Forma Scientific 1284 User manual

Forma Scientific, Inc.
P.O. Box 649
Marietta, Ohio 45750
U.S.A. Telephone: [phone]
Te1efax: [phone]
Models:
1284, 1285, and 1288
Biological safety Cabinets
Class II, TypeA/B3
Manual No: 7001284 Rev. 0
Read This Instruction Manual
Failure to read, understand and follow the instructions in this manual may result in
damage to the unit, injury to operating personnel and poor equipment performance.
Caution: All internal adjustments and
maintenance must be performedby
qualifiedservicepersonnel.

----------------------------------------------------
Forma Scientific, Inc.
The material in this manual is for information purposes only. The contents and the product it
describes are subject to change without notice. Fonna Scientific Inc. makes no representations or
warranties with respect to this manual.
In no event shall Folllll}. Scientific Inc. be held liable for any
damages, directorincidental, arising out
of or related to the use of this manual
Manual No.
7001284
0 -
--
9/3/97
heg
New manual
REV ECN DATE
BY DESCRIPTION
11

Forma Scientific, Inc. __ -,- _____________________ Safety
General Safety Notes used in this Manual
Alerts the user to important operating and/or maintenance
instructions. May be used alone or with other safety symbols.
Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should
perform the instructions and procedures associated with this
symbol.
Hazard. Do not touch. Instructions associated with this symbol
should only be carried out when using special handing equipment
or when wearing special, protective clothing.
Potential biological hazards. Proper protective equipment and
procedures must be used when following instructions associated
with this symbol.
Reference O.S.H.A. Regulation 1910-1030.
Hot surface(s) present which may cause burns to unprotected
skin or to materials which may be damaged by elevated
temperatures
Potentially hazardous energy. Equipment being maintained or
serviced must be turned off and locked off to prevent possible
injury. Reference O.S.H.A. Regulation 1910-147.
~~I
?'-"I Warning. Skin damage and/or eye injury can be caused by the
light produced by ultra violet light sources installed in this
equipment.
Never work in this unit with the ultra violet light operating.
...J Always use the proper protective equipment (clothing, gloves, goggles etc.).
...J Always dissipate extreme cold orheat, or wear protective clothing.
...J Always follow good hygiene practices.
...J Each individual is responsible for hislher own safety.
111

-------------------------------------
Forma Scientific, Inc. Contents
Table of Contents
Page
Section 1 - Receiving
1.1 Unpacking List....................................................................... 1-1
Section 2 - Introduction
2.1 Description............................................................................. 2-1
2.2 Theory of Operation ............................................................... 2-1
Section 3 - Installation
3.1 Location........................................................................,........ 3-1
3.2 Power Connection .................................................................. 3-1
3.3 Plumbing Connection ............................................................. 3-1
3.4 Exhaust Requirements ............................................................ 3-2 .
a. Direct Room Exhaust ..................................................... 3-2
b. External Exhaust System ............................................... 3-2
3.5 Remote Alarm Contacts, Model 1288 ...................................... 3-3
Section 4 - Operation
4.1 Control and Indicating Devices ............................................... 4-1
a. Blower Switch ..........................:..... ............................... 4-1
b. Light Switch.................................................................. 4-1
c. Ultra-Violet Light (Optional) ......................................... 4-1
d. Alarm By-Pass Switch .................................................. 4-2
e. Static Pressure Gauge (in. w.g.) ..................................... 4-2
f. Blower Speed Control .................................................... 4-2
g. MeasuringBlower Motor Voltage .................................. 4-3 .
h. Blower MotorlLights Reset Button (15 Amp) ................. 4-3
1. Receptacle Fuses (7 amp) ............................................. 4-3
J. Duplex Receptacles................................ ....................... 4-4
v

