OPTO-EDU A15.1018 User manual

A15.10 18
Polarizing Microscope, Transmit Light
Instruction Manual
To ensure the safety and obtain satisfactory performance, please study this instruction manual thoroughly be
fore start to use the instrument.

1
CONTENTS
CONTENTS ………………………………………………………… 1
USER NOTIECE …………………………………………………… 2
1、NOMENCLATURE ………………………………………………… 4
2、ASSEMBLY……………………………………………………………… 5
2-1.Assembly Diagram …………………………………………………… 5
2-2.Detailed Assembly Procedure……………………………………… 6
3、USING THE CONTROLS…………………………………………… 9
4、POLARIZED LIGHT OBSERVATION ……………………… 11
5、SPECIFICATIONS………………………………………………… 13

2
USER NOTICE
Ⅰ.Safety Precautions
1. Carefully open the box, avoid the accessories, like lens, dropping to ground and
being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty
and easy shaking environment. Make sure the stage is smooth, horizontal and
firm enough.
3. When moving the instrument, please use two hands to grip with the two sides of
the microscope body.
4. When running, the lamp house and nearby parts will be very hot. Please ensure
there is enough cooling room for them.
5. Make sure the instrument is earthed, to avoid lighting strike.
6. For safety, be sure the main switch is in “O”(off) state before replace the halogen
lamp or the fuse, then cut off the power, and do the operation after the lamp bulb
and the lamp house completely cool (designated bulb:. 24V/100W halogen
lamp)
7. Use the factory supplied power cord, please.
II. Maintenance
1. All the lenses have been well checked and adjusted. It is forbidden to
disassemble them yourself.
2. The nosepiece and coarse/fine focus unit have a compact and precise frame, so
please don’t disassemble them as possible as you can.
3. Keep the instrument clean, wipe dust regularly, and be attention to avoid
contaminating the optical elements especially.
4. The contaminations on the prism, as finger mark and oil, could be gently wiped with
a piece of soft cloth or tissue paper, gauze which has been immersed in pure
alcohol or xylene. (Note that the alcohol and the xylene are all burned easily, do
not let them near the fire, and use them in a drafty room as possible as you
can.)
5. Don’t use organic solvent to wipe the non-optical elements, when you need to
clean, use the soft detergent, please.
6. When using, if the microscope is splash by liquid, cut off the power at once, and

3
wipe up the moisture.
7. Do not disassemble any parts of the microscope. That will affect the function or
decline the performance of the microscope.
8. Place the instrument in a cool, dry position. After using the microscope,
remember to cover it with dust helmet. Do wait for the lamp house cooling
completely before cover.

4
1. NOMENCLATURE
Video port
Eyepiece
Objective
Revolving round stage
Lamp housing
Trinocular viewing head
Reflected illumination system
Bertrand lens unit
Main body

5
2.Assembly
2-1 Assembly Diagram
The diagram below shows how to assemble the various components. The numbers
indicate the order of assembly.
 When assembling the components, make sure that all parts are free of dust and
dirt, and avoid scratching any parts or touching glass surfaces.
 Keeping the hexagon- spanner well, when change the spare parts, you will use
it.
①
②
③
④
⑦
⑤
⑥
②
⑧
⑨
⑩

6
Fig.2
③
Fig.1
Fig.3
2-2 Detailed Assembly Procedure
2-2-1 Installing the Polarizing Stage
Support Device
1. Before installing the device, be sure to
adjust the coarse focus knob①. Make
the guide board② (see figure 1) down
to the lowest position, so you can
install the mechanical stage support
device easily.
2. Hold on the mechanical stage support
device (figure 2), place it from the top
of the guide board (figure 1), and let
the device (figure2) falling free until it
reach the limit position. Use the
hexagon wrench screw down the
locking block ③to make the stage
support device and the guide board
fixed together.
2-2-2Mouting Revolving Round
Stage
1. Loosen the centering knobs④。
2. Position the stage with the venire scale
⑤in front, and carefully lower the stage
onto the round dovetail on the stage
bracket⑥ with the positioning pin on
the stage aligned with the groove on the
front of the stage bracket, then loosely
tighten the centering knobs④. Turn the
stage to check it is mounted properly,
finally tighten the centering knob.
Mounting Stage Clips
Insert the stage clips securely into any two
holes⑦ on the stage top surface.
 Mounting Polarizing Ruler
Insert the positioning pins on the underside
of the ruler into the positioning holes⑧ on
the stage surface, using the Allen
screwdriver provided with the microscope
frame, tighten the clamping
screw.
②
①
④
⑤
⑥
⑧

