Endress+Hauser analytikjena Biometra TOne User manual

Operating Manual
Biometra TOne
High-performance thermal cycler for
DNA amplification by PCR

Biometra GmbH (Part of Analytik Jena GmbH)
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6-0
Fax + 49 551 50 68 6-66
Email [e-mail]
Biometra GmbH
Rudolf-Wissell-Str. 15
37079 Göttingen Germany
Phone + 49 3641 777407
Email [e-mail]
http://www.analytik-jena.com
Operating manual valid from software version ME 1.70 – RE 1.46
Follow these instructions for proper and safe use. Keep for future reference.
34-3800-000-23-e
B (11/2021)
Analytik Jena GmbH
© Copyright 2021, Analytik Jena GmbH
Manufacturer
Service
General information
Documentation number
Edition
Implementation of the
Technical Documentation

Biometra TOne Contents
3
Contents
1Basic information ....................................................................................................... 7
1.1 Notes on the operating manual........................................................................................ 7
1.2 Purpose.............................................................................................................................. 7
2Safety and warning instructions ................................................................................ 9
2.1 General notes .................................................................................................................... 9
2.2 Safety markings on the Biometra TOne ........................................................................... 9
2.3 Technical condition ......................................................................................................... 10
2.4 Requirements for the operating personnel.................................................................... 10
2.5 Safety instructions: transport and installation ............................................................... 10
2.6 Safety instructions: during operation ............................................................................. 11
2.6.1 General ............................................................................................................................ 11
2.6.2 Safety instructions: protection against explosion and fire............................................. 11
2.6.3 Safety instructions: electrical equipment ....................................................................... 11
2.6.4 Handling of samples, auxiliary and operating materials ............................................... 12
2.7 Safety instructions: service and repair............................................................................ 12
2.8 Behavior during emergencies ......................................................................................... 13
3Function and setup................................................................................................... 14
3.1 High speed thermoblock ................................................................................................. 14
3.2 High Performance Smart Lid .......................................................................................... 15
3.3 Display ............................................................................................................................. 15
3.4 User-specific quick start of programs ............................................................................. 15
3.5 Easy programming .......................................................................................................... 16
3.6 Linear Gradient Tool ....................................................................................................... 16
3.7 GLP compliance............................................................................................................... 16
3.8 Open system philosophy ................................................................................................. 16
3.9 Order numbers ................................................................................................................ 17
4Installation ............................................................................................................... 18
4.1 Scope of delivery ............................................................................................................. 18
4.2 Unpacking and inspection............................................................................................... 18
4.3 Setting the operating voltage ......................................................................................... 18
4.4 Commissioning................................................................................................................ 19
4.4.1 General setup .................................................................................................................. 19
4.4.2 Installation when used in a network .............................................................................. 19
5Control elements ...................................................................................................... 21
5.1 Biometra TOne front view .............................................................................................. 21
5.2 Biometra TOne rear view................................................................................................ 21
5.3 High Performance Smart Lid (HPSL).............................................................................. 22
5.3.1 Closing the lid.................................................................................................................. 22
5.3.2 Opening the lid................................................................................................................ 23
5.4 The Biometra TOne software ......................................................................................... 23
5.4.1 Keyboard.......................................................................................................................... 23
5.4.2 Keypad............................................................................................................................. 24
5.4.3 Cursor keys ...................................................................................................................... 25
5.4.4 Confirm or Cancel............................................................................................................ 25
5.4.5 List of commands and icons used................................................................................... 25
6Operation.................................................................................................................. 30
6.1 Power on self-test ........................................................................................................... 30
6.2 Login screen .................................................................................................................... 30
6.3 Login................................................................................................................................ 31
6.4 Home screen.................................................................................................................... 32

