Drawell DW-T960 User manual

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Product Specifications for PCR
Suitable for T960
Please read the manual before installation and operation
Drawell International Technology Limited
Chongqing Drawell Instrument CO,.Ltd
Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District,
Chongqing,China
Tel: [phone]
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1117,Lane561 XiuChuan Rd.,PuDong New Area,Shanghai,China
Web : www.drawell.com.cn
Email : [e-mail]

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Contents
2 Chapter 1 Important Notice
2 1. Unpacking check
2 2. Safety notice
5 Chapter 2 Basic Functions
5 1. Structure of T960
6 2. Lid-opening angle
6 3. Heat Lid positions
7 4. Module
10 5. Touch Screen
10 6. Working condition and performance indicator
12 Chapter 3 Instructions
12 1. Program installation
12 2. Startup interface
13 3. Files
13 4. Setting
14 5. Heat Lid
14 6. Gradient
15 7. Apply
16 8. Log
16 9. System
17 10. Help
18 Chapter 4 Programming
18 1. Create files
21 2. Open files
23 Appendix A Maintenance and troubleshooting
23 1. Maintenance
23 2. Troubleshooting
25 Appendix B Instrument Guarantee
25 1. Guarantee Content
25 2. Guarantee Scope

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Notice: If you find the package seriously broken or the instrument
obviously damaged after receiving the instrument, please keep the
original package and contact your distributor as soon as possible so
we can change a new one for you.
Please read the manual carefully before operating the instrument,
the wrong operation may be hazardous to the operator or the
instruments itself.
Chapter 1 Important Notice
1 Unpacking check
Upon receiving B960 gradient thermal cycler, please unpack the box to confirm if
the following items are contained or not:
① One thermal cycler (including one sample block)
② One power cord
③ One user manual
④ One warranty card
5One QC certificate
6One packing list
7One instruments test report
8Two spare fuses (AC250V/10A)
9Other optional items according to the contract
10 Mouse, Disk
If the contents do not comply with the packing list, please contact the distributor as
soon as possible.
2 Safety notice

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① Operating safety
The power cord for the instrument should be grounded reliably. The instrument
adopts three-core grounding plug, and should be connected with appropriate
power socket. Before connecting to the power, you must make sure that the
voltage and power load of the socket is in accordance with the capacity on the
instrument's nameplate.
Please change the power cord with the same type if the skin of the cord is found to
be broken. Nothing is allowed to be place on the power cord and the cord shall
not be placed in the area that may be easy approaching by people. Be sure to hold
the plug tightly while inserting, pulling of the cord directly is forbidden.
While the instrument is in operation, the sample plate block and the heat lid will
produce the high temperature, so no part of human body is allowed to touch the
metal block or the heat lid while the instrument is in operation in case of any
scalding.
For the good ventilation, no articles are allowed to be placed within 30cm around
the instrument.
②Maintenance Safety
The block and the heat lid of the instrument need regular cleaning to guarantee
the precision of the experiment. Before cleaning, the sample plate block is suggested
to be taken out for soft scrubbing by the clean soft cloth stained with the pure alcohol
in appropriate amount. No corrosive cleaning agent is allowed for use and dripping
the cleaning agent into the taper hole is forbidden in case of any damage to the
instrument. And the sample plate block should be put back into the instrument after
cleaning.
Please cut off the power at once and stop the experiment and then immediately
contact the distributor or the professional maintenance personnel in case of any
occurrence of the following situations:
Any reagent, rain water or other liquid enters into the instrument.
The instrument falls from a high place or the instrument shell is damaged.
The operating instrument produces the abnormal noise or the pungent odor.
The features of the instrument change apparently, such as failures of startup or
shutdown or failure to run user program, etc.

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Notice: For your personal safety and the normal operation of the
instrument, please do not disassemble the instrument by yourself.
Any such behavior may endanger your safety and deprive your
guarantee right of the instrument.
③Trial time tips
The above figure indicates that the remaining trial time is 91 hours.
④ How to get the encryption key after the trial period
expires
When the trial period is about to end, please contact the merchant or
manufacturer to get the encryption key, to facilitate you to continue to use.

