Agilent Technologies Cary 8454 User manual


Agilent Cary 8454
UV-Visible Spectroscopy
System
Installation Guide

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
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G1115-90048
Edition
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Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 3
In This Guide…
This handbook describes what you have to do to install your UV-Visible
spectroscopy system. Most of the handbook deals with installing a
complete system, that is, the spectrophotometer, computer and printer.
However, in the second half of this handbook you will find additional
information about installing accessories, accessory boards, GPIB
connections and configuration, and software installation.
1 Fast Installation
Use this chapter as an overview of the different actions you must perform
to install your UV- Visible spectroscopy system. If you are an experienced
user of analytical equipment from Agilent Technologies, this may be all
you need. If you need more information, this chapter guides you to other
relevant areas in this or another handbooks.
2 Installation and Start Up
Use this chapter if you need more detailed information about the
installation process.
3 Good Measurement Practices
Use this chapter if you require information about general operating
conditions to perform accurate and reliable measurements, for example,
handling samples, solvents and cells.
4 Installing Accessories
This chapter describes how to install the simple mechanical and optical
accessories that are available to expand the capabilities of your UV-Visible
spectrophotometer.
5 Installing the UV-Visible Software
This chapter describes what your computer requires, and what you must
do to install the software.

4 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
6 GPIB Communication, Connections and Configuration
This chapter describes how to set the select code, how to install an GPIB
card in your computer and how to change the GPIB address of your
spectrophotometer.
7 LAN Communication, Installation, Connection and Configuration
This chapter describes how to set up LAN communication between your
Agilent ChemStation and your UV-Visible spectrophotometer.
A Safety Information
This chapter contains relevant safety information.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 5
Contents
1 Fast Installation 11
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 12
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer
Systems 13
Installing Software Only 15
2 Installation and Start Up 17
Site Requirements for Your Spectrophotometer 19
Power considerations 19
Power cords 20
Space 20
Environment 21
Unpacking Your Spectrophotometer 22
Installing your UV-Visible Spectrophotometer 23
Instrument overview 23
Setting up the Spectrophotometer 27
Installing Your Computer 29
Setting up the computer 29
Starting the Spectrophotometer 31
Turning On 31
Instrument Warm-up 33
Starting the Agilent ChemStation Software 34
Operational Checkout 34

6 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents
3 Good Measurement Practices 37
General Considerations 38
Spectrophotometer Design 38
Making Measurements 38
Sample Cell or Cuvette Material 39
Optical Specifications of Cells 40
Apertured Cells or Cuvettes 41
Flow Cells 42
Handling and Maintaining Cells or Cuvettes 43
Solvents 45
Sample Preparation 46
Photosensitive Samples 47
Stirring and Temperature Control 47
Checklist for Best Results 48
Inserting a Cell 50
4 Installing Accessories 51
Thermostattable Cell Holder 52
Installing a Thermostattable Cell Holder 53
Cell Stirring Module 54
Installing the Cell Stirring Module 55
Tubing Connections and Operation 56
Long Path Cell Holder 58
Installing the Long Path Cell Holder 59
Installing a Sample Cell 59
Optical Filter Wheel 61
Installing the Filter Wheel Assembly 63
Removing the Filter Wheel Assembly 64
5 Installing the UV-Visible Software 65
Agilent ChemStation Computer Details 66

Contents
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 7
Minimum PC Requirements for Agilent ChemStation 66
Installing the Agilent ChemStation Using the DVD 69
Overview 69
Uninstalling Pre-existing Agilent UV-Visible ChemStation Software 69
Installation Procedure 70
Monitor Configuration 74
Configuring Your System 75
6 GPIB Communication, Connections and Configuration 79
GPIB Communication 80
Operating System-Instrument Compatibility 81
Installing an GPIB Interface 81
GPIB Cabling 82
Installation of the Agilent GPIB Communication Library 84
Installing New IO Libraries 84
Configuring the Agilent 82350 GPIB Interface 88
Configuring the Agilent 82357 USB - GPIB Interface 94
Upgrading Existing IO Libraries 103
Upgrading IO Libraries 103
Working with your Agilent IO Libraries 104
Troubleshooting 105
Missing files during installation 105
Problems with Plug and Play option GPIB cards 107
Changing the GPIB Address of the Spectrophotometer 109
GPIB Address Settings 111
7 LAN Communication, Installation, Connection and Configuration 113
Installing and Configuring TCP/IP on the PC 114
Installation of the TCP/IP protocol 114

8 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents
Installing the LAN Interface in your UV-Visible Spectrophotometer 117
Connecting the Spectrophotometer to the PC 118
Single Instrument Installation 118
Multiple Instrument Installation 118
Setting the IP Address of the Spectrophotometer 119
Using the Agilent BootP Service 120
What is the Agilent BootP Service? 120
Addresses 121
Using the Agilent BootP Service Program 122
Configure the Agilent BootP Service Program 123
Configure the Agilent BootP Service 125
Configuring the G1369C LAN Card Using BootP 127
Using the Agilent ChemStation Configuration Editor to Configure the
Spectrophotometer 129
What is the Agilent ChemStation Configuration Editor? 129
Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer 130
Troubleshooting LAN Communication 133
Power failure reported on the Agilent ChemStation 133
Frequent buffer overrun in Agilent Cary 8454 logbook 134
A Safety Information 135
Safety Information 136
General 136
Operation 136
Safety Symbols 137
Warning and Caution Messages 138
Warning Symbols 138
CE Compliance 140
Electromagnetic Compatibility 141
Solvent Information 143

Contents
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 9
Flow Cells 143
Solvents 143
Lithium Batteries Information 145
Agilent Technologies on the Internet 146
Index 147

10 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents