Agilent Technologies Cary 8454 User manual


Agilent Cary 8454
UV-Visible Spectroscopy
System
Installation Guide

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
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Manual Part Number
G1115-90048
Edition
03/14
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Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 3
In This Guide…
This handbook describes what you have to do to install your UV-Visible
spectroscopy system. Most of the handbook deals with installing a
complete system, that is, the spectrophotometer, computer and printer.
However, in the second half of this handbook you will find additional
information about installing accessories, accessory boards, GPIB
connections and configuration, and software installation.
1 Fast Installation
Use this chapter as an overview of the different actions you must perform
to install your UV- Visible spectroscopy system. If you are an experienced
user of analytical equipment from Agilent Technologies, this may be all
you need. If you need more information, this chapter guides you to other
relevant areas in this or another handbooks.
2 Installation and Start Up
Use this chapter if you need more detailed information about the
installation process.
3 Good Measurement Practices
Use this chapter if you require information about general operating
conditions to perform accurate and reliable measurements, for example,
handling samples, solvents and cells.
4 Installing Accessories
This chapter describes how to install the simple mechanical and optical
accessories that are available to expand the capabilities of your UV-Visible
spectrophotometer.
5 Installing the UV-Visible Software
This chapter describes what your computer requires, and what you must
do to install the software.

4 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
6 GPIB Communication, Connections and Configuration
This chapter describes how to set the select code, how to install an GPIB
card in your computer and how to change the GPIB address of your
spectrophotometer.
7 LAN Communication, Installation, Connection and Configuration
This chapter describes how to set up LAN communication between your
Agilent ChemStation and your UV-Visible spectrophotometer.
A Safety Information
This chapter contains relevant safety information.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 5
Contents
1 Fast Installation 11
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 12
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer
Systems 13
Installing Software Only 15
2 Installation and Start Up 17
Site Requirements for Your Spectrophotometer 19
Power considerations 19
Power cords 20
Space 20
Environment 21
Unpacking Your Spectrophotometer 22
Installing your UV-Visible Spectrophotometer 23
Instrument overview 23
Setting up the Spectrophotometer 27
Installing Your Computer 29
Setting up the computer 29
Starting the Spectrophotometer 31
Turning On 31
Instrument Warm-up 33
Starting the Agilent ChemStation Software 34
Operational Checkout 34

6 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents
3 Good Measurement Practices 37
General Considerations 38
Spectrophotometer Design 38
Making Measurements 38
Sample Cell or Cuvette Material 39
Optical Specifications of Cells 40
Apertured Cells or Cuvettes 41
Flow Cells 42
Handling and Maintaining Cells or Cuvettes 43
Solvents 45
Sample Preparation 46
Photosensitive Samples 47
Stirring and Temperature Control 47
Checklist for Best Results 48
Inserting a Cell 50
4 Installing Accessories 51
Thermostattable Cell Holder 52
Installing a Thermostattable Cell Holder 53
Cell Stirring Module 54
Installing the Cell Stirring Module 55
Tubing Connections and Operation 56
Long Path Cell Holder 58
Installing the Long Path Cell Holder 59
Installing a Sample Cell 59
Optical Filter Wheel 61
Installing the Filter Wheel Assembly 63
Removing the Filter Wheel Assembly 64
5 Installing the UV-Visible Software 65
Agilent ChemStation Computer Details 66

Contents
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 7
Minimum PC Requirements for Agilent ChemStation 66
Installing the Agilent ChemStation Using the DVD 69
Overview 69
Uninstalling Pre-existing Agilent UV-Visible ChemStation Software 69
Installation Procedure 70
Monitor Configuration 74
Configuring Your System 75
6 GPIB Communication, Connections and Configuration 79
GPIB Communication 80
Operating System-Instrument Compatibility 81
Installing an GPIB Interface 81
GPIB Cabling 82
Installation of the Agilent GPIB Communication Library 84
Installing New IO Libraries 84
Configuring the Agilent 82350 GPIB Interface 88
Configuring the Agilent 82357 USB - GPIB Interface 94
Upgrading Existing IO Libraries 103
Upgrading IO Libraries 103
Working with your Agilent IO Libraries 104
Troubleshooting 105
Missing files during installation 105
Problems with Plug and Play option GPIB cards 107
Changing the GPIB Address of the Spectrophotometer 109
GPIB Address Settings 111
7 LAN Communication, Installation, Connection and Configuration 113
Installing and Configuring TCP/IP on the PC 114
Installation of the TCP/IP protocol 114

