Fisher Scientific FB-705 User manual

Rev. 6-12
1
Sonic Dismembrator
ULTRASONIC PROCESSOR
Part No. FB-705
OPERATION MANUAL

Rev. 6-12
2

Rev. 6-12
3
Table of Contents
Section Page No.
1. arranty 4
2. arnings 5-6
3. Specifications 7-8
4. Principles of Operation 9-10
5. Description of Components / Functions of Controls 11-13
6. Preparation for Use 14
7. Operating Instructions(Getting Started) 15-24
8. Accessories 25-26
9. Maintenance 26-28
10. Troubleshooting 29
11. Service / Return of Equipment 30
12. Service Safety Certification form 31
13. Addendum 32-34

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1. arranty
Your Ultrasonic Processor is warrante an backe by the manufacturer for a perio of two
years from the ate of shipment against efects in material an workmanship un er normal use
as escribe in this instruction manual. During the warranty perio , the manufacturer will, at its
option, as the exclusive reme y, either repair or replace without charge for material an labor,
the part(s) which prove to be efective, provi e the unit is returne to us properly packe with
all transportation charges prepai .
Ultrasonic probes are guarantee against efects for a perio of one year from ate of
shipment. A efective probe will be replace once without charge, if failure occurs within the
warranty perio . Wear resulting from cavitation erosion is a normal consequence of ultrasonic
processing, an is not covere by this warranty.
The manufacturer neither assumes nor authorizes any person to assume for it any other
obligations or liability in connection with the sale of its pro ucts. The manufacturer hereby
isclaims any warranty of either merchantability or fitness for a particular purpose. No person or
company is authorize to change, mo ify, or amen the terms of this warranty in any manner or
fashion whatsoever. Un er no circumstances shall the manufacturer be liable to the purchaser
or any other person for any inci ental or consequential amages or loss of goo will, pro uction,
or profit resulting from any malfunction or failure of its pro uct.
This warranty oes not apply to equipment that has been subject to unauthorize repair,
misuse, abuse, negligence or acci ent. Equipment which, shows evi ence of having been use
in violation of operating instructions, or which has ha the serial number altere or remove , will
be ineligible for service un er this warranty.
All probes are manufacture to exacting specifications an are tune to vibrate at a specific
frequency. Using an out-of-tune probe will cause amage to the equipment an may result in
warranty nullification. The manufacturer assumes no responsibility for probes fabricate by
another party or for consequential amages resulting from their usage.
The aforementione provisions o not exten the original warranty perio of any pro uct that
has either been repaire or replace by the manufacturer.

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2. arnings
Please read the manual in its entirety. Necessary instruction and guidance are provided to help
ensure the successful operation of this device.
Your new Ultrasonic Liqui Processor has been esigne , built an teste to assure maximum operator
safety. However, no esign can completely protect against improper use that may lea to bo ily injury
an /or property amage. For total safety an equipment protection, rea the instruction manual carefully
before attempting to operate this equipment. Observe the following ARNINGS:
 High voltage is present in the generator (power supply), converter an high frequency cable. There are no user-
serviceable parts insi e any of these evices. Do NOT attempt to remove the generator cover or converter case.
 Do NOT touch any open cable connections on the unit while the power is turne ON.
 Do NOT operate generator with converter isconnecte from high voltage cable. High voltage is present in the
cable an may pose a shock hazar .
 Do NOT attempt to isconnect the converter high voltage cable while the unit is running.
 The generator must be properly groun e with a 3-prong plug. Test electrical outlet for proper groun ing before
plugging in unit.
 Install the ultrasonic processor in an area free from excessive ust, irt, explosive or corrosive fumes an
protecte from extremes in temperature an humi ity. Do not place the Generator within a Fume Hoo .
 Hearing protection is highly recommen e . It is recommen e that a soun abating enclosure or ear protection
be use when operating the Ultrasonic Processor
 NEVER immerse the converter in liqui s of any kin , or let con ense moisture or liqui rip into the converter.
 NEVER grasp an activate horn or touch the tip of a vibrating probe. It can cause severe burns an tissue
amage.
 NEVER allow a microtip to vibrate in air for more than 10 secon s.
 NEVER hol or clamp the converter by the front river or by the horn itself. This can cause permanent amage
to the system. Support the converter by only clamping aroun the converter housing (upper portion).
 If nee e air cool the convertor with ry compresse air.
 Do NOT allow the tip of a vibrating horn or probe to touch the counter top or any other har surface. It coul
amage the probe, overloa the generator, or amage the surface.
 Avoi touching the bottom or si es of a glass or plastic container with an activate probe. It coul crack or
shatter the glass or melt the plastic.
 Turn OFF the power switch, unplug the generator an isconnect the power cor from the back of the generator
before attempting to replace the fuses.
 Inspect high frequency cable for cracks in the protective outer jacket.
 Do not operate unit with a amage cable. Doing so may cause serious injury.
 In case of AC power loss, wait 3 minutes minimum before reapplying power.
 Do not turn off AC mains power while running a horn. Stop sonication through the touch screen prior to removing