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⑶
⑴
⑷
⑵
⑸
⑺
⑻
⑹
⒂
⒃
⒄
⑼ ⑽ ⑾⑿⒀⒁
⒅
⒇
⒆
Chapter 2 Basic Functions
2.1 Structure of T960
(1) Ventilation window (2) Base (3) Foot rest (4) Ventilation window (5) Upper lid (6)LCD
panel (7)LCD Screen (8)Aluminum panel (9) Power switch (10) Power socket (11) Fuse
socket (12) Network interface (13) USB interface (14) JTEG interface (15) Shell (16)
Ventilation window (17) Long label (18) Lid wrench (19) Block selecting knob (20) Block
selecting indicator

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Pull the wrench to
push the lid
backward
Stop at any angle
according to
requirements
Notice: According to the instrument characteristics, the open angle scope
between upper lid and the shell is 0°~ 90°, please keep it within the scope to
prevent any damage to the instrument.
2.2 Lid-opening angle
This type of instrument takes a leading role in adopting the hinge joint
technology, enabling the upper lid of the instrument to stay at any angle according
to requirement and solving such problems brought by the traditional method of
relying on spring elasticity to open the lid as unreliability, instability, large inertia and
short life etc.
As indicated in the above two figures, when the wrench is raised the buckle will be
released, and then the user can set the upper lid at any angle according to their
requirement. Humanistic structure endows the product more beautiful and practical
characteristics, and guarantees the safety and convenient operation.
2.3 Heat Lid positions
Frequently, experiments need different reaction tubes, that is, use different blocks
to match the tubes. The design of the heat lid gear positions realize the heat lid to
match different sample plate blocks just by turning the knob to the appropriate
position.

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Notice: According to characteristics of the instrument, the turning range of the
switch is between 0.5 to 384, do not try to turn the switch out of this range.
Block components
As the above figures indicate that the mark of 0.5, 0.2,384 (clockwise) on the panel
respectively corresponds to the gear position of tube 0.5ml, 0.2ml and 384sample plate
blocks. If you need the 0.2ml tube for experiment, you only need to change the block
with 0.2ml block (See 2.4 Module for details), then switch the gear to the mark of 0.2,
and then you can start your experiment. The design abandons the traditional practice
of changing heat lid/or even instrument.
2.4 Module
For the need of users to use tubes of different specifications for experiment in the
same instrument, and for the convenience of users to change relevant parts directly
and effectively, the instrument adopts the portable design with auto-lock function to
make the instrument meet the experiments of different tubes on the condition of
changing least parts, which fully reflects the humanistic design of the instrument.
The above figure indicates that the integrated block parts design of the instrument
greatly improves operational convenience no matter in installation or replacement,
and the overall dimension, assembly process and installation method of all the blocks
of all specifications are the same (The block parts of different specifications differ with
blocks corresponding to tubes of different specifications). And this guarantees that
users can change or install the block in a fast and high-efficient way only by choosing

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the block with specification needed.
Figure 1 show how to change the block unit. When changing the block, the user
shall pull the handle upward, when the handle and the shell are in a vertical state, the
block will be unlocked, and then the user can easily take out the block part by pulling
the handle vertically. Figure 2 show how to insert the block, when the user confirm the
block part of certain specifications needed, the user only needs to hold the block’s
handle. Vertically insert the block in position, then put the handle down to complete
the operation.
Step 1: Move the handle to the state vertical to the shell to make the block parts
unlocked. (Figure 3)
Step 2: Move the block parts upward slightly, and lay it in a safe place after the block
parts are completely off the shell. (Figure 4)

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Notice: Since all the lines of the instrument are connected by connectors, the
user shall use proportional strength while taking out or installing block parts,
and use skilled strength to shake them left and right. No tough strength is
allowed in case of any damage to connectors.
Step 3: Choose the block parts of specifications needed and gently put them into the
shell vertically.
Step 4: After the block parts completely contact the shell, press back the handle down.
(Figure 6)
Step 5: When the block parts and the shell will lock tight. Till now the replacement of
block parts is over. (Figure 7)
The integrated block parts coupled with the intelligent block auto-lock mechanism
enable the user to conduct replacement in a faster and safer way. In addition, the
option of block parts of various specifications enable the users to conduct experiments
with tubes of different specifications in one set of instrument.

