Beckman Coulter Optima TLX User manual

TLX-IM-9
Instruction Manual
Optima™ TLX
Preparative Ultracentrifuge
TM

Symbol
Symbol
Symbole
Símbolo
Title / Titel / Titre / Titulo / Titolo /
Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!
/
Simbolo

SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, mainte-
nance, or repair of this centrifuge. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
centrifuge.
Safety During Installation and/or Maintenance
This ultracentrifuge is designed to be installed by a Beckman Coulter Field Service repre-
sentative. Installation by anyone other than authorized Beckman Coulter personnel invali-
dates any warranty covering the instrument.
This ultracentrifuge weighs 81 kg (178 lb). Do not attempt to lift or move it without
assistance.
Any servicing of this equipment that requires removal of any covers can expose parts
which involve the risk of electric shock or personal injury. Make sure that the power
switch is off and the centrifuge is disconnected from the main power source, and refer
such servicing to qualified personnel.
Do not replace any ultracentrifuge components with parts not specified for use on this
instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect the ultracentrifuge to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-
grounded. Check that the line voltage agrees with the voltage listed on the name-rating
plate affixed to the centrifuge.
•Never use a three-to-two wire plug adapter.
•Never use a two-wire extension cord or a two-wire non-grounding type of multiple-
outlet receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
Fuses protect certain electrical circuits within this instrument against overcurrent condi-
tions. For continued protection against the risk of fire, replace only with the same type and
rating specified.
!

This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this centrifuge nor handle or store them within the required 30-cm (1-ft) area
surrounding the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the rotors and accessories designed for use in this centrifuge.
• Do not exceed the maximum rated speed of the rotor in use.
• Do not lift or move the centrifuge while the rotor is spinning.
• NEVER attempt to slow or stop the rotor by hand.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the ultracen-
trifuge for at least 1 hour. Then follow the instructions for sample recovery in
Section 4,
TROUBLESHOOTING
.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless
all necessary safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent—
Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic
fungi—further emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
centrifuge without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual ) are handled; materials of a higher group require more
than one level of protection.
•Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the centrifuge and accessories before
requesting service by Beckman Coulter.

TLX-IM-9
February 2007
© 2007 Beckman Coulter, Inc.
Printed in the U.S.A.

Contents
iii
Page
INTRODUCTION
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Notes, Cautions, and Warnings. . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Typographic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Canadian Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
SECTION 1: DESCRIPTION
Description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Vacuum System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Temperature Sensing and Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Drive. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Power Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Control Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Barrier Ring. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Imbalance Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Overspeed and Rotor Identification System . . . . . . . . . . . . . . . . . 1-9
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11
SECTION 2: PREINSTALLATION REQUIREMENTS
Space Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

iv
Contents
Page
SECTION 3: OPERATION
Summary of Optima TLX Run Procedures . . . . . . . . . . . . . . . . . . . . 3-2
Programmed Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Manual Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Installing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Pre-Run Cooling or Warming . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Manual Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Entering Run Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Entering Run Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Entering Run Temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Entering Acceleration and Deceleration Rates . . . . . . . . . . . . . . 3-8
Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Creating a New Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Starting a Programmed Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Recalling, Changing, or Deleting a Program . . . . . . . . . . . . . . . 3-13
SECTION 4: TROUBLESHOOTING
User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Retrieving Your Sample in Case of Power Failure . . . . . . . . . . . . . . 4-4
Circuit Breaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
SECTION 5: MAINTENANCE
Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Vacuum System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Drive Hub. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Air-Intake and Exhaust Louvers . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Sterilization and Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Storage and Transportation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Warranty

