Beckman Coulter CytoFLEX SRT User manual

C37808AC
March 2022
Instructions for Use
CytoFLEX SRT
Cell Sorter
For Research Use Only. Not for use in diagnostic procedures.
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

CytoFLEX SRT
Cell Sorter
PN C37808AC (March 2022)
© 2022 Beckman Coulter, Inc.
All Rights Reserved.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
Windows 7, Windows 8, and Windows 10 are registered
trademarks or trademarks of Microsoft Corporation in the
United States and/or other countries.
All other trademarks are the property of their respective
owners.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckman.com/support/technical
• In the USA and Canada, call us at 1-800-369-0333.
• In Austria, call us at 0810 300484.
• In Germany, call us at 02151 333999.
• In Sweden, call us at [phone].
• In Netherlands, call us at [phone].
• In France, call us at 0825838306 6.
• In the UK, call us at [phone].
• In Ireland, call us at [phone].
• In Italy, call us at [phone].
• In other locales, contact your local Beckman Coulter
Representative.
May be covered by one or more pat.-see
www.beckman.com/patents
Beckman Coulter (UK) Ltd.
Oakley Court
Kingsmead Business Park, London Road
High Wycombe
United Kingdom HP11 1JU
01494 441181
Glossary of Symbols is available at beckman.com/
techdocs (PN C24689).
Original Instructions

C37808AC iii
Revision History
Initial Issue AA, 01/2021
Software Version: CytExpert SRT 1.0
Issue AB, 08/2021
Software Version: CytExpert SRT 1.0
Updates were made to the following sections:
CHAPTER 1, System Overview
Sample Temperature Control
Sorter
Performance Characteristics
CHAPTER 2, Using the CytExpert SRT Software
Acquisition/Sorting Control
Software Menu
Sort Mode Library
Software Settings
CHAPTER 4, Instrument Quality Control and Standardization
Overview
Preparing the QC Sample
Importing Lot-Specific Target Values
Collecting QC Data
Preparing the Standardization Sample
CHAPTER 5, Sorting
Default Amplitude Setting (Optional)
Setting Sample Flow Rate
Setting Up Tube Sorting
Setting Up Plate/Slide Sorting
Calibrating the Sort Collection Device
Manual Side Stream Calibration (Optional)
Side Stream Monitor
Auto Maintain
Viewing Sorting Statistics
Pause Sorting and Resume Sorting
CHAPTER 7, Data Review
Calculating Sample Concentration
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

C37808AC
iv
Revision History
CHAPTER 9, Troubleshooting
Laser Warning Labels
Troubleshooting Table
CHAPTER 11, Replacement/Adjustment Procedures
Replacing the Nozzle
Replacing the Sample Line
Setting Laser Delay
APPENDIX B, Consumables
Consumables
APPENDIX C, Biosafety Cabinet Accessory
Biosafety Cabinet Installation
Biosafety Cabinet Specification
APPENDIX E, Good Practices for Cyber Security
Installing the Operating System Updates
Issue AC, 03/2022
Software Version: CytExpert SRT 1.1
Updates were made to the following sections:
CHAPTER 1, System Overview
Fluidics Cart
Sheath Tank
Wavelength Division Multiplexer (WDM)
CyClone Movement System
System Connections
Sorter
CHAPTER 2, Using the CytExpert SRT Software
Overview
Start Page
Tube Management
Software Menu
Acquisition and Analysis Screen Menu
Account Menu
Log Menu
Backup/Restore Menu
User Management
Role Management
Account Policies
Operation Log
Collection Device Library
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

C37808AC v
Revision History
Software Settings
CHAPTER 3, Daily Startup
Logging Into the Software
System Startup Program
CHAPTER 4, Instrument Quality Control and Standardization
Creating Levey-Jennings Charts
CHAPTER 5, Sorting
Setting Drop Delay Manually (Optional)
Setting the Channel and Label
Creating Plots and Gates
Adjusting the Gain
Setting Collection Conditions
Setting Mixing Speed
Setting Up Violet Side Scatter (VSSC) Channel
Verifying, Selecting, Editing, and Creating Detector Configuration
Setting Up Tube Sorting
Setting Up Plate/Slide Sorting
Adjusting Tube Position (Optional)
CHAPTER 8, Shutting Down the System
Long Term Shutdown
CHAPTER 9, Troubleshooting
Overview
Laser Related Hazards
Troubleshooting Table
Backup and Restore
CHAPTER 10, Cleaning Procedures
Aseptic Clean Program
CHAPTER 11, Replacement/Adjustment Procedures
Removing Trapped Air Bubbles
Replacing the Sample Probe
Resetting the Sheath Tank Scale
APPENDIX D, Instrument Installation
Overview
Power Source
Waste Disposal
CytExpert Software Installation Options
Installing the CytExpert SRT Software
Upgrading and Reinstalling the CytExpert SRT Software
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

