BANDELIN SONOCOOL 255.2 User manual

Operating Instructions
51806-001 en/2021-12
C
High-power ultrasonic bath
SONOCOOL 255.2

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Copyright & limitation of liability
This document may not be reproduced, whether in full or in part, without the prior approval of BANDELIN electronic GmbH & Co. KG,
hereinafter "BANDELIN".
The German language original is the binding version of this document. Any differences in the translation are not binding and have
no legal effect. In case of any discrepancies between the translation and the original of this document, the original version will take
precedence.
BANDELIN accepts no responsibility or liability for damage caused by improper handling or usage contrary to the intended purpose.
The documentation has been prepared with great care. Liability for indirect or direct damages arising because of incomplete
or erroneous information in this documentation, as well as in its delivery and usage, is excluded.
All illustrations are provided as examples and are not true to size. Decorative elements are not included in the scope of delivery.
Subject to technical alterations. Measurements are subject to manufacturing tolerances.
© 2021
1 2 GmbH & Co. KG, Heinrichstraße 3 – 4, Germany, 12207 Berlin,
Tel.: [phone] - 0, Fax: [phone], [email protected]

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General
The device, the accessories and the agents are to be used in accordance with
the operating instructions and/or the product information.
The instructions are part of the scope of delivery and are to be stored in the vicinity
of the device for later reference. This also applies if possession of the device is transferred
elsewhere.
Before the device is put into operation, these instructions are to be read carefully and
completely in order for the user to become familiarised with all functions.
The warnings and safety precautions (Section 1.5) are always to be followed during use.
The manufacturer will not assume any responsibility for the device's safety or functional
ability in the event of improper handling or usage contrary to the intended purpose.
In the event of unauthorised alterations/modifications, both the warranty claim and
the CE conformity will become void.
If service is required, please contact the authorised dealer or the manufacturer.
Symbols used:
Symbol Meaning Explanation
Danger Denotes information that, if not observed, could pose a risk
to life and limb, especially from electric shock.
Caution
Identifies information that must be observed and complied with
in order to prevent damage to the device or injury to the user.
When device parts are labelled with this symbol,
the documentation must be referred to.
Warning Warning of hot surface.
Warning
This symbol indicates hazards caused by flammable
materials. Failing to follow these materials poses a danger
to the life and health of persons.
Important Identifies information that is important for execution.
Note Identifies explanatory information.
Do not grab inside For health reasons, touching the oscillating liquid is prohibited.
Wear hearing
protection
For health reasons, spending long periods of time in
the vicinity of the device without hearing protection is prohibited.
Instructions Identifies instructions that must be carried out in
the described sequence.

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Contents
1 Product description ......................................................................................................6
1.1 Intended use ................................................................................................................8
1.2 Mode of operation ........................................................................................................8
1.3 CE conformity ...............................................................................................................8
1.4 Technical data ..............................................................................................................9
1.5 Warnings and safety instructions ...............................................................................10
2 Preparation .................................................................................................................12
2.1 Scope of delivery ........................................................................................................12
2.2 Setup / Assembly .......................................................................................................13
2.3 Commissioning ...........................................................................................................13
3 Operation ...................................................................................................................14
3.1 Operating elements ....................................................................................................14
3.1.1 Turning the ultrasonic bath on/off...............................................................................14
3.1.2 Explanation of the display fields .................................................................................15
3.2 Signals on the ultrasonic bath ....................................................................................15
3.3 Operating modes ........................................................................................................16
3.4 Setting the operating parameters ...............................................................................17
3.4.1 Temperature setting ...................................................................................................17
3.4.2 Time setting ................................................................................................................18
3.4.3 Power setting .............................................................................................................18
3.5 Activating / deactivating the ultrasound ......................................................................19
4 Use ..............................................................................................................................20
4.1 Fill the oscillating tank and de-air it ...........................................................................20
4.2 Degassing the liquid ..................................................................................................21
4.3 Ultrasonic operation ...................................................................................................22
4.4 Refresh / replace the contact liquid ............................................................................23
4.5 Further information .....................................................................................................24
4.5.1 Contact liquid .............................................................................................................24
4.5.2 Degassing ..................................................................................................................25
4.5.3 Disposal of sonication liquids .....................................................................................25

