Amos AST 560 User manual

AST 560
Semi-automatic Cryostat Microtome
Operation Manual
www.amos-scientific.com
AMOS SCIENTIFIC PTY.LTD. ABN37 159 778 140

Presented in this Operation manual are the structure, functions
and using notice. Reading carefully prior to operating the
instrument.

Foreword
AST560 Cryostat Microtome is a
Semi- automatic microtome whose design
concept comes from customers all over the
world; it can meet different requirements
for tissue section.
To ensure the instrument works
properly, safely and perform durably, it is
necessary to read the operation manual
carefully.
Our companies are not only
responsible for the repair, but also train
distributors' ability of repairing. To
repair the product smoothly in time,
please contact your local distributor.
Notice: With the development of
technology and constantly updating of
products, this manual will make the
corresponding changes. Please forgive us
not to notify the improvement of technical
data and structure.

CONTENTS
1、SAFETY NOTES......................................................................................................................................1
1.1OVERVIEW .............................................................................................................................................................1
1.2 SAFETY WARNING ................................................................................................................................................1
1.3 SAFETY DEVICE ....................................................................................................................................................2
2、PERFORMANCE & PARAMETERS....................................................................................................3
2.1 PRODUCT DESCRIPTION &INTENDED USE.........................................................................................................3
2.2 OVERVIEW-INSTRUMENT COMPONENTS ...........................................................................................................3
2.3 PERFORMANCE INDEX .........................................................................................................................................4
2.4 TECHNICAL PARAMETER .....................................................................................................................................4
3、PREPARATION BEFORE OPERATING.............................................................................................5
3.1 INSTALLATION SITE REQUIREMENT ....................................................................................................................5
3.2 STANDARD DELIVERY...........................................................................................................................................5
3.3 INSTALLATION.......................................................................................................................................................6
3.4 ELECTRICAL CONNECTION ..................................................................................................................................7
4、OPERATION ...........................................................................................................................................8
4.1 OPERATION PANEL FUNCTION &CONTROL ......................................................................................................8
4.3 AUXILIARY FUNCTION....................................................................................................................................... 10
4.4 CRYO CHAMBER TEMPERATURE SETTING........................................................................................................ 11
4.5 SPECIMEN CLAMP TEMPERATURE SETTING .................................................................................................... 12
4.6 SECTION,TRIMMING AND RETRACTION SETTING.......................................................................................... 13
4.7 LOCK SCREEN KEY &AUXILIARY FUNCTION DISPLAY .................................................................................. 14
4.8 BLADE HOLDER INSTALLATION ....................................................................................................................... 14
4.9 CLAMPING THE SPECIMEN AND INSERTING THE BLADE................................................................................. 17
4.10 SECTION REFERENCE ..................................................................................................................................... 18
5、CLEANING & MAINTENANCE......................................................................................................... 18
5.1 CLEANING........................................................................................................................................................... 18
5.2 CLEANING THE WASTE TISSUE.......................................................................................................................... 19
5.3 MAINTENANCE .................................................................................................................................................. 21
6、TROUBLE SHOOTING....................................................................................................................... 21

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1、Safety Notes
1.1Overview
The Operator Manual contains important safety instructions and information. In order to
operate the instrument safely, the operator must read carefully prior to starting up the instrument.
It is very important to ensure safety and avoid instrument damage. Please keep the manual close
to the instrument to read easily at any time.
▲Note: The safety marks and protective device on both instrument and accessories may
neither be removed nor modified , so as not to injure human body or instrument .
1.2 Safety Warning
Every principle person should read carefully and carry out strictly according to the following
safety notes relating to transport, installation, calibration, operation, maintenance, cleaning and
all aspects of the instrument.
1.2.1 Warning during transport and installation
●The instrument may only be transported or moved in an upright position and the tilt
angle should not exceed 45 °.
●Prior to removing the microtome , remove the blade holder .
●The input voltage has been preset at the factory , please check this setting complies with
your local power requirement before connecting to the main power
●Please use the power cord provided . If need to replace it, please use the power cord
with earth wire.
●Do not operate this instrument in the room with the danger of explosion .
●Don't remove or modify the protective device on both instrument and accessories , in
order to avoid injury to instrument or human body !
1.2.2 Warning of Operation
●Take care when handling microtome knives and disposable blades . The cutting edge is
extremely sharp and might cause serious injury.
●Remove the knife/blade before detaching the blade holder from the instrument , and put
the blade back into the blade box when not in use
●Never place the blade with the cutting edge facing upwards and never take out the blade
with your hands.
●Please clamp the specimen block before clamping the blade .
●Prior to replacing the specimen and blade , please lock the handwheel and cover the
blade guard on the cutting edge .
●Rotate the handwheel clockwise, otherwise it would affect section .
●Don't rotate the handwheel clockwise and counterclockwise frequently at top and
bottom , it might lead to deviation of section thickness .
●Ensure no liquid enters into interior of instrument during working .