7
Fig.5
Fig.7
Fig.6
2-2-3 Installing the Objective
1. Adjusting the coarse focus knob until the support
device of the mechanical stage reach its low limit
position.
2. Insert the 10X objective into the primary hole
(which without centering holes on its sides) on the
nosepiece, then push the nosepiece clockwise and
install other objectives by the sequence of low to
high magnification ( figure 5).
 Installing objective in this way will make the
change of magnification to be easier while in
using.
 Clean the objective regularly as it is very
sensitive to dust.
 When operating, use 10 ×magnification
objective to search specimen and focus firstly,
then replace with higher magnification
objective if necessary.
 When replacing the objective, slowly turning
the nosepiece until you hear “clicked”, that
means the objective enter the required
position--the light path center.
2-2-4Mounting reflected illumination system
(Fig.6)
1. Mount the Illumination System① into the round
dovetail on the head of the microscope body
properly and use the Allen screw driver to tighten
the set screw② to fix it.
2-2-5 Mounting Bertrand lens unit (Fig.6)
1. Mount the Bertrand lens unit③ into the round
dovetail on the reflection Illumination System①t
properly and use the Allen screw driver to tighten
the set screw④ to fix it.
2-2-6 Mounting trinocular viewing head (Fig.7)
1. Mount the trinocular viewing head⑤ onto the
illumination system properly and use the Allen
screw driver to tighten the bolt⑥）to fix it.
⑦
①
②
③
④
⑤
⑥
⑦ ⑧

8
Fig.9
Fig.8
2-2-7 Installing the Video Port (Fig.7)
Insert the video port ⑦into the trinocular viewing head, then screw down the bolt⑧ to
fix it.
2-2-8 Installing the Eyepiece (Fig.7)
Insert the eyepiece into the eyepiece tube until they are against each other.
2-2-9 Installing the lamp house
1. Push the lamp house into the illumination kits (Fig. 8)
gently until they are against each other, turn to a proper
position and then screw down the bolt①to fix it.
2. Insert the power plug② into the power jack of the
microscope③/④, and screw down the bolt to fix it.
For transmitted light illumination, the power jack③ is
used. For reflected light illumination, the power jack④ is
used.
2-2-10 Mounting Polarizer and Analyzer
1. Insert the polarizer⑤ into its slot with the silkscreen
surface toward the front and make it in the light path
center.
2. push the analyzer⑥ into its slot.
NOTE: the polarizer and analyzer are engaged when
pushed in to the second click-stop position. To
disengaged the polarizer and analyzer, and to place the
empty hole in the light path, pull the analyzer out to the
first click-stop.
2-2-11 Mounting Test Plate Compensator
1. Insert the optical compensator⑦ into the test plate
adapter, and securely tighten the clamping knob⑧,as
shown in fig.9.
①
② ③
④
⑤
⑥
⑦
⑧

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3. USING THE CONTROLS
Polarizer
检偏器
Polarizer clamping
Compensator
Vernier scale
平台支紧螺钉
Polarizing
调节旋钮
起偏器转环
勃氏镜转盘
Analyzer
Bertrand lens unit
Bertrand lens
focusing plate
Compensator
clamping knob
Polarizer clamping knob
Polarizer rotation ring
Polarizing ruler
adjustment knob
Stage rotation
clamping knob

10
Fig.10
Fig.11
Fig.12
Using controls
3-2-1 Stage Rotation
When the stage rotation clamping knob
① is loosened, the stage can be rotated
horizontally through 360°.
3-2-2 Using 45° Click Stop
Lever
When the 45° click stop lever② is
moved toward the observer, and the
stage is moved from this position to
the first click-stop position, the specimen
is moved 45° to its diagonal position.
To release the 45° click stop function,
push back the lever.
3-2-3 Using the Bertrand Lens
By revolving the Bertrand lens dial③,the
Bertrand lens can be selected. At the“O”
position, the lens is removed from the
light path. At the “B” position, the lens
is engaged.
3-2-4 Focusing the Bertrand Lens
During conoscopic observation, to focus
the conoscopic image, turn the Bertrand
lens focusing ring ④ slightly until a
clear interference image is obtained in
the eyepiece.
①
②
③
④

11
Fig.13
Fig.15
Fig.14
4. Polarized Light Observation
4-1 Adjustments before observation
4-1-1 Focus
Use the 10×objective to focus. To avoid the
objective touch with the specimen, you should
raise the mechanical stage and let the specimen
close to the objective, then slowly separating
them to focus. The operator can converse turn
the coarse focus knob to get the specimen
down ,and search images in the 10×ocular
simultaneously, then use the fine knob to focus.
At this moment, you can replace other
magnification objectives safely, and focus
without the risk of destroying the specimen.
The tension of the coarse focusing knob has been
adjusted before leaving factory. If it is too loose,
for example, the stage decline slightly due to
self-weight, the tension adjustment collar can be
adjusted.
4-1-2 Adjusting the optical axis (Fig.13)
The revolving nosepiece is centerable. Before
leaving the factory, the optical axis of the
objective and the center of stage has been
adjusted coaxial, please do not adjust them
unless necessary.
★ Make sure the 10X objective is inserted into the
primary hole on the centerable revolving
nosepiece.
Perform the following adjustments if the optical
axial and the center of stage is not coaxial.
1. Focus on the specimen by 10x objective and look
for an easily recognizable detail① in the field.
Move this detail in the center of the eyepiece cross
lines.
2. When the stage is rotated, the detail moves in circle
②. Manipulate the two stage centering knobs③to
coincide the imaginary center of the circle
circumscribed by the detail with the intersection
of the eyepiece cross lines.
②
①
③
④

