Beckman Coulter LH 780 User manual

COULTER®LH 780 System
Special Procedures
and Troubleshooting
PN 773023AC (December 2010)
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
LH 780
POWERON
POWEROFF
STARTUP
SHUT DOWN
ALARM
RESET
PRIME
APERT
DRAIN RINSE CLEAR
APERT
STOP PREMIX START
CONT
E
N
T
E
R
123
6
45
9
8
7
.
CE
0
FID
VII
VI
V
IV
III
II
I
VIII
NEC
MultiSync LCD 1700m+

WARNINGS AND PRECAUTIONS
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING
TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL
INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER’S RECOMMENDATIONS. IF IN DOUBT AS
TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY
STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,
PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR
ANY OTHER AUTOMATED LABORATORY ANALYZER.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Can cause injury.
CAUTION - Can cause damage to the instrument.
IMPORTANT - Can cause misleading results.
WARNING Risk of operator injury if:
r All doors, covers and panels are not closed and secured in place prior to and during instrument operation.
r The integrity of safety interlocks and sensors is compromised.
r Instrument alarms and error messages are not acknowledged and acted upon.
r You contact moving parts.
r You mishandle broken parts.
r Doors, covers and panels are not opened, closed, removed and/or replaced with care.
r Improper tools are used for troubleshooting.
To avoid injury:
r Keep doors, covers and panels closed and secured in place while the instrument is in use.
r Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors.
r Acknowledge and act upon instrument alarms and error messages.
r Keep away from moving parts.
r Report any broken parts to your Beckman Coulter Representative.
r Open/remove and close/replace doors, covers and panels with care.
r Use the proper tools when troubleshooting.
CAUTION System integrity might be compromised and operational failures might occur if:
r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals.
r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s
computer with software authorized by Beckman Coulter.
r You install software that is not an original copyrighted version. Only use software that is an original copyrighted
version to prevent virus contamination.
IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter
distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot
guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most
current information bulletins concerning the product. If you purchased this product from a third party and would like
further information concerning this topic, call your Beckman Coulter Representative.

iii
PN 773023AC
REVISION STATUS
Issue A, 12/06
Software Version 1A. Converted from Help Version 1A.063001 (CE)
Issue AA, 08/09
Software Version 1B1. Converted from Help Version 1B1.091732.
Issue AB, 10/10
Software Version 1B.1.
Updates were made to the company corporate address.
Issue AC, 12/10
Software Version 1B.1.
Changes were made to:
r FLUSHING LOW VACUUM LINES
r FLUSHING THE BACKWASH TANK VACUUM SUPPLY LINE
r USING HANDHELD SCANNER
r Added troubleshooting step to Backwash Tank Not Full.
Note: Changes that are part of the most recent revision are indicated in text by a bar in the
margin of the amended page.
This document applies to the latest software listed and higher versions. When a subsequent software version
changes the information in this document, a new issue will be released to the Beckman Coulter website. For
labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for
your instrument.