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AC power.
Symbols
Caution, Risk of electric shock, Hazar ous voltage.
Caution, Risk of anger. Refer to User Manual.

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3. Specifications
Generator
Input Voltage 100 VAC – 120 VAC @ 50/60 Hz 220 VAC – 240 VAC @ 50/60 Hz
Rate Current
12 Amps max. 6 Amps max.
Fuse Rating
15 Amps* 8 Amps*
Weight
16 lbs. (7.3 Kg)
Dimensions 8"W x 15"L x 9"H
203 mm x 381 mm x 229 mm
Output Voltage 1000 V rms (max.)
Output Frequency 20 KHz
Converter
Weight 2 lbs. (900 g)
Dimensions 7.25" L x 2.5" Dia.
(183 mm x 63.5 mm)
Materials Aluminum Alloy
Standard ½ ” Horn
Weight 0.75 lbs. (340 g)
Dimensions 5.375" L x .5" Dia.
(136 mm x 13 mm)
Materials Titanium Alloy

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Environmental
Pollution Degree 2
Installation Category II
Operating Limits
Shipping/Storage
Temperature: 41 - 104ºF (5 - 40ºC)
Relative Humi ity 10 - 95% (Non Con ensing)
Altitu e: 6,651 ft. (2000 m)
Temperature: 35 -120
o
F (2 - 49
o
C)
Relative Humi ity 10 - 95% (Non Con ensing)
Ambient Pressure Extremes: 40,000 ft. (12,192 m)
Restriction of
Hazar ous
Substances (ROHS)
Relative humi ity Maximum relative humi ity 80% for temperatures up to 31ºC ecreasing
linearly to 50% relative humi ity to 40ºC
Other For in oor use only
*Only use IEC approve Fast acting fuses, Cooper Bussman series S500.
The Power Cord supplied with the ultrasonic processor must be used. If the 220V plug is not
configure to match the wall receptacle, a properly groun e universal AC socket a apter must be
a e .
Important: Universal a apters o not convert voltage or frequency. The manufacturer is not responsible
for amage cause by the use of an improper power cor or a apter. Transformers are not
recommen e .
EEE Statement
This pro uct contains electrical or electronic materials. The presence of these
materials may, if not ispose of properly, have potential a verse effects on the
environment an human health. Presence of this label on the pro uct means it shoul
not be ispose of as unsorte waste an must be collecte separately. As a
consumer, you are responsible for ensuring that this pro uct is ispose of properly.
To fin out how to properly ispose of this pro uct contact Customer Service.