12
Fig. 17
Fig.16
4-2 Orthoscopic Observation
Orthoscopic observation is available for
4X～ 100X objectives.
(1)Revolve the Bertrand lens dial① to
“O” position to remove the Bertrand
lens from optical path.
(2)Swing out the top lens of the
condenser.
(3) For reflected illumination system, the
polarizer is fixed and the analyzer can be
360 °rotatable. Rotate the analyzer ②
until complete extinction is obtained.
（4）For transmitted illumination system,
the polarizer can be 360°rotatable, turn it
until complete extinction is obtained.
（5）place the specimen for orthoscopic
observation.
（6）insert test plates for further
observation test and study.
4-3 Conoscopic Observation
Use 20X to 100X objectives。
（1）Engage the polarizer and analyzer for
extinction position
（2）Swing the condenser top lens into the
light path.
（3）revolve the Bertrand lens dial①to“B”
position to engae the lens into the light
path.
（4）open the aperture iris diaphragm④ to
largest size.
（5）revolve the focusing dial⑤ of the
Bertrand lens to focus on the conoscopic
image.
①
③
②
④
⑤
3. Moving the specimen only, move a new specimen detail into the cross line center.
4. Repeat steps 2 and 3 several times until the center of the stage rotation is in the center of the
cross lines, i.e., when rotating the stage, the specimen stays in the center of the cross lines.
This concludes centering of the optical axis for the 10X objective, which will act as the reference
objective. Now, center the other objectives with the centerable nosepiece by engaging the
objectives into the light path one after another.( each objective has 2 centering screw④on the
nosepiece.

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5．Technical Specifications
1. Main specifications
1.system：Infinite system
2.polarized objective（plan）
Magnification Numerical
aperture
（NA）
Thickness of
cover
slip(mm)
Working
distance
（mm）
Work
type
illumination
5× 0.12 - 15.4 dry T/R
10× 0.25 - 10 dry T/R
20× 0.40 - 5.1 dry T/R
40× 0.65 0.17 0.35 dry For transmitted light
100× 1.25 0.17 0.13 oil For transmitted light
50× 0.75 0 dry For reflected light
100× 0.8 0 dry For reflected light
3. Eyepiece with reticule
Category Magnification Field of view（mm）
Plan eyepiece 10× Φ22
Crisscross eyepiece 10× Φ22
Eyepiece with scale of
crosshair
10× Φ22
eyepiece with gridding 10× Φ22
4. Total magnification
eyepiece 10× 10× 10× 10× 10× 10×
objective 5× 10× 20× 40× 50× 100×
Total
magnification
50× 100× 200× 400× 500× 1000×
5．Rotatable stage：Diameterф170mm，360°scale。Minimum reading 6’by means of
vernier scale
6. Coaxial Coarse and Fine Focusing System,
Sensitivity and Graduation of Fine Focus: 0.001mm
Focusing movement range：32mm
7．Polarizer: can be swung out
8．Analyzer:0°-360°rotatable
9．Bertrand lens: can be removed from optical path
10. Test plate compensator：1/4λ test plate,λtest plate as well as quartz wedge test
plate, inserted at 45 degree
11．Condenser: NA=0.9/0.25 top lens swing-out type
12．Illumination system: Koehler Illumination, halogen lamp 24V100W for reflected
and transmition light, brightness adjustable
Note:If the periphery of the conoscopic image is dark, move the condenser vertically to find
the position where the periphery is brightest.

14
2. Configuration Table
item Specifications TRF RF M
Main Body
Main frame ● ● ●
Rotatable stage ● ● ●
polarized ruler ○ ○ ○
Condenser bracket ● ● ●
Viewing
system Trinocular tube ● ● ●
Polarizing
attachment
polarizer ● ● ●
Analyzer
0°-360°rotatable ● ● ●
Bertrand lens:
removable
● ● ●
nosepiece Centerable quintuple ● ● ●
Test plate
compensato
r
Λtest plate（first-order
red）
● ● ●
1/4λtest plate ● ● ●
Quartz wedge test
plate
● ● ●
Empty test plate ● ● ●
filter ND25、ND6 ○ ○ ○
fitler Blue,yellow,
green,frosted
● ● ●
illumination
24V100W
halogen lamp
● ● ●
Spare lamp ● ● ●
eyepiece
10×plan eyepiece ●/2 ●/2 ●/2
10× Crisscross
eyepiece
● ● ●
10× eyepiece with
scale of crosshair
● ● ●
10× mesh eyepiece ● ● ●
Strain-free
objective
Plan 5× ● ● ●
Plan 10× ● ● ●
Plan 20× ● ● ●
Plan 40× ● / ●
Plan 100×（oil） ● / ●
Plan 50× ● ● /
Plan 100× ● ● /
condenser
Swing-out type
condenser
NA=0.9/0.25
● ● ●

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