GE akta start User manual

ÄKTA™start
Maintenance Manual
Original instructions

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Table of Contents
51 Introduction ..........................................................................................................
61.1 About this manual ................................................................................................................................
71.2 Important user information .............................................................................................................
91.3 Regulatory information ......................................................................................................................
121.4 Associated documentation ..............................................................................................................
152 Safety instructions ...............................................................................................
162.1 Safety precautions ...............................................................................................................................
242.2 Labels .........................................................................................................................................................
282.3 Emergency procedures ......................................................................................................................
302.4 Recycling procedures .........................................................................................................................
312.5 Declaration of Hazardous Substances (DoHS) ........................................................................
343 System description ..............................................................................................
353.1 System overview ...................................................................................................................................
393.2 Instrument ...............................................................................................................................................
424 Unpack ÄKTA start and Frac30 ..........................................................................
434.1 Unpack ÄKTA start ................................................................................................................................
484.2 Unpack Frac30 .......................................................................................................................................
544.3 Accessories package ..........................................................................................................................
555 Service and settings ............................................................................................
565.1 Preventive maintenance ...................................................................................................................
585.2 Clean before planned maintenance or service ......................................................................
595.3 Access the modules .............................................................................................................................
625.4 UV Monitor ...............................................................................................................................................
645.4.1 UV LED calibration .........................................................................................................................
665.4.2 Diagnostics .......................................................................................................................................
695.4.3 Flow cell path length ....................................................................................................................
725.4.4 Reset number of run hours ........................................................................................................
745.5 Pump ..........................................................................................................................................................
765.5.1 Diagnostics .......................................................................................................................................
785.5.2 Calibration .........................................................................................................................................
805.5.3 Pump tubing log .............................................................................................................................
825.6 Buffer valve ............................................................................................................................................
855.7 Sample valve ...........................................................................................................................................
885.8 Wash valve ..............................................................................................................................................
915.9 Outlet valve ..............................................................................................................................................
945.10 Conductivity Monitor ...........................................................................................................................
955.10.1 Set the Cell constant value .........................................................................................................
975.10.2 Temperature sensor calibration ..............................................................................................
985.10.3 Conductivity flow cell calibration ............................................................................................
1015.11 Pressure sensor ....................................................................................................................................
ÄKTA start Maintenance Manual 29-0603-08 AC 3
Table of Contents

1035.12 Frac30 ........................................................................................................................................................
1045.12.1 Enable or disable Frac30 ............................................................................................................
1055.12.2 Diagnostics .......................................................................................................................................
1075.12.3 Run Log ..............................................................................................................................................
1095.13 Instrument Display ...............................................................................................................................
1115.13.1 Touch screen calibration .............................................................................................................
1145.13.2 Color test ............................................................................................................................................
1155.13.3 Diagnostics .......................................................................................................................................
1165.13.4 Log book ............................................................................................................................................
1185.14 System .......................................................................................................................................................
1205.14.1 Firmware update ............................................................................................................................
1235.14.2 Export system report to USB .....................................................................................................
1245.14.3 Delay volume setting ....................................................................................................................
1255.14.4 Switch valve timing .......................................................................................................................
1275.15 Main board ...............................................................................................................................................
1286 Troubleshooting ..................................................................................................
1296.1 UV .................................................................................................................................................................
1326.2 Conductivity ............................................................................................................................................
1336.3 Frac30 ........................................................................................................................................................
1346.4 Pump ..........................................................................................................................................................
1356.5 Pressure sensor .....................................................................................................................................
1366.6 Main board and Power Supply .......................................................................................................
1386.7 System related error messages .....................................................................................................
1396.8 Troubleshooting flow charts ............................................................................................................
1457 Disassembly ..........................................................................................................
1497.1 Buffer valve ..............................................................................................................................................
1537.2 Mixer ...........................................................................................................................................................
1567.3 UV .................................................................................................................................................................
1617.4 UV flow cell ..............................................................................................................................................
1637.5 Sample valve ...........................................................................................................................................
1677.6 Pump ..........................................................................................................................................................
1727.7 Pump tubing ............................................................................................................................................
1757.8 Wash valve ..............................................................................................................................................
1797.9 Outlet valve ..............................................................................................................................................
1827.10 Conductivity Monitor ...........................................................................................................................
1867.11 Injection valve ........................................................................................................................................
1897.12 Injection valve kit ..................................................................................................................................
1927.13 Frac30 Bowl assembly .......................................................................................................................
2007.14 Fuse .............................................................................................................................................................
2038 Reference information ........................................................................................
2048.1 Health and Safety Declaration Form ...........................................................................................
2068.2 Ordering information ..........................................................................................................................
210Index .......................................................................................................................
4 ÄKTA start Maintenance Manual 29-0603-08 AC
Table of Contents

1 Introduction
About this chapter
This chapter contains important user information, description of safety notices, regula-
tory information, intended use of ÄKTA start, and list of associated documentation.
In this chapter
See pageSection
61.1 About this manual
71.2 Important user information
91.3 Regulatory information
121.4 Associated documentation
ÄKTA start Maintenance Manual 29-0603-08 AC 5
1 Introduction