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Notice: In order to guarantee the normal use of the instrument and the
accuracy of experimental results, please run or store the instrument according
to prescribed working conditions and storage conditions. Or the wrong
operation will damage the instrument and even personal safety.
2.5
Touch screen
This machine is the latest touch-screen model，it has characteristics of convenient
operation，powerful function，high sensitivity, beautiful appearance and so on.
2.6 Working condition and performance indicator
2.6.1 Instrument working condition
Ambient temperature : 10℃～30℃
Relative humidity: ≤ 70%
Power supply: AC220V±22V 250VA 50Hz±1Hz
2.6.2 Instrument storage condition
Ambient temperature: -20℃~55℃
Relative humidity: ≤80%

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2.6.3 Instrument performance indicator
Model: T960-A；B960-B；B960-C；B960-D
Capacity: 96*0.2mL(A);54*0.5mL(B);96*0.2mL+77*0.5mL(C);384well(D)
Temp range: 0℃~99.9℃（Rt≤30℃）
Max heating rate: ≥5℃/s
Max cooling rate: ≥5℃/s
Uniformity:≤±0.2℃(Constant 10s)
Accuracy:≤±0.1℃
Gradient temp range: 30℃~99℃
Gradient spread: 1℃~30℃
Heat lid temp: 20℃~110℃
Temp control: block,tube ,calculated
Stored program number: 2000(The External U disk unlimited)
Maximum number of cycles: 999
Display: 7 inch LCD
Communication: USB2.0、RS232、RJ45
Size: 380mm(L)*270mm(W)*250mm(H)
Weight: 8.1kg

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3-2
Chapter 3 Instructions
3.1 Program installation
Step1. Install the ActiveSync software in your PC.
Step2. Connect the PC and PCR machine with USB cable.
Step3. Right-click “my computer” and select properties€”Hardware”€”Device
Manager” €”Port” (COM&LPT) €”Communication Port”(COM1) ,then
install the COM1 communication port.
Step4. Click “scan” to choose the CD-ROM directory.
Step5. In the “New Partnership” window choose “No”. Then it shows the PC and
the PCR machine is properly connected.
Step6. Select the “NandFlash” in the PC.
Step7. In the “NandFlash”, replace the old version “TCtrl” with the new one, then
updated successfully.
3.2 Startup interface
After connecting related power, turn on the power switch of the instrument and it
will produces the sound of “tick-tack”, which means the instrument is in operation,
and now the screen will display the startup interface (Figure 3-1), then the instrument
will start automatic systematic inspection, and then enter the stand-by interface
(Figure 3-2) if no fault is found. If so, this means the instrument is free for experimental
operations now. If the screen is dark or any other abnormal phenomena occur while
turn on the instrument, please cut off the power immediately and refer to Chapter 5 for
maintenance or contact the distributor or the supplier for settlement.
3-1

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3-2
3.3 Files
In the" Main Menu" select "File" submenu to get into the "File" interface, then you
can choose to click the "Delete file " or "Create file".(Figure 3-3)
3-3
Notice: "Create file” -- Create new files.
"Delete file” -- Delete files.
Specific program file setting showed in chapter 4.
3.4 Setting
In the" Main Menu" select "Set" submenu to get into the "Operation Parameters
Settings" interface. (Figure 3-4)