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5 Cleaning and maintenance of the ultrasonic bath ......................................................26
5.1 Cleaning and care ......................................................................................................26
5.2 Disinfection .................................................................................................................27
5.3 Warehousing / Storage ...............................................................................................27
6 Service and repair ......................................................................................................28
6.1 User maintenance ......................................................................................................28
6.1.1 Check LCD display .....................................................................................................28
6.1.2 Cleaning the cooling system ........................................................................................28
6.1.3 Monitoring the ultrasound.............................................................................................29
6.1.4 Check the cooling.........................................................................................................30
6.1.5 Checking the ultrasound effect.....................................................................................30
6.2 Maintenance by manufacturer or qualified personnel ..................................................32
6.2.1 Visual inspection ..........................................................................................................33
6.2.2 Functional check ..........................................................................................................34
6.2.3 Pre-cleaning .................................................................................................................34
6.2.4 Replace components ...................................................................................................34
6.3 Malfunction/error analysis ............................................................................................36
6.4 Repair...........................................................................................................................38
6.4.1 Replacing fuses............................................................................................................38
6.4.2 Determining information on software version and report data .....................................38
6.5 Service .........................................................................................................................39
7 Accessories ................................................................................................................40
7.1 Required accessories .................................................................................................40
7.2 Optional accessories ..................................................................................................41
7.3 Chemical agents ........................................................................................................42
8 Consumable materials - not applicable - .....................................................................42
9 Taking the unit out of service ......................................................................................42
10 Keyword index - not applicable - .................................................................................43

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1 Product description
Ultrasonic bath with SONOCOOL type cooling.
The exact type specification and serial number are found on the type plate.
Product features:
• Stainless steel oscillating tank (1) with oscillating systems, ultrasonic frequency 35 kHz
• Filling level mark for safe filling (2), electronic fill level sensor (2a)
• Operating and display panel (3) with LCD display (4)
• Lid (5), recess/support for glass lid (5a)
• Tank inlet (6) and outlet (7)
• Shut-offvalve for emptying (8), protective cap (8a) in front of emptying nozzle
• Mains switch (9), mains connector (10) and
• Exhaust air opening (11)
5a
4
3
5
8a
8

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11
9
10
2
2a
7
6
1

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1.1 Intended use
The SONOCOOL ultrasonic bath is intended for use in laboratories and facilities for
industrial and scientific research as well as material testing institutes. With the bath
it is possible to use the catalytic effect of ultrasound for processes during which
simultaneous cooling is required. Heat-sensitive samples are protected by the cooling
function and process sequences can be designed to be faster and more effective than
customary procedures.
The device is intended for commercial use.
The target group for SONOCOOL ultrasonic baths is trained specialists in laboratories
or research facilities.
1.2 Mode of operation
With the bath it is possible to use the catalytic effect of ultrasound for processes during
which simultaneous cooling is required. Heat-sensitive samples are protected by
the cooling function and process sequences can be designed to be faster and more
effective than customary procedures.
In addition to the temperature, the runtime can be set for up to 99:59:59 hours.
Four different levels of ultrasonic power can also be selected. The ultrasonic
bath is table-top operated using the keys on the front of the device, and is designed
for unmonitored continuous operation.
1.3 CE conformity
The device satisfies the CE-marking criteria of the European Union:
- Low-Voltage Directive
- Electromagnetic Compatibility Directive
- RoHS Directive
in their currently valid versions.
The declaration of conformity can be requested from the manufacturer.