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4. Principles of Operation
The ultrasonic electronic generator transforms AC line power to a 20 KHz signal that rives a
piezoelectric converter/trans ucer. This electrical signal is converte by the trans ucer to a mechanical
vibration ue to the characteristics of the internal piezoelectric crystals.
The vibration is amplifie an transmitte own the length of the horn/probe where the tip longitu inally
expan s an contracts. The istance the tip travels is epen ent on the amplitu e selecte by the user
through the touch screen pa . As you increase the amplitu e setting the sonication intensity will increase
within your sample.
In liqui , the rapi vibration of the tip causes cavitation, the formation an violent collapse of microscopic
bubbles. The collapse of thousan s of cavitation bubbles releases tremen ous energy in the cavitation
fiel . The erosion an shock effect of the collapse of the cavitation bubble is the primary mechanism of
flui processing.
The probe tip iameter ictates the amount of sample that can be effectively processe . Smaller tip
iameters (Microtip probes) eliver high intensity sonication but the energy is focuse within a small,
concentrate area. Larger tip iameters can process larger volumes, but offer lower intensity.
The choices of a generator an horns/probes are matche to the volume, viscosity an other parameters
of the particular application. Horns are available for both irect an in irect sonication. The Accessories
section has more information on this subject.
Please consult with a product specialist for assistance with selecting a probe for your application.
Relationship of Amplitude and attage
Sonication power is measure in watts. Amplitu e is a measurement of the excursion of the tip of the
probe (probe is also known as a horn).
Some ultrasonic processors have a wattage isplay. During operation, the wattage isplaye is the
energy require to rive the ra iating face of a probe, at that specific amplitu e setting against a specific
loa , at that particular moment. For example, the unit experiences a higher loa when processing
viscous samples then when compare to aqueous samples.
The spee /cruise control on an automobile, can, to a certain extent, be compare to an Ultrasonic
Processor. The spee /cruise control is esigne to ensure that the vehicle maintains a constant rate of
travel. As the terrain elevations change, so o the power requirements. The cruise control senses these
requirements, an automatically a justs the amount of power elivere by the engine in or er to
compensate for these ever changing con itions. The greater the terrain rate of incline an greater the
resistance to the movement of the vehicle, the greater the amount of power that will be elivere by the
engine to overcome that resistance an maintain a constant spee .
The ultrasonic processor was esigne to eliver constant amplitu e, to your liqui sample, regar less of
these changes in loa (much like the vehicle’s cruise control escribe above). As a liqui is processe ,
the loa on the probe will vary ue to changes in the liqui sample (i.e. viscosity, concentration,
temperature, etc.). As the resistance to the movement of the probe increases (increase loa on the
probe), a itional power will be elivere by the power supply to ensure that the excursion at the probe
tip remains constant. The isplaye wattage rea ings will vary as the loa changes, however the
amplitu e will remain the same.
The resistance to the movement of the probe etermines how much power will be elivere to maintain
amplitu e. For example, a ½” probe at 100% amplitu e will require approximately 5 watts to operate in
air. The amplitu e of this probe is approximately 120um. Insert the probe in water an the wattage
rea ing will increase to approximately 90 watts. The wattage require to operate the probe will increase

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as the loa increases but the amplitu e remains the same.
The AMPLITUDE control allows the ultrasonic vibrations at the probe tip to be set to any esire level.
Although the egree of cavitation/ultrasonic energy require to process the sample can rea ily be
etermine by visual observation, the amount of power require cannot be pre etermine . A sensing
network continuously monitors the output requirements, an automatically a justs the power to maintain
the amplitu e at the preselecte level. The greater the resistance to the movement of the probe ue to
higher viscosity, eeper immersion of the probe into the sample, larger probe iameter or higher
pressure, the greater the amount of power that will be elivere to the probe. Setting the AMPLITUDE
control to its maximum will not cause the maximum power rating of the unit to be elivere to the sample.
The maximum power (705 watts) that the Ultrasonic Processor is capable of elivering will only be
elivere when the resistance to the movement of the probe is high enough to raw maximum wattage.
It is the intensity of cavitation that measures the effectiveness of the sonication, not the total power
applie to the system. Intensity is irectly relate to the amplitu e of the ra iating face of the tip or horn.
It is amplitu e that must be provi e , maintaine , an monitore . The unit provi es controlle amplitu e
un er varying loa con itions in or er to give repro ucible results.