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3-4
Notice: The up/down rate scope is 0.0 ℃/s～ 5.0 ℃ /s. The temperature scope of low
temperature preservation is 0.0℃～40.0℃.Temperature control mode: sample
block control and tube control.
3.5 Heat Lid
In the "Main Menu" select "Lid" submenu to get into the "Heat Lid Function
Settings" interface. (Figure 3-5)
3-5
Notice:The Heat lid temperature scope is: 20℃～110℃.
3.6 Gradient
In the" Main Menu" select “Grads" submenu to get into the "Gradient View"
interface. (Figure 3-6)

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3-6
Notice: 1.Central temperature range:31℃～99℃, grads range:1℃～15℃,the algebra
sum of the central temperature and the grads scale range:30℃～100
℃.Operator can view the temperature of 12 corresponding block rows.
2.
The Gradient graph cannot used if user put in the 384module since the
gradient function of the 384module is not available.
3. The colour of the gradient figured above, don’t refer to the temperature
and the colour of the block, it is the position of every line.
3.7 Apply
In the" Main Menu" select "apply" submenu to get into the "Appointment and
Alarm Settings" interface. (Figure 3-7)
3-7

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3.8 Log
In the "Main Menu" Select "Log" submenu to get into "Log View" interface. (Figure
3-8)
3-8
Notice: If the numbers of all currently created files is below 2000, all the files
information will be displayed in this window. If the number of files is above
2000, the most recently created 2000 files’ information will be displayed. This
information include the file names, running time, and created date, etc.
3.9 System
In the" Main Menu" select "System" submenu to get into the "System Settings"
interface. (Figure 3-9)
3-9
Notice: 1.Press the numerical keys on the soft keyboard to set the system date
and Time.
2.
Press the “Apply” key to modify the system date, alert sound, file list
arrangement type, etc.
3.
Insert the U disk into your machine,then press the "Export"
button,the user's program files will be copied to the "jgFolder" folder in
the U disk.

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4. Insert the U disk into your machine,then press the "Import"button,all
of the user's program files that is copied to the "jgFolder"folder in step
3 will be imported into the application program
3.10 Help
In the "Main Menu" select "Help" submenu to get into the "Help" interface. (Figure
3-10)
3-10
Notice: 1 . The “TM” key showed on the interface is for checking the TM value. If
the user inputs the value forbidden by the system while setting the parameter,
the system will choose the default value, and the forbidden value will not be
allowed.
2. When the trial period is about to end，click the "unlock" key to get a
verification code，then contact the merchant or manufacturer to obtain the
corresponding encryption key.

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Notice: Select the “Close” key at any time the system will back to the
Windows CE system interface.
Chapter4 Programming
4.1 Create Files (The following pictures are just for reference only)
The following is a sample(30 cycles, 95 ℃ :45S ， 55 ℃ :45s ， 72 ℃ :45S) of how to
program. (Figure 4-1)
Temp Edit Pull-down Menu
Cycle Edit Pull-down Menu
Running Key
Right/Left selection key
“Close” key
“Back” key
4-1
Step 1：Open the "File" menu ,then input the file name，to search the file that you want
to use (Figure 4-2). Also，you can click the "Create File" key，then input the file
name and password（If you need），click the "Confirm" key to confirm after
closing the small keyboard(Figure 4-3).
4-2

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4-3
Step 2: Get into the "Temp Edit " and "Cycle Edit" interface for editing, click the "Temp "
pull-down menu and choose the "Edit Temp Point" to edit temperature. If you
need to increase temperature, click the "Temp " pull-down menu and select the
"Add Temp Point" for editing. Select the "Delete Temp Point” to remove temp
point if there is extra point. (Figure 4-4)
4-4
Step 3: In the "Temp Edit”and" Cycle Edit" interface, use the“Right/Left selection”
key or“Arrow”keys to move the cursor to the temp point which will be the
temp cycle starting point, click the "Cycle " and select the "Cycle Start Point”.
Then move to the one which will be the cycle ending point, click the "Edit Cycle
" and select the "Cycle End Point". (Figure 4-5)
Notice: if you want to delete the finished cycle, you can choose the temperature
point in the cycle, then click “cycle edit” pull down frame ”cycle edit”,
change the number to 0 and then click ok.