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REVISION STATUS

PN 773023AC v
CONTENTS
WARNINGS AND PRECAUTIONS
REVISION STATUS, iii
INTRODUCTION,xv
HOW TO USE YOUR COULTER®LH 780 SYSTEM HARD-COPY MANUALS,xv
ABOUT THIS MANUAL,xv
ONLINE HELP SYSTEM,xvi
CONVENTIONS,xvi
1 CALIBRATION,1-1
1.1 OVERVIEW,1-1
When to Calibrate,1-1
1.2 PERFORMING REPRODUCIBILITY CHECK,1-1
Sample Requirements,1-1
1.3 REVIEWING REPRODUCIBILITY RESULTS,1-2
Reproducibility Limits for CBC,1-3
1.4 PERFORMING CARRYOVER CHECK,1-3
Carryover Formula,1-4
1.5 REVIEWING CARRYOVER RESULTS,1-4
1.6 CALIBRATING CBC PARAMETERS WITH S-CAL®CALIBRATOR,1-4
Summary,1-4
Procedure,1-5
A Ensure Apertures are Clean,1-5
B Ensure the Instrument is Functioning Properly,1-5
C Prepare the Instrument for Calibration,1-5
D Set Up the CBC Calibration Information at the Workstation,1-6
E Run S-CAL Calibrator,1-6
F Review Results on the Calibration Window,1-6
G Adjust Parameters As Needed,1-8
H On the Command Center, Select AUTO ANALYSIS as the Process Type,1-8
I Verify Calibration by Cycling Each Level of Coulter 5C®Cell Control in Automatic
Aspiration Mode,1-8
1.7 CALIBRATING CBC PARAMETERS WITH WHOLE BLOOD,1-8
Sample Requirements,1-8
1.8 CHANGING CALIBRATION FACTORS FOR AUTOMATIC ASPIRATION MODE,1-9
1.9 CHANGING CALIBRATION FACTORS FOR MANUAL ASPIRATION MODE,1-10
1.10 RECEIVING AVERAGE CALIBRATION FACTORS FROM WORKSTATION,1-11
1.11 SETTING UP NEW CALIBRATION INFORMATION,1-11

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CONTENTS
1.12 REVIEWING ANALYZER'S CURRENT CALIBRATION FACTORS FOR AUTOMATIC
ASPIRATION MODE,1-11
1.13 REVIEWING ANALYZER'S CURRENT CALIBRATION FACTORS FOR MANUAL
ASPIRATION MODE,1-12
2 CLEANING PROCEDURES,2-1
2.1 OVERVIEW,2-1
When to Zap and Bleach Apertures,2-1
When to Unclog Apertures,2-1
When to Bleach RBC Internal Electrodes,2-1
When to Clean an Aperture Bath,2-1
When to Clean the Waste Chamber or Vacuum Isolator Chamber,2-1
When to Drain the Overflow Chamber,2-1
When to Clean Air Filters,2-2
When to Clean the BSV and the BSV Drip Plate,2-2
2.2 CLEANING AND REPLACING AIR FILTERS,2-2
Procedure,2-2
For Analyzer,2-3
For Power Supply,2-3
2.3 BLEACHING APERTURES,2-4
Summary,2-4
A Prepare Bleach Solution,2-5
B Put Bleach Solution in WBC Bath,2-5
CUse F05 and Activate Solenoid 12 to Bleach the Hgb Cuvette,2-6
D Refill the WBC Bath with Bleach,2-6
E Put Bleach Solution in RBC Bath,2-7
F Perform F11, Extended Prime. If Necessary, Add More Bleach Solution to the WBC Bath
to
Keep the Liquid Above the Apertures,2-7
G Disconnect the Wash Bottle from WBC Bath,2-8
H Leave the Bleach Solution in the Baths ,2-8
I Go to the Numeric Keypad,2-8
J Press ,2-8
K Perform F12, Extended Clear,2-8
L Press ,2-8
M Fill the Baths with Cleaning Agent and Drain,2-8
N. Press .,2-9
O. Press .,2-9
P Prime the System by Cycling Whole Blood.,2-9
2.4 CLEANING AN APERTURE BATH,2-9
Summary,2-9
Procedure,2-10
A Press until the Aperture Baths Drain Completely into the Waste Chamber,2-10
B Press ,2-10
C Unhook Springs and Pull Out Bath,2-10
D Use Distilled Water to Clean the Bath,2-11

