Agilent Technologies 1260 Infinity II Quaternary System User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II Quaternary
System
Manual and Quick Guide

1260 Infinity II Quaternary System - Manual and Quick Guide
Notices
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Manual Part Number
G7111-90300
Edition
04/2016
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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1260 Infinity II Quaternary System - Manual and Quick Guide 3
In This Book
In This Book
This book describes the Agilent InfinityLab LC Series 1260 Infinity II
Quaternary System.
1Introduction
This chapter gives an introduction to the Agilent 1260 Infinity II LC, the
underlying concepts and the features of the Agilent 1260 Infinity II LC.
2 Configuration Settings
This chapter describes how to configure the system.
3QuickStartGuide
This chapter provides information on running an Agilent 1260 Infinity II LC
System.
4 Parts and Consumables
This chapter provides information on additional parts and consumables.
5 Appendix
This chapter provides addition information on safety, legal, and web.

4 1260 Infinity II Quaternary System - Manual and Quick Guide
Contents
Contents
1Introduction 5
Product Description 6
Features of the Agilent 1260 Infinity II Quaternary LC 7
System Components 8
Solutions 20
Optimizing the Stack Configuration 24
Leak and Waste Handling 30
2 Configuration Settings 37
General Information on LAN Configuration 38
Instrument Configuration 39
Lab Advisor 41
3QuickStartGuide 47
Best Practices 48
Prepare a Run 49
Check Out the System 56
4 Parts and Consumables 69
Tool Kit 70
A-Line Quick Connect and Quick Turn Fittings 72
5 Appendix 77
Safety 78
Agilent Technologies on Internet 84

5
1260 Infinity II Quaternary System - Manual and Quick Guide
Agilent Technologies
1
Introduction
Product Description 6
Features of the Agilent 1260 Infinity II Quaternary LC 7
System Components 8
Quaternary Pump G7111B 9
Quaternary Pump VL G7111A 10
Isocratic Pump G7110B 11
Multisampler G7167A 12
Vialsampler G7129A 13
Multicolumn Thermostat G7116A 14
Diode Array Detector HS G7117C 15
Diode Array Detector WR G7115A 16
Variable Wavelength Detector G7114A 17
Fluorescence Detector G7121A 18
Refractive Index Detector G7162A 19
Solutions 20
Walk-up 20
Online SPE 20
1260 Infinity II Multimethod Solution 22
Optimizing the Stack Configuration 24
Agilent A-Line LC Flex Bench 24
One Stack Configuration 26
Two Stack Configuration 28
Leak and Waste Handling 30
Waste Guidance 34
Waste Concept 35
This chapter gives an introduction to the Agilent 1260 Infinity II LC, the
underlying concepts and the features of the Agilent 1260 Infinity II LC.

6 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
Product Description
Product Description
The Agilent 1260 Infinity II LC System is the trusted platform, taking you to
the next level of routine analysis, and giving you the instrument choice to
achieve best operational efficiency.
A broad range of reliable instrumentation matches with latest column
technologies guarantees robust separation and detection performance.
Highest instrument utilization and a fast turnaround cycle is achieves through
easy column handling and superior sample logistics from sample submission
to data analysis.
The designed for method transferability and stepwise upgrade capability
enables a risk-free integration in current infrastructure matching your budget.

1260 Infinity II Quaternary System - Manual and Quick Guide 7
Introduction
1
Features of the Agilent 1260 Infinity II Quaternary LC
Features of the Agilent 1260 Infinity II Quaternary LC
With an operating pressure of up to 600 bar the 1260 Infinity II Quaternary
Pump is compatible with HPLC and UHPLC, i.e. handling 2.1, 3, and 4.6 mm ID
columns over the flow rate range (up to 5 mL/min) and semi preparative
analysis due to flow up to 10 mL/min.
Ultralow carryover – The 1260 Infinity II Multisampler is designed for low
carryover, you can take clean to a whole new level with the multi-wash
capability, cleaning all relevant injection parts between runs. This
sophisticated, integrated feature flushes the injection needle outside with
three solvents, and uses seat back flush procedures to reduce carryover to less
than 9 ppm.
Dual-needle injection – By running samples alternately through one or the
other injection path, you can reduce cycle times to mere seconds, virtually
eliminating conventional wait times—whether for large volume loadings or
flushing procedures.
Higher sample capacity per benchspace – Using shallow well-plate drawers,
the 1260 Infinity II Multisampler takes a maximum load of 16 microtiter plates
and up to 6144 samples—the most of any single system.
Advanced column capacity for up to 4 columns in a single 1260 Infinity II
Multicolumn Thermostat delivering best flexibility for column switching.
Fast and easy connections with A-line Quick Connect fittings to save time and
trouble.
1260 Infinity II DAD HS Detector delivers lower detection limit and higher
data quality for more confidence.
Seamless integration in your chromatography data system: Agilent’s
Instrument Control Framework (ICF) enables smooth control of Agilent LC
instrumentation through third-party chromatography data systems.