C37808AC
vi
Revision History
APPENDIX E, Good Practices for Cyber Security
Enabling Audit Object Access
Viewing Windows Security Logs
Note: Changes that are part of the most recent revisions are indicated in text by a bar in the margin of the
amended page.
This document applies to the latest software listed and higher versions. When a subsequentsoftware version affects the
information in this document, a new issue will be released to the Beckman Coulter Web site. For labeling updates, go to
www.beckman.com and download the latest version of the manual or system help for your instrument.

C37808AC vii
Safety Notices
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact us.
Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards
such as the use of barrier protection. This may include, but it is not limited to, protective eyewear,
gloves, and suitable laboratory attire when operating or maintaining this or any other automated
laboratory analyzer.
This manual assumes that users have basic knowledge of the Windows operating system, as well as
experience working with laboratory testing technology. Users are invited to consult the
appropriate documentation for such information.
Alerts for Warning, and Caution
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices.

C37808AC
viii
Safety Notices
Safety Precautions
Safety Precautions
WARNING
Risk of operator injury if:
• All doors, covers and panels are not closed and secured in place prior to and
during instrument operation.
• The integrity of safety interlocks and sensors is compromised.
• Instrument alarms and error messages are not acknowledged and acted upon.
•Youcontactmovingparts.
• You mishandle broken parts.
• Doors, covers, and panels are not opened, closed, removed and/or replaced
with care.
• Improper tools are used for troubleshooting.
To avoid injury:
• Keep doors, covers and panels closed and secured in place while the
instrument is in use.
• Take full advantage of the safety features of the instrument. Do not defeat
safety interlocks and sensors.
• Acknowledge and act upon instrument alarms and error messages.
• Keep away from moving parts.
• Report any broken parts to your Beckman Coulter Representative.
• Open/remove and close/replace doors, covers and panels with care.
• Use the proper tools when troubleshooting.
CAUTION
System integrity could be compromised and operational failures could occur if:
• This equipment is used in a manner other than specified. Operate the
instrument as instructed in the Product Manuals.
• You introduce software that is not authorized by Beckman Coulter into your
computer. Only operate your system's computer with software authorized by
Beckman Coulter.
• You install software that is not an original copyrighted version. Only use
software that is an original copyrighted version to prevent virus
contamination.

C37808AC ix
Safety Notices
Instrument Safety Precautions
CAUTION
If you purchased this product from anyone other than Beckman Coulter or an
authorized Beckman Coulter distributor, and it is not presently under a Beckman
Coulter service maintenance agreement, Beckman Coulter cannot guarantee that
the product is fitted with the most current mandatory engineering revisions or
that you will receive the most current information bulletins concerning the
product. If you purchased this product from a third party and would like further
information concerning this topic, contact us.
CAUTION
Risk of instrument damage. This device is intended for indoor use only. To avoid
device damage, do not install the instrument outdoors.
WARNING
Risk of personal injury. Safety protection can be impaired if used in a manner not
specified by the manufacturer. To avoid personal injury, use the instrument
according to the manufacturer's instructions only.
Instrument Safety Precautions
The instrument has been engineered with safety as one of its primary features. Safety of the
operator, field service personnel, bystanders, and valuable samples, is paramount to Beckman
Coulter’s commitment to high performance design and engineering.
This section explains some general safety and hazard symbols and necessary precautions operators
of the instrument should follow during operation. Engineering controls have been put in place to
protect the operator, and deliberate misuse of the instrument or its instructions may result in
unintentional harm. Please follow all safety and hazard instructions as directed.
General Safety
To protect the health, environment, and safety of instrument sites and their users, the following
information should be reviewed by all operators.
• The instruments are intended for Professional Use Only. All operators should be trained on the
proper use and limitations of the instrument prior to its operation.
• Be aware that the Sort Chamber, Interrogation Chamber, and cabinet doors on the instrument
present possible pinch points. While the doors are light-weight and do not have sharp edges,
care should be taken when opening and closing doors.
• The view screen on the Biosafety Cabinet can be moved manually up and down thereby creating
a possible pinch point. Position hands appropriately when moving the view screen on the
Biosafety Cabinet.