8 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents
Installing the LAN Interface in your UV-Visible Spectrophotometer 117
Connecting the Spectrophotometer to the PC 118
Single Instrument Installation 118
Multiple Instrument Installation 118
Setting the IP Address of the Spectrophotometer 119
Using the Agilent BootP Service 120
What is the Agilent BootP Service? 120
Addresses 121
Using the Agilent BootP Service Program 122
Configure the Agilent BootP Service Program 123
Configure the Agilent BootP Service 125
Configuring the G1369C LAN Card Using BootP 127
Using the Agilent ChemStation Configuration Editor to Configure the
Spectrophotometer 129
What is the Agilent ChemStation Configuration Editor? 129
Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer 130
Troubleshooting LAN Communication 133
Power failure reported on the Agilent ChemStation 133
Frequent buffer overrun in Agilent Cary 8454 logbook 134
A Safety Information 135
Safety Information 136
General 136
Operation 136
Safety Symbols 137
Warning and Caution Messages 138
Warning Symbols 138
CE Compliance 140
Electromagnetic Compatibility 141
Solvent Information 143

Contents
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 9
Flow Cells 143
Solvents 143
Lithium Batteries Information 145
Agilent Technologies on the Internet 146
Index 147

10 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents

11
Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide
Agilent Technologies
1
Fast Installation
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 12
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible
Spectrophotometer Systems 13
Installing Software Only 15
Installation is straightforward, but if you have difficulties, or need more
information, references to detailed installation information are given.
Use this chapter if you are:
•installing a preconfigured UV-Visible spectroscopy system from Agilent
(including spectrophotometer, computer and software),
•installing a UV-Visible spectroscopy system not purchased as a
preconfigured system, or
•installing software only.

12 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
1 Fast Installation
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems
If you have purchased one of these systems, the software is pre- installed
and preconfigured. You need only install and switch on the hardware.
✔Ensure you have space and an appropriate power supply.
For more information, see “Site Requirements for Your
Spectrophotometer” on page 19.
✔Unpack the system—check for damage and contents against packing list.
For more information, see “Unpacking Your Spectrophotometer” on
page 22.
✔Install your UV-Visible spectrophotometer.
•Connect line power to the rear.
•Connect the waste tubing underneath the sample pan.
For more information, see “Installing your UV-Visible
Spectrophotometer” on page 23.
✔Install your computer and printer.
•Connect the keyboard and mouse.
•Connect the monitor to line power and to the computer.
•Connect the printer to line power and to the computer.
•Connect the computer to the spectrophotometer with the GPIB or
LAN cable.
For more information, see “Installing Your Computer” on page 29 and
Chapter 7, “LAN Communication, Installation, Connection and
Configuration”.
✔Switch on the spectrophotometer (make sure the sample area is clear),
wait 2 minutes for the lamps to ignite. When you are ready to operate
your spectrophotometer, switch on the computer and printer.
For more information, see “Starting the Spectrophotometer” on page 31.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 13
Fast Installation 1
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible
Spectrophotometer Systems
You must install and configure the software. In addition, you may need to
install and configure an instrument communication interface such as the
GPIB PCI interface card.
✔Ensure you have space and an appropriate power supply.
For more information, see “Site Requirements for Your
Spectrophotometer” on page 19.
✔Unpack the system - check for damage and contents against packing
list.
For more information, see “Unpacking Your Spectrophotometer” on
page 22.
✔Install your UV-Visible spectrophotometer.
•Connect the line power to the rear.
•Connect the waste tubing underneath the sample pan.
For more information, see “Installing your UV-Visible
Spectrophotometer” on page 23.
✔If your computer and printer are not supplied by Agilent, ensure that
the requirements for proper operation of the software are met.
For more information, see “Minimum PC Requirements for
Agilent ChemStation” on page 66.
✔Install the GPIB interface board in your computer.
For more information, see “Installing an GPIB Interface” on page 81.
✔Install your computer and printer.
•Connect the keyboard and mouse.
•Connect the monitor to the power supply and to the computer.
•Connect the printer to the power supply and to the computer.
•Connect the computer to the spectrophotometer with the GPIB cable.

14 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
1 Fast Installation
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems
For more information, see “Installing Your Computer” on page 29.
✔Switch on the spectrophotometer (make sure sample area is clear) and
wait two minutes for the lamps to ignite. When you are ready to
operate your spectrophotometer, switch on the computer and printer.
For more information, see “Starting the Spectrophotometer” on page 31.
✔Install the Agilent ChemStation software.
For more information, see “Installing the Agilent ChemStation Using the
DVD” on page 69.
✔From the Windows Start menu, start the Agilent ChemStation software.
For more information, see “Starting the Agilent ChemStation
Software” on page 34.
✔Ensure that your software and spectrophotometer operate properly by
taking a blank measurement or performing the automatic self- test in
the diagnostic section of your software.
For more information, see “Operational Checkout” on page 34.
✔If you need information about solvents, sample preparation, blank
measurements, cuvette handling or how to insert a cuvette, see
Chapter 3, “Good Measurement Practices”.
✔Install any accessories.
For simple accessories see Chapter 4, “Installing Accessories”; for
complex accessories, see the instructions supplied with the accessory or
the Installation and Maintenance section of the Agilent ChemStation
online help.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 15
Fast Installation 1
Installing Software Only
Installing Software Only
✔Unpack the software—check for damage and check the contents against
the packing list.
✔Ensure that your computer and printer meet the requirements for
proper operation of the software.
For more information, see “Minimum PC Requirements for
Agilent ChemStation” on page 66.
✔Switch on the spectrophotometer (make sure the sample area is clear)
and wait two minutes for the lamps to ignite. When you are ready to
operate your spectrophotometer switch on the computer and printer.
✔From the Windows menu install the Agilent ChemStation software.
For more information, see “Installing the Agilent ChemStation Using the
DVD” on page 69.
✔From the Windows Start menu start the Agilent ChemStation software.
For more information, see “Starting the Agilent ChemStation
Software” on page 34.
✔Ensure that your software and spectrophotometer operate properly, by
taking a blank measurement or by performing the automatic self- test in
the diagnostic section of your software.
For more information, see “Operational Checkout” on page 34.