Contents Biometra TOne
4
6.5 Block status...................................................................................................................... 33
6.6 Quick start function......................................................................................................... 34
6.7 Incubation mode ............................................................................................................. 35
7Creating, editing and saving programs.................................................................... 37
7.1 Programming modes....................................................................................................... 37
7.2 Creating a new program/using a program template ..................................................... 38
7.3 Assigning program names .............................................................................................. 40
7.4 Setting the heated lid temperature ................................................................................ 40
7.5 Preheating the heated lid ............................................................................................... 41
7.6 Editing steps .................................................................................................................... 41
7.6.1 Editing all parameters of a step...................................................................................... 42
7.6.1.1 Programming loops......................................................................................................... 43
7.6.1.2 Programming a temperature increment......................................................................... 44
7.6.1.3 Programming a time increment...................................................................................... 44
7.6.1.4 Adjusting the ramp rate.................................................................................................. 45
7.6.1.5 Programming a gradient step......................................................................................... 45
7.6.2 Direct programming of program parameters for a step................................................. 47
7.7 Inserting a step................................................................................................................ 50
7.8 Deleting a step................................................................................................................. 50
7.9 Saving the program......................................................................................................... 50
8Starting, coping and deleting programs .................................................................. 53
8.1 Starting a program .......................................................................................................... 53
8.2 Copying a program .......................................................................................................... 54
8.3 Copying all programs....................................................................................................... 55
8.4 Deleting a program ......................................................................................................... 56
8.5 Deleting all programs...................................................................................................... 57
9Running, pausing, continuing and stopping programs ........................................... 58
9.1 Display during a run ........................................................................................................ 58
9.2 Pausing a program .......................................................................................................... 59
9.3 Continuing the program.................................................................................................. 61
9.4 Skipping a step ................................................................................................................ 61
9.5 Stopping the program ..................................................................................................... 61
10 Tools ......................................................................................................................... 62
10.1 Configuration................................................................................................................... 62
10.1.1 Setting the date and time ............................................................................................... 63
10.1.2 Automatic user logout..................................................................................................... 64
10.1.3 Configuring the beeper ................................................................................................... 65
10.1.4 Network ........................................................................................................................... 65
10.1.4.1 Network settings ............................................................................................................. 65
10.1.4.2 Network users.................................................................................................................. 66
10.1.5 Display brightness ........................................................................................................... 66
10.1.6 Screen calibration............................................................................................................ 67
10.1.7 Factory settings ............................................................................................................... 67
10.2 User management........................................................................................................... 68
10.2.1 Creating a user................................................................................................................. 69
10.2.2 Editing user settings........................................................................................................ 69
10.2.3 Delete user....................................................................................................................... 70
10.3 Documentation................................................................................................................ 71
10.3.1 Run log file....................................................................................................................... 71
10.3.2 Power On Logfile............................................................................................................. 74
10.3.3 Extended self-test log file ............................................................................................... 75
10.3.4 Error log file..................................................................................................................... 75
10.4 Extended self-test ........................................................................................................... 76
10.5 Service info file (SINF)..................................................................................................... 78