C37808AC
x
Safety Notices
Instrument Safety Precautions
• The sample station has electronically controlled moving parts. Do not insert your hand in the
sample chamber when it is moving.
• Wear appropriately sized gloves providing good manual dexterity to reduce the likelihood of
skin pinches and abrasions.
• Use proper lifting techniques or seek assistance when handling the BioSafety Cabinet, or full
tanks. Take care to reduce the likelihood of back injury.
• Protect the skin and eyes whenever handling chemicals of any kind, regardless of how benign
they may appear.
• Check with the site safety officer for correct disposal of waste products and for spill clean-up
protocols.
• The user should rest appropriately to avoid strain due to repetitive use, awkward movements,
or sitting too long.
Biohazard Safety
Use universal precautions when working with pathogenic materials. Means must be available to
decontaminate the instrument and to dispose of biohazardous waste.
IMPORTANT If any hazardous organism, material, or agent is used in the instrument, the site operator or
Principal Investigator is responsible for informing Beckman Coulter in writing of those hazards before
receiving service or repair. This includes a list of all pathogenic cell lines, hazardous reagents, radioactive
material, or agents with a BSL Level II or higher. This information will be kept confidential and will be used
to inform Beckman Coulter Field Service Representatives of any hazards prior to visiting any instrument
site. Failure to report this information may delay service on an instrument. Safety of the user as well as
safety of Beckman Coulter employees is of overriding importance. Proper decontamination procedures
must be followed for all applicable returned parts.
• Gloves, a laboratory coat, and eye protection should be worn whenever handling samples
including insertion and removal of sample tubes from the sample station.
• If the system loses vacuum or the waste tube becomes clogged, waste fluid could spill into the
sort chamber. Immediately turn off sheath and sample flow, wear proper personal protective
equipment and attend to the spill.
• Waste fluid may contain hazardous levels of biological and chemical contamination. Gloves, a
laboratory coat and eye protection should be worn whenever exposure to waste fluid exists.
• To ensure inactivation of biological organisms in the waste container, an appropriate type and
quantity of an EPA registered disinfectant should be placed in the tank initially upon use, and
every time the waste container is emptied and reinstalled.
• The Aerosol Containment Shield, also known as the Sort Chamber door, is part of a passive
aerosol containment assembly that isolates the contents of a sort from the rest of the
instrument, the operator, and the laboratory. When closed, the door limits movement of air
into and out of the Sort Chamber. It is optional to purchase an Aerosol Evacuation system for
additional protection from aerosols. See Aerosol Evacuation System.
NOTE Use of the Aerosol Evacuation system precludes the use of Biosafety Cabinet, or vise versa.
• A BSL-2, Level A2 Biosafety Cabinet is available for purchase as an optional system accessory.
Refer to APPENDIX C, Biosafety Cabinet Accessory.

C37808AC xi
Safety Notices
Instrument Safety Precautions
For additional information on laboratory biosafety, please review the U.S. Department of Health
and Human Services, Centers for Disease Control document, Biosafety in Microbiological and
Biomedical Laboratories. Contact the safety officer at your site and discuss proper waste disposal
precautions and practices. Consult the Original Equipment Manufacturer (OEM) manuals for the
Biosafety Cabinet and the Aerosol Evacuation System for additional information.
Electrical Safety
The instrument conforms to international regulations encompassing the accessibility of high
voltages by the user (IEC 61010-1) and exposure to laser emission: IEC 60825-1:2014 Safety of Laser
Products - Part 1: Equipment Classification and Requirements; 21 CFR 1040 FDA/CDRH Laser
Product Performance Standard. Please familiarize yourself with the following features of the
instrument and their corresponding potential hazards.
Safety Interlocks
Under normal operating conditions, the instrument protects the user from exposure to high
voltages and is considered a Class 1 laser product. The instrument is equipped with four safety
interlocks designed to protect the operator from inadvertent exposure to high voltage, laser
radiation, and pinch hazard.
• When the Sort Protection door opens, the safety interlock disables the voltage to the deflection
plates.
• When the optical bench cover is opened, all the lasers turn off.
• When the sample station door is opened, the movement of the sample chamber stops and the
system LED indication turns red.
DO NOT attempt to defeat these interlocks except when this document specifically instructs you to
do so. Ensure that you have the proper laser safety training prior to defeating safety interlocks.
Stream Charge
When the sheath stream is charged and individual droplets are formed, the droplets retain the
charge present on the stream.
• Do not defeat the safety interlock and insert any object into the charged stream.
Drop Drive Voltage
This ranges from 50-250 Vac and is used to drive the piezoelectric crystal mounted in the nozzle.
The frequency can be set either by Auto Droplet Calibration or by the operator.

