Beckman Coulter AU680 Installation guide

AU680 In-Lab Training Manual Page 1 of 152
Version 2.0 (September 2016) Beckman Coulter Professional Development
AU680 Chemistry Analyzer
In-Lab Training Manual
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Version 2.0 (September 2016) Beckman Coulter Professional Development

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Version 2.0 (September 2016) Beckman Coulter Professional Development
AU680 Chemistry Analyzer
WARNINGS AND PRECAUTIONS
Read all product manuals and consult with Beckman Coulter-trained personnel before
attempting to operate the instrument.
Beckman Coulter, Inc. urges its customers and employees to comply with all national
health and safety standards such as the use of barrier protection. This may include,
but is not limited to, protective eyewear, gloves, suitable laboratory attire when
operating or maintaining this or any other automated laboratory equipment.
INTENTION FOR USE
This document is not intended to replace the information in your Instructions for
Use or Reference Manual. Information in the Instructions for Use and Reference
Manual supersedes information in any other manual.
REVISION STATUS
Version 2.0 (September 2016)
Based on:
AU680 Chemistry Analyzer Software version 4.0
AU680 Chemistry Analyzer Instructions for Use B04779AB
AU680 Chemistry Analyzer Reference Manual B63158AA
TRADEMARKS
The following Beckman Coulter trademarks are registered in the USPTO and may be
used in this manual:
Beckman Coulter and the Beckman Coulter logo
AU
All other trademarks are property of their respective owners.

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AU680 Chemistry Analyzer
In-Lab Training Manual
Table of Contents
Chapter 1 Analyzer Overview.......................................................................................7
AU680 Hardware .........................................................................................................8
Sample Processing Overview....................................................................................10
ISE Testing Sequence ...............................................................................................12
Chapter 2 Software Overview.....................................................................................13
AU680 Home Menu ...................................................................................................14
Main Button Area.......................................................................................................15
Home Menu Area.......................................................................................................16
Alarm Area.................................................................................................................17
Menu List ...................................................................................................................18
Software Menu Path ..................................................................................................19
Online Help................................................................................................................20
Chapter 3 Daily Startup...............................................................................................21
Set the Start Conditions.............................................................................................22
Daily Analyzer Maintenance.......................................................................................24
Check the Analyzer Status.........................................................................................28
Check and Replenish Reagents.................................................................................30
ISE Startup – Check the ISE Reagents .....................................................................32
ISE Startup – ISE Daily Maintenance ........................................................................34
Analyzer Calibration...................................................................................................38
Process Quality Control (QC).....................................................................................40
Chapter 4 Sample Processing and Programming....................................................43
Sample Racks............................................................................................................44
Sample Data Prefix....................................................................................................46
AU680 Validated Sample Containers.........................................................................47
Sample Processing....................................................................................................50
Manual Programming.................................................................................................54
Additional Sample Programming Features.................................................................58

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Chapter 5 Monitor and Review Results.....................................................................63
Print RB/CAL/QC Results ..........................................................................................64
Calibration Monitor.....................................................................................................66
QC Monitor and QC Data Review..............................................................................68
Review Patient Results..............................................................................................72
Chapter 6 Maintenance...............................................................................................75
Maintenance Resources ............................................................................................76
Weekly and Every Other Week Maintenance.............................................................77
Monthly Maintenance.................................................................................................85
Chapter 7 As-Needed Tasks.......................................................................................93
Fix (Assign) a Reagent Position.................................................................................94
Set Calibrator Lot Concentrations..............................................................................96
Configure New QC Lot...............................................................................................98
Define a User Menu.................................................................................................100
Save or Load System Parameters...........................................................................102
Save Data to External Media ...................................................................................104
Perform an End Process (System Shutdown)..........................................................106
Perform and Recover from an Emergency Stop ......................................................110
Disable a Test..........................................................................................................112
Transfer to Host.......................................................................................................114
Chapter 8 Troubleshooting ......................................................................................117
Alarms......................................................................................................................118
Flags........................................................................................................................120
Troubleshooting Reagent Blank, Calibration, and QC Failures................................121
Troubleshooting Tips ...............................................................................................125
Chapter 9 Resources ................................................................................................131
Beckman Coulter Support Personnel.......................................................................131
References...............................................................................................................132
Chapter 10 In-Lab Training Competency Exercise.................................................133
In-Lab Training Competency Exercise Reference Key ............................................145