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CONTENTS
E Moisten O-Rings and Return Bath,2-12
F Check for Leaks and Bubbles,2-12
G Close the Compartment Door Beneath the Baths and Aperture Compartment,2-13
H Prime the System by Cycling Whole Blood.,2-13
2.5 CLEARING CLOGGED APERTURES,2-13
2.6 ZAPPING APERTURES,2-14
2.7 CLEANING BLOOD SAMPLING VALVE (OUTSIDE SECTIONS),2-15
Procedure,2-15
2.8 CLEANING BLOOD SAMPLING VALVE (REMOVE AND CLEAN INSIDE),2-16
Summary,2-16
A Prepare Instrument for Removal of BSV,2-16
B Remove BSV,2-17
C Clean BSV,2-19
D Return BSV to its Original Location,2-20
E Clean Up Area Around BSV,2-21
F Verify BSV Functions Properly,2-21
2.9 FLUSHING SYSTEM,2-22
2.10 CLEANING BLOOD SAMPLING VALVE DRIP PLATE,2-23
2.11 CLEARING CLOGGED FLOW CELL,2-24
2.12 DRAINING OVERFLOW CHAMBER,2-25
2.13 CLEANING MANUAL ASPIRATION PROBE WIPE,2-26
2.14 BLEACHING RBC INTERNAL ELECTRODES,2-26
Summary,2-26
Procedure,2-27
A Prepare 50 mL of Bleach Solution in a Wash Bottle,2-27
B Put the Bleach Solution in the WBC Bath,2-28
C Put the Bleach Solution in the RBC Bath,2-28
D Perform Extended Prime,2-29
E Disconnect Sweep-Flow Lines,2-29
F Draw Bleach into RBC Aperture and Drain,2-30
G Draw Cleaning Agent into RBC Apertures and Drain,2-31
H Disconnect Tubing and Reconnect Sweep-flow Lines,2-31
I Perform Extended Clear,2-31
J Press ,2-31
K Press ,2-31
L Prime the System by Cycling Whole Blood in Automatic Mode.,2-31
2.15 CLEANING THE ROCKER BED BELT,2-32
2.16 USING HANDHELD SCANNER,2-33
Cleaning the Scan Window (Welch Allyn®IT 3800),2-34

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2.17 ADVANCED BAR-CODE READER,2-34
Positioning the Advanced Bar-Code Reader,2-36
Up - Service Position ,2-36
Down - Read Position ,2-36
2.18 CLEANING VACUUM ISOLATOR CHAMBER (VIC),2-37
Summary,2-37
A Prepare VIC for Cleaning,2-37
B Bleach VIC,2-38
C Return VIC to Working Order,2-38
D Shut Down Your System,2-38
E Start Up Your System,2-38
2.19 CLEANING WASTE CHAMBER,2-39
Summary,2-39
A Prepare Waste Chamber,2-39
B Bleach Waste Chamber,2-41
C Return Waste Chamber to Working Order,2-41
2.20 CLEANING THE SHEAR VALVE,2-41
2.21 CLEANING THE STRIPPER PLATE,2-44
3 REPLACEMENT PROCEDURES,3-1
3.1 REPLACING AIR FILTERS,3-1
Procedure,3-1
For Analyzer,3-1
For Power Supply,3-1
3.2 REPLACING AN APERTURE BATH,3-2
Summary,3-2
Procedure,3-3
A Press Until the Aperture Baths Drain Completely into the Waste Chamber,3-3
B Press ,3-3
C Turn Off the Main Breaker on the Power Supply,3-3
D Disconnect the Primary Power Cord,3-3
E Unhook the Bath Springs and Gently Pull the Bath Out of the Instrument,3-4
F Label and Disconnect the Tubing Attached to Left Side of the Aperture Bath,3-4
G Replace the O-Rings on the Aperture Block if You Have Spare O-rings Available,3-4
H Replace the Aperture Bath and Reconnect it,3-5
I Reconnect the Primary Power Cord,3-5
J Turn on the Main Breaker on the Power Supply,3-5
K Check for Leaks and Air Bubbles,3-5
L Close the Bath Compartment,3-6
M Press ,3-6
N Run a Control in CBC Test Mode,3-6
3.3 REPLACING CASSETTE LABELS,3-6
3.4 REPLACING CASSETTE SPRING CLIP,3-7

