Beckman Coulter Avanti J-26S XP User manual

PN B10087AB
June 2012
Instructions For Use
Avanti J-26S XP
High-Performance Centrifuge
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821

Avanti J-26S XP
High-Performance Centrifuge
PN B10087AB (June 2012)
Copyright © 2012 Beckman Coulter, Inc.
All rights reserved
No part of this document may be reproduced or
transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Beckman Coulter, the stylized logo, and Avanti are
trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Made in the USA
Printed in the USA

PN B10087AB iii
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Danger, Warning, Caution, Important, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This instrument is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the instrument. Also, if the instrument needs to be moved, a Beckman Coulter Field
Service representative must reinstall and relevel the instrument in its new location.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off and the
instrument is disconnected from the main power source by removing the Mains (power) plug from
the outlet receptacle, and refer such servicing to qualified personnel.

PN B10087AB
iv
Safety Notice
Electrical Safety
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system is
designed to reduce the possibility of injury or damage that could result from instrument movement
in the event of a major rotor mishap.
Do not replace any centrifuge components with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this instrument uses a three or five-wire electrical cord and
plug to connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
This instrument is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (for example, chloroform or ethyl alcohol) in
this instrument nor handle or store them near the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not lift or move the centrifuge while a rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Do not lean on the centrifuge or place items on it while it is operating.

PN B10087AB v
Safety Notice
Chemical and Biological Safety
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument unless all necessary safety
precautions are taken.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting service
by Beckman Coulter Field Service.

PN B10087AB
vi
Safety Notice
Chemical and Biological Safety

vii
Contents
Safety Notice,iii
Alerts for Danger, Warning, Caution, Important, and Note,iii
Safety During Installation and/or Maintenance, iii
Electrical Safety,iv
Safety Against Risk of Fire,iv
Mechanical Safety,iv
Chemical and Biological Safety,v
Introduction,xiii
Certification,xiii
Scope of Manual,xiii
Conventions,xiv
CFC-Free Centrifugation,xiv
Recycling Label,xiv
CHAPTER 1: Description,1-1
Introduction,1-1
Centrifuge Function and Safety Features,1-1
Centrifuge Function,1-1
Safety Features,1-2
Centrifuge Chassis,1-2
Housing and Door,1-2
Rotor Chamber,1-2
Drive,1-3
Friction Reduction System (FRS),1-3
Temperature Sensing and Control,1-3
Overtemp System,1-3
Name Rating Plate,1-3

viii
Controls and Indicators,1-4
Power Switch,1-4
Control Panel,1-4
Specifications,1-7
Control Features,1-7
Operational Features,1-8
Physical Data,1-9
Available Rotors,1-10
CHAPTER 2: Operation,2-1
Introduction,2-1
Summary of Avanti J-26S XP Run Procedures,2-2
Section and Loading,2-3
Entering Run Parameters,2-4
Selecting a Rotor,2-4
Setting Run Speed,2-4
Setting Run Time,2-5
Setting Run Temperature,2-7
Setting Acceleration and Deceleration Rates,2-7
Setting Up an Elutriation Run,2-9
Starting a Run,2-11
Changing Parameters During a Run,2-11
Changing Time Settings,2-12
Stopping a Run,2-12
Unloading the Centrifuge,2-13
CHAPTER 3: Troubleshooting,3-1
Introduction,3-1
User Messages,3-1
Accessing the Rotor in Case of Power Failure,3-5
JCF-Z Rotor Identification,3-10
CHAPTER 4: Care and Maintenance,4-1
Introduction,4-1
Maintenance,4-1
Cleaning,4-2
Decontamination,4-3
Sterilization and Disinfection,4-3
Replacing the Air Filter,4-4
Circuit Breaker and Fuses,4-4

ix
Storage and Transport,4-5
Storage,4-5
Returning a Centrifuge,4-5
Supply List,4-6
Replacement Parts and Supplies,4-6
APPENDIX A: Preinstallation Requirements,A-1
Preinstallation Requirements,A-1
Electrical Requirements,A-1
Space and Location Requirements,A-4
Securing the Centrifuge to the Floor,A-6
Using J2 Series Rotors in the Avanti J-26S XP,A-6
Checking for Rotor Drive Pins,A-6
Using the JA-18 Rotor,A-7
Using the JCF-Z Continuous Flow/Zonal Rotor,A-8
APPENDIX B: Temperature Calibration Procedure,B-1
Introduction,B-1
Beckman Coulter, Inc.
Avanti J Series Centrifuge Warranty
Related Documents