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Chapter 1
Analyzer Overview
Introduction
The AU680 is an automated chemistry analyzer that measures analytes in samples, in
combination with appropriate reagents, calibrators, quality control (QC) material, and
other accessories. The system is for in vitro diagnostic use only. The AU680 performs
automated analysis of serum, urine, other fluids, and whole blood.
This chapter will cover the following:
Analyzer and ISE hardware components
Photometric sample processing overview
ISE sample processing overview
Understanding the function of hardware components in the processing of samples on
the analyzer can assist in efficiently operating the system, as well as with performing
maintenance and troubleshooting.
Note: The ISE module is an optional module of the analyzer. You can skip any ISE-
related information and procedures in the In-Lab Training Manual if your lab does not
have the ISE module.
Practice
Use the information presented in the following pages to identify the hardware
components on the analyzer
Review the sample processing overviews for tests processed in cuvettes
(photometric) and the ISE module
Animations for the reaction process can be found on the Beckman Coulter website.
Use the following pathway to access the e-learning modules:
www.beckmancoulter.com > SUPPORT > Training & Education >
Diagnostics/Chemistry > AU680 > select On Demand tab:
AU680 Overview E-learning: Provides a hardware and software overview of
the AU680 with reaction process animation
AU680 ISE Overview E-learning: Provides a hardware overview of the ISE
module with reaction process animation

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AU680 Hardware
Component Function
1 Rack Feeder
Module Holds racks waiting to process and feeds the racks through the
analyzer for sampling
2 Sample Probe Aspirates sample (in conjunction with the sample syringe) from a
sample container and dispenses into a cuvette or the ISE sample pot
3 Cuvette Wheel Houses 165 glass cuvettes where reactions occur and absorbance
readings are taken
4 R1/R2 Reagent
Refrigerators Two refrigerated compartments that house R1 (60 positions) and R2
(48 positions) reagents
5 R1 Reagent Probe Aspirates and dispenses R1 reagent (in conjunction with the R1
reagent syringe) into glass cuvettes located in the cuvette wheel
6 R2 Reagent Probe Aspirates and dispenses R2 reagent (in conjunction with the R2
reagent syringe) into glass cuvettes located in the cuvette wheel
7 R1/S Mix Bar
Component Spiral-shaped mix bars on the component mix the contents of the
cuvette after R1 reagent and sample are dispensed
8 R2 Mix Bar
Component L-shaped mix bars on the component mix the contents of the cuvette
after R2 reagent is dispensed
9 Photometer Lamp Component of the photometry system that is used to measure
reactions
10 Wash Nozzle
Component Cleans, rinses, and dries cuvettes before and after analysis
11 STAT Table Use to process STAT samples and for ISE calibration and ISE
maintenance
12 ISE Module Measures sodium (NA), potassium (K), and chloride (CL) ions by the
indirect (diluted) ion-selective electrode (ISE) method
13 Rack Collection
Area Collects racks the system no longer needs for operator removal
R1
R1
R2
R2
Used if Auto
Repeat enabled

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AU680 Hardware (Continued)
Component Function
1 Tank Storage Area Houses the DI water tank, wash solution tank, and diluted
wash solution tank; used to clean cuvettes and mix bars
and rinse components
2 R2 Syringe Aspirates and dispenses specified volume of R2 reagent
3 R1 Syringe Aspirates and dispenses specified volume of R1 reagent
4 ISE Buffer Syringe Aspirates and dispenses ISE Buffer reagent
5 ISE Reagents Bulk reagents required for electrolyte analysis
(ISE Reference, ISE MID Standard, ISE Buffer)
6 Sample Syringe Aspirates and dispenses the specified sample volume
7 Wash Syringe Aspirates and dispenses DI water for sample probe
cleaning
8 Operation Buttons
ON (green): Turns on the analyzer and computer
EM STOP (orange): Emergency stop turns off all power
to the analyzer immediately. Ctrl+Alt+Del is required to
turn off the computer
RESET (white): Use following an EM STOP or power
failure before pressing the ON button