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CONTENTS
3.5 REPLACING CHECK VALVES,3-7
3.6 REPLACING CHOKES,3-8
3.7 REPLACING FUSES,3-8
3.8 REPLACING NEEDLE,3-9
Summary,3-9
Procedure,3-9
A Prepare Instrument for Needle Replacement,3-9
B Attach Safety Clip and Remove Needle Cartridge,3-10
C Clean Needle Assembly Area,3-10
D Remove Needle Tubing,3-11
E Attach Tubing to New Needle,3-12
F Return Needle to Its Original Location,3-13
G Verify Control Recovery,3-13
3.9 REPLACING APERTURE O-RINGS,3-13
Procedure,3-14
A Press Until the Aperture Baths Drain Completely into the Waste Chamber,3-14
B Press ,3-14
C Unhook the Bath Springs and Gently Pull the Bath Out of the Instrument,3-14
D If Replacing the O-Rings on the RBC Bath, Disconnect the Sweep-flow Tubing,3-16
E Unscrew the White Fitting Beneath the Housing,3-16
F Pull the Block Out,3-16
G Replace Defective O-Rings,3-17
H Return Aperture Block to Its Original Location,3-17
I Repeat Steps D through H for the Remaining Aperture Blocks,3-18
J Return the Bath and Reconnect It,3-18
K Check for Leaks and Air Bubbles,3-18
L Close the Bath Compartment,3-19
M Run a Control,3-19
N Review the Control Results,3-19
Removing Panel Covers,3-19
Replacing Pickup Tube Assembly,3-19
Reagent Pickup Tube Replacement,3-19
Waste Pickup Tube Replacement:,3-20
3.10 REPLACING PINCH VALVES,3-20
Summary,3-20
Procedure,3-21
A Go to the Numeric Keypad.,3-21
B Ensure the tubing you want to replace does not contain reagent prior to replacing
It.,3-21
C Ensure you have the same size and type of pinch valve as the one you want to
replace.,3-21
D For reference, make a drawing of the path the tubing takes through the nonfunctioning
valve.,3-21
E Remove the Pinch Valve Tubing and Pinch Valve,3-22
F Install the Tubing and New Pinch Valve,3-23
G Check the drawing you made in step 3. Make sure the tubing is properly routed.,3-24

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CONTENTS
H Press ,3-24
I If necessary, prime the reagent associated with the tubing.,3-24
J Run a sample. Make sure that the new valve is correctly connected and there are no
leaks.,3-24
3.11 REPLACING REAGENT CONTAINERS,3-24
Recording Information in Your Logbook,3-25
3.12 REPLACING DILUENT WHEN USING DUAL PICK-UP TUBES,3-25
3.13 REPLACING TUBING,3-26
3.14 REPLACING WASTE CONTAINER,3-27
4 ADJUSTMENT PROCEDURES,4-1
4.1 CHANGING TEST MODE,4-1
4.2 CHANGING ANALYZER ALARM SETTINGS,4-1
4.3 TURN PNEUMATICS OFF,4-1
4.4 ADJUSTING HGB LAMP VOLTAGE,4-2
4.5 SETTING UP BAR-CODE CONFIGURATION,4-2
Saving/Restoring Configuration Information,4-3
Saving Configuration Information,4-3
Restoring Configuration Information,4-3
Archive Complete Database,4-4
4.6 ENABLING/DISABLING BLOOD DETECTOR,4-4
4.7 SETTING UP ANALYZER DATE FORMAT,4-4
4.8 SETTING UP ANALYZER DATE AND TIME,4-5
4.9 SETTING UP FLOW CELL AUTOSTOP,4-5
4.10 ADJUSTING TUBE DETECTOR,4-6
4.11 CONFIGURING HANDHELD SCANNER,4-8
Configuring the Handheld Scanner,4-8
Keyboard Language Code Settings,4-9
Reset to Coulter Corporation Defaults,4-9
Code 39,4-9
Interleaved 2-of-5,4-10
Codabar,4-10
5 TROUBLESHOOTING,5-1
5.1 OVERVIEW,5-1
Data Review,5-1
Specimen-Related Problems,5-1
Instrument Problems,5-1