Biometra TOne Contents
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10.6 Backup ............................................................................................................................. 79
10.7 Cycler Info........................................................................................................................ 80
10.8 Contact............................................................................................................................. 81
11 Adaptation of programs ........................................................................................... 83
12 Quick reference guide............................................................................................... 85
13 Troubleshooting ....................................................................................................... 90
13.1 Administrator password forgotten ................................................................................. 90
13.2 Slow heating and cooling................................................................................................ 90
13.3 Auto restart ..................................................................................................................... 90
13.4 Auto restart without an apparent cause......................................................................... 90
13.5 Adaptation of programs from other thermal cyclers ..................................................... 91
13.6 Loosening the lid wheel in case of blockage .................................................................. 91
14 Maintenance and care.............................................................................................. 92
14.1 Cleaning the housing ...................................................................................................... 92
14.2 Disinfecting the device.................................................................................................... 93
14.3 Firmware update............................................................................................................. 93
15 Service ...................................................................................................................... 94
15.1 Device return information............................................................................................... 94
15.2 Packing the Biometra TOne............................................................................................ 95
16 Disposal .................................................................................................................... 96
17 Technical specifications............................................................................................ 97
18 Declaration of conformity ........................................................................................ 99
19 Index.......................................................................................................................100
Figures
Fig. 1 The Biometra TOne Thermal Cycler ............................................................................... 14
Fig. 2 Voltage selector switch on the underside of the device................................................. 18
Fig. 3 Front view of the Biometra TOne................................................................................... 21
Fig. 4 Rear view of the Biometra TOne .................................................................................... 22
Fig. 5 Placement of sample tubes in the block......................................................................... 23
Fig. 6 Keyboard.......................................................................................................................... 24
Fig. 7 Keypad............................................................................................................................. 24
Fig. 8 Start screen...................................................................................................................... 30
Fig. 9 Login screen .................................................................................................................... 31
Fig. 10 Select user screen............................................................................................................ 31
Fig. 11 Enter password screen .................................................................................................... 32
Fig. 12 Home screen.................................................................................................................... 32
Fig. 13 Bock status/quick start button........................................................................................ 33
Fig. 14 Biometra TOne program quick start screen ................................................................... 35
Fig. 15 Biometra TOne incubation mode screen........................................................................ 36
Fig. 16 Spreadsheet programming ............................................................................................. 37
Fig. 17 Graphical programming.................................................................................................. 37
Fig. 18 Program overview screen................................................................................................ 39
Fig. 19 Blank program template................................................................................................. 39
Fig. 20 Program template screen ............................................................................................... 40
Fig. 21 Heated lid temperature setting screen........................................................................... 41
Fig. 22 Pprogram step editing screen......................................................................................... 43

Figures Biometra TOne
6
Fig. 23 Temperature gradient on a 96 well sample block.......................................................... 45
Fig. 24 Linear Gradient Tool screen............................................................................................ 46
Fig. 25 Standard gradient programming screen......................................................................... 47
Fig. 26 Biometra TOne spreadsheet program view.................................................................... 47
Fig. 27 Gaphical program view.................................................................................................... 48
Fig. 28 Individual program parameter editing............................................................................ 49
Fig. 29 Gradient view .................................................................................................................. 49
Fig. 30 Program overview screen................................................................................................ 51
Fig. 31 Quick select screen .......................................................................................................... 52
Fig. 32 Program overview screen................................................................................................ 53
Fig. 33 Biometra TOne program overview screen with preview................................................ 54
Fig. 34 Program overview screen................................................................................................ 55
Fig. 35 Program overview screen................................................................................................ 56
Fig. 36 Confirmation prompt....................................................................................................... 56
Fig. 37 Confirmation prompt....................................................................................................... 57
Fig. 38 Displays during the run in a graphical, spreadsheet and gradient view........................ 59
Fig. 39 Run display screens during a program pause................................................................. 60
Fig. 40 Confirmation prompt....................................................................................................... 61
Fig. 41 Biometra TOne tools screen............................................................................................ 62
Fig. 42 Settings screen ................................................................................................................ 63
Fig. 43 Date and time entry screen............................................................................................. 63
Fig. 44 Automatic user logout screen ......................................................................................... 64
Fig. 45 Beeper configuration screen ........................................................................................... 65
Fig. 46 Network settings screen.................................................................................................. 66
Fig. 47 Display brightness adjustment screen ............................................................................ 67
Fig. 48 Calibration screen............................................................................................................ 67
Fig. 49 Confirmation prompt....................................................................................................... 68
Fig. 50 Biometra TOne user management screen...................................................................... 68
Fig. 51 Biometra TOne create new user screen.......................................................................... 69
Fig. 52 User data screen.............................................................................................................. 70
Fig. 53 Confirmation prompt....................................................................................................... 70
Fig. 54 Biometra TOne documentation screen........................................................................... 71
Fig. 55 Biometra TOne run log file overview screen .................................................................. 72
Fig. 56 Biometra TOne run log file screen ........................................................................................ 73
Fig. 57 Program view screen....................................................................................................... 74
Fig. 58 Power on log file screen.................................................................................................. 75
Fig. 59 Biometra TOne extended self-test log file screen.......................................................... 75
Fig. 60 Biometra TOne error log file screen ............................................................................... 76
Fig. 61 How to load the sample block with plasticware for the extended self-test ..................77
Fig. 62 Biometra TOne extended self-test screen ............................................................................ 77
Fig. 63 Extended self-test overview screen ................................................................................ 78
Fig. 64 Service info file creation screen ...................................................................................... 79
Fig. 65 Backup file screen............................................................................................................ 80
Fig. 66Cycler Info screen............................................................................................................. 81
Fig. 67 Service department contact details screen ..................................................................... 82