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5. Description of Components / Functions of Controls
The Model # FB-705 includes a standard /2” diameter probe (#FB4220).
FB-705 Front Panel
FB-705 Rear Panel
FUNCTIONS OF KEYS, CONTROLS, INDICATORS, AND CONNECTORS
Generator
Converter
½” Horn
with
replaceable tip
Converter
Cable
Power Cord
Connector
Converter
Cable
Connector
On/Off
Switch
Temperature Probe
Connector
Footswitch
Jack
Cooling Fan
Fuses

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FRONT PANEL
Touch screen isplay
Displays prompts an control parameters inclu ing:
• Amplitu e selecte
• Output power elivere to the probe in watts
• Selecte uration of processing
• Actual processing time
• Elapse time
• Set an rea temperature
• Pulse on/off uration
• Accumulate amount of energy in Joules elivere to the probe
0 – 9 key Input igits for programming screens
EXIT
key Moves to previous screen
ENT
key Completes parameter entry on programming screens
CLR
key Deletes selecte parameter entry
RUN
key To procee to program run screen
SAVE
key
Stores a program to the selecte memory number. Up to 10
programs (0-9) can be store .
START/STOP
key Starts or stops the ultrasonics.
PAUSE
key
Suspen s operation, without clearing program/run totals. Press
pause again to resume program/run an continue accumulating run
ata.
▲ ▼
key
Use to set the amplitu e of vibration at the probe tip. Also use to
increase or ecrease the amplitu e in small increments while the unit
is runnng.
For explanations of each screen and button on the ultrasonic processor and complete
programming instructions please see Section 7 of this manual.

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REAR PANEL
On / Off Switch Turns the power supply on an off.
Footswitch Connector Connects to the footswitch cable.
Temperature Probe
Connector Connects to the Temperature monitoring probe or thermocouple
Converter Cable
Connector (Output) Connects to the converter.
Power Supply Connector Connects to the electrical line cor an encases the fuse(s).

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6. Preparation for Use
INSPECTION
Prior to installing the ultrasonic processor, perform a visual inspection to etect any evi ence of
amage, which might have occurre uring shipment. Before isposing of any packaging
material, check it carefully for small items.
The ultrasonic processor was carefully packe an thoroughly inspecte before leaving our
factory. The carrier, upon acceptance of the shipment, assume responsibility for its safe
elivery. Claims for loss or amage sustaine in transit must be submitte to the carrier.
If amage has occurre , contact your carrier within 48 hours of the elivery ate. DO NOT
OPERATE DAMAGED EQUIPMENT. Retain all packing materials for future shipment.
ELECTRICAL REQUIREMENTS
The ultrasonic processor requires a fuse , single phase 3-terminal groun ing type
electrical outlet. For power requirements, check the label on the back of the unit.
INSTALLING THE ULTRASONIC PROCESSOR
The ultrasonic processor shoul be installe in an area that is free from excessive ust, irt,
explosive an corrosive fumes, an extremes of temperature an humi ity. If processing
flammable liqui s, use an approve fume hoo an o not place the power supply in the fume
hoo .
When positioning the unit, be sure to leave a equate space behin the unit so that all
connections can be easily isconnecte .
WARNING
For your personal safety, o not, un er any
circumstances, efeat the groun ing feature of the
power cor by removing the groun ing prong.