PN 773023AC xi
CONTENTS
Workstation Troubleshooting,5-1
Electronic Troubleshooting,5-1
Indicators,5-1
Electronic Tests,5-2
Correcting Electronic Problems,5-2
Pneumatic/Hydraulic Troubleshooting,5-2
Pinched Tubing,5-2
Plug,5-2
Leak,5-2
Defective Components,5-2
Correcting Pneumatic/Hydraulic Problems,5-2
Reagent Troubleshooting,5-3
Correcting Reagent Problems,5-3
5.2 DILUTER FUNCTIONS,5-3
Alphabetically by Function,5-3
Drain the Overflow Chamber,5-4
Drain the Vacuum Isolator Chamber,5-4
Drain the Waste Chamber,5-5
Activating and Keeping Solenoid Active,5-5
Draining The Vacuum Overflow Tank,5-6
Verifying Backwash Tank Operation,5-6
F27 - Dispensing CBC Lytic Reagent,5-7
Cleaning Apertures,5-7
Running Latex Control—Diff and Retic,5-7
Running Latex Control—Diff,5-9
Running Latex Control—Retic,5-10
F23 - Dispensing Diff Lytic Reagent Low Volume,5-12
F24 - Dispensing Diff Lytic Reagent High Volume,5-12
F21 - Dispensing Diff Preservative Low Volume,5-12
F22 - Dispensing Diff Preservative High Volume,5-13
Dispensing Diluent,5-13
Dispensing Diluent into RBC Bath,5-14
Dispensing Diluent into WBC Bath,5-14
Performing Extended Prime,5-15
Performing Extended Clear,5-15
Clearing Flow Cell (Level 1),5-16
Clearing Flow Cell (Level 2),5-16
Clearing Flow Cell (Level 3),5-17
Performing Keypad Key Test,5-17
Priming CBC Lytic Reagent,5-18
Priming Cleaning Agent,5-18
Priming Diff Lytic Reagent,5-19
Priming Diff Preservative,5-19
Priming Diluent,5-20
Priming Retic Clearing Solution,5-20
Priming Retic Stain Solution,5-21
Priming Sweep Flow,5-21
Performing Probe Wipe Test,5-22
Performing Flow Cell Purge,5-22

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CONTENTS
Forcing Mixing Bubbles into Baths,5-23
Releasing Blood Sampling Valve (BSV),5-23
Refilling the Reagent Reservoirs,5-24
Dispensing Retic Stain,5-24
Dispensing Retic Clearing Solution,5-25
Performing Sheath Tank Check,5-25
Freeing Solenoids,5-25
Enabling/Disabling Access to Cycle Control,5-26
Perform an Initial Prime of Diluter,5-26
Monitoring Low Vacuum,5-27
Advancing the Tube,5-27
Zap Apertures Function,5-27
5.3 SOLENOID FUNCTIONS,5-29
5.4 RUNNING BACKGROUND TEST,5-32
5.5 RUNNING PRECISION TEST,5-33
5.6 RUNNING RAMP TEST,5-33
5.7 READING HGB VOLTAGE FOR LAST CYCLE,5-33
5.8 CHECKING THE NEEDLE,5-34
5.9 CHECKING PINCH VALVES,5-34
5.10 RESEATING AN ANALYZER CARD,5-35
5.11 ANALYZER RESET BUTTON,5-38
5.12 WORKING WITH ELECTRONIC HISTORY LOGBOOKS,5-38
Adding Comments to an Electronic Logbook,5-39
Adding Your Laboratory Procedures,5-39
5.13 OPENING THE DILUTER LOWER COVER,5-40
5.14 CLOSING THE DILUTER LOWER COVER,5-41
5.15 AUTOSTOP,5-41
AutoStop Options,5-42
5.16 COMMUNICATION HAS BEEN LOST BETWEEN THE ANALYTICAL STATION AND
WORKSTATION,5-42
5.17 FLUSHING LOW VACUUM LINES,5-43
5.18 FLUSHING THE BACKWASH TANK VACUUM SUPPLY LINE,5-49
6 ERROR MESSAGES,6-1
6.1 SYSTEM AND WORKSTATION MESSAGES,6-1
System Alarms,6-1
About Table 6.2, System messages,6-1