Biometra TOne Basic information
7
1Basic information
1.1 Notes on the operating manual
The Biometra TOne is intended for operation by qualified specialist personnel observing
this operating manual.
The operating manual contains information about the design and operation of the Bi-
ometra TOne and provides personnel familiar with PCR technology with the necessary
know-how for the safe handling of the equipment and its components. The operating
manual further includes notes on the maintenance and service of the device and poten-
tial causes and remedies of any faults.
Instructions for action which occur in chronological order are numbered and combined
into action units and furnished with the corresponding results.
Lists which are not in chronological order are shown as itemized lists, sub-listings as
bullet points.
Safety instructions are indicated by pictographs and signal words. The type and source
of the danger are stated together with notes on preventing the danger.
The operating manual uses the following symbols and signal words to indicate hazards
or instructions. The safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation which might cause fatal or very serious inju-
ries (deformities).
CAUTION
Indicates a potentially hazardous situation which might cause light or minor injuries.
NOTICE
Provides
indications of potential material and environmental damage.
1.2 Purpose
The Biometra TOne is an end-point thermal cycler. It is designed to amplify nucleic acids
by repeated cycles of heating and cooling using DNA polymerases in a PCR reaction.
The thermal cycler is developed as product for general laboratory use.
The Tone combines modern design with user-friendly software. The user interface con-
sists of a touchscreen with a graphical or spreadsheet display that shows the time, the
status and the temperature program for each run. Using the touchscreen keyboard,
Conventions
Symbols and signal words
used

Basic information Biometra TOne
8
information and program parameters can be entered directly on the screen. Due to the
high ramp rates, the TOne thermal cycler is suitable for fast PCR applications, thereby
helping to shorten program run times.

Biometra TOne Safety and warning instructions
9
2Safety and warning instructions
2.1 General notes
For your own safety and to ensure error-free and safe operation of the Biometra TOne,
please read this section carefully before commissioning.
Besides the safety instructions in this user manual and the local safety regulations that
apply to the operation of the device, the general applicable regulations regarding acci-
dent prevention, occupational health and safety and environmental protection have to
be observed and complied with.
References to potential dangers do not replace the work protection regulations which
must be observed.
2.2 Safety markings on the Biometra TOne
Warning and instruction signs are attached to the device, the meaning of which must be
observed.
Damaged or missing warning and instruction signs can lead to incorrect actions with
personal injury and material damage. The signs must not be removed. Damaged warn-
ing and instruction signs must be replaced immediately!
The following warning and instruction signs are attached to the device:
Warning signs Meaning Notice
Warning of danger place Proceed with due care when handling sam-
ples and working with the Biometra TOne.
Warning against hot
surface
There is a risk of burning at the heated lid,
the thermal block, the samples, and the rear
of the device.
Instruction signs Meaning Notice
Disposal marking accord-
ing to WEEE directive
Do not dispose of the device in household
waste.
CE marking
Only for People's Republic
of China
The device contains regulated substances.
Biometra guarantees that the substances
will not leak out during the next 25 years if
used as intended.