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7. Operating Instructions (Getting Started)
CAUTION
• Do not operate the power supply unless it is connecte to the converter.
• Never allow liqui to spill into the converter.
• Do not allow a Microtip to vibrate in air.
• Do not allow the vibrating Microtip to contact anything but the sample.
• Never place a washer between the converter, probe or horn.
• Never apply grease to the mating surfaces or threa s of the converter, probe or Microtip.
• Shoul it become necessary to remove a probe, use the wrenches supplie . Never
attempt to remove the probe by twisting the converter housing or hol ing it in a vice, as
this may amage the electrical connections within the housing.
CAUTION
LO SURFACE TENSION LIQUIDS – ORGANIC SOLVENTS
The probes (soli or with a replaceable tip) are tune elements that resonate at a specific frequency. If
the replaceable tip is remove or isolate from the rest of the probe, the element will no longer resonate
at that frequency, an the power supply will fail. Unlike aqueous (water base ) solutions which rarely
cause problems, solvents an low surface tension liqui s are problematic. These liqui s penetrate the
probe/replaceable tip interface, an force the particulates into the threa e section isolating the tip from
the probe. When processing low surface tension liquids, ALWAYS use a solid pro e.
Set-up:
1. Connect the power cor into the receptacle on the rear of the ultrasonic processor.
2. Make sure the unit is switche off. Plug the electrical line cor into the electrical outlet.
3. If the optional foot switch is use , insert the plug into the jack locate on the rear panel.
4. For best results it is critical to use the appropriate size an type of accessory to process
your sample. If you are not sure that you have the proper horn for your sample volume
please refer to the Accessories section of this manual or call the manufacturer for
assistance.
5. Horns/Probes must be properly tightened. Depen ing on the accessories purchase ,
often the horn an the flat tip are attache to the converter at the factory. Check the
tightness of the horn and flat tip by using the wrench set. Please refer to images in the
Maintenance section of this manual. A loose horn or tip may cause amage to the
generator circuitry or parts of the converter an horn. A loose horn may also show a
fluctuation in wattage rea ings. Always use the wrenches supplied with the unit.
6. If you will be using a Microtip or exten er, remove the flat tip on the en of the replaceable
tip probe, then attache the Microtip or exten er in its place.
7. Horns an probe tips wear after normal usage. Using a severely worn probe tip can
amage internal generator components.
8. If using a laboratory stan , mount the convertor /probe assembly using a clamp. Be sure to

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secure the clamp to the upper section of the convertor housing only. Never secure the
clamp to any other portion of the convertor/probe assembly. If you are using an acoustic
enclosure mount the convertor properly in the convertor collar.
9. Connect the converter cable to the power supply an then to the top of the convertor. Push
the connectors in an turn the chrome rings clockwise ¼ turn to secure the connectors.
10. If application or proce ure instructs that the unit be run for a perio of time that will allow the
probe/convertor assembly to be warm to the touch, it is recommen e that the convertor be
air coole with ry compresse air. Never run the convertor and horn assem ly if it
ecomes hot. Connect either one of the air fittings on top of the converter to a source
of dry compressed air. This will cool the convertor so that it can function normally
under load for an extended duration without ecoming too hot.
Operation:
Your new ultrasonic processor has been esigne with a color LCD user interface with touch
screen capabilities. All program an run functions are controlle through the touch screen
panel.
This is the first screen that appears after Ultrasonic processor is switche On.
1. Answer the Microtip Question, Yes or No.
When using a Microtip, the YES button must be selecte .
*See the Accessories (section 8, pg 25) for an image of a microtip. If you are not
familiar with the microtips and need assistance, please call your representative for help.
arning: Improper selection may result in tip amage or poor sonication. Damage cause by
not following this step is not covere un er the warranty.
After answering the Microtip question, the following screen appears:

