Bruker ultraflex Series User manual

ultraflex Series
Cleaning Manual
Revision 2 (January 2013)

Legal and Regulatory Notices Bruker Daltonik GmbH
Legal and Regulatory Notices
Copyright © 2013
Bruker Daltonik GmbH
All other trademarks are the sole property of their respective owners.
All Rights Reserved
Reproduction, adaptation, or translation without prior written permission is prohibited,
except as allowed under the copyright laws.
Document History
ultraflex Series Cleaning Manual Revision 2 (January 2013)
Part number: # (8)273408
First edition: January 2011
Warranty
The information contained in this document is subject to change without notice.
Bruker Daltonik GmbH makes no warranty of any kind with regard to this material,
including, but not limited to, the implied warranties of merchantability and fitness for a
particular purpose.
Bruker Daltonik GmbH is not liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance or use of this
material.
Bruker Daltonik GmbH assumes no responsibility for the use or reliability of its software on
equipment that is not furnished by Bruker Daltonik GmbH.
Use of Trademarks
The names of actual companies and products mentioned herein may be the trademarks of
their respective owners.
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Bruker Daltonik GmbH Legal and Regulatory Notices
Limitations on Use
For Research Use Only (RUO). Not for use in diagnostic procedures.
Hyperlink Disclaimer
Bruker Daltonik GmbH makes no express warranty, neither written nor oral, and is neither
responsible nor liable for data or content from the linked Internet resources presented in
this document.
ultraflex Series Cleaning Manual Revision 2 Page 3 of 44

Warning Symbols Bruker Daltonik GmbH
Warning Symbols
This symbol may be used on warning labels attached to the instrument.
When you see this symbol, refer to the relevant operation or service manual
for the correct procedure referred to by that warning label.
A triangular symbol indicates a warning. The meanings of the symbols that may appear
alongside warnings in the documentation or on the instrument are as follows:
Toxic material Electric shock RF radiation
Dangerous surface tem-
peratures
Heavy object (danger to
hands) Heavy object (danger to feet)
Harmful vapors or fumes Fire Part can be ejected
Explosion UV radiation Corrosive chemicals
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Bruker Daltonik GmbH Warning Symbols
Sharp object Moving parts
Strong magnetic field.
Keep pacemakers and magnetic
data storage devices clear from
this area.
Cryo gas Biohazard Entrapment
Gas bottles Laser Suffocation
Warnings, Cautions and Notes
Warnings and cautions appear in this manual. These warnings and cautions will detail the
specific hazard, describe how to avoid it, and specify the possible consequences of not
heeding the warning or caution. Read all warnings and cautions carefully and observe
them at all times. Their format is:
WARNING
A ‘Warning’ message appears in the manual when failure to observe
instructions or precautions could result in death or injury. Symbols
depicting the nature of the specific hazard are also placed alongside
warnings (refer to symbols on the next page).
CAUTION A ‘Caution’ message is used when failure to observe instructions could result
in damage to equipment.
Note A ‘Note’ is used to give advice or additional information.
ultraflex Series Cleaning Manual Revision 2 Page 5 of 44

Table of Contents Bruker Daltonik GmbH
Table of Contents
Legal and Regulatory Notices 2
Warning Symbols 4
Table of Contents 6
Document History 8
1 Safety Instructions 9
1.1 General Hazards 9
1.2 Instrument Liability (Maintenance) 9
1.3 Qualified Expert Personnel 9
1.4 Appropriate Maintenance 9
1.5 Special Safety Instructions 10
1.6 Laser Safety 11
1.7 Source Heater 13
2 Introduction 15
2.1 LC-MALDI 15
2.2 Imaging 15
2.3 Tools and Materials Required 17
3 Switching Off and Opening the Instrument 19
3.1 Preparation 19
3.2 Switching Off the Instrument 19
3.3 Opening the Source Chamber 25
3.4 Components Inside the Source 26
4 Cleaning the Source 29
4.1 Cleaning Optical Components 30
4.1.1 Laser Mirror 30
4.1.2 Laser Lens 31
4.1.3 Mirror Plate 32
4.1.4 Camera Optics 33
4.1.5 Illumination Optics 33
4.2 Cleaning Potential Plates 34
4.3 Final Tasks 37
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Bruker Daltonik GmbH Table of Contents
5 Closing and Switching On the Instrument 39
5.1 Check HV Connections 39
5.2 Closing the Source 40
5.3 Switching On the Instrument 42
ultraflex Series Cleaning Manual Revision 2 Page 7 of 44