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Sample Processing Overview
Step Action
1 A sample rack is placed on the rack feeder module by the operator
2 The operator presses Start
3 The rack is moved to the bar code reader where sample programming is
identified
4 The rack is moved to the sample aspiration position
5
The reagent probe, working with the
reagent syringe, delivers R1 reagent
(concentrated) and diluent into a
cuvette
6 An R1 mix bar mixes the diluted
reagent in the cuvette
7 The photometer starts taking readings
8
The sample probe, working with the
sample syringe, aspirates and
dispenses sample into the cuvette in
the cuvette wheel

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Step Action
9 A sample mix bar mixes the sample and the reagent. The photometer
continues to take readings
10
If required, the reagent probe, working
with the reagent syringe, delivers the
R2 reagent (concentrated) and diluent
into the cuvette
11 An R2 mix bar mixes the reaction
mixture
12 The photometer continues to take
reaction readings
13
The cuvette is washed, rinsed and
dried by the wash nozzle probes using
diluted wash solution and DI water
Note: Each wash nozzle is a 3-way
nozzle used to clean the cuvettes:
Longest aspirates liquid
Middle dispenses liquid
Shortest aspirates overflow liquid
14 When the sample is no longer needed the rack is moved to the rack
collection area
Note: A priority sample may be processed using the STAT table at any time during
routine sample processing. This allows the priority sample to be processed before
samples loaded through the rack feeder module.

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ISE Testing Sequence
Step Action
1
The mixture aspiration roller pump pulls ISE Reference Solution from the bottle
and past the REF electrode where measurements are taken and the solution is
sent to waste.
2
ISE Buffer Solution is delivered to the sample pot by the ISE Buffer syringe.
3
Sample is aspirated by the sample probe and dispensed into the sample pot
where the sample and ISE Buffer Solution are mixed by the mix bar.
4
The mixture aspiration roller pump draws the diluted sample through the flowcell
where measurements are taken. Excess diluted sample in the sample pot is
pulled through the bypass tubing by the mixture aspiration roller pump and sent
to waste.
5
After a urine sample is processed, ISE Buffer Solution is delivered to the sample
pot by the ISE Buffer syringe and pulled through the flowcell by the mixture
aspiration roller pump to rinse the flowcell.
6
After each sample is processed, the MID Standard roller pump pulls ISE MID
Standard Solution from the bottle and delivers it to the sample pot. The mixture
aspiration roller pump draws the ISE MID Standard Solution through the flowcell
to condition the electrodes and take measurements and the solution is sent to
waste.

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Chapter 2
Software Overview
Introduction
The software is used by the operator to interact with the analyzer and perform system
operations.
This chapter will cover the following:
Common software screen areas
Home menu of the AU680
Software screen structure
Accessing the Help menu
Being familiar with the software can assist in understanding and efficiently operating the
system. Three software navigation methods are available for interacting with the
software:
Touch screen
Mouse
Keyboard
Practice
Use the information on the following pages to identify the common software areas
and the Home menu
Access the Help button to identify the information available on the system. Help is
not accessible when the analyzer is in Measure mode
The Main Button area buttons are not labeled on the software. Place the pointer of
the mouse over a button to identify the name of the button

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AU680 Home Menu
Main Button Area
Home Menu
Buttons
Message
Area
Shortcut
Buttons
Alarm
Message
Area
Alarm
Clear Alarm
List