x

xi
Illustrations
Illustrations
1.1 The Power Switch,1-4
1.2 The Control Panel,1-4
2.1 Acceleration and Deceleration Settings Graph,2-8
2.2 Elutriation Controls,2-9
3.1 Emergency Door Release Latch Access,3-5
3.2 Manual Door Release,3-6
3.3 Venting the Chamber Vacuum,3-8
3.4 Front Panel Retaining Strip,3-9
A.1 Single-Phase Electrical Requirements,A-3
A.2 Three-Phase “Y” Electrical Requirements,A-3
A.3 Correct and Incorrect Three-Phase Power Configurations,A-4
A.4 Rear View and Dimensions,A-5
A.5 Checking the Rotor for Drive Pins,A-7

xii
Tables
Tables
2.1 Acceleration Settings,2-7
2.2 Deceleration Settings,2-8
3.1 Diagnostic Message Chart,3-2
A.1 Nominal Supply Voltage Ratings for the Avanti J-26S XP,A-2
A.2 Required Wire Connections,A-3

PN B10087AB xiii
Introduction
Certification
Beckman Coulter Avanti J-26S XP centrifuges are manufactured in a facility that maintains
certifications to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be
compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.
Scope of Manual
This manual is designed to familiarize centrifuge users and site engineers with the Avanti J-26S XP
centrifuge, its functions, specifications, operation, and routine care and maintenance.
Beckman Coulter recommends that you read this entire manual, especially the Safety Notice and
all safety-related information, before operating the instrument or performing instrument
maintenance.
• CHAPTER 1, Description contains an overall description of the centrifuge, including a
description of system controls and indicators, and system specifications.
• CHAPTER 2, Operation summarizes procedures for operating the centrifuge.
• CHAPTER 3, Troubleshooting lists system diagnostic messages, together with probable causes
and user-performed corrective actions.
• CHAPTER 4, Care and Maintenance contains procedures for routine maintenance, as well as a
brief list of supplies and replacement parts.
• APPENDIX A, Preinstallation Requirements contains instructions for preparing your site for
centrifuge installation.
• APPENDIX B, Temperature Calibration Procedure contains a procedure to use when temperature
control within ±1°C is required.
NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual is the sole responsibility of the user.

PN B10087AB
xiv
Introduction
Conventions
Conventions
Certain symbols are used in the product labeling to call out safety related and other important
information. These international symbols may also be displayed on the centrifuge and are
reproduced on the inside back cover of the manual.
Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.
• Control knob names (for example, ROTOR and SPEED) appear in bold type.
• Key names (for example, START and STOP) appear in bold type.
• Display field names (for example, TEMP°C or SPEED) appear in bold type.
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Avanti J-26S XP centrifuge.
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
1. The device was put on the European market after August 13, 2005 and
2. The device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
A28219-AA

PN B10087AB 1-1
CHAPTER 1
Description
Introduction
This section describes the Avanti J-26S XP centrifuge components and their functions. It also describes
system safety features and centrifuge controls and displays. Refer to the applicable rotor manual for rotor
descriptions.
Centrifuge Function and Safety Features
Centrifuge Function
The Avanti J-26S XP is a refrigerated centrifuge that generates centrifugal forces required for a wide
variety of applications. Together with the Beckman Coulter rotors designed for use in this
centrifuge, applications include:
• Routine processing such as sample preparations, pelleting, extractions, purifications,
concentrations, phase separations, and spin column and spin filter centrifugations.
• Rapid sedimentation of protein precipitates, large particles, and cell debris.
• Preparation of subcellular organelles such as mitochondria, nuclei, chloroplasts, and crude
microsomes.
• Separation of blood cells and cellular components.
• Pelleting of prokaryotic and eukaryotic cells.
• Gradient separation, for example, Ficoll-Hypaque and Percoll.
• Nucleic acid precipitation.
• Virus isolation.
• Bacteriophase isolation.
The Avanti J-26S XP centrifuge is microprocessor-controlled, providing interactive operation.*The
centrifuge features a brushless switched-reluctance drive motor,†an automatic rotor identification
system, FRS (friction reduction system), vacuum control circuitry, and a temperature control
system with automatic temperature compensation unique for each compatible rotor.
* Avanti J-26 XP software and firmware ©2005–2012 Beckman Coulter, Brea, CA, 92821.
† Manufactured under license from Switched Reluctance Drives Limited, Harrogate, U.K.