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CONTENTS
Icons and Traffic Lights Displayed on the Workstation with System Messages,6-2
About, Workstation Messages,6-2
Icons and Traffic Lights Displayed on the Workstation with Workstation Only Messages,6-3
6.2 DILUTER ERRORS,6-4
6.3 NON-DILUTER ERRORS,6-5
6.4 ANALYTICAL STATION MESSAGES,6-7
6.5 WORKSTATION MESSAGES,6-49
INDEX,INDEX-1
TRADEMARKS,1

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CONTENTS

PN 773023AC xv
INTRODUCTION
This introductory section contains the following topics:
r How to use your COULTER®LH 780 System hard-copy manuals
r About this manual
r Online Help System
r Conventions.
HOW TO USE YOUR COULTER®LH 780 SYSTEM HARD-COPY MANUALS
Use the Reference manual for in-depth information about what the instrument does, the
methods it uses, its specifications, and information on installation, safety and software
options. The Reference manual for the LH 780 System is included in the online Help system;
it is available in hard copy by request.
Use the Special Procedures and Troubleshooting manual to run calibration; to clean, replace
or adjust a component on the instrument; and for troubleshooting the instrument. This
document is made up of procedures from the online Help system; it is available in hard copy
by request.
Use the Operator’s Guide for the day-to-day operation of your instrument. This document is
made up of procedures from the online Help system; it includes Startup, running controls and
samples, reviewing data, Shutdown, and the software on the Analyzer and the Workstation.
This document is available in hard copy by request.
Use the SlideMaker Operator’s Guide for in-depth information about what the SlideMaker
does, the methods it uses, its specifications, and information on installation, safety and
software, as well as day-to-day operating and troubleshooting your SlideMaker. This
document is made up of procedures from the online Help system; it is available in hard copy
by request.
Use the SlideStainer Operator’s Guide for the day-to-day operating and troubleshooting of
your SlideStainer. This document is made up of procedures from the online Help system; it
includes in-depth information about what the SlideStainer does, the methods it uses, its
specifications, and information on installation, safety and software. This document is
available in hard copy by request.
Use the Host Transmission Specification to find the information needed to program the
transmission interface between the LH 700 SERIES System and your laboratory’s host
computer. This document is available in hard copy by request.
See the Documentation page on the back cover of this manual for the contents of each
manual. It can help you to determine quickly in which manual the information you need is
located.
ABOUT THIS MANUAL
Your LH 780 Special Procedures and Troubleshooting Guide is a source of information for the
day-to-day operation of your instrument. This information is organized as follows:

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INTRODUCTION
ONLINE HELP SYSTEM
r Chapter 1, Calibration
Provides procedures for reproducibility, carryover, calibration with S-CAL calibrator and
calibration with whole blood.
r Chapter 2, Cleaning Procedures
Provides procedures for cleaning parts of the system.
r Chapter 3, Replacement Procedures
Provides procedures for replacing parts of the system.
r Chapter 4, Adjustment Procedures
Provides procedures for adjusting parts of the system.
r Chapter 5, Troubleshooting
Provides an overview of troubleshooting concepts, describes the Numeric keypad
functions, lists the solenoids, describes how to reset the Analyzer and the Workstation,
describes the Workstation logs, and lists the error messages.
r Chapter 6, Error Messages
Provides a list of all the error messages that appear on the system.
r Index, hard copy only.
ONLINE HELP SYSTEM
The LH Workstation has a comprehensive Online Help System, which includes reference
information, all operating, maintenance and troubleshooting procedures. On the LH
Workstation, select to access Help.
CONVENTIONS
This document uses the following conventions:
indicates a key on the Numeric keypad.
indicates a key on the LH Workstation keyboard.
is the icon for Patient results on the LH Workstation.
is the icon for the Printer on the LH Workstation.