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1.4 Technical data
SONOCOOL ultrasonic baths are CE certified.
Safety: EN 61010-1,
EMC: EN 61326-1
Oscillating tank, interior: 280 × 150 × 150 mm (L × W × H)
External dimensions: 360 × 605 × 385 mm (L × W × H)
Housing: Coated aluminium
Oscillating tank: Stainless steel (1.4404), 2 mm, welded
Working fill capacity: 5 litres (contact liquid
~4 litres in the tank +
1 litre in the cooling unit)
Adjustable bath temperature: 4–40 °C (in 20 °C environment)
Refrigerant R290
Refrigerant quantity: 0.09 kg
Cooling capacity: 200 W
Ultrasound output: 180 W, adjustable to 4 levels
Power setting 25 %, 50 %, 75 %, 100 %
Ultrasonic frequency: 35 kHz
Countdown operation: up to 100 h
Emptying: front left, concealed
Current consumption: 1.9 A
Rated voltage: 230 V~ (± 10 %), 50 Hz
Cable length: 2 m
Protection class: Class I
Weight (net): 29 kg
Protection class: IP 2X pursuant to EN 60529

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Ambient conditions
Overvoltage category: II
Degree of contamination: 2
Permissible ambient temperature: 5 to 35 °C
Permissible relative humidity: 80 %
Altitude: up to 2000 m above sea level
No dewing.
Only for indoor operation.
Electromagnetic compatibility (EMC)
The device has been checked for electromagnetic compatibility (EMC) in accordance
with DIN EN 61326-1 and meets the requirements for Class B devices in accordance
with EN 55011.
Suitable for use in institutions and other areas that are directly connected to a public
low-voltage power supply, e.g., laboratory facilities.
1.5 Warnings and safety instructions
General
• Keep the ultrasonic bath out of the reach of children and of persons who have not been
instructed in its operation by reference to these instructions.
• We will not offer any guarantee for damages to the ultrasonic bath or oscillating tank,
or to the objects to be treated, as a result of use of inadequate disinfection agents
or detergents.
• Do not expose the ultrasonic bath directly to sunlight and do not position it in the vicinity
of heat sources.
• Keep the surface of the ultrasonic bath and operating elements clean and dry.
• Do not expose the ultrasonic bath to corroding influences.
• Move the ultrasonic bath only when it is empty.
• Empty the ultrasonic bath only while turned off.
• Ultrasonic baths adhere to prescribed EMC limit values, such that it can be assumed
that the electromagnetic radiation emanating from the devices is harmless to humans.
A binding statement for wearers of implants can only be made at the place of work
and together with the implant manufacturer. In case of doubt, information regarding
the allowable electromagnetic exposure level should be obtained from the implant
manufacturer.

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Operation
• Observe ambient and set-up conditions, see Section 1.4.
• Only plug in the ultrasonic bath to an outlet with a grounded socket.
• Do not operate the ultrasonic bath without liquids.
• Do not fill the oscillating tank with hot water (> 30 °C). The filling temperature should
be between 15 and 25 °C.
• Do not stand or lay any objects directly on the tank bottom. Use accessories,
see chapter 7.
• Do not immerse any parts of the body (e.g. hands, feet) or living organisms (animals
or plants) in the tank; in particular, do not make contact with the bath liquid during
ultrasound operation. Danger: Ultrasound has a cell-destroying effect.
• In the event of continuous activity within a 2 m radius, adequate hearing protection must
be used. Danger: Hearing disturbances during operation are possible if not wearing
hearing protection – the typical ultrasound cavitation noise can be very uncomfortable.
• Fully replace used liquids, do not refresh them by adding more liquids. Follow
the manufacturer's dosage instructions.
Cooling
• Information on the cooling unit in the SONOCOOL ultrasonic bath:
- Refrigerant: R290; quantity: 90 g, extremely flammable
• The cooling circuit must not be damaged!
The cooling unit contains flammable coolant!
If the cooling circuit is damaged, immediately disconnect the power plug and ventilate
the installation room well. Send the ultrasonic bath for repair to the manufacturer.
• Never cover or close the incoming or outgoing air openings on the ultrasonic bath.
• In order to protect the refrigeration unit, the compressor will only start after a resting
period of two minutes.
Damage
• If damage to the ultrasonic bath is detected, do not connect the ultrasonic bath
to the mains.
• In the event of defects, disconnect the power plug immediately.
• Repairs must only be conducted by authorised skilled personnel or by the manufacturer.
• Defective parts may only be replaced with original parts.
Service
• Maintenance may only be carried out by qualified personnel.
• Before opening, disconnect the device from the mains.
• While maintenance work is being carried out on the pump,
there is a risk of crushing fingers due to high magnetic forces.