Illustrations and Tables
v
Page
Figure 1-1. The Optima TLX Ultracentrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Figure 1-2. Temperature Control Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Figure 1-3. Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Figure 2-1. Dimensions of the Optima TLX Ultracentrifuge . . . . . . . . . . . . . . . . 2-2
Figure 4-1. Location of User Messages on the Control Panel . . . . . . . . . . . . . . . 4-2
Figure 4-2. Removing the Top Cover of the Instrument. . . . . . . . . . . . . . . . . . . . 4-5
Figure 4-3. Door Lock System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Table 1-1. Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11
Table 3-1. Acceleration and Deceleration Rates . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
Table 4-1. User Message Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

vii
CERTIFICATION
To ensure full system quality, Beckman Coulter Optima™ TLX
ultracentrifuges are manufactured in a registered ISO 9001 facility.
They have been designed and tested to be compliant (when used with
Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and
certificates of compliance are available at www.beckmancoulter.com.
SCOPE OF MANUAL
This manual is designed to familiarize you with the Beckman Coulter
Optima™ TLX ultracentrifuge, its functions, specifications, opera-
tion, and routine operator care and maintenance. We recommend that
you read this entire manual, especially the
SAFETY NOTICE
and all
safety-related information, before operating the ultracentrifuge or
performing instrument maintenance.
• Section 1 contains system specifications and a brief physical and
functional description of the ultracentrifuge, including the
operating controls and indicators.
• Section 2 provides information about space and power require-
ments for installing and connecting the ultracentrifuge.
• Section 3 contains ultracentrifuge operating procedures.
• Section 4 lists possible malfunctions, together with probable
causes and suggested corrective actions.
• Section 5 contains procedures for routine operator care and main-
tenance, as well as a brief list of supplies and replacement parts.
Introduction

viii
Introduction
➠NOTE
If the ultracentrifuge is used in a manner other
than specified in this manual, the safety and
performance of this equipment could be
impaired. Further, the use of any equipment
other than that recommended by Beckman
Coulter has not been evaluated for safety. Use of
any equipment not specifically recommended in
this manual and/or the applicable rotor manual is
the sole responsibility of the user.
CONVENTIONS
Certain symbols are used in this manual to call out safety-related and
other important information. These international symbols may also be
displayed on the centrifuge and are reproduced and described below
and on the inside of the front cover.
NOTES, CAUTIONS, AND WARNINGS
➠NOTE
Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment.
!CAUTION
Used to indicate a potentially hazardous situa-
tion which, if not avoided, may result in minor
or moderate injury and/or mechanical damage.
It is also used to alert against unsafe practices.

ix
Introduction
!WARNING
Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.
WARNING
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
either symbol to service personnel.
TYPOGRAPHIC CONVENTIONS
Certain typographic conventions are used throughout this manual to
distinguish names of user interface components, such as keys and
displays.
•Keynames (for example, [START] or [PROG]) appear in capital letters
within brackets. (The [ce] key is not in capitals.)
•Display names (for example, SPEED rpm or TIME hr:min)
appear in bold type.
CFC-FREE CENTRIFUGATION
To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of the Optima TLX ultracentrifuge.
CFC

x
Introduction
RADIO INTERFERENCE
This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ-
ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica-
tions. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.
CANADIAN REGULATIONS
This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.
RECYCLING LABEL
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:
1) the device was put on the European market after August 23, 2005
and
2) the device is not to be disposed via the municipal waste collection
system of any member state of the European Union.
It is very important that customers understand and follow all laws
regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.
A28219-AA
Note: On the instrument, the
triangle background is yellow
rather than gray.

1
1-1
DESCRIPTION
The Optima TLX (see Figure 1-1) microprocessor-controlled tabletop
ultracentrifuge generates high centrifugal forces for a variety of high-
speed applications. The instrument design features a variable-fre-
quency induction drive, thermoelectric temperature control system,
self-purging vacuum system, rotor overspeed identification system,
program memory that can contain up to ten five-step programs, and
a choice of acceleration and deceleration rates.
Figure 1-1. The Optima TLX Ultracentrifuge
TM
Description

