Beckman Coulter CytoFLEX SRT User manual

C37808AC
March 2022
Instructions for Use
CytoFLEX SRT
Cell Sorter
For Research Use Only. Not for use in diagnostic procedures.
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

CytoFLEX SRT
Cell Sorter
PN C37808AC (March 2022)
© 2022 Beckman Coulter, Inc.
All Rights Reserved.
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Coulter product and service marks mentioned herein are
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Inc. in the United States and other countries.
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All other trademarks are the property of their respective
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Glossary of Symbols is available at beckman.com/
techdocs (PN C24689).
Original Instructions

C37808AC iii
Revision History
Initial Issue AA, 01/2021
Software Version: CytExpert SRT 1.0
Issue AB, 08/2021
Software Version: CytExpert SRT 1.0
Updates were made to the following sections:
CHAPTER 1, System Overview
Sample Temperature Control
Sorter
Performance Characteristics
CHAPTER 2, Using the CytExpert SRT Software
Acquisition/Sorting Control
Software Menu
Sort Mode Library
Software Settings
CHAPTER 4, Instrument Quality Control and Standardization
Overview
Preparing the QC Sample
Importing Lot-Specific Target Values
Collecting QC Data
Preparing the Standardization Sample
CHAPTER 5, Sorting
Default Amplitude Setting (Optional)
Setting Sample Flow Rate
Setting Up Tube Sorting
Setting Up Plate/Slide Sorting
Calibrating the Sort Collection Device
Manual Side Stream Calibration (Optional)
Side Stream Monitor
Auto Maintain
Viewing Sorting Statistics
Pause Sorting and Resume Sorting
CHAPTER 7, Data Review
Calculating Sample Concentration
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

C37808AC
iv
Revision History
CHAPTER 9, Troubleshooting
Laser Warning Labels
Troubleshooting Table
CHAPTER 11, Replacement/Adjustment Procedures
Replacing the Nozzle
Replacing the Sample Line
Setting Laser Delay
APPENDIX B, Consumables
Consumables
APPENDIX C, Biosafety Cabinet Accessory
Biosafety Cabinet Installation
Biosafety Cabinet Specification
APPENDIX E, Good Practices for Cyber Security
Installing the Operating System Updates
Issue AC, 03/2022
Software Version: CytExpert SRT 1.1
Updates were made to the following sections:
CHAPTER 1, System Overview
Fluidics Cart
Sheath Tank
Wavelength Division Multiplexer (WDM)
CyClone Movement System
System Connections
Sorter
CHAPTER 2, Using the CytExpert SRT Software
Overview
Start Page
Tube Management
Software Menu
Acquisition and Analysis Screen Menu
Account Menu
Log Menu
Backup/Restore Menu
User Management
Role Management
Account Policies
Operation Log
Collection Device Library
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

C37808AC v
Revision History
Software Settings
CHAPTER 3, Daily Startup
Logging Into the Software
System Startup Program
CHAPTER 4, Instrument Quality Control and Standardization
Creating Levey-Jennings Charts
CHAPTER 5, Sorting
Setting Drop Delay Manually (Optional)
Setting the Channel and Label
Creating Plots and Gates
Adjusting the Gain
Setting Collection Conditions
Setting Mixing Speed
Setting Up Violet Side Scatter (VSSC) Channel
Verifying, Selecting, Editing, and Creating Detector Configuration
Setting Up Tube Sorting
Setting Up Plate/Slide Sorting
Adjusting Tube Position (Optional)
CHAPTER 8, Shutting Down the System
Long Term Shutdown
CHAPTER 9, Troubleshooting
Overview
Laser Related Hazards
Troubleshooting Table
Backup and Restore
CHAPTER 10, Cleaning Procedures
Aseptic Clean Program
CHAPTER 11, Replacement/Adjustment Procedures
Removing Trapped Air Bubbles
Replacing the Sample Probe
Resetting the Sheath Tank Scale
APPENDIX D, Instrument Installation
Overview
Power Source
Waste Disposal
CytExpert Software Installation Options
Installing the CytExpert SRT Software
Upgrading and Reinstalling the CytExpert SRT Software
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.