C37808AC
xii
Safety Notices
Instrument Safety Precautions
Deflection Plates
CAUTION
Risk of personal injury. Do not touch the charged plates when power is applied.
The range of voltage applied to these plates is ±3800 ~ ±4200 Vdc. This high voltage is present only
when the plate voltage is turned on and the interlock is closed. High voltage is accessible only if the
interlock is defeated, and only if the operator inserts an object between the charged plates. Once
high voltage is enabled by the operator, it is constant until changed by the operator.
Deflection Plate Arcing
Arcing may occur due to buildup of sheath solution on the deflection plates. If arcing occurs, refer
to deflection plate arcing in CHAPTER 9, Troubleshooting.
Laser Power Supplies
Laser power supplies have dangerous amounts of energy and could be a hazard to the operator.
Contact us if laser power supplies require service.
Electromagnetic Compatibility
This device complies with the emissions and immunity requirements as specified in the EN/IEC
61326 series of Product Family Standards for a “basic electromagnetic environment.” Such
equipment is supplied directly at low voltage from public mains network. This equipment is not
intended for residential use.

C37808AC xiii
Safety Notices
Symbol Explanations
CAUTION
This device generates, uses and can radiate un-intentional radio-frequency (RF)
energy. If this device is not installed and operated correctly, this RF energy can
cause interference with other equipment. It is the responsibility of the end user to
ensure that a compatible electromagnetic environment for the device can be
maintained in order that the device will operate as intended.
In addition, other equipment can radiate RF energy to which this device is
sensitive. If one suspects interference between this device and other equipment,
Beckman Coulter recommends the following actions to correct the interference:
1. Evaluate the electromagnetic environment before installation/operation of
this device.
2. Do not operate this device close to sources of strong electromagnetic
radiation (e.g. unshielded intentional RF sources), as these can interfere with
proper operation. Examples of unshielded intentional radiators would be
hand-held radio transmitters, cordless phones and cellular phones.
3. Do not place this device near medical electrical equipment that can be
susceptible to malfunctions caused by close-proximity to electromagnetic
fields.
4. This device has been designed and tested to CISPR 11, Class A emission limits.
In a domestic environment, this device may cause radio interference, in which
case, you may need to take measures to mitigate the interference.
Symbol Explanations
Safety symbols alert you to potentially dangerous conditions. The symbol applies to specific
procedures and appears as needed throughout this manual.
Symbol Warning Condition Action
Biohazard Symbol Consider all materials (specimens, reagents, controls,
and monoclonal antibodies) and areas these materials
come into contact with as being potentially infectious.
Wear appropriate barrier protection and follow safe
laboratory procedures when handling any material in
the laboratory.
Caution To indicate that caution is necessary when operating
the device or control close to where the symbol is
placed, or to indicate that the current situation needs
operator awareness or operator action in order to
avoid undesirable consequences.
Caution, Risk of Electric
Shock
To indicate hazards arising from dangerous voltages.

C37808AC
xiv
Safety Notices
Symbol Explanations
Pinch To warn of a closing motion of mechanical parts of
equipment.
Recycling Symbol
WEEE Wheeled Bin
Symbol
The symbol of a crossed-out wheeled bin on the
product is required in accordance with the Waste
Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of
this marking on the product indicates:
1. that the device was put on the European Market
after August 13, 2005 and
2. that the device is not to be disposed via the
municipal waste collection system of any member
state of the European Union.
For products under the requirement of WEEE
directive, please contact your dealer or local
Beckman Coulter office for the proper
decontamination information and take-back
program which will facilitate the proper
collection, treatment, recovery, recycling, and
safe disposal of device.
For the Japan market:
This system is considered an industrial waste, subject
to special controls for infectious waste. Before disposal
of the system, refer to the Waste Disposal and Public
Cleaning Law for compliance procedures.
Laser Radiation The laser radiation symbol indicates that there
can be laser light radiation in the area. Take
precautions to prevent exposure.
RoHS Caution Symbol This label indicates that the electronic
information product contains certain toxic or
hazardous substances. The center number is the
Environmentally Friendly Use Period (EFUP) date,
and indicates the number of calendar years the
product can be in operation. Upon the expiration
of the EFUP, the product must be immediately
recycled. The circling arrows indicate the product
is recyclable. The date code on the label or
product indicates the date of manufacture.
California Proposition
65 Symbol
This label indicates that this product can expose
you to chemicals including phthalates, which are
known to the State of California to cause cancer
and birth defects or other reproductive harm. For
more information go to
www.P65Warnings.ca.gov.
Symbol Warning Condition Action

