Agilent Technologies 1260 Infinity II Quaternary System User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II Quaternary
System
Manual and Quick Guide

1260 Infinity II Quaternary System - Manual and Quick Guide
Notices
© Agilent Technologies, Inc. 2016
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Manual Part Number
G7111-90300
Edition
04/2016
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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1260 Infinity II Quaternary System - Manual and Quick Guide 3
In This Book
In This Book
This book describes the Agilent InfinityLab LC Series 1260 Infinity II
Quaternary System.
1Introduction
This chapter gives an introduction to the Agilent 1260 Infinity II LC, the
underlying concepts and the features of the Agilent 1260 Infinity II LC.
2 Configuration Settings
This chapter describes how to configure the system.
3QuickStartGuide
This chapter provides information on running an Agilent 1260 Infinity II LC
System.
4 Parts and Consumables
This chapter provides information on additional parts and consumables.
5 Appendix
This chapter provides addition information on safety, legal, and web.

4 1260 Infinity II Quaternary System - Manual and Quick Guide
Contents
Contents
1Introduction 5
Product Description 6
Features of the Agilent 1260 Infinity II Quaternary LC 7
System Components 8
Solutions 20
Optimizing the Stack Configuration 24
Leak and Waste Handling 30
2 Configuration Settings 37
General Information on LAN Configuration 38
Instrument Configuration 39
Lab Advisor 41
3QuickStartGuide 47
Best Practices 48
Prepare a Run 49
Check Out the System 56
4 Parts and Consumables 69
Tool Kit 70
A-Line Quick Connect and Quick Turn Fittings 72
5 Appendix 77
Safety 78
Agilent Technologies on Internet 84

5
1260 Infinity II Quaternary System - Manual and Quick Guide
Agilent Technologies
1
Introduction
Product Description 6
Features of the Agilent 1260 Infinity II Quaternary LC 7
System Components 8
Quaternary Pump G7111B 9
Quaternary Pump VL G7111A 10
Isocratic Pump G7110B 11
Multisampler G7167A 12
Vialsampler G7129A 13
Multicolumn Thermostat G7116A 14
Diode Array Detector HS G7117C 15
Diode Array Detector WR G7115A 16
Variable Wavelength Detector G7114A 17
Fluorescence Detector G7121A 18
Refractive Index Detector G7162A 19
Solutions 20
Walk-up 20
Online SPE 20
1260 Infinity II Multimethod Solution 22
Optimizing the Stack Configuration 24
Agilent A-Line LC Flex Bench 24
One Stack Configuration 26
Two Stack Configuration 28
Leak and Waste Handling 30
Waste Guidance 34
Waste Concept 35
This chapter gives an introduction to the Agilent 1260 Infinity II LC, the
underlying concepts and the features of the Agilent 1260 Infinity II LC.

6 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
Product Description
Product Description
The Agilent 1260 Infinity II LC System is the trusted platform, taking you to
the next level of routine analysis, and giving you the instrument choice to
achieve best operational efficiency.
A broad range of reliable instrumentation matches with latest column
technologies guarantees robust separation and detection performance.
Highest instrument utilization and a fast turnaround cycle is achieves through
easy column handling and superior sample logistics from sample submission
to data analysis.
The designed for method transferability and stepwise upgrade capability
enables a risk-free integration in current infrastructure matching your budget.

1260 Infinity II Quaternary System - Manual and Quick Guide 7
Introduction
1
Features of the Agilent 1260 Infinity II Quaternary LC
Features of the Agilent 1260 Infinity II Quaternary LC
With an operating pressure of up to 600 bar the 1260 Infinity II Quaternary
Pump is compatible with HPLC and UHPLC, i.e. handling 2.1, 3, and 4.6 mm ID
columns over the flow rate range (up to 5 mL/min) and semi preparative
analysis due to flow up to 10 mL/min.
Ultralow carryover – The 1260 Infinity II Multisampler is designed for low
carryover, you can take clean to a whole new level with the multi-wash
capability, cleaning all relevant injection parts between runs. This
sophisticated, integrated feature flushes the injection needle outside with
three solvents, and uses seat back flush procedures to reduce carryover to less
than 9 ppm.
Dual-needle injection – By running samples alternately through one or the
other injection path, you can reduce cycle times to mere seconds, virtually
eliminating conventional wait times—whether for large volume loadings or
flushing procedures.
Higher sample capacity per benchspace – Using shallow well-plate drawers,
the 1260 Infinity II Multisampler takes a maximum load of 16 microtiter plates
and up to 6144 samples—the most of any single system.
Advanced column capacity for up to 4 columns in a single 1260 Infinity II
Multicolumn Thermostat delivering best flexibility for column switching.
Fast and easy connections with A-line Quick Connect fittings to save time and
trouble.
1260 Infinity II DAD HS Detector delivers lower detection limit and higher
data quality for more confidence.
Seamless integration in your chromatography data system: Agilent’s
Instrument Control Framework (ICF) enables smooth control of Agilent LC
instrumentation through third-party chromatography data systems.

8 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
System Components
The Agilent 1260 Infinity II LC System consists of the following components:
•Quaternary Pump or Isocratic Pump
•Multisampler or Vialsampler
•Multicolumn Thermostat (MCT)
•Diode Array (DAD), Variable Wavelength (VWD), Refractive Index (RID), or
Fluorescence Detector (FLD)
•Evaporative Light Scattering Detector (ELSD) (optional, not stackable)
•Solvent Cabinet
The Agilent 1260 Infinity II LC is described in more detail in the following
sections. All modules (except the optional ELSD) are stackable, see
“Optimizing the Stack Configuration” on page 24
For specifications, please refer to the individual module user documentation.

1260 Infinity II Quaternary System - Manual and Quick Guide 9
Introduction
1
System Components
Quaternary Pump G7111B
The Agilent 1260 Infinity II Quaternary Pump has an extended power range,
delivering pressures up to 600 bar. At these high pressures you can use
smaller particle size columns and get higher resolution and faster separations.
It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual
floating, precise servo-controlled pistons adjust the stroke volume according
to your chosen flow rate.
Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary
Pump provides the greatest flexibility in automated solvent blending and is
recommended for a wide range of research and routine applications
(especially in the food, environmental and pharmaceutical sectors) as well as
for method development.
Figure 1 Overview of the pump
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10 1260 Infinity II Quaternary System - Manual and Quick Guide
1 Introduction
System Components
Quaternary Pump VL G7111A
The Agilent 1260 Infinity II Quaternary Pump VL has an extended power
range, delivering pressures up to 400 bar. At these high pressures you can use
smaller particle size columns and get higher resolution and faster separations.
It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual
floating, precise servo-controlled pistons adjust the stroke volume according
to your chosen flow rate.
Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary
Pump VL provides the greatest flexibility in automated solvent blending and is
recommended for a wide range of research and routine applications
(especially in the food, environmental and pharmaceutical sectors) as well as
for method development.
Figure 2 Overview of the pump
6WDWXVLQGLFDWRU
3XUJHYDOYH
3RZHUVZLWFK
/HDNGUDLQ
'HJDVVHU
0&*9