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This screen allows the user to select Manual mo e, Program menu or Options screen.
2. Select the Mo e of Operation or access Options screen.
a. Manual Mo e: Selecting Manual Run allows the user to set the unit output level
manually (setting 1 – 100%). Starting an stopping the ultrasoun output is also
manually performe .
b. Program Mo e: Allows the user to create a program with specific On / Off times
an output setting. The ability to save up to 10 programs an sequence several
in ivi ual programs together can also be performe in this mo e.
c. Options: Allows the user to change isplay contrast, an select either ºC or ºF if
temperature monitoring is enable . Footswitch an temperature probes are
optional items an are not shippe unless specifically or ere with the unit.
To Manual Mode
To Option Screen
To Program Menu
To Previous
Screen

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The following screen isplays the Manual Mode.
A. Microtip Mo e – In icates that the unit is set for use with microtips only. This mo e shoul
not be use with stan ar probes or horns (1/2” or larger). See page 15 for information on
selecting YES or NO at the “Are you using a Microtip screen”.
B. Amplitu e (intensity) setting - Output amplitu e may be set between 1-100%.
C. Power isplaye in Watts.
D. Energy isplaye in Joules.
E. Elapse Time – Total time of active sonication.
F. Temperature probe measurement (note: if “OPN” appears, it in icates temperature
monitoring has been activate but the probe is not connecte ).
G. Temperature probe On/Off in icator. Touch the box to activate temperature monitoring. A
check mark will appear in icating that temperature monitoring is activate .
Select the appropriate Amplitu e (intensity setting) for your sample by touching the Up or Down
arrow. Touch Start to begin sonication. Touch Pause to pause sonication.
Manual an Program mo es both show the Start, Pause an Exit buttons. After touching the
Start button an activating sonication, the Start button becomes a Stop button. After touching
the Pause button, the Pause button becomes the Resume button.
If sonication is stoppe , Energy an Elapse Time values remain on isplay. If the Start button
is touche again, the values are reset. If sonication is Pause an Resume , the Energy an
Elapse time ata will resume counting from the point at which it was pause .
The Temperature Probe option can be selecte if you wish to monitor the temperature of the
sample being processe .
B
D
E
G
C
F
A

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Program Menu
The unit can be programme to sonicate at specific, user-selecte time intervals inclu ing pulse
mo e.
Select/Modify a Program – Create, select or mo ify up to 10 ifferent programs.
Select/Modify Sequence 1 & 2 – Select a sequence of programs for the unit to run in
succession. A maximum of 6 programs can be sequence at one time.
Option Screen - The Option menu enables the selection of Temperature units, Footswitch
operational mo e an Display contrast.
To Program
Screen
To
Sequence 2
Screen
To
Sequence 1
Screen
To
Previous
Screen
To Option
Screen

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Programming Screen
How To Create a Program
The ultrasonic processor has the ability to save up to 10 programs.
1. Select a program number from the keypa , the program number will appear above the Amplitu e box.
For each of the following steps: After touching a field, the background will change to yellow indicating
an active field. After entering data, the entry is completed by touching Enter (ENT), touching the field a
second time or by touching a new field.
2. Program a value into the Amplitu e fiel .
3. Program total Process Time (total active sonication time).
4. Program Pulse On time – If no ata is entere (00hr:00min:00sec), the unit will run continuously
without pulsing.
5. Program Pulse Off time – If no ata is entere , the unit will run continuously without pulsing.
Process Time, Pulse On an Pulse Off times are measure in Hours: Minutes: Secon s format.
6. Activate Temperature Monitoring (if necessary).
7. Program a Shut own Temperature (if necessary). Enter a temperature value.
During sonication, if the temperature reaches the Shut own value, the unit will pause sonication. The
unit will remain pause until the temperature rops below the pre-set value. Once the temperature
rops below the value, sonication can be manually resume .
8. Touch Save to store the program to the memory number selecte in step 1.
9. Touch Run to procee to the following screen.
10. Touch Start to begin the program.
How To Run A Saved Program
Program
Progress
Total time of active
sonication that has occurre
uring the current program.
Rest or Off time is not
inclu e in this value.
Total programme time of
active sonication. Rest or
Off time is not inclu e in
this value.