1-2
Description
Manual and programmed operation are available.
• In manual operation, you enter the individual run parameters
before beginning each run.
• In programmed operation, you can duplicate runs quickly and
accurately by selecting one of ten programs previously entered
into the program memory. Each program can contain up to
five steps.
User messages and/or audible signals are provided to alert you to con-
ditions that may need attention.
ROTOR CHAMBER
The aluminum chamber is coated with a chemical-resistant epoxy
finish to resist corrosion. The rotor drive hub and speed sensors are
visible in the bottom of the chamber.
VACUUM SYSTEM
A diffusion pump, in series with a mechanical vacuum pump, reduces
chamber pressure to below 10 microns (1.34 Pa). The vacuum pump
starts automatically when the power is turned on and the door is
closed. The diffusion pump is automatically activated when chamber
pressure reaches about 500 microns. When the instrument is on and
the door closed, a self-purging system continuously removes moisture
from the vacuum system.
The VAC message appears on the control panel any time the power is
on and the vacuum is above 500 microns. It also appears if a chamber
pressure of 10 microns or less is not reached after 45 minutes. At
speed over 5000 rpm, a rise in the vacuum level to over 500 microns
will cause the message to flash and the instrument to decelerate to
5000 rpm until the vacuum level goes below 500 microns. The instru-
ment will then accelerate to the set speed. If the vacuum remains
above 500 microns for more than 20 minutes, the instrument will
issue a VAC diagnostic and brake to a stop.
The vacuum is released when the [DOOR] key is pressed while
the rotor is at rest. If you decide not to open the door after pressing
the key, the vacuum system will automatically start again after 12 to
14 seconds.

1-3
Description
TEMPERATURE SENSING AND CONTROL
The solid-state thermoelectric temperature control system uses only
forced air—no coolant is required. With the power on, the tempera-
ture control system is activated when the door is closed and the
vacuum system comes on. Run temperature can be set between 0 and
40°C. If no set temperature is entered, the instrument automatically
selects the last entered temperature. (For the first run of a new instru-
ment, 25°C is the default operating temperature.)
A sensor in the rotor chamber continuously monitors chamber
temperature. The microprocessor calculates the required chamber
temperature to maintain the selected rotor temperature. Peak-to-peak
fluctuations of rotor temperature (after reaching thermoequilibrium)
will be within 0.1°C (see Figure 1-2).
Figure 1-2. Temperature Control Diagram
DRIVE
The air-cooled direct-drive induction motor is frequency controlled,
with no gears or brushes. In addition, the drive does not require an oil
vacuum seal or external oil reservoir. Externally cooled by forced air
and internally cooled by oil, the drive delivers ultra-smooth, quiet
performance.
Time
Set Temperature
+2°
-2°
Actual rotor temperature
0.1°(peak-to-peak
fluctuation)
°C

1-4
Description
CONTROLS AND INDICATORS
POWER SWITCH
The power switch controls electrical power to the ultracentrifuge. It is
also a circuit breaker that will trip to cut off power in the event of a
power overload. The power switch must be turned on before the
chamber door can be opened.
CONTROL PANEL
The control panel (see Figure 1-3) is used to enter run parameters via
touch keys and a keypad, and to display run parameters and program
information. A beep sounds when a key has been pressed and the
input accepted.
Figure 1-3. Control Panel
!POWER
I
O
SPEED rpm TIME hr:min TEMPERATURE˚C
DOOR
PROGRAM
PROG
SAVE PROG
DELDEL START STOP
STEP ACCEL DECEL
STEP ACCEL DECEL
SPEED
TIME
TEMP
789
456
123
0
ENTER DISPLAY
±ce
: .
Digital Displays Digital Displays
Run-Parameter Keys
Keypad
Power Switch