Forma Scientific, Inc. __________________ Contents
Page
k. Drain Valve................................................................... 4-4
1. Service Valves ................................ ............................... 4-4
m. Exhaust Filter Guard................................ .................... 4-4
n. Sliding Window Assembly................................ .. ........... 4-4
Section 5 - General Cautions
5.1 General Caution Notes ...................................................................... 5-1
Section 6 - Cabinet Start-Up
6. 1 General Recommendations ................................ ................................ 6-1
6.2 Use of Auxiliary Equipment in the Cabinet ........................................ 6-1
6.3 Cabinet Checklist.............................................................................. 6-1
.6.4 Start-Up Procedure................................ ................................ ............ 6-2
Section 7 - Troubleshooting
7.1 Troubleshooting Guide...................................................................... 7-1
Section 8 - Maintenance
8.1 Checking the Static Pressure Gauge "Zero" ............................,........... 8-1
8.2 Re-zeroing the Static Pressure Gauge ................................................ 8-1
8.3 Adjusting the Damper ........................................................................ 8-1
8.4 Biological Safety Cabinet Test Grids ................................................. 8-3
Section 9 - Specifications ...................................................................... 9-1
Section 10 - Accessories ....................................................................... 10-1
Section 11 -Parts List. ............................................................................ 11-1
Section 12 -Schematics ....................................................................... 12-1
Section 13 -Warranty Information .................................................... 13-1
VI

Forma Scientific, Inc. ______________________ .Receiving
Section 1 - Receiving
1.1 Unpacking List
Included with the Installation/Operation manual are four index buttons. These
buttons may be used to identify the type
of service supplied to the service valves. Also
included in a separate bag,
is a small Allen wrench (used for calibrating the Static Pressure
Gauge). This Allen wrench should be kept with the Manual at all times.
1-1

-------------------------------------------
Forma Scientific, Inc. Introduction
Section 2 - Introduction
2.1 Description
_ The 128411285 and 1288 series are Class II, Type AJB3 cabinets. The "Type AlB 3 II
designation indicates two alternative uses of the cabinet. When vented directly into the
laboratory room, the unit serves
as a "Type A" unit. When vented to the outside atmosphere,
through an in-house exhaust system, it serves
as a "Type B3" unit. Either usage of the
cabinet offers both personnel and product protection.
The cabinet can be used in low-to-moderate risk environments and is designed to
NSF, International Standard #49. Class
1,2, and 3 (low-to-moderate risk) agents are
described in the IfBiosafety In Microbiological And Biomedical Laboratories"; CDC NIH
Publication No. (NIH) 88-8395, 3rd Edition, May 1993.
The cabinet's window permits the user to place auxiliary equipment and research
implements in the work area. The work opening mustbe held to 10" during all work
procedures.
If the window is raised higher than the designated 10", the air barrier at the
front
of the cabinet will be weakened and containment will be seriously impaired.
2.2 Theory of Operation
Clean, filtered air descends through the workzone with approximately 40% being
discharged through the exhaust HEPA filter with the remaining air recirculating through the
supply
HEPA filter into the work area. Exhausted air is replaced by rbom air entering the
system through the front access opening.
Room air entering the work zone, through the front access opening, completes the
air barrier
at the unit face and is responsible for the containment properties of the unit. All
work must be performed beyond the intake grille, on the solid work tray.
2-1

Forma Scientific, Inc. ______________________ .Installation
Section 3 - Installation
3.1 Location
Locate the cabinet on a firm level surface in an area of minimum temperature
changes. The cabinet should be placed away from disruptive air currents caused by
excessive personnel traffic, air-conditioning or heating ductwork, or laboratory windows
and doors. Proper cabinet location is important,
as drafts disrupt critical air flow
characteristics and allow room contaminants to enter or escape the cabinetwork area.
Where space permits, fourteen inches should be allowed on each side
of the cabinet
for maintenance, with a minimum
of six inches on the right side of the cabinet for control
panel clearance. A twelve inch height should be available from the top
of the cabinet to the
ceiling.
3.2 Power Connection
The electrical wall outlet(s) leading to the cabinet should be accessible for
electrical testing.
This cabinet is equipped with one power cord supplying power to the blower, lights
and receptacles. The cord should
be plugged into a dedicated circuit.
Refer to Section 9
or to the data plate on the front of the unit for electrical
specifications.
3.3 Plumbing Connection
Two service valves are standard with each cabinet and are located on the right and
left side
of the work station. All service valves are piped within the cabinet. External
connection is to 3/8" FPT coupling. Identification index buttons are supplied.
The cabinet will accommodate six service valves. The additional four service
valves may
be purchased from Forma Scientific, Inc.
Explosive/flammable substances should never be used in
the cabinet, unless approved
and monitored by a biological
safety officer or other qualifiedindividual.
3-1