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2 Preparation
Carefully unpack the ultrasonic bath and accessories and inspect them for completeness or
possible transportation damages. Any identified damage or defects must be communicated
immediately and in writing to the transportation company and to the supplier.
Before operation, the ultrasonic bath should be allowed to stand for 2 hours at its operating
location so that it can adjust to the ambient conditions.
2.1 Scope of delivery
Set for pathology (order no.: 3500031), comprising:
1 × ultrasonic bath with integrated cooling unit (order No.: 350003) including:
1 × sample holder PH 255-11
1 × lid D 255 G
20 × inset beakers SD 01.2
1 × drain hose ¼"
1 × mains cable
1 × TICKOPUR TR 3 250 ml
1 Operating instructions
Additional accessories according to order – see delivery note
Set for laboratory (order no.: 3500032), comprising:
1 × ultrasonic bath with integrated cooling unit (order No.: 350003) including:
1 × insert basket K 5 C
1 × drain hose ¼"
1 × mains cable
1 × TICKOPUR TR 3 250 ml
1 Operating instructions
Additional accessories according to order – see delivery note

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2.2 Setup / Assembly
• Place the ultrasonic bath on a firm, level, dry surface, on a location away from direct
sunlight if possible. In the process
- Observe the maximum weight of the ultrasonic bath, including liquid.
For net weight, see technical data in Section 1.4.
- Observe the minimum distances for the incoming and outgoing air.
At least 5 cm on the side and at least 10 cm on the rear.
- Guard against moisture and wetness – risk of electric shock.
• The ultrasonic bath must be positioned in such a way that it is easy to disconnect
the power supply when required.
2.3 Commissioning
Thoroughly rinse the ultrasonic bath oscillating tank with water before its first use.
Note:
All outer surfaces (and also the inner walls of the oscillating tank) are covered with
an oily preservative in order to protect the surface during transport and storage.
This should be removed with a suitable cleanser before first use, see Section 5.
Connect the ultrasonic bath to the power supply (grounded socket).
To conduct a function test, fill and ventilate the oscillating tank in accordance with
chapter 4.1. Briefly activate the ultrasound on the switched-on ultrasonic bath.
A hissing noise should be heard.
It is recommended that a foil test be conducted as part of quality assurance prior
to the first use, see chapter 6.1.5.
Next, empty out and switch offthe ultrasonic bath in accordance with chapter 4.4.
Hang the sample holder in the oscillating tank and place the lid on top.
5 cm
5 cm
10 cm

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3 Operation
3.1 Operating elements
The unit is operated from the front:
12 345
1 LCD display
2 Arrow keys [], [], [], []
3 Key [SET]
4 Key [START/STOP]
5 Status LED
3.1.1 Turning the ultrasonic bath on/off
The ultrasonic bath is turned on/offusing the mains switch on the rear side. After turning
on, the LCD display must light up. Initialisation occurs automatically.
=====================
BANDELIN electronic
* S O N O C O O L *
=====================
- initialising -
Next, the ultrasonic bath will be on stand-by mode and will be ready for operation.
The status LED (5) will be lit up, the most recently-set values for runtime, temperature
and ultrasonic power will be displayed.
(Values when activated for the first time): target temperature 17 °C, target runtime
00:10:00 hh:mm:ss, target power 100 %).
The last-selected parameters (runtime, target temperature, target power) will be saved
and accessed the next time the device is turned on.