1-5
Description
Digital Displays
The displays serve a dual purpose.
• During a run, they show the actual (real-time) operating
conditions.
• When the run parameters are being entered, they show the set
values selected. In manual operation, these set values are retained
in memory until new values are entered. They can be recalled by
pressing [ENTER/DISPLAY]. After 5 seconds, the displays return to
actual conditions.
SPEED rpm Indicates rotor speed in revolutions per minute (rpm). Speeds
between 0 and 5000 rpm are shown in increments of 10; speeds above
5000 rpm are shown in increments of 100.
TIME hr:min During centrifugation, the display starts counting down when the
rotor begins to spin.
• Manual operation—indicates time remaining in the run.
• Programmed operation—indicates time remaining in the step.
The run automatically ends when the set time reaches zero (a tone
sounds when the rotor stops spinning). If a malfunction shuts down
the ultracentrifuge during a run, the time counting stops and the time
remaining when the run stopped remains in the display. By comparing
this time with the original set time, you can determine when the run
ended.
TEMPERATURE °C Indicates rotor temperature in degrees C at thermoequilibrium. The
temperature is displayed to a tenth of a degree and is within ±2
degrees of the set temperature (after equilibration).
PROGRAM • During setup—when the [PROG] key is pressed a dash flashes in
this display, indicating that a program number needs to be entered.
• During centrifugation—the number of the program being run is
displayed.
SPEED rpm TIME hr:min TEMPERATURE°C PROGRAM STEP ACCEL DECEL

1-6
Description
STEP • During setup—when the [STEP] key is pressed a dash flashes in
this display, indicating which step is being created or modified.
• During centrifugation— the number of the step being run is
displayed.
ACCEL When the [ACCEL] key is pressed, a dash flashing in the display indi-
cates that an acceleration rate can be entered. (Ten acceleration rates
are available to protect specific gradient and sample-to-gradient inter-
faces.) If no number is entered, the instrument accelerates at the
maximum rate and the display remains blank.
DECEL When the [DECEL] key is pressed, a dash flashing in the display indi-
cates that a number representing the selected deceleration rate can be
entered. (Eleven deceleration rates are available to maintain optimum
separation.) If no number is entered, the instrument decelerates at the
maximum rate and the display remains blank.
User Messages
User messages appear on the control panel to assist you in properly
operating the instrument or to alert you to conditions requiring atten-
tion. These user messages are described in detail, together with
recommended actions, in Section 4, TROUBLESHOOTING.
Run-Parameter Keys
Run-parameter keys are used to enter specific run information as
follows.
[DOOR]Unlocks the door and vents the chamber. (The instrument will not
accept this command if the rotor is spinning.)
[SPEED] Pressed to enter run speed (using the keypad). When entering the set
speed, the last three digits in the display are fixed as zeros and cannot
be changed.
[TIME]Pressed to enter run time (using the keypad), up to 99 hours and
59 minutes.

1-7
Description
[TEMP]Pressed to enter run temperature (using the keypad), from 0 to 40°C.
If a new temperature is not entered, the instrument automatically uses
the last temperature entered. (Default temperature for a new ultracen-
trifuge is 25°C.)
Keypad The keypad is used to enter numerical run parameters, to enter or
recall a program number, and to select acceleration and/or decelera-
tion rates. In addition to keys 0 through 9, the keypad includes a [ce]
(clear entry) key and an [ENTER/DISPLAY] key.
[ACCEL]The acceleration time is the time it takes a rotor to reach 5000 rpm
from 0 rpm. At 5000 rpm, the rotor then accelerates at maximum
speed until it reaches set speed. The [ACCEL] key is used to select one
of the ten acceleration rates (see Table 3-1 in Section 3) provided to
protect the gradient and sample to-gradient interface.
[DECEL]The deceleration time is the time it takes a rotor to decelerate from
5000 to 0 rpm. From set speed to 5000 rpm, the rotor decelerates with
full dynamic braking. The [DECEL] key is used to select one of eleven
deceleration rates (see Table 3-1 in Section 3) provided to maintain
optimum separation.
[START]To begin a run, [ENTER/DISPLAY] must always be the last key pressed
before pressing [START]. Further, you must press [START] while the set
values are being displayed. If you wait beyond this time, the [START]
key will not activate, and you will need to press [ENTER/DISPLAY] and
[START] again. The green light above the [START] key flashes until the
vacuum reaches 500 microns. At this point, the rotor begins to spin
and the light remains on until the end of the run.
[STOP]Can be pressed at any time to terminate a run. The green light above
the key flashes while the rotor decelerates. An audio indicator sounds
when the rotor comes to a complete stop.
➠NOTE
If you press [START] and then decide to open the
chamber door, you must press [STOP] before
pressing [DOOR], even if the rotor is at rest.