Forma Scientific, Inc. ______________________ ,Installation
3.4 Exhaust Requirements
Filtered air from the cabinet may be exhausted directly into the room or vented to
the outside through an externalexhaust system. Consult a biological safety officer or other
qualified individual for cabinet-type exhaust requirements.
a. Direct Room Exhaust
• Locate the exhaust filter guard on the top of the cabinet.
• Remove and save the ten acorn-type nuts and washers that secure the exhaust filter
guard.
• Remove the cardboard cover plate from the top
of the cabinet.
• Replace the exhaust filter guard with the slope toward'the front and the back
of the unit.
Secure
it with the ten acorn-type nuts and washers removed earlier.
b. External Exhaust System (For Class II Type 83 Only)
The intermediate connection of the cabinet to any external exhaust system (in-
house) can
be done by using an exhaust transition which is available from Forma Scientific,
Inc. The exhaust transition requires a minimum
of 16-11211 headroom from the top of the
cabinet.
The exhaust system shouldhave safeguards against
exhaustfailure.
It is required that a biological safety officer or
industrial hygienist or other qualifiedindividual review the
agents
and chemicals usedinside the cabinet to determine if
additionalfiltration treatment is necessary before venting to the
atmosphere.
The exhaust system must be capable of handling the 356-390 CFM volume of air
passing through the 1284, 1285 and 1288 cabinets.
3-2

Forma Scientific, Inc.
--------------------------------------------"Installation
3.5 Remote Alarm Contacts (Model 1288 only)
For facilities requiring remote alarms connected to biological safety cabinets, a relay
with Normally Open and Normally Closed contacts is installed in the blower electrical
circuit. The alarm contacts are pre-wired as shown in Figure
3-1 and on the Model 1288
electrical schematic at the back
of this manual.
The relay wires are identified N.O., N.C., and COM and are fitted with quick-connect
terminals with the connector mates installed. The connectors need only to be pulled
apart, then
18 to 22 gauge alarm wiring crimped onto the connectors, and the two halves
pushed back together. The wires may exit the control section
of the cabinetthrough a
hole identified bythe large black arrow in the illustration below.
Refer also to the wiring diagram on the following page to identify the location
of
the remote alarm contact relay.
Figure 3-1
ALARM RElAY 2500l3
128B & 1290
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e

Forma Scientific, Inc. _______________________ Operation
Section 4 - Operation
4.1 Control and Indicating
Devices (Refer To Figures
4-1 and 4-2)
Before operating the cabinet
become familiar with the following
items:
a. Blower Switch: The blower
switch controls power to the internal
blower.
b. Light Switch: The "Lights"
switch controls power
to the
fluorescent lamp
or the optional
ultraviolet lamp (both lamps are
located
in the work area).
c. Ultra-Violet Light (Optional)
Caution: Eyes or
skin should not be
-exposed to ultra-violet
light.
Recommended usage is only when
the laboratory is not in use.
On cabinets equipped with the
optional ultra-violet germicidal light,
the dual purpose "Lights" switch
provides the following settings:
"off" = center
"on" top = fluorescent lamp
"on" bottom ultra-violet lamp
External Damper
Control
Exhaust filter ~
grille
Speed control
Front View
Sliding window
wlsafety glass
Removable vortex
channel
~) L:§''-=§'~=-=:'.E...EE,-=EO!L-''''---:l~----i
-- -- -- - -- -- -.:::- --ii
access panel.
spare Fuses on
under-side
Dwyer, 0" - 2"
mini-helic gauge
Drain valve -----?
--=3:1'4- Exhaust HEPA filter
.....
k----.l~_LSupply HEPA filter
JiP:=~=~~9-Air diffuser screen
Iii!!ll (If.;;
standard duplex
outlets, one
on
each side
Figure 4-1
Series 1284 Class II A/B3 Biological Safety Cabinet
Blower housing with
Side View directdrive motor
18"x 24" x 6"
18"x 48" x 6"
Extra valve ports,
two on each side
standard key cocks,
one on each side
Removable rear
paper
catch
4-1