PN B10087AB
1-2
Description
Centrifuge Chassis
The user interface has a digital display, which can be toggled to show either actual centrifuge values
or user-entered setup values, knobs for parameter entry, and touch keys. Diagnostic messages on
the control panel will light to alert the operator to conditions that may need attention.
Safety Features
Avanti J-26S XP centrifuges have been designed and tested to operate safely indoors at altitudes up
to 2000 m (6562 ft).
Safety features include the following.
• An electromechanical door lock system prevents operator contact with spinning rotors and
prevents run initiation unless the door is shut and locked. The door locks when START is
pressed, or when the POWER switch is turned off.
• A steel casing surrounds the rotor chamber to provide operator protection in the unlikely event
of a rotor mishap.
• An automatic rotor identification system detects which rotor is installed and prevents the rotor
from running above its maximum rated speed.
• An imbalance detector monitors the system during operation, causing automatic shutdown if
rotor loads are severely out of balance.
Centrifuge Chassis
Housing and Door
The instrument control housing, door assembly, and cover panels are made from a highly-durable
unfinished plastic. The control panel is covered by a protective overlay made of coated
polycarbonate.
The door is opened by stepping on a foot pedal, which is located at the bottom right front of the
instrument. The door is hinged at the back left to open at a 60-degree angle to the centrifuge side
panels, providing clearance for loading and unloading of the centrifuge.
In the event of a power failure, the door can be manually unlocked for sample recovery (see
CHAPTER 3, Troubleshooting).
Rotor Chamber
The rotor chamber is made of stainless steel to resist corrosion. A rubber gasket around the
chamber opening ensures sealing. (Instrument gaskets have not been qualified as bioseals for
aerosol containment.)

PN B10087AB 1-3
Description
Name Rating Plate 1
Drive
The drive spindle is directly driven by a brushless, high-torque, switched-reluctance motor. The
instrument’s resilient suspension minimizes disturbance of the sample during acceleration and
deceleration, and reduces damage to the drive spindle if an imbalance occurs during centrifugation.
Friction Reduction System (FRS)
The friction reduction system (FRS) uses a mechanical rotary vane vacuum pump to reduce
chamber pressure to approximately one-quarter atmosphere (190 mm Hg). The pump turns on after
the run is started, before rotor friction reaches a high level. When the required vacuum level is
reached, the pump turns off. Vacuum in the chamber is vented during rotor deceleration.
Temperature Sensing and Control
The temperature control system is cooled by circulation of a non-CFC-based refrigerant. The
temperature control system is activated when the centrifuge power is on and when the door is
closed.
A thermistor in the rotor chamber continuously monitors the chamber temperature. The system
calculates the chamber temperature required to maintain the set rotor temperature, 2°C.*
Although the chamber temperature fluctuates during operation, the rotor’s large mass keeps the
sample temperature substantially constant. At the end of a run, the system continues controlling
the temperature to prevent freezing or overheating (more than 4°C over set temperature) of
the sample.
Overtemp System
An overtemp (over temperature) system provides sample protection and safety for the user.
• During a run, if the system temperature rises 4°C above the set temperature, the TEMP
diagnostic will light. The run will continue, unless stopped by the user. If the temperature rises
8°C above the set temperature, the system will shut down (using maximum brake).
• The system will always shut down (using maximum brake) if the system temperature reaches
50°C. The centrifuge cannot be restarted until the chamber has cooled.
Name Rating Plate
The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with
the voltage listed on this name rating plate before connecting the centrifuge. Always mention the
serial number and the model number shown when corresponding with Beckman Coulter regarding
your centrifuge.
* Refer to the applicable rotor manual for specific temperature information.

PN B10087AB
1-4
Description
Controls and Indicators
Controls and Indicators
Power Switch
The power switch is located below the control panel (see Figure 1.1). This two-position rocker
switch (I, on; O, off) controls electrical power to the centrifuge.
Figure 1.1 The Power Switch
Control Panel
The control panel (Figure 1.2) is mounted at an angle on the centrifuge top rear for easy visibility
and access. It contains a digital display for actual and setup values, knobs and touch keys for
parameter entry, and additional touch keys for system control.
Figure 1.2 The Control Panel
1. Digital Display
1
ROTOR ID SPEED TEMP
RPM/RCF TIME HR:MIN
START
RCG(xg)
rad 2/sec
STOP
°c SET
ACTUAL
AUTO
RPM
HOLD
EXP
JCF
JSP
JS
JA
ROTOR SPEED
TIME/
HOLD
TIME
SET/
ACTUAL
TEMP ˚C
JLA
JV
JE
ACCEL
MAX
SLOW
MAX
SLOW
OFF
FRS
ROTOR
IMBAL
SPEED
DOOR
SYS
POWER
DRIVE
REFR
TEMP
RPM /
RCF
DECEL CLEAR