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1.2.3 Warning during cleaning and Maintenance
●Only authorized person may do the service and repair .
●Before cleaning and maintenance of each part, please cut off the power , unplug the
power plug , remove the blade holder and blade .
●Lock the handwheel before cleaning.
●Don't clean the instrument with corrosive liquid .
●Ensure no liquid enters into interior of instrument during clearing .
●After cleaning , don't turn on the instrument before it is completely dry .
●Turn the instrument off and unplug the power cord before changing the fuse . Please use
the fuse according to the requirement and specification in operation manual.
1.3 Safety Device
Handwheel locking mechanism
As showed in Fig1, it is the lock lever. Clockwise push the lever (1) until it locks the
handwheel at the upright position. To unlock the handwheel, rotate counterclockwise the lever
(1).
As showed in Fig1, the handwheel is locked. The "Lock" indicator light will be lit on display
panel as fig 2..
●Don't lock the handwheel when the handwheel is rotating , otherwise it will damage the
instrument
▲Attention: Before removing the instrument , changing specimen/blade or cleaning the
instrument ,the handwheel must be locked .
Fig 1 Fig 2
1
The lock indicator is highlighted,
indicating the handwheel is locked.

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2、Performance & Parameters
2.1 Product description & intended use
Cryostat Microtome are usually composed of control systems , mechanical systems , drive
systems , blade holder , blade , housing etc , which is for the pathological analysis of tissue
samples .
2.2 Overview-Instrument Components
The main structure of cryostat microtome is shown in Figure 3 as below:
1
Touch Panel
7
Heating glass
2
Left control panel
8
Specimen Clamp
3
Freeze Shelf
9
Blade holder
4
Stationary heat extractor
10
Tool Shelf
5
Peltier on freeze shelf freezing area
11
Lock lever
6
Waste container
12
Handwheel
1
12
2
3
4
5
6
8
9
10
11
7
Fig 3

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2.3 Performance Index
AST 560 is a Semi-automatic cryostat microtome whose specimen forward and backward
movement is controlled by motor. So it can get high quality section and easily operated. The
following is its performance:
⊙The whole instrument follows human engineering theory as well as artistic appearance,
made by numerical control machine.
⊙Artificial intelligence interface touch panel is easy to learn and operate.
⊙The retraction function can prevent the specimen from touching the blade sharp during lift.
⊙It equips counter which is able to show total quantity of slice.
⊙Adopt UV and O3disinfection to sterilize for 30 minutes every time.
⊙The specimen clamp semiconductor refrigerating function is enabled or disabled
⊙Defrosting at fixed time or manually
⊙Sterilization at fixed time or manually
⊙Sleep at fixed time or manually
⊙Large freezing shelf could load 17 samples simultaneously.
⊙Quickly feeding function.
⊙Waste section suction function.
⊙Alarm information query.
2.4 Technical Parameter
⊙Environment requirement：Temperature Range：+15℃～+30℃，
Air Humidity：≤60%
Working pressure：（86～106）kPa；
⊙Nominal Voltage：220～240V AC/100～120V AC
⊙Normal Frequency：50/60 Hz
⊙Power：≤1000VA
⊙Fuse：8A/20A
⊙Safe Classify：Classify I - Type B
⊙Lowest freeze chamber Temperature ：-35±2℃
⊙Lowest freeze shelf Temperature：-45±5℃
⊙Lowest temperature of peltier unit on freeze shelf：-55±5℃
⊙Lowest temperature of peltier unit on specimen clamp：-50±5℃(Working time of
peltier :15 minutes)
⊙Electric coarse feed：Slow 300µm/s±30µm/s
Rapid 900µm/s±30µm/s
⊙Section thickness range：0 to 100µm
0 to 3µm，in 0.5µm increments
3 to 10µm, in 1µm increments
10 to 20µm, in 2µm increments
20 to 100µm，in 5µm increments

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⊙Trimming thickness range: 0 to 600µm
0 to 50µm，in 5µm increments
50 to 100µm, in 10µm increments
100 to 600µm, in 50µm increments
⊙Retraction ：0~80µm, in 5µm increments
⊙Specimen feed：24mm ±0.2mm
⊙Vertical stroke：54mm±1mm
⊙Specimen Max：35×35mm
⊙Repositioning of blade holder base(left-right) ：50±1mm
⊙Motorize cutting speed : 75～230mm/s ±10% .
⊙Dimension: Width：715mm ,
Length：765mm ,
Height：1230mm ,
⊙Weight: About 130kgs
3、Preparation before operating
3.1 Installation site requirement
⊙The floor stand instrument fixed 4 wheels and 2 fixed support feet . The front only be
used during movement. Remove the package and move the instrument to the installation
site, then adjust the two fixed support feet and make the front wheels disabled.
⊙Start the instrument after let it rest for at least 2 hours .
⊙Ensure no other goods around the instrument . At least 300mm distance must be kept for
ventilation and heat dissipation.
⊙Ensure the working temperature and humidity must be in accord with the specification in
manual .
⊙Ensure leaving enough running space for rotating the handwheel .
▲Notice: Do not operate the instrument in the room with explosion hazard .
3.2 Standard Delivery
AST560 Cryostat Microtome
1set
7mm Wrench
1pc
Blade holder
1set
Disposable Blade
1box
Handwheel
1pc
Power Cord
1pc
Specimen clamp
18pcs
Fuse
2pcs
M5 Allen Key
1pc
Brush
1pc
M3Allen Key
1pc
Operation Manual
1pc
●Please check the supply carefully with the packing list after opening the carton . If you
have any doubt, contact the seller immediately .If you have any special requirement, please
illuminate it before the order.