16 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
1 Fast Installation
Installing Software Only

17
Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide
Agilent Technologies
2
Installation and Start Up
Site Requirements for Your Spectrophotometer 19
Unpacking Your Spectrophotometer 22
Installing your UV-Visible Spectrophotometer 23
Installing Your Computer 29
Starting the Spectrophotometer 31
Starting the Agilent ChemStation Software 34
Operational Checkout 34
The following topics are covered in this section:
•“Site Requirements for Your Spectrophotometer” on page 19 contains
information about electrical requirements such as power lines, power
cords, etc. It also gives recommendation for minimum available bench
space and environmental conditions in the laboratory.
•“Unpacking Your Spectrophotometer” on page 22 contains information
about what to do when the packing material is damaged or the
shipment is incomplete. It includes a list of the items which are
supplied with the instrument and computer.
•“Installing your UV-Visible Spectrophotometer” on page 23 gives an
overview of the instrument and explains buttons, indicators and the
line power switch. It explains where and how to connect line power,
communications and waste drain tubing.
•“Installing Your Computer” on page 29 describes how to install the
computer and peripherals, e.g. monitor, mouse, keyboard and printer.
Because the UV-Visible operating software comes already installed when
the system has been ordered as a bundle, this topic is covered in
Chapter 6, “GPIB Communication, Connections and Configuration”.

18 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
2 Installation and Start Up
•“Starting the Spectrophotometer” on page 31 describes the turn- on
procedure for the spectrophotometer and the warm up time required to
fulfill all specifications.
•“Starting the Agilent ChemStation Software” on page 34 describes how
you start Windows from the MS- DOS operating system and how you
start your Agilent ChemStation software.
•“Operational Checkout” on page 34—use this section to ensure that your
spectrophotometer, computer and the UV-Visible operating software is
in proper operating condition. There is an optional automated self- test
which can be used for a quick check of the spectrophotometer. This
self- test can only be executed after the instrument has warmed up for
at least one hour.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 19
Installation and Start Up 2
Site Requirements for Your Spectrophotometer
Site Requirements for Your Spectrophotometer
Power considerations
Your spectrophotometer can operate on a line voltage of 100–240 V AC
with a line frequency of 50 or 60 Hz. Maximum power consumption is
220 VA.
The instrument power supply accepts any line voltage supplied in the
above mentioned range. Consequently there is no voltage selector in the
rear of the spectrophotometer. There are no externally accessible fuses,
because automatic electronic fuses are implemented in the power supply.
The computer and InkJet or LaserJet printers accept a nominal line
voltage of 110 or 220 V AC with a line frequency of 50 or 60 Hz. For
more information, see the documentation that comes with your computer
and/or printer.
WARNING
To disconnect the instrument from line power, disconnect the power cord from the
supply. The power supply still uses some power, even if the power switch on the
front panel is turned off.
WARNING
Connecting your instrument to a line voltage that is higher than specified causes a
shock hazard, and damage may occur to your instrument.

20 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
2 Installation and Start Up
Site Requirements for Your Spectrophotometer
Power cords
Different power cords are offered as options with the spectrophotometer.
The female end of each of the power cords is identical. This connects to
the power- input socket at the rear of the spectrophotometer. The male end
of each of the power cords is different and designed to match the wall
socket of a particular country or region.
Do not position the equipment so that it is difficult to operate the
disconnecting device.
Space
The spectrophotometer is 34.4 cm (13.5 inches) wide, 56.0 cm
(22.0 inches) deep and 18.5 cm (7.3 inches) high (see Figure 1 on
page 24). It weighs 14 kg (30.86 lbs) and will fit on almost any desk or
laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on
either side to allow for the circulation of air for cooling, and
approximately 8 cm (3.1 inches) in the rear for electrical connections.
The computer, including the keyboard, is approximately 50 cm
(19.7 inches) wide and 60 cm (23.6 inches) deep.
Additional space is required for accessories like pumps and autosamplers.
NOTE
Do not replace the mains power cord with one that is inadequately rated. When replacing
the mains power cord, use only a mains power cord with a rating equivalent to the one
supplied with the instrument. See the instrument installation guide for detailed mains
power cord rating information.
WARNING
Always operate your instrument from a power outlet which has a ground
connection. Always use the power cord designed for your region.