Forma Scientific, Inc. _______________________ Operation
Either thefluorescent lamp or the ultra-violent lamp may be lit at one time.
d. Ala~m By-Pass Switch
The Alarm By-Pass switch silences the audible "Window Above 10 Inch" alarm for
five minutes. The red visual indicator remains illuminated. The alarm rings-back to remind
the operator that the window is still open more than
10 inches.
e. Static Pressure Gauge (In. W.G.)
on
o
WlfldoW
above- 10 In.
Figure 4-2
The static pressure
g~uge
measures the air pressure differential
across the filters providing an indication
of filter "loadingtl. As the filters become
loaded, resistance increases and the
reading on the static pressure gauge
increases accordingly. When the reading
increases by 50% from original
measurement, cabinet airflow should be
checked with a thermoanemometer.
Replace the filters
if proper airflow cannot
be obtained.
The staticpressure gauge should not be used as a direct measure of airflow.
f. Blower Speed Control
The blower speed control is accessed from the front of the control panel by
removing the access panel. The blower speed is adjusted by turning the screw on the
variable resistor mounted on the circuit board adjacent to the controller. (Refer to Figure
4-3) Turning the screw clockwise increases air velocity; counter-clockwise decreases it.
The blower speedisfactory set and shouldonly be changedby a qualified
technician.
Live voltage ispresent on the control terminals
of the switches
and dials on the front of the blowerpanel. Avoid touching these
controls when reaching into the control
panel and making any
adjustments.
4-2

Forma Scientific, Inc. ______________________ Operation
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g. Measuring Blower Motor
Voltage
Both blower motor voltage and line
voltage are measured at the three
terminal connectors at the top
of the
circuit board. Refer to Figure 4-4.
h. Blower Motor/Lights Reset
Button (15 Amp)
The reset button (located on
the left side
of the control panel,
directly above the Receptacle fuses) is
an inline circuit breaker for the
internal blower motor and lighting.
If
an overload occurs, the circuit breaker
will trip, and the button will protrude
from the panel. Depressing the button
resets the circuit breaker.
Voltage to
Blower Motor
115 VAC Une
Neutral Voltage
®
Note: • Turn power off to the blower.
• Turn power
off to the
lighting.
• Press the blower motor/lighting
reset button.
Figure 4-4
i. Receptacle Fuses (7 Amp)
The receptacle fuses (2) located directly below the Blower MotorlLights Reset
Button, are for the receptacles only.
If an overload occurs the fuses will open and will
require replacement.
Note: • Turn the power off.
• Unplug the unit;
• Replace the fuses -Forma PIN 230182, 7 Amp,
'i4" x 1 Y4"
(Two spare fuses are located in a bracket on the back of the speed
control access panel, upperright corner
of the front of the
cabinet. Refer to Figure
4-1 )
4-3

Forma Scientific, Inc. _______________________ Operation
j. Duplex Receptacles
Duplex receptacles (115 volts) are located on the left and right side wall of the
workstation. Power
is controlled by the receptacle switch located on the control panel. The
maximum load is 5 amps totaL The model 1285 is equipped with 2 single European 230V
receptacles.
k. Drain Valve
The drain valve, located on the right front side of the cabinet, is provided for the
safe drainage
of the drain pan. This valve must remain closed while work is being
performed in the cabinet and be used only in the event
of a major spill.
If a spill occurs, immediately consult a biological safety officer or
other qualifiedindividual
for properprocedures. To contain a spill,
connect a sealed hose
from the drain valve to a sealed container.
I. Service Valves
Two service valves are standard with each cabinet. These valves are located on the
right and left side
of the work station and can be coded with the type of service that they
supply. Identification index buttons are supplied.
The cabinet supports six service valves. Additional valves may be purchased from
Forma Scientific Inc.
m. Exhaust Filter Guard
The exhaust filter guard, located on top of the exhaust filter, protects the exhaust
air flow and prevents the storing
of objects on top of the housing.
n. Sliding Window Assembly
The sliding window assembly allows the operator to raise the glass window to place
items within the work area.
If the sliding window is above the 10" level, a red light and
audible alarm warns that an unsafe condition exists.
When work is beingperformed in the cabinet, the sliding windOw
must be at the 10" position to avoidcontamination to product and
personnel.
4-4