Safety and warning instructions Biometra TOne
10
Keep ventilation slits clear Keep the ventilation slits on the underside
and at the rear of the device clear. Make
sure that there are no objects underneath
the device (e.g., paper) that may be sucked
against the ventilation slot and thus inter-
fere with ventilation.
2.3 Technical condition
The Biometra TOne has been built and certified according to safety standard EN 61010-
1. Do not modify the device in any way. Any modification of the device will lead to a loss
of warranty and the EN 61010-1 certificate and poses a potential risk.
The following has to be observed:
The operator must only operate the device in a sound and operationally safe condi-
tion. The technical condition must always comply with the legal requirements and
regulations.
Prior to every use the device must be checked for damage and sound condition.
Any changes in the device affecting its safety must be reported by the operating per-
sonnel to the operator without delay.
2.4 Requirements for the operating personnel
The Biometra TOne must only be operated by qualified specialist personnel instructed in
the use of the device. The instructions also include imparting the contents of this man-
ual.
In addition to the safety at work instructions in this operating manual the generally ap-
plicable safety and accident prevention regulations of the respective country of opera-
tion must be observed and adhered to. The operator must ascertain the latest version of
these regulations.
The operating manual must be accessible to the operating and service personnel at all
times.
2.5 Safety instructions: transport and installation
The following has to be observed:
Drain the sample block before transporting the Biometra TOne. Make sure there are
no sample tubes in the block.
Only ship the Biometra TOne in its original packaging with the transport lock in-
stalled.
Please verify that the delivery is complete on receipt of the Biometra TOne and check
for possible transport damage. In case of claims please contact Biometra GmbH.

Biometra TOne Safety and warning instructions
11
2.6 Safety instructions: during operation
2.6.1 General
The operator of the Biometra TOne must make sure before each commissioning that the
condition of the device including the safety equipment is sound.
The following has to be observed:
Free access to the power switch on the back of the enclosure has to be ensured dur-
ing operation.
The ventilation fittings at the rear of the device must be unobstructed and opera-
tional. Covered ventilation grilles or slits etc. may cause the device to break down or
may cause damage to it.
The use of oil between the samples and the sample block is not necessary to achieve
an improved heat exchange. However, if you still want to use oil, you should use
mineral oil. Do not use silicone oil.
Be careful to avoid crushing or pinching injuries when closing the device.
The Biometra TOne may cause burns. The following has to be observed:
The thermal block, the samples and the heated lid reach high temperatures. There is
a risk of burns during contact.
Ensure that the lid is securely closed before starting the program! Do not touch the
heated lid!
The rapid heating of the thermal block can cause liquids to boil explosively. Always
wear safety goggles during operation!
Do not touch hot sample tubes or plates and do not open them or boiling liquid may
escape!
Only use plates and tubes that are suitable for high temperatures (up to 100°C), fit
well into the thermal block (no shaking) and whose lids seal tightly!
2.6.2 Safety instructions: protection against explosion and fire
The Biometra TOne must not be operated in an explosive environment.
The Biometra TOne must not be operated with flammable, explosive or volatile sub-
stances.
2.6.3 Safety instructions: electrical equipment
Work on the electrical components of the Biometra TOne may only be performed by a
qualified electrical technician in accordance with the applicable electrical engineering
rules. Life-threatening electrical voltages may occur in the interior of the Biometra
TOne!
The following has to be observed:
Any work on the interior of the device may only be carried out by the customer ser-
vice of Biometra GmbH and specially authorized technicians.