Document History Bruker Daltonik GmbH
Document History
Revision Date Changes Approval
1 03.01.2011 First Edition S. Hasemann
2 11.01.2013 Add laser safety information S. Hasemann
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Bruker Daltonik GmbH 1 Safety Instructions
1 Safety Instructions
1.1 General Hazards
The following safety instructions serve to protect the life, body, and health of third parties
and to prevent damage to the instrument components. These safety instructions must be
taken into consideration to avoid hazards that can result from inappropriate equipment
maintenance. Bruker accepts no responsibility for damage resulting directly or indirectly
from inappropriate maintenance work.
1.2 Instrument Liability (Maintenance)
On the customer's premises, it is the obligation of the person nominated as being
responsible for equipment maintenance (the responsible person), to ensure that
equipment maintenance is only undertaken in line with the entirety of the safety
instructions. In addition, the responsible person must ensure that the Maintenance
Instructions to be used by the person entrusted with equipment maintenance are available
in a complete and legible form before and during the maintenance process.
1.3 Qualified Expert Personnel
To ensure safe working and the avoidance of hazards to the customer, all maintenance
work must be conducted by a Bruker Service representative or by a suitably qualified
specialist who has carefully read and fully understood the Maintenance Instructions before
undertaking the work.
1.4 Appropriate Maintenance
A maintenance schedule can only be appropriately undertaken if it strictly follows an
approach that takes all safety considerations described in the Maintenance Instructions
into account. Ignoring safety considerations or deviating from the written procedure is
considered inappropriate maintenance work, for which Bruker accepts no
responsibility(see section 1.1).
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1 Safety Instructions Bruker Daltonik GmbH
1.5 Special Safety Instructions
WARNING — LASERRADIATION
When all covers in place, users are protected from exposure to laser
light (Class I Laser Product).Operating the instrument with opened
covers can expose anyone in the area of the instrument to harmful
laser light, which may result in blindness. Protective glasses must
always be worn when the covers are open and the instrument is
switched on.
WARNING — DANGEROFCHEMICALBURNS
Depending on the use to which the instrument has
been put, the cleaning process may expose the user
to harmful chemicals. Wear appropriate eye
protection and gloves. Read the Material Safety Data
Sheets (MSDS) available from the material
suppliers.
WARNING — DANGEROFELECTRICSHOCK
When the protective covers are removed from the instrument and
the instrument is connected to the electrical supply, there is a danger
of electric shock. Take appropriate precautions when working on an
unguarded instrument.
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Bruker Daltonik GmbH 1 Safety Instructions
WARNING — BIOLOGICALHAZARD
Depending on the use to which the instrument has been put,
the cleaning process may expose biological substances. These
substances may be infectious and/or harmful. Take
appropriate precautions and use appropriate safety
equipment. Refer to local biohazard regulations and consult
the Safety Representative before starting work.
WARNING — SLIP HAZARD
Accidental spills of cleaning liquids can cause a slip hazard. Ensure that
all spills are cleaned up immediately.
Note Always allow sufficient time for maintenance procedures. As a guide, 2 hours is
the minimum that should be allowed.
Note Make sure that a ready supply of absorbent material (such as paper towels) is
available to clean up spills.
1.6 Laser Safety
WARNING
This instrument contains a Laser class 4 product option.The
enclosure surrounding the instrument is designed to protect the
user from exposure to the invisible radiation. Operating the
instrument with opened covers can expose the user to harmful
laser radiation, which may result in blindness.
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1 Safety Instructions Bruker Daltonik GmbH