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3.1.2 Explanation of the display fields
All relevant operating parameters are shown on the LCD display (e.g.: Stand-by mode).
12:00:00 17.0°C 50%
12:00:00
17.2°C
12:00:00
Runtime
Current (remaining) runtime
for the ultrasound operation.
If [START/STOP] has not yet been pressed,
the time will correspond to the target runtime.
Actual temperature
Actual temperature in the oscillating tank
Power specification
Specified value for the ultrasonic power
during sonication (in %)
Target temperature
Specified value for
the temperature in
the oscillating tank
(in °C)
Target runtime
Specified value for the duration
of the ultrasound operation
(in hh:mm:ss format)
Note:
As soon as the ultrasonic bath is turned on, the cooling will run automatically according
to the set target temperature. If cooling is not desired, it can be temporarily deactivated
by adjusting the target temperature, e.g. by setting the temperature to 30 °C.
The ultrasound is switched on separately (see chapter 3.5).
3.2 Signals on the ultrasonic bath
Meaning of the colours of the status LED
LED Explanation
green Ultrasonic bath is operating faultlessly.
yellow Warning. Ultrasonic bath may still be operated, however.
red Error (see error number). The ultrasonic bath or ultrasound cannot be started.
Newly-occurring errors are notified using a short audible signal and sometimes
with a blinking warning sign .

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3.3 Operating modes
The operation of the SONOCOOL ultrasonic bath is conducted using four different
operating modes.
Name and sample display Brief description / display To get there from…
Stand-by mode Basic state of the ultrasonic bath.
The runtime (shown inverted)
and the current temperature are
displayed in the upper part of
the display. The target values for
the time, temperature and power
are displayed in the bottom part.
The cooling is activated, the
ultrasound is turned off.
- After turning on
+ initialisation
- When the runtime
has elapsed
- When interrupting
a process from
the Pause mode
+ pressing and
holding the [SET]
key for a long time
Editing mode Menu to change the operating
parameters (see chapter 3.4).
The selected parameter and
the current value are shown on
the top portion of the display.
All valid target values are shown
on the bottom rows.
- Stand-by mode
+ [SET] key
- Active mode
+ [SET] key
- Pause mode
+ [SET] key
Active mode Ultrasound is activated.
The residual runtime (remaining
runtime) is shown in non-inverted
format and runs as a second-by-
second countdown. Below it, the
actual temperature is displayed.
All valid target values are shown on
the bottom rows.
- Stand-by mode
+ [START/STOP] key
- Editing mode (from
Stand-by and Pause)
+ [START/STOP] key
- Pause mode
+ [START/STOP] key
Pause mode Interruption of the active mode
The remaining runtime is shown in
inverted form (dark text on a light
background). The process can be
continued thereafter.
The display is similar to the one
for Stand-by mode; however
the message "p-a-u-s-e" appears
on the next to last row.
- Active mode
+ [START/STOP] key
12:00:00 17.0°C 50%
Temperature:
17.2°C17
12:00:00 17.0°C 50%
12:00:00
17.2°C
12:00:00
12:00:00 17.0°C 50%
11:59:57
17.2°C
12:00:00 17.0°C 50%
===== p-a-u-s-e =====
12:00:00
17.2°C
09:15:21

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3.4 Setting the operating parameters
By pressing the [SET] key, the editing mode is activated.
Depending on the operating mode used to call up the editing mode, it will be possible
to change different operating parameters:
Call-up from Editable parameters
Stand-by mode Target runtime, target temperature and target power
Active mode Target temperature and target power
Pause mode Remaining runtime (without changing the target runtime!),
target temperature and target power
It is possible to switch between the individual parameters by pressing the [] key or [] key.
The settings can be changed by pressing the [] and [] keys. Each of the editable
parameter positions is displayed inversely. Changes made become immediately active.
3.4.1 Temperature setting
The temperature can be changed at any time in editing mode.
Setting limits: 4-40 °C
Example: taken from stand-by mode Editing mode (temperature)
12:00:00 17.0°C 50%
Temperature:
17.2°C17
12:00:00 17.0°C 50%
12:00:00
17.2°C
12:00:00
SET
Notes:
• If the actual temperature exceeds the value of the target temperature by more than
+ 1.0 °C, the ultrasonic bath will shut offthe ultrasound for safety reasons (ultrasound
energy heats up the liquid). If the limit value is exceeded before the start, it will not be
possible to activate the ultrasound. The status LED will blink red.
• The ultrasonic bath will independently activate the ultrasound if the actual temperature
is once again within the stated tolerance range.
• The set time will only be counted during active ultrasound emission.
• If the target value for the temperature is higher than the actual temperature,
only a warning will be issued and the ultrasound may be started. The status LED
will blink yellow.
• With a target temperature below 15 °C, the ultrasound may be occasionally switched
offunder normal ambient conditions (approx. 20 °C).
This can be prevented by reducing the ultrasound power setting.