Safety and warning instructions Biometra TOne
12
The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections, faulty or damaged cables, must be repaired with-
out delay.
Before opening the device it must be switched off at the power switch and the mains
plug must be disconnected from the mains outlet!
The Biometra TOne must be switched off immediately using the power switch (on
the equipment backplate) and the mains plug disconnected from the power supply if
any faults occur in the electric components.
Do not operate the device near sources of strong electromagnetic radiation (for ex-
ample, an unshielded, deliberately operated high frequency source), as these may af-
fect proper operation of the device.
2.6.4 Handling of samples, auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well as
for their safe handling. This is particularly important for radioactive, pathogenic, infec-
tious, poisonous, corrosive or otherwise dangerous substances. For details contact the
safety officer responsible for your location. When handling dangerous substances local
safety codes and guidelines must be observed. The following general notes do not re-
place the specific local regulations or the regulations in the EC safety data sheets of the
manufacturers for the auxiliary and operating materials.
The following has to be observed:
Protective goggles and rubber gloves have to be worn when handing reagents.
If only a few samples are treated, an (empty) tube of the same height must addition-
ally be placed at each corner position of the block. If the number of samples in the
block is too low, there is a risk of the tubes being damaged and sample liquid leaking
out.
For your own safety, please observe the potential infectious qualities of the exam-
ined biological material.
2.7 Safety instructions: service and repair
The Biometra TOne is usually repaired by the service department of Biometra GmbH or
its authorized and trained specialist personnel. Unauthorized repairs can damage the
device. Therefore, the operator may generally only carry out the tasks listed in section
"Maintenance and care" p. 92.
The following has to be observed:
The exterior of the device may only be cleaned with a damp, not dripping, cloth after
the device has been switched off.
Do not use alcohol (e.g., methanol or ethanol), organic solvents or abrasives to clean
the device.
Any maintenance on the device may usually only be carried out in the switched-off
condition (unless stated otherwise).

Biometra TOne Safety and warning instructions
13
Only use original spare parts, wear parts and consumables. These have been tested
and guarantee safe operation.
2.8 Behavior during emergencies
In case of danger or accidents, immediately switch off the Biometra TOne using the
main switch on the equipment backplate. Disconnect the mains plug from the power
supply!

Function and setup Biometra TOne
14
3Function and setup
The Biometra TOne thermal cycler is available in the following models:
Biometra TOne 96 with aluminum block
Biometra TOne 96G with aluminum block and gradient function
The device is controlled by a 7'' touchscreen display and an easy-to-use user interface.
PCR protocols and run log files can be saved to a connected USB stick. By using the USB
functions, PCR protocols can be exchanged easily between devices and run log files can
be stored for documentation reasons on a PC.
Fig. 1 The Biometra TOne Thermal Cycler
The Biometra TOne thermal cycler features an automatic restart. If a power failure oc-
curs during the run, the device will continue the run as soon as power is restored. In case
of long-term power failure (longer than 30 minutes), the device will keep the sample
block at 4°C (freeze step) and the user can decide to repeat the run with the same sam-
ples or to discard them.
The block with gradient function can optionally be used for the optimization of new pri-
mer pairs. The Linear Gradient Tool allows the programming of gradients with a defined
temperature difference between the rows or columns of the sample block.
This manual provides information on how to use all models of the Biometra TOne Ther-
mal Cycler most effectively. Functions that are only available for certain versions are in-
dicated accordingly, for example, "This function is only available for gradient-enabled
thermal cyclers".
3.1 High speed thermoblock
All blocks of the Biometra TOne thermal cycler are made of aluminum and offer high ramp
rates for fast protocol run times. A rubber seal on the heated lid forms an encapsulated
space around the sample block as soon as the lid is closed. The closed space serves to
improve the temperature uniformity of the sample block and avoids the formation of con-
densed water at the final PCR cooling step. Furthermore all blocks are perfectly sealed to

