Beckman Coulter CytoFLEX LX User manual

Put the instrument in standby mode by selecting Standby under the Cytometer tab.
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CytoFLEX LX
Replace Sheath Fluid Filter and Sample Tubing
Workflow Overview
Replacement of Sheath Fluid Filter and Sample Tubing to be carried out by FCCF staff every 6
months.
Ver. #1
2022-04-04
Instrument
in standby
Replace the
Sheath Filter
Replace the
Sample
Tubing
1. Instrument in Standby
Remove the right-side cover.
•Open the top cover.
•Unfasten the two thumbscrews for the right-side cover.
•Lift the right-side cover up and out of the slots in the frame.
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Image from CytoFLEX LX User Manual

2. Replace the Sheath Filter
Press the spring piece on the quick connector (1) on the upper side of the filter and disconnect
it (2). Repeat this step for the quick connector located behind the one already removed.
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Remove the sheath fluid filter from the bracket, connect the new filter using the connector
springs, and place the filter back in the bracket.
2
Remove the vent cap and the rubber insert and put it to the side.
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3. Replace the Sample Tubing
Open the software, turn on the cytometer and run a Prime, in the Cytometer tab.
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During the Prime cycle, pay attention to the fluid level in the filter. When the liquid reaches the
upper part of the filter, put the rubber insert back and close the vent cap to avoid air leakage.
Note: If the vent cap isn’t sealed tightly, leakage of the sheath fluid can occur and result in an unstable flow
rate.
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In addition to the removal of the side cover, the front cover also needs to be removed.
•Open the top cover;
•Remove the two screws of the front cover (1);
•Pull the front cover forward (2).
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Remove the sample pump cover thumbscrew (1) and then the sample cover (2).
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Take out the sample peristaltic tubing and remove the sample PEEK tubing.
3
Lift the sample probe out of the wash station and remove the sample tubing.
4
Dispose the old sample tubing in the Biohazard trash bin.
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Connect the new sample tubing to the sample probe.
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Connect the sample PEEK tubing to the sample peristaltic pump tubing and slide the sample
probe back to the wash station.
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Insert the sample tube back into the groove.
Note: Do not use any sharp tool to help you put the peristaltic tubing back.
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Reinstall the sample cover, the front cover, and the right-side cover.
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