PN 773023AC 1-1
1
1CALIBRATION
1.1 OVERVIEW
Calibration fine-tunes the LH 700 Series System so it provides the most accurate results
possible.
Your laboratory is responsible for the final calibration of the CBC parameters and for
recording the calibration factors. Beckman Coulter recommends S-CAL®calibrator, or an
exact equivalent, as an acceptable alternative to whole-blood calibration.
In the normal process of tracking data for an extended period of time, your laboratory can
make a specific decision to recalibrate a given parameter. Never adjust to a specific value for
an individual sample.
For best performance, calibrate all the CBC parameters. The WBC differential and Retic
parameters are calibrated at the factory; they do not require calibration in the laboratory.
When to Calibrate
You should calibrate your instrument:
r At installation
r After the replacement of any component that involves dilution characteristics (such as
the BSV) or the primary measurements (such as the apertures)
r When advised to do so by your Beckman Coulter Representative.
You should verify the calibration of your instrument:
r As dictated by your laboratory procedures, local or national regulations
r When controls begin to show evidence of unusual trends
r When controls exceed the manufacturer's defined acceptable limits.
r If the average ambient room temperature changes more than 10°F from the calibrating
temperature.
1.2 PERFORMING REPRODUCIBILITY CHECK
Sample Requirements
For reproducibility studies, ensure the patient for the sample:
r is receiving no medication
r has normal hematologic parameters, with a WBC count of 10,000 ±1,000.
r has normal erythrocyte, leukocyte and platelet morphology and, if you want to check the
Diff parameters, with Diff values:
Neutrophils 40 to 72%
Lymphocytes 17 to 45%
Monocytes 4 to 12%
Eosinophils 0 to 10%
Basophils 0 to 1%

1.3–2 PN 773023AC (<$condtag[B,C,D,E,F,G,H,J,K,M,N,P,Q,R,S,T,U,V,W,X,Y,Z,A]>)
PN 773023AC
1-2
CALIBRATION
REVIEWING REPRODUCIBILITY RESULTS
1. Ensure you have enough normal whole blood from a single donor for a minimum of 11
and a maximum of 31 cycles.
2. Ensure pneumatics are on.
3. Ensure the blood detector is enabled.
4. Ensure the number of aspirations per tube is set to 1.
5. Select the desired mode of operation.
6. Select REPRODUCIBILITY as the process type on the Command Center.
7. If necessary, select on the Reproducibility window to clear out the values that
appear on the results table.
Note: If you want to view the reproducibility results as the Workstation receives them,
you can access the Reproducibility window (step 14) before proceeding. The background
of the Reproducibility window changes to the same background as the Command Center
when you change the process type to REPRODUCIBILITY.
8. Allocate approximately 1 ml of the well mixed normal whole blood sample into one
tube.
9. Cycle one sample of normal whole blood in Automatic aspiration mode.
10. Set the number of aspirations per tube to 5.
11. Separate the well-mixed normal whole-blood sample into two tubes. (Additional tubes
are necessary for reproducibility greater than n = 10.)
12. Place the tubes into consecutive positions in a cassette and place the cassette in the
loading bay. The system automatically begins processing the cassette. It pierces,
aspirates, and analyzes the samples.
13. Select on the Command Center to display the Quality Assurance application.
14. Select to display the Reproducibility window.
15. Review the results table and statistics table on the window. Use the scroll bars to review
results that do not appear on the window.
16. Verify that the CV (Coefficient of Variation) does not exceed the established
Reproducibility Limits. If limit is exceeded, repeat steps 10 through 12 under Performing
Reproducibility Check, until at least 11 but not more than 31 whole blood samples have
been analyzed. You may stop the analysis at any point between 11 and 31 runs if the
reproducibility established limits have been met.
17. Upon completion of reproducibility, select AUTO ANALYSIS as the process type on the
command center for continuing with sample analysis.
1.3 REVIEWING REPRODUCIBILITY RESULTS
Note: To perform this task, you must log on with a user name that was set up as an Advanced
Operator or Lab Administrator. If you need to access this function, contact your laboratory
administrator.
CAUTION Needle damage can occur if you pierce a specimen tube more than five times. Do not pierce a
specimen tube more than five times.