Biometra TOne Function and setup
15
prevent condensed water from penetrating into the Peltier elements underneath the sam-
ple block and other parts of the electronics. The sealing protects the Peltier elements and
prolongs the lifetime of the device.
3.2 High Performance Smart Lid
The Biometra TOne heated lid has been optimized to heat twice as fast as with older
models and thus helps to shorten protocol run times. Moreover the heated lid fulfills two
other important functions: It prevents the formation of condensation at the reaction tube
portion located above the block surface level and it ensures reliable contact between the
reaction tubes and the thermoblock by applying constant pressure. Thanks to the inte-
grated clutch mechanism, the pressure applied to the reaction tubes is always the same,
regardless of the plasticware height. The combination of the heated lid shape and the
reliable contact pressure ensures even temperature distribution between samples, thus
significantly improving temperature uniformity.
With one press on the front button, the heated lid gently swings open and locks in its end
position. This mechanism ensures that the lid cannot fall down and the heated lid is out
of the radius of action while the user inserts PCR tubes or plates. This prevents any risk of
injury from crushing or hot surfaces.
3.3 Display
The Biometra TOne thermal cycler features a 7" state-of-the-art color touchscreen user
interface. The touchscreen is built-in at a flat angle to ensure reflection-free viewing and
ergonomic programming. For programming PCR protocols the software incorporates Bi-
ometra's proven spreadsheet philosophy and alternatively offers graphical programming.
One touch of a button leads from the spreadsheet to the alternative graphical program-
ming mode. This makes the creation of new programs or editing existing programs fast
and easy.
3.4 User-specific quick start of programs
Many other thermal cyclers offer a so-called "List of latest programs used". In the list of
latest programs used a certain number of PCR programs is collected and offered for quick
start. The list is typically ordered only by date and the user has to search for the desired
program. In contrast the Biometra TOne software creates a user-specific list and only the
latest programs started by the currently logged-in user are offered for quick start. Even if
a lab member does not use the device for a longer time, the information on the latest
programs started by this user will not get lost.
For maximum convenience the Biometra TOne software additionally features a program
preview. Before the start of a program the protocol steps are summarized in a clearly ar-
ranged table by the program preview tool. The preview therefore provides a comprehen-
sive overview of the protocol structure without the need to access programming screens.
The program preview is also available before the quick start of programs.

Function and setup Biometra TOne
16
3.5 Easy programming
Creating new PCR programs takes a lot of time if for each single step all parameters have
to be set manually. The Biometra TOne software offers several pre-installed program
templates for different applications. The program templates provide a general protocol
structure that can be easily adapted for the current experiment.
3.6 Linear Gradient Tool
For optimizing new primer pairs in a single run the Biometra TOne can optionally be
equipped with a gradient function. Finding the best primer annealing temperature is cru-
cial for the specificity and efficiency of PCR reactions. Often the optimization of experi-
ments is conducted to a limited extent only leading to unspecific byproducts or reduced
PCR sensitivity. By using the gradient function, new primer pairs with unknown annealing
temperatures can be tested quickly and optimized in a very short time.
For maximum convenience the Biometra TOne offers the Linear Gradient Tool. For most
other devices the temperature difference from row to row or from column to column in a
gradient step varies across the sample block. The Biometra TOne Linear Gradient Tool
enables the programming of gradient steps with a defined temperature increment be-
tween the columns or rows. The Linear Gradient Tool option allows the comfortable cre-
ation of gradient steps and thus the best annealing temperature can be easily transformed
to a non-gradient protocol.
3.7 GLP compliance
The software can manage up to 30 user accounts. With each system start the Biometra
TOne performs an initial self-test and additionally the software offers an extended self-
test that can be initiated by the user. The results of the extended self-test are summarized
in a protocol and stored by the device. In addition to the self-test functions, the device
creates and stores log files for each single run. For long-term storage, the log files and
extended self-test protocols can be exported as a proprietary file format.
3.8 Open system philosophy
The Biometra TOne is an open system regarding consumables and reagents. Depending
on the sample block, the system can be used with single tubes, strips or plates. PCR plates
can be standard profile or low profile and they can be skirted, half-skirted or non-skirted.
The Biometra TOne can handle all kinds of plates and the plasticware be sealed with
domed lids, flat lids, sealing foil or other technologies. Regardless of the sealing method
used, thanks to the HPSL technology (see section 3.2) the same pressure is always applied
to the consumables for absolutely reproducible conditions.
The Biometra TOne thermal cycler is not limited to reagents from a specific vendor and
can be used with any kind of polymerase. Regardless of whether isothermal, hotstart or
non-hotstart polymerases are used, the Biometra TOne will produce reliable results. By
programming a pause step for the initial denaturation, for example, the system can be