8 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
System Components
The Agilent 1260 Infinity II LC System consists of the following components:
•Quaternary Pump or Isocratic Pump
•Multisampler or Vialsampler
•Multicolumn Thermostat (MCT)
•Diode Array (DAD), Variable Wavelength (VWD), Refractive Index (RID), or
Fluorescence Detector (FLD)
•Evaporative Light Scattering Detector (ELSD) (optional, not stackable)
•Solvent Cabinet
The Agilent 1260 Infinity II LC is described in more detail in the following
sections. All modules (except the optional ELSD) are stackable, see
“Optimizing the Stack Configuration” on page 24
For specifications, please refer to the individual module user documentation.

1260 Infinity II Quaternary System - Manual and Quick Guide 9
Introduction
1
System Components
Quaternary Pump G7111B
The Agilent 1260 Infinity II Quaternary Pump has an extended power range,
delivering pressures up to 600 bar. At these high pressures you can use
smaller particle size columns and get higher resolution and faster separations.
It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual
floating, precise servo-controlled pistons adjust the stroke volume according
to your chosen flow rate.
Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary
Pump provides the greatest flexibility in automated solvent blending and is
recommended for a wide range of research and routine applications
(especially in the food, environmental and pharmaceutical sectors) as well as
for method development.
Figure 1 Overview of the pump
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10 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
Quaternary Pump VL G7111A
The Agilent 1260 Infinity II Quaternary Pump VL has an extended power
range, delivering pressures up to 400 bar. At these high pressures you can use
smaller particle size columns and get higher resolution and faster separations.
It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual
floating, precise servo-controlled pistons adjust the stroke volume according
to your chosen flow rate.
Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary
Pump VL provides the greatest flexibility in automated solvent blending and is
recommended for a wide range of research and routine applications
(especially in the food, environmental and pharmaceutical sectors) as well as
for method development.
Figure 2 Overview of the pump
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1260 Infinity II Quaternary System - Manual and Quick Guide 11
Introduction
1
System Components
Isocratic Pump G7110B
The Agilent 1260 Infinity II Isocratic Pump is ideal for demanding QA/QC
tasks and routine applications. It maintains virtually pulse-free and stable
solvent flows. The dual floating, precise servo-controlled pistons in the
delivery mechanism adjust the stroke volume according to your chosen flow
rate.
The optional integrated degasser and solvent selection valve offers increased
ease-of-use and method flexibility.
The pump can deliver a broad range of pressures up to a maximum 600 bar,
giving you the flexibility to use small particle size columns, longer columns or
alternative higher viscosity solvents.
The Agilent 1260 Infinity II Isocratic Pump is the ideal pump for GPC/SEC
applications where run-to-run and day-to-day precision in retention times is
crucial.
Figure 3 Overview of the pump
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12 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
Multisampler G7167A
The Agilent 1260 Infinity II Multisampler can handle both vials and microtiter
plates with ease and efficiency up to 600 bar system pressure, optimized on
high flexibility.
This compact module can house up to 6144 samples, all inside the Agilent
stack footprint and the robotics to inject each into the chromatograph in turn.
With the multi-wash capability, you can reduce carryover to less than 9 parts
per million.
Figure 4 Overview of the Multisampler
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1260 Infinity II Quaternary System - Manual and Quick Guide 13
Introduction
1
System Components
Vialsampler G7129A
The Agilent 1260 Infinity II Vialsampler is designed for the reliability, safety,
and ease-of-use needed for routine pharmaceutical tasks and quality control,
as well as for environmental and food analyses. It can house optionally the
integrated column compartment for two LC columns with temperature control
up to 80 °C as well as a sample cooler for stable temperatures down to 4 °C, all
within one module.
Figure 5 Overview of the Vialsampler
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14 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
Multicolumn Thermostat G7116A
The Agilent 1260 Infinity II Multicolumn Thermostat (MCT) allows precise
column thermostatting over a broad temperature range: from cooling down to
10 degrees below ambient temperature up to 85 °C, providing high flexibility
for optimized speed and selectivity of LC separation.
Ultrahigh pressure valves enable a wide range of applications such as column
selection from 4 columns in a single MCT, sample preparation for analyte
enrichment or matrix removal, alternating column regeneration, etc.
For bio-inert applications bio-inert heat exchangers and a selection of
bio-inert valves are offered.
The MCT fits with all 1260 Infinity II modules and can also be combined with
modules of the Agilent 1260 and 1290 Infinity II Series.
Figure 6 Overview of the Multicolumn Thermostat
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1260 Infinity II Quaternary System - Manual and Quick Guide 15
Introduction
1
System Components
Diode Array Detector HS G7117C
The Agilent 1260 Infinity II Diode Array Detector HS is based on the Agilent
Max-Light cartridge cell with optofluidic waveguides that improve light
transmission to near 100 % efficiency without sacrificing resolution caused by
cell dispersions effects.
With typical detector noise levels of < ±0.6 μAU/cm the 60 mm flow cell gives
up to 10times higher sensitivity than detectors with conventional flow cells.
Any compromising refractive index and thermal effects are almost completely
eliminated, resulting in significantly less baseline drift for more reliable and
precise peak integration.
For fast separations, this detector has multiple wavelength and full spectral
detection at sampling rates up to 120 Hz.
Figure 7 Overview of the detector
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16 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
Diode Array Detector WR G7115A
The 1260 Infinity II DAD WR detector is designed for highest optical
performance, GLP compliance, and easy maintenance. With its 120 Hz data
acquisition rate the detector is perfectly suited for fast LC applications. The
long –life deuterium lamps allow highest intensity and lowest detection limits
over a wavelength range of 190 – 950 nm. The use of RFID tags for all flow cells
and UV-lamps provides traceable information about these assemblies.
The built-in holmium oxide filter features the fast wavelength accuracy
verification, while the built-in temperature controls improves the baseline
stability. Additional diagnostic signals for temperature and lamp voltage
monitoring are available.
Figure 8 Overview of the detector
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1260 Infinity II Quaternary System - Manual and Quick Guide 17
Introduction
1
System Components
Variable Wavelength Detector G7114A
The Agilent 1260 Infinity II Variable Wavelength Detector (VWD) is the most
sensitive and fastest detector in its class.
Time-programmable wavelength switching provides sensitivity and selectivity
for your applications.
More sample information can be acquired in the dual wavelength mode.
Low detector noise (<±2.5 μAU) and baseline drift (<1·10
-4
AU/h) facilitates
precise quantification of trace levels components.
High productivity can be achieved with fast analysis at up to 120 Hz data
rates.
Figure 9 Overview of the detector
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18 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
Fluorescence Detector G7121A
The proven optical and electronic design of the Agilent 1260 Infinity II
Fluorescence Detector provides highest sensitivity for the analysis of
trace-level components. Time-programmable excitation and emission
wavelength switching allows you to optimize the detection sensitivity and
selectivity for your specific applications. High-speed detection with up to
74 Hz data rates keeping you pace with the analysis speed of fast LC.
Figure 10 Overview of the detector
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1260 Infinity II Quaternary System - Manual and Quick Guide 19
Introduction
1
System Components
Refractive Index Detector G7162A
The Agilent 1260 Infinity II Refractive Index Detector (RID) is the ideal
detector for fast and reliable LC results when routinely analyzing non-UV
absorbing substances, such as carbohydrates, lipids, and polymers. The 1260
Infinity II RID is also the detector of choice for gel permeation
chromatography (GPC) or size exclusion chromatography (SEC).
Figure 11 Overview of the detector
Status indicator
Interface port area
Service door
Power switch
Leak drain