Figure 1-1 Labels on the smartbeam II laser inside the instrument
For further information about the smartbeam II laser system, refer to Holle, A; Haase, A;
Kayser, M; & Höhndorf, J. Optimizing UV laser focus profiles for improved MALDI
performance, Journal of Mass Spectrometry, Vol 41, Issue 6. doi:10.1002/jms.1041.
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Bruker Daltonik GmbH 1 Safety Instructions
1.7 Source Heater
Figure 1-2 Source heater warning label
Note Because the instrument will be disconnected from the power supply, there is no
risk from laser light if the working steps and instructions given in this manual are
followed correctly.
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1 Safety Instructions Bruker Daltonik GmbH
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Bruker Daltonik GmbH 2 Introduction
2 Introduction
Because different applications lead to differing levels of contamination, the manual and the
source heating cleaning intervals can vary depending on the applications performed on the
instrument.
Note The source heating cleaning interval can be set under flexService > Laser
and Trigger. This interval must be set by qualified service personnel.
2.1 LC-MALDI
Source heating interval
lCleaningthreshold = 5 million shots (default) as shown in Figure 2-1.
Manual cleaning interval
lWeekly — Flushing
lMonthly — Clean the optical components using a swab (see section 4.1). Use a
brush to clean the potential plates (see section 4.2)
2.2 Imaging
Source heating interval
lCleaningthreshold =1 million shots as shown in Figure 2-2.
Manual cleaning interval
lAfter 10 million shots and after a large image — Clean the laser optics using a swab
(see section 4.1). Use a brush to clean the potential plates (see section 4.2)
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2 Introduction Bruker Daltonik GmbH
Figure 2-1 Cleaning Information
dialog settings (LC-
MALDI)
Figure 2-2 Cleaning Information
dialog settings (MALDI
imaging)
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Bruker Daltonik GmbH 2 Introduction
2.3 Tools and Materials Required
Item Description
Key For instrument key-switch and to acti-
vate top cover lift (delivered with the
instrument).
Allen key 5 mm For loosening and removing source
door screws.
HPLC- grade ethanol
(approx. 200 mL)
For cleaning source parts
Safety wash bottle For example, VITLAB, VITsafe,
narrow mouth, 500 ml PELD (Bruker
#(8)201106).
Safety glasses Safety glasses with side protection.
Tissues Free of any impregnation (for exam-
ple, Kimberly- Clark KIMTECH
SCIENCE Laboratory Tissues;
Bruker #(8)321559
Swab (for cleaning
optics only)
Lint- free; not a Q- tip (cotton bud).
For example, Anti- Static swab
TX761D, from basan (Bruker
#(8)270758
Brush (for cleaning
metal parts only)
Bruker # 228209
Nitrile gloves For example, KIMBERLY- CLARK
PURPLE NITRILE Exam Gloves
(Bruker #(8)267458)
Compressed clean gas
(nitrogen)
For ventilating the system and drying
components
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2 Introduction Bruker Daltonik GmbH
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Bruker Daltonik GmbH 3 Switching Off and Opening the Instrument
3 Switching Off and Opening the Instrument
3.1 Preparation
lEject / remove the MALDI target plate to avoid damaging your samples.
lMove the sample holder to position A24 to enable better access to the P1-cable
contact pole.
lClose flexControl and flexService .
lThe external nitrogen supply must be opened to allow ventilation of the vacuum
system.
3.2 Switching Off the Instrument
Figure 3-1 ultrafleXtreme— front view
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3 Switching Off and Opening the Instrument Bruker Daltonik GmbH
Figure 3-2 Control panel — instrument switched on, key is vertical
lSwitch off the instrument at the key-switch on the control panel at the front of
the ultraflex.
oTurn the key one quarter turn counterclockwise to the horizontal position.
oThe pumps, the high voltage, and the lasers are now switched off.
Figure 3-3 Control panel — instrument switched off, key is horizontal
Figure 3-4 Control panel — key removed
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