PN B10087AB 1-5
Description
Controls and Indicators 1
Digital Display
The display shows current status of the instrument whenever the power is turned on.
• The display shows both actual centrifuge values and user-set parameters. The SET/ACTUAL key
is used to toggle between the two views.
• During a run, actual values are displayed. If you turn a knob (other than the TIME knob) during
operation, the display will change to show the set values. If no parameters are entered or
changed for 5 seconds, the display will revert to showing actual values.
• During a timed run, the TIME display shows the remaining run time in hours and minutes. The
TIME display begins counting down when START is pressed and continues counting down to 0,
when deceleration begins. When the rotor decelerates to 0 RPM, End flashes in the TIME display.
• When HOLD is selected (no run time is specified), the TIME display shows the time elapsed since
START was pressed. After 3 hours (180 minutes) have elapsed, the timer will reset to 0 and
continue counting elapsed time until STOP is pressed.
Knobs and Touch Keys
The rotor name, speed, time, and temperature settings are entered by turning the appropriate knob
until the required value is displayed.
Touch keys allow the user to start and stop the centrifuge, and to select speed mode (RPM or RCF),
time mode (specific run length or hold for continuous operation), and acceleration and deceleration
rates, and to toggle the display between set and actual values.
ROTOR Knob Used to select the rotor in use. As the knob is turned, the name of each
Beckman Coulter rotor that can be run in the centrifuge appears in succession in
the ROTOR ID display, and the LED by each rotor type abbreviation
(JCF: continuous flow rotors; JSP: special type rotors; JS: swinging bucket rotors;
JA: fixed angle rotors; JLA: lightweight fixed angle rotors; JV: vertical tube rotors;
JE: elutriator rotors) lights when appropriate.
SPEED Selection The RPM/RCF key is pressed to select the speed mode (RPM or RCF). After the
mode is selected, the SPEED knob functions as follows.
• In RPM mode, each SPEED knob increment is 100 RPM at speeds up to
10,000 RPM. Above 10,000 RPM, each knob increment is 500 RPM.
• In RCF mode, at settings less than or equal to 3000 g, each SPEED knob
increment is equivalent to incrementing/decrementing the speed by 100 g.
At settings greater than 3000 g, each SPEED knob increment is equivalent to
incrementing/decrementing the speed by 250 g.
• Speed increments for elutriator rotors are either 10 RPM or 10 g.
ROTOR
JSP
JS JA JLA
JV
JE
JCF

PN B10087AB
1-6
Description
Controls and Indicators
TIME Selection Two time modes are available. The HR:MIN mode is used for runs of specified
lengths. The HOLD mode is used for continuous runs of unspecified lengths. The
HOLD key is used to toggle between the two modes.
In HR:MIN mode:
• The TIME knob is used to set run time, which can be set up to 3 hours and
0 minutes (180 minutes).
• Each TIME knob increment is 1 minute. A full turn of the knob equals
60 minutes.
• The time setting can be changed during a run.
• During a run, the time remaining in the run is displayed. When 0 is reached, the
run ends and the rotor decelerates to a stop.
In HOLD mode:
• When HOLD mode is selected, the time display shows the word HOLD.
• During a run, the time elapsed is displayed. When the display reaches 3 hours
and 0 minutes, the system automatically resets the display to 0 and begins
counting the elapsed time again. The run will continue until STOP is pressed.
• If you switch from HOLD mode to HR:MIN mode during a run, the system
remembers the previous time setting. Turning the TIME knob increments or
decrements the set time from that point.
TEMPERATURE
Knob
Used to select the rotor temperature, from –10 to +40°C.
• The minimum and maximum allowable set temperatures depend on the set
speed and the rotor in use. If a temperature is entered that cannot be achieved
by the installed rotor at the set speed, the TEMP°C field will flash.
• The maximum achievable rotor temperature depends on the frictional heat
generated inside the chamber during operation. At low run speeds or low
ambient temperature, the centrifuge may not be able to achieve some higher
temperatures.
ACCELERATION
Key
Used to select either maximum or slow acceleration. An indicator light above the
ACCEL key displays the selection. Acceleration rates are described in Ta b l e 2 . 1 ).
DECELERATION
Key
Used to select maximum or slow deceleration, or off (no brake). An indicator light
above the DECEL key displays the selection. Deceleration rates are described in
Ta b l e 2 . 2 .
ACCEL
MAX
SLOW
DECEL
MAX
SLOW
OFF