C37808AC xv
Safety Notices
Symbol Explanations
Laser Class I This label indicates that this product is a Class I
Laser product. Take precautions to prevent
exposure.
Consider all materials
(specimens, controls,
monoclonal
antibodies, and so
forth) as being
potentially infectious.
Wear standard laboratory attire and follow safe
laboratory procedures when handling any
material in the laboratory.
UKCA Mark A "UKCA" mark indicates that a product has been
assessed before being placed in UK market, and
has been found to meet UK safety, health, and/or
environmental protection requirements.
Symbol Warning Condition Action
C
LA
SS
1 LA
S
ER PR
O
DU
C
CLASS 1 LASER PRODUCT
PRODUIT LASER CLASSE 1

xvii
Contents
Revision History,iii
Safety Notices,vii
Alerts for Warning, and Caution,vii
Safety Precautions,viii
Instrument Safety Precautions,ix
General Safety,ix
Biohazard Safety,x
Electrical Safety,xi
Safety Interlocks,xi
Stream Charge,xi
Drop Drive Voltage,xi
Deflection Plates,xii
Deflection Plate Arcing,xii
Laser Power Supplies,xii
Electromagnetic Compatibility,xii
Symbol Explanations,xiii
Introduction, xxxi
Overview,xxxi
How to Use Your Manual,xxxi
About this Manual,xxxi
Conventions Used,xxxii
Graphics,xxxiii
CHAPTER 1: System Overview,1-1
Overview,1-1
Product Description,1-1
Operation Principles,1-1
Main Components,1-3
Fluidics System,1-5
Fluidics Cart,1-6
Sheath Tank,1-9
Shutdown Fluid Container,1-10
Waste Container,1-11
Fluidics Module,1-12
Sample Station,1-13

xviii
Contents
Optical Components,1-16
Laser Beam Shaping,1-17
Cell Illumination,1-17
Forward Scatter,1-17
Side Scatter and Fluorescent Light,1-17
Forward Scatter Collection,1-18
Side Scatter and Fluorescent Light Collection,1-18
Side Scatter,1-18
Fluorescent Light,1-18
Wavelength Division Multiplexer (WDM),1-19
Optical Fiber,1-22
Cell Sorting,1-23
Sort Chamber,1-23
Stream Camera and Droplet Monitor,1-25
Deflection Plates,1-26
Nozzle Module,1-27
Waste Catcher,1-28
CyClone Movement System,1-29
Sample Temperature Control,1-31
Aerosol Evacuation System,1-34
Instrument Electronics,1-36
System Connections,1-36
Instrument Specifications,1-40
Dimensions,1-40
Installation Category,1-41
Maximum Altitude,1-41
Pollution Degree,1-41
Acoustic Noise Level,1-41
Electrical Ratings,1-41
Sorter,1-42
Performance Characteristics,1-43
Material Safety Data Sheets (SDS/MSDS),1-44
CHAPTER 2: Using the CytExpert SRT Software,2-1
Overview,2-1
Launching the Software,2-1
Main Software Screen,2-1
Start Page,2-2
Acquisition Screen,2-3
Acquisition Screen Navigation,2-4
Collection,2-5
Acquisition/Sorting Control,2-7
Tube Management,2-8
Plot Area,2-10
Status Bar,2-11