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Main Button Area
The Main Button Area is viewable from all software screens.
Button Description
Home Displays the Home screen
Menu
List Displays the Menu List screen
User
Menu Displays the User Menu screen (operator-defined shortcut
buttons)
Mode
Display
area
Displays the current mode. Displays the time remaining for
certain maintenance operations
Start Starts analysis
Pause Pauses analysis. The system pauses at the first test for which
no reagent was dispensed
Feeder
Stop Stops the rack supply component. The analysis of samples in
racks that are loaded continues
Stop /
Standby Stops analysis. All data in process will be lost. In Stop mode,
select this button to return the system to Standby mode
Help The system displays a menu for accessing the operator
documentation and maintenance video directory. This button
is not available when the system is in Measure mode
Logout Logs out an operator
End Shuts down the system (End Process). Shutting down the
system turns off the auxiliary power supply, including the lamp
and computer
Time
Display
area Displays the current date and time
.

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Home Menu Area
Home
Menu Button Description
Sample Status: Displays the sample status under analysis,
estimated time of completion, and results
Analyzer Status: Displays the analyzer status and temperatures
Simple STAT Mode: Processes STAT samples one at a time with
minimal operator actions required. Samples cannot be processed in
the normal analyzer operation modes when using Simple STAT Mode
Message Area Description
Displays messages regarding system conditions that
can affect analysis results
Colors indicate the severity level of the message:
Red
You cannot start analysis until you address
red messages
Orange
You can start analysis. Review the message
carefully and take corrective actions
Yellow
You can start analysis. Review the message
carefully and take corrective actions. Yellow
messages will shift to orange (more severe)
if they are not addressed
Green
A notification of system status. The system
has no operational problems
A symbol displays in the upper left of the screen
according to the most severe message color
When a message is selected from the message area:
A system displays a dialog with information and
corrective actions for the message
Highlights the affected area on the analyzer diagram

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Home Screen Shortcut Buttons
Shortcut buttons provide direct menu access to the most frequently used menus to
simplify software navigation.
Button Description
Set a new data index, the group of tests in use, the operator
name, and start sample numbers
Displays the reagent status and cleaning solution status
Displays the analyzer and ISE maintenance schedules. Use to
start some maintenance procedures
Displays sample information and manually order (requisition)
tests for patient samples, calibration, and QC
Start and monitor priority STAT samples for analysis from the
STAT table; manually order (requisition) STAT samples
View and print reagent blank, calibration, QC, and patient data,
and batch transfer data to the laboratory information system (LIS)
Alarm Area
Displays alarm messages generated during system operation.
Button Description
Alarm Clear Select to stop the audible alarm. Select a second time to
clear the alarm message from the screen
Alarm List Select to display the Alarm List. The system can store
and display a maximum of 999 alarms

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Menu List
This column displays the software menus.
The selected menu is highlighted in blue. This column displays the submenus for the selected menu.
Menu Description
Routine Perform various basic operational procedures: set start conditions,
manage reagents, order samples/calibrations/QC, view/print results,
review reaction data
Calibration View history of calibration information and perform calibration
verification
QC View and manage QC data and charts
Parameters Program information for all tests, including calibration and QC
parameters
Maintenance Monitor and perform maintenance, review a detailed alarm log, and
perform diagnostic functions
System Program online conditions, list formats, comments, bar code options,
system settings, and data management

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Software Menu Path
1 Menu List
2 Menus
3 Submenus
4 Screens
5 Tabs
6 Buttons for corresponding function keys (F1 – F8 on the keyboard)

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Online Help
Select the Help button from the Main Button area to access the AU680 Chemistry
Analyzer Online Help Main Menu
Online Help includes:
PDF versions of Instructions for Use and Reference Manual
Video Directory with videos of select maintenance procedures
Tips for using Online Help:
Accessible only when system is not in Measure mode
PDF documents can be printed – be sure to enter a page range before printing if
only printing a specific section or procedure
Blue text within the PDF document can be selected as a link to other procedures
Exiting and returning to the Online Help window takes you back to the Main Menu
– it does not recall the last page you were on