Biometra TOne Function and setup
17
used for manual hotstart applications. After inserting the samples into the heating block,
the PCR protocol can be started just by pressing "continue".
3.9 Order numbers
Instruments incl. block and base unit
Model Block type Gradient range Order number
TOne 96 Aluminum - 846-x-070-311
TOne 96G Aluminum 20°C 846-x-070-301
Abbreviations:
G = gradient
x = 2 for 230 V, 4 for 115 V, 5 for 100 V

Installation Biometra TOne
18
4Installation
4.1 Scope of delivery
1. Thermal cycler
2. Mains cable
3. Operating instructions
4. Quick reference guide
Please keep the original packaging in case the device needs to be returned. The shipping
box for the Biometra TOne thermal cycler contains a specially developed foam system
for the transport of high quality electronic devices.
4.2 Unpacking and inspection
Unpack and carefully check the device. Immediately report any damage to Biometra
GmbH. Do not attempt to operate the device if there is any visible damage.
Keep the original packaging for possible return. The Biometra TOne must always be
transported in its original packaging. If the packaging is no longer available, please con-
tact Biometra GmbH.
4.3 Setting the operating voltage
WARNING
Danger of electric shock! Before commissioning the device, make sure that the voltage
selector setting on the underside of the device correspon
ds to the mains voltage in your
laboratory.
The Biometra TOne can be operated at 100, 115 or 230 volts.
Set the operating voltage by turning the dial on the underside of the Biometra TOne
to the desired voltage. You can do so with a coin.
Fig. 2 Voltage selector switch on the underside of the device

Biometra TOne Installation
19
4.4 Commissioning
4.4.1 General setup
WARNING
Danger of electric shock!
Prior to commissioning, please make sure that the operating
voltage set on the device is
the same as the mains voltage (see section "Setting the oper-
ating voltage
" p. 18)
NOTICE
Do not cover the ventilation slits!
Ensure that the ventilation slits are clear. Insufficient
ventilation may cause the device to overheat.

Make sure that there are no objects underneath the device (e.g., paper) that may be
sucked against the ventilation slot and thus interfere with ventilation.

Place the device at a minimum distance of 10 cm between the rear of the device and
the wall or other objects.

In case of several cyclers side by side running simultaneously we would recommend
to keep a distance of at least 10 cm between every thermal cycler.
Place the Biometra TOne on a solid dry surface.
If the Biometra TOne has been moved, allow the device to adjust to room tempera-
ture before switching it on for the first time (1 to 6 hours).
Connect the Biometra TOne to a grounded mains outlet using the mains cable sup-
plied.
Make sure that the mains switch and the mains cable are easily accessible. This is
important if the device needs to be disconnected from the mains voltage.
The display contrast can be adjusted according to the respective lighting conditions
(see section "Display brightness" p. 66).
4.4.2 Installation when used in a network
When using the thermal cycler together with the Biometra TSuite thermal cycler man-
agement computer software, please follow these instructions for connection in a net-
work:
The thermal cycler can be connected to a computer via the company network. The
thermal cycler is usually configured for a network connection with a DHCP server in
the delivery state (see section "Network" p. 65).
This requires an Ethernet cable (minimum requirements: Cat 5e performance class,
STP cable structure, max. 30 m length).
You can connect the thermal cycler and the computer to the DHCP server via the
Ethernet cable and a router (e.g. such a device https://www.tp-link.com/us/home-
networking/wifi-router/tl-wr841n/), alternatively you can connect the computer
and the thermal cycler to the company network if this is allowed by your company's
IT policies.

Installation Biometra TOne
20
For an alternative direct connection of the thermal cycler, the thermal cycler must be
changed to a network connection with a fixed IP (e.g. IP 192.168.0.30, subnet mask
255.255.255.0) (see section "Network" p. 65).
The network card must also be changed to a fixed IP(V4) via the network adapter
settings under Windows (e.g. IP 192.168.0.10, subnet mask 255.255.255.0).
The PC network card should comply with the IEEE 803.2ab_1999 autocrossover
standard, otherwise a crossed network line is required. Newer network cards usually
comply with this standard.
An Ethernet cable is available as an optional accessory.