PN 773023AC 1-3
CALIBRATION
PERFORMING CARRYOVER CHECK 1
1. Select on the Command Center to display the Quality Assurance application.
2. Select to display the Reproducibility window.
3. Review the results table and statistics table on the window. Use the scroll bars to review
results that do not appear on the window.
4. Verify that the CV (Coefficient of Variation) doe not exceed the established limits. If
limit is exceeded, repeat steps 8 through 13 under Performing Reproducibility Check,
until at least 11 but not more than 31 whole blood samples have been analyzed. You may
stop the analysis at any point between 11 and 31 runs if the reproducibility established
limits have been met.
Reproducibility Limits for CBC
1.4 PERFORMING CARRYOVER CHECK
1. Ensure pneumatics are on.
2. Ensure the blood detector is disabled.
3. Ensure the mode of operation is set to CBC. You can perform carryover check using
other operating modes, however the procedure will take longer and use excess reagents.
4. Ensure the number of aspirations per tube is set to 1.
5. Select CARRYOVER as the process type on the Command Center.
Note: If you want to view the carryover results as the Workstation receives them, you
can access the Carryover window (step 13) before proceeding. The background of the
Carryover window changes to the same background as the Command Center when you
change the process type to CARRYOVER.
6. If necessary, select on the Carryover window to clear out the values that appear on
the results table.
7. Obtain a normal whole-blood specimen.
8. Separate the well-mixed normal whole-blood specimen into two 5 mL tubes.
9. Place the tubes into consecutive positions in a cassette.
10. Dispense 2 mL of diluent into three separate 5 mL tubes.
11. Place the three diluent tubes in the same cassette. Place the tubes after the whole-blood
specimens.
Parameter %CV
WBC <= 1.7
RBC <= 0.8
Hgb <= 0.8
MCV <= 0.8
Plt <= 3.3
MPV <= 2.2

1.5–4 PN 773023AC (<$condtag[B,C,D,E,F,G,H,J,K,M,N,P,Q,R,S,T,U,V,W,X,Y,Z,A]>)
PN 773023AC
1-4
CALIBRATION
REVIEWING CARRYOVER RESULTS
12. Place the cassette in the loading bay. The instrument begins processing the cassette
automatically.
13. Verify the carryover results.
14. Enable the blood detector.
15. On the Command Center, select AUTO ANALYSIS as the process type if you are not
continuing to either Reproducibility or Calibration.
Carryover Formula
The LH 700 Series software calculates the percent carryover by using:
(1st diluent - 3rd diluent) / 2nd sample x 100 = % carryover
1.5 REVIEWING CARRYOVER RESULTS
Note: To perform this task, you must log on with a user name that was set up as an Advanced
Operator or Lab Administrator. If you need to access this function, contact your laboratory
administrator.
1. Select on the Command Center to display the Quality Assurance application.
2. Select to display the Carryover window.
3. Verify that the message CARRYOVER ACCEPTABLE appears on the Carryover window. If
Carryover is unacceptable, the unacceptable values appear flagged. Review the results
table and the limits table.
Note: If negative carryover values persist, and the third diluent is higher than the first
two, check background. If the problem persists, an instrument problem may exist, call
your Beckman Coulter Representative.
4. Select to print the carryover results for your logbook.
1.6 CALIBRATING CBC PARAMETERS WITH S-CAL®CALIBRATOR
Summary
A. Ensure the apertures are clean.
B. Ensure the instrument is functioning properly.
C. Prepare the instrument for calibration.
D. Set up the CBC calibration information at the Workstation.
E. Run S-CAL calibrator.
F. Review results on the Calibration window.
G. Adjust parameters as needed.
H. On the Command Center, select AUTO ANALYSIS as the process type.