xix
Contents
Analysis Screen,2-11
Compensation Experiment Screen,2-12
QC Experiment Screen,2-14
QC Report Screen,2-14
QC Experiment Screen,2-15
QC Screen Navigation,2-15
Software Menu,2-16
Acquisition and Analysis Screen Menu,2-17
User Management,2-21
Creating, Deleting, and Modifying Users in User Manager,2-23
Unlocking a User Account,2-25
Resetting a User Password,2-26
Changing a User Password,2-27
Forgot Username or Password,2-28
Role Management,2-30
Creating, Deleting, and Modifying User Roles in Role Manager,2-32
Account Policies,2-35
Operation Log,2-37
Viewing and Exporting Experiment Operation Logs,2-38
Viewing and Exporting System Operation Logs,2-40
Viewing and Exporting User Management Operation Logs,2-42
Graphic and Gating Styles,2-43
Plots,2-43
Gates,2-44
Collection Device Library,2-45
Sort Mode Library,2-47
Sort Mode,2-47
Sort Precision Level,2-48
Drop Envelope,2-49
Sort Guard Band,2-50
Sort Stream Precedence,2-51
Adding a Sort Mode,2-52
Deleting a Sort Mode,2-55
Software Settings,2-57
Language Settings,2-63
CHAPTER 3: Daily Startup,3-1
Overview,3-1
Pre-Startup Inspection,3-1
System Connections Inspection,3-1
Checking Fluid Levels,3-2
Turning On the Instrument,3-3
Logging Into the Software,3-4

xx
Contents
System Startup Program,3-8
Selecting Experiments from the Start Page,3-18
CHAPTER 4: Instrument Quality Control and Standardization,4-1
Overview,4-1
Quality Control,4-2
Preparing the QC Sample,4-3
Required Materials,4-3
CytoFLEX Daily QC Fluorospheres Preparation Process,4-3
CytoFLEX Ready to Use Daily QC Fluorospheres Preparation
Process,4-4
Importing Lot-Specific Target Values,4-4
Collecting QC Data,4-10
Confirming Results,4-13
QC Result Manager,4-20
Standardization,4-21
Preparing the Standardization Sample,4-21
Required Materials,4-22
Preparation Process,4-22
Generating Target Median Values,4-22
Adding a New Standardization Item,4-29
Performing the Standardization,4-33
Applying the Standardized Acquisition Settings,4-36
Standardization Target Library,4-39
CHAPTER 5: Sorting,5-1
Overview,5-1
Sort Calibration (Auto Drop Delay),5-2
Default Amplitude Setting (Optional),5-8
Setting Drop Delay Manually (Optional),5-9
Creating an Experiment,5-20
Creating an Experiment,5-20
Changing the Tube Name,5-21
Setting the Channel and Label,5-22
Creating Plots and Gates,5-25
Creating and Adjusting Auto Gates,5-35
Turning Auto Recalculate On/Off,5-37
Adjusting Autogate Movement and Extent,5-38
Configuring Acquisition Settings,5-40
Laser Settings,5-40
Adjusting the Gain,5-42
Adjusting the Threshold,5-44
Setting Collection Conditions,5-46
Setting Sample Flow Rate,5-47
Setting Mixing Speed,5-48

xxi
Contents
Setting Plot Display Conditions,5-49
Setting Customized Parameters,5-50
Setting Custom Statistics,5-51
Load Sample and Record Data,5-55
Before Running Samples,5-55
Setting Up Violet Side Scatter (VSSC) Channel,5-56
Verifying, Selecting, Editing, and Creating Detector
Configuration,5-60
Sampling and Collecting Data,5-63
Sorting,5-67
Setting Up Tube Sorting,5-67
Setting Up Plate/Slide Sorting,5-73
Stop Criteria for Sorting,5-80
Calibrating the Sort Collection Device,5-81
Manual Side Stream Calibration (Optional),5-89
Adjusting Tube Position (Optional),5-93
Starting and Monitoring a Sort,5-94
During a Sort,5-94
Viewing Sorter Status,5-95
Side Stream Monitor,5-96
Auto Maintain,5-97
Viewing Sorting Statistics,5-102
Pause Sorting and Resume Sorting,5-104
Index Sorting,5-105
Sort Report,5-108
Creating a Sort Report,5-108
Designing a Report,5-109
Report Output,5-110
Additional Information for Aseptic Sorting,5-111
Exporting Data,5-111
Exporting FCS Files,5-117
Exporting Plots or the Statistics Table of Multiple Tubes as Picture
Files,5-120
Importing and Exporting Instrument Settings,5-121
Importing and Exporting Compensation Settings,5-123
Printing Graphics,5-124
Saving the Experiment,5-126
Concluding the Experiment,5-126
CHAPTER 6: Compensation,6-1
Overview,6-1
Creating a Compensation Experiment,6-2
Preparing the Compensation Sample,6-5
Using Control Samples to Generate the Compensation Matrix,6-6
Calculating Compensation Values,6-10
Creating the Compensation Matrix from Previously Acquired Data,6-11