Forma Scientific, Inc. _______________________ Operation
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4-5

Forma Scientific, Inc. ____________________ General Cautions
Section 5 - General Cautions
5.1 Caution Notes
• Following initial installation, the unit must be thoroughly tested and certified.
• All activities to be performed within the cabinet should be approved by a biological
safety officer or other qualified individual.
• Since the HEPA filters remove particulates only (not gas), explosive/flammable
substances should never be used in the cabinet, unless approved and monitored by a
biological safety officer or other qualified individual.
• Ultra-violet lighting should not be used while personnel are using the cabinet.
If
exposure cannot be avoided the proper safety gear/clothing must be worn. Consult a
biological safety officer or other qualified individual for proper procedures.
•
If the cabinet is to be used for biological or toxicological applications, it must be
monitored by a biological safety officer
or other qualified individual.
• If the unit needs to be serviced, it must be decontaminated to protect service personnel
from contamination. The cabinet must then be recertified by a qualified certifying
agency.
• None
of the perforations in the work area may be covered or blocked, as air flow will
be disrupted and contamination may occur.
• Paper catches should always be kept free
of debris
5-1

Forma Scientific, Inc. _______________________ Start-Up
Section 6 - Cabinet Start-Up
6.1 General Recommendations
• Keep the movement i~ the room to a minimum when the cabinet is in use.
• Keep all laboratory doors closed to prevent drafts that may disturb critical air flow.
• Pre-plan cabinet use and place everything needed in the cabinet so that nothing passes
through the air barrier (in or out) during the procedure.
• Practice good aseptic technique to insure safe use
of the cabinet.
• If a spill occurs, clean it up immediately. Decontaminate the work area and all
affected equipment.
• Do not cover or block the exhaust grille.
• Do
not cover or block any perforations (air holes) in the work area.
6.2 Use of Auxiliary Equipment in the Cabinet
Use auxiliary equipment in the cabinet only if proper precautions are taken.
Appliances used in the work area will cause turbulence anddisturb the air flow and should
be carefully managed. The equipment should be placed
at the rear of the work space where
it will have minimal effect.
Blender: A blender may be used
in the cabinet. But because of the amount of
aerosol it produces and the turbulence it causes, it is recommended that it be removed from
the cabinet as soon as possible.
6.3 Cabinet Checklist
• Verify that the Drain Valve is closed (the handle is horizontal).
6-1

Forma Scientific, Inc. _______________________ Start-Up
• Verify that all service valves are closed.
• Verify that the cardboard exhaust filter protector has been removed.
6.4 Start-Up Procedure
• Turn the light on.
• Check the intake and exhaust grills to ensure they are not blocked.
• Turn the blower on.
• Place everything needed into the cabinet.
• Place the viewing window at
10"
6-2

Forma Scientific, Inc. ____________________ Troubleshooting
Section 7 - Troubleshooting
7.1 Troubleshooting Guide
The following is a guide to troubleshooting the system.
If a contaminated area of
the cabinet must be entered to determine and/or resolve the source of a particular problem,
the cabinetmust first be decontaminated
Servicing
of the unit must be performed by qualified servicepersonnel.
Problem 1: Air flow in the cabinet work area and through the exhaust filter is inadequate.
Possible causes:
• Exhaust filter is blocked by laboratory materials or the protective shipping cover.
•
If the biological safety cabinet is connected to an exhaust system, there may be
inadequate exhaust suction or back pressure
inthe duct system. The system must be
rebalanced to handle the correct air volume. A Biological Safety Officer should be
consulted.
• Low voltage being applied to the blower motor.
• Blower motor or speed control is defective.
• Supply HEPA filter and Exhaust HEPA filter may be loaded. Decontaminate the unit
and replace bothHEPA filters.
Before any maintenance work isperformed in the biological safety cabinet,
the unit must first be decontaminated.
Problem 2: Ultra violet light malfunction
Possible causes:
• Check lamp pins and socket ends for contact.
• Defective starter for the
UN light.
7-1