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3.4.2 Time setting
The target runtime can only be edited from stand-by mode. It cannot be edited while
under active operation (active mode) since the ultrasound continues to be active
while in editing mode.
From the pause mode, only the remaining runtime can be edited.
This setting, however, will not change the runtime specification
(saved value in stand-by mode).
Setting limits: 00:00:10 – 99:59:59 (hh:mm:ss)
Example: taken from stand-by mode Editing mode (temperature)
Editing mode (temperature)
12:00:00 17.0°C 50%
Temperature:
17.2°C17
12:00:00 17.0°C 50%
Temperature:
17.2°C17
12:00:00 17.0°C 50%
12:00:00
17.2°C
12:00:00
SET
Editing mode (time)
12:00:00 17.0°C 50%
Time:
12:00:0012:00:00

3.4.3 Power setting
The power can be changed at any time in editing mode.
Setting values: 25 %, 50 %, 75 % and 100 %.
Example: taken from stand-by mode Editing mode (temperature)
Editing mode (temperature)
12:00:00 17.0°C 50%
Temperature:
17.2°C17
12:00:00 17.0°C 50%
Temperature:
17.2°C17
12:00:00 17.0°C 50%
12:00:00
17.2°C
12:00:00
SET
Editing mode (power)
2
12:00:00 17.0°C 50%
Power:
50%50 %


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3.5 Activating / deactivating the ultrasound
• By pressing the "START/STOP" key, the ultrasound is turned on. This will be visually
shown on the display through a countdown of the remaining runtime.
• Pressing the [START/STOP] key once again interrupts the ultrasound emission
(pause mode).
• The ultrasound emission can be resumed (remaining runtime) by pressing
the [START/STOP] key once again in pause mode.
By pressing the [SET] key for 2 sec, the procedure can be interrupted from
pause mode and the display values can be reset (return to stand-by mode).
• When the set time has elapsed, the ultrasonic bath will stop. The time display
will show "00:00:00" and a short audible signal will be emitted. The ultrasonic
bath will be in stand-by mode once again.
Ex.: Stand-by mode Active mode
12:00:00 17.0°C 50%
11:59:57
17.2°C
12:00:00 17.0°C 50%
12:00:00
17.2°C
12:00:00
START
STOP
Active mode Pause mode
12:00:00 17.0°C 50%
09:15:21
17.2°C
12:00:00 17.0°C 50%
===== p-a-u-s-e =====
09:15:21
17.2°C
09:15:21
START
STOP

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4 Use
Depending on the application, the samples are sonicated indirectly in inlet beakers or other
sample vessels, which are placed on special holders and inserted into the contact liquid in
the oscillating tank.
Direct sonication of objects in the bath liquid is not permitted
with the SONOCOOL.
The intended process sequence is described step by step
in the following chapters.
4.1 Fill the oscillating tank and de-air it
In order to transmit the ultrasound between the oscillating tank and the laboratory flasks,
the tank must be filled with a contact liquid composed of normal municipal or deionised
water together with a surfactant, e.g. TICKOPUR TR 3. For additional information, see
chapter 4.5.
Notes - Filling
• Do not fill the oscillating tank with hot water (> 30 °C). The filling temperature should
be between 15 and 25 °C.
• Water of at least drinking quality must be used to fill the oscillating tank.
• Only use distilled or deionised water without additives in inset beakers or insert tubs.
• Do not use any combustible liquids (e.g. benzine, solvents) or chemicals that contain
chloride ions or that separate (some disinfectants, household cleaners, and dish
detergents) for sonication in the stainless steel tank.
• When working with aggressive preparations in inset beakers or insert tubs: Prevent
the contact liquid or stainless steel surfaces from being sprayed. If needed,
replace the contact liquid immediately, clean the surfaces and wipe dry.
• When using preparations, the safety instructions included in the product leaflets
are to be fundamentally adhered to.
Inset beakers in the sample holder
Contact
liquid
Sonication
liquid