘Warning’ message appears in the manual when failure to observe instructions
or precautions could result in death or injury.
Read all warnings and cautions carefully and observe them at all times.
Caution
CAUTION
A ‘Caution’ message appears in the manual when failure to observe instructions
could result in damage to equipment (Agilent supplied and / or other associated
equipment).
Note
NOTE
A ‘Note’ appears in the manual to give advice or information.

1Safety
10 850-DS Operator’s Manual
Information Symbols
A depressed power button in the front of the
unit (with green light) indicates main power
on.
An extended power button in the front of the
unit (with green light off) indicates main
power off.
Indicates single-phase alternating current
Indicates the product complies with the
requirements of one or more European Union
(EU) directives.
Indicates specific equipment meets
standards of safety. These products are safe
for use in the workplace for North America.
Indicates that this product must not be
disposed of as unsorted municipal waste.
All Agilent products that are subject to the
WEEE directive shipped after August 13,
2005 are compliant with the WEEE marking
requirements. Such products are marked
with the “crossed out wheelie bin” WEEE
symbol in accordance with European
Standard EN 50419.
For more information on collection, reuse,
and recycling systems, please contact your
local/regional waste administration, your
local distributor, or Agilent.
Indicates the product complies with
regulatory compliance requirements of New
Zealand and Australia.

850-DS Operator’s Manual 11
2Introduction
Serial Number Format 13

2Introduction
12 850-DS Operator’s Manual
The 850-DS allows you to autonomously collect samples from a dissolution
apparatus. Samples may be collected into various sample holders including 15
mL test tubes, 2 mL vials, and 96-well plates. The vials and well plates may be
conveniently placed on a sample tray that is compatible with Agilent HPLC
autosamplers.The 850-DS provides two basic modes of collecting samples from
a dissolution apparatus: manual control and method control. Manual control
provides full control of the instrument through the Diagnostics screens. You can
independently control all of the features of the instrument.
Method control allows you to enter all the parameters relevant to a sample
collection; prime, sample, and purge volumes, sample collection times, and self
cleaning. If using an Agilent dissolution apparatus, the 850-DS will fully control
the dissolution apparatus through a serial interface.
CAUTION
In manual control as it is possible to pump volumes greater than
those that the sample vessels may hold.
WARNING
The dissolution apparatus contains electrical circuits, devices,
and components operating at dangerous voltages. Contact with
these circuits, devices and components can cause death,
serious injury, or painful electric shock.
CAUTION
Panels or covers that are retained by fasteners which require the
use of a tool for removal may be opened only by Agilent-trained,
Agilent-qualified, or Agilent-authorized service engineers.

2Introduction
850-DS Operator’s Manual 13
Serial Number Format
The serial number contains 10 characters and follows this syntax:
CC1234xxxx
Syntax Code Meaning Description
CC Country of origin 2 alpha characters matching the required trade designation for the
country of origin
12 Year of manufacture ‘16’ for 2016, ‘17’ for 2017, etc.
34 Week of manufacture ‘01’ for week 1 to ‘52’ for week 52

2Introduction
14 850-DS Operator’s Manual
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850-DS Operator’s Manual 15
3 Setting Up the 850-DS
Initial Setup 16
Equipment, Parts, and Accessories As Shipped 19
Connections 20
Optional Filter Module 27
Changing Trays / Needle Replacement 28

3 Setting Up the 850-DS
16 850-DS Operator’s Manual
Initial Setup
Complete the following sections to initially set up the Agilent 850-DS and all other
system components.
1Prepare an area next to a dissolution apparatus where the Agilent 850-DS will
be placed. The preferred location is on the right side of the dissolution
apparatus.
2Remove the Agilent 850-DS and all other system components from the
packing material and place on a bench or table.
3Inspect the equipment and accessories to ensure there has been no damage
during shipment.
4Orient the Agilent 850-DS and all other system components appropriately.
Height 40 cm (60 cm with Filter Module)
Width 39.0 cm (48.0 cm with filter waste bin)
Depth 60.0 cm

3 Setting Up the 850-DS
850-DS Operator’s Manual 17
Equipment Ratings / Environmental Requirements for
Installation
•Electrical Supply: 100-240 V˜ ±10%, 50/60 Hz, max. 2 A
•Temperature: 5 ºC to 40 ºC
•Humidity: max relative humidity 80% for temperatures up to 31 ºC decreasing
linearly to 50% relative humidity at 40ºC
•Altitude: 2000 m
•Pollution Degree: 2
•Installation Category: II
•Indoor use only

3 Setting Up the 850-DS
18 850-DS Operator’s Manual
Specification
* The Sampling Accuracy specification is derived from the following conditions:
10 mL volume, DI H2O, 10 mL/min pump speed and 3-second Aspiration Dwell
Time. This percentage may change based on the sampling parameters or
volume, product formulation and type of dissolution media or when in line filters
are used.
Parameter Specification
Operating Environment 5 to 40 °C
Humidity (non-condensing) Not more than 80% RH
Power requirements Voltage: 90-250 V, 50-60 Hz
Current: 2.5-1.0 A
Dimensions Height: 40.0 cm
Height with filter module: 60 cm
Width: 39.0 cm
Width with filter waste bin: 48.0 cm
Depth: 60.0 cm
Weight 27 kg
Interface 7” color touchscreen (English, Simplified Chinese, Spanish, Japanese, German,
Hungarian, Portuguese, Russian) or PC controlled
Pumping system and speed Rotary piston syringe pump, 8 syringes with adjustable 6-12 mL/min.
Sampling accuracy 2.5% *
Sample volume per vial/tube 0.1-14 mL
(up to 28 mL with dual sample)
Sample frequency Minimum of 2 minutes (under certain conditions)
Number of samples per test Up to 36 (with manual tray exchange after every 12 samples)
Maximum run time 999 hours

3 Setting Up the 850-DS
850-DS Operator’s Manual 19
Equipment, Parts, and Accessories As Shipped
Parts and Accessories
Locate the following items, as applicable, for your system configuration:
Base Unit Components
Part Number Quantity Description
G7930-64000 1 850-DS base unit
K1005-02066 1 Rack for 15mL test tubes
K3090-00155 7 1/4-28 Male to 1/8" barb fitting
3090-0160 8 1/4-28 Male to 1/4-28 Male tubing adapter
33-9019 3 25’ of 1/8" silicone tubing
K1003-00232 1 2.5mm Hex key
K1003-00233 1 3mm Hex key
K1003-00234 1 1/4" slotted screwdriver
K1003-00235 1 #1 Phillips screwdriver
1150-7997 1 Stylus for touchscreen
17-5001 1 Box of 100 15mL test tubes
5075-0042 1 DB25 communications cable
77-0001 1 Safety Manual and Documentation CD
Power cord 1 Country-specific power cord
Optional Accessory Kits
Part Number Quantity Description
K1005-05187 1 850-DS printer kit
G7931A 1 850-DS filtration module
K1001-01202 1 Kit for 2, 54-position LC trays, 2mL vials
K1001-01203 1 Kit for 100-position LC tray, 2mL vials
K1001-01204 1 Kit for well plate LC tray, 2 mL x 96 wells
K1005-05212 1 Sample tray for 2mL vials (8 x 12)

3 Setting Up the 850-DS
20 850-DS Operator’s Manual
Connections
Complete the following sections to connect the necessary tubing and cables for
the Agilent 850-DS.
Figure 1. 850-DS Tubing Connections