20 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
Solutions
Solutions
Walk-up
Agilent's Walkup Software enables simplified access to the power and
precision of high quality LC and LC/MS processes for multiple users.
•Users may simply walk up to an LC/MS system, input basic sample
information and choose from a list of analytical methods or purification
schemes available.
•Sample submitters are prompted where to place their samples. They will
receive their results by e-mail when the samples are completed.
•Managers of multiple instrument installations can take advantage of
networking instruments through an OpenLAB Shared Services Server to
allow administration from anywhere in the lab.
Online SPE
Whether you need to enrich your analytes, remove matrix components, or
lower detection limits for e.g. trace-level water analysis, the highly modular
design of the Agilent 1290 Infinty II Series Online SPE Solution provides you
with the flexibility to tailor your system to match virtually any analytical LC
challenge. Agilents Online SPE Solutions are based on the 1290 Infinity
Flexible Cube that houses re-usable SPE cartridges and up to two valves.
Combined with the Agilent 6400 Series Triple Quadrupole mass spectrometers
the 1200 Infinity Series Online SPE Solution allows ultra-low, trace level
detection.
The Online SPE Starter Kit builds the basis for all possible Online-SPE
solutions. In an alternating way you can clean, condition and load your sample
on one of the cartridges while the second cartridge is in the analytical flow
path for analyzing the sample.
In addition to the Online SPE Starter Set the online SPE direct injection kit
allows also to bypass the SPE cartridges and inject directly onto the analytical
column. With this approach you can use your system either for online SPE
analysis or for a direct injection without re-plumbing the system.