1.1 About this manual
Purpose of this document
The ÄKTA start Maintenance Manual provides you with the instructions needed to unpack,
maintain and troubleshoot ÄKTA start in a safe way.
Nomenclature conventions
The nomenclature used in this manual are explained in the table below.
ExplanationConcept
The instrument.ÄKTA start
The Fraction collector.Frac30
The software installed on a computer.UNICORN™ start
The entire liquid chromatography system, including instrument,
Fraction collector and software.
ÄKTA start System
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (for example, Buffer valve).
6 ÄKTA start Maintenance Manual 29-0603-08 AC
1 Introduction
1.1 About this manual

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use
ÄKTA start is a liquid chromatography system used for preparative purification of proteins
at laboratory-scale. The system can be used for a variety of research purposes to fulfill
the needs of the users in the academia and in the life sciences industry.
ÄKTA start is intended for research use only, and shall not be used in any clinical proce-
dures, or for diagnostic purposes.
Prerequisites
In order to follow this manual and use the system in the manner it is intended, it is im-
portant that:
•you understand the concepts of liquid chromatography
•you have read and understood the Safety instructions chapter in the ÄKTA start
Maintenance Manual.
ÄKTA start Maintenance Manual 29-0603-08 AC 7
1 Introduction
1.2 Important user information

Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
8 ÄKTA start Maintenance Manual 29-0603-08 AC
1 Introduction
1.2 Important user information

1.3 Regulatory information
Introduction
This section describes the directives and standards that are fulfilled by ÄKTA start.
Manufacturing information
The table below summarizes the required manufacturing information. For further infor-
mation, see the EC Declaration of Conformity (DoC) document.
ContentRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala,
Sweden
Conformity with EU Directives
This product complies with the European directives listed in the table below, by fulfilling
the corresponding harmonized standards. For further information, see the EC Declaration
of Conformity document.
TitleDirective
Machinery Directive (MD)2006/42/EC
Electromagnetic Compatibility (EMC) Directive2004/108/EC
Low Voltage Directive (LVD)2006/95/EC
ÄKTA start Maintenance Manual 29-0603-08 AC 9
1 Introduction
1.3 Regulatory information

CE marking
The CE marking and the corresponding EC Declaration of Conformity is valid for the in-
strument when it is:
•used as a stand-alone unit, or
•connected to other products recommended or described in the user documentation,
and
•used in the same state as it was delivered from GE, except for alterations described
in the user documentation.
International standards
This product fulfills the requirements of the following standards:
NotesDescriptionStandard
EN standard is
harmonized with EU
directive 2006/95/EC
Safety requirements for
electrical equipment for mea-
surement, control, and labora-
tory use.
EN 61010-1, IEC
61010-1, UL 61010-1,
CAN/CSA C22.2 No.
61010-1
EN standard is
harmonized with EU
directive 2004/108/EC
Electrical equipment for
measurement, control and
laboratory use - EMC require-
ments
EN 61326-1, IEC
61326-1
(Emission according to
CISPR 11, Group 1,
class A)
EN ISO standard is
harmonized with EU
directive 2006/42/EC
Safety of machinery. General
principles for design. Risk
assessment and risk reduction.
EN ISO 12100
WARNING
This is a Class A product. In a domestic environment, this product
may cause radio interference, in which case the user may be re-
quired to take adequate measures.
10 ÄKTA start Maintenance Manual 29-0603-08 AC
1 Introduction
1.3 Regulatory information

FCC statement
ÄKTA start complies with FCC 47 CFR Part 15b.
This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to part 15 of the FCC Rules. These limits are designed
to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment generates,
uses and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to
radio communications. Operation of this equipment in a residential area is
likely to cause harmful interference in which case the user will be required to
correct the interference at his own expense.
Note:
Regulatory compliance of
connected equipment
Any equipment connected to ÄKTA start should meet the safety requirements of EN
61010-1/IEC 61010-1, or relevant harmonized standards. Within EU, connected equipment
must be CE marked.
Environmental Conformity
DescriptionRequirement
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Administration on the Control of Pollution Caused by Electronic In-
formation Products, China Restriction of Hazardous Substances
(RoHS)
ACPEIP
ÄKTA start Maintenance Manual 29-0603-08 AC 11
1 Introduction
1.3 Regulatory information

1.4 Associated documentation
Introduction
This section describes the user documentation that is delivered with the instrument and
how to find related literature that can be downloaded or ordered from GE.
User documentation on the CD
The user documentation listed in the table below is available on the ÄKTA start User
Documentation CD. A display help is available from the Instrument Display.
Main contentsDocumentation
Instructions needed to install, operate
and maintain ÄKTA start in a safe way.
ÄKTA start Operating Instructions
Dialog descriptions of the functionality
menu for ÄKTA start (only accessible from
the Instrument Display).
ÄKTA start Instrument Display Help
Detailed instrument and module descrip-
tions, including instructions needed to
maintain and troubleshoot ÄKTA start.
ÄKTA start Maintenance Manual
A condensed guide to prepare and run
chromatographic techniques on ÄKTA
start.
ÄKTA start System Cue Card
A condensed guide to handling routine
maintenance operations and trou-
bleshooting ÄKTA start.
ÄKTA start Maintenance Cue Card
12 ÄKTA start Maintenance Manual 29-0603-08 AC
1 Introduction
1.4 Associated documentation

From the Help menu in UNICORN start or on the UNICORN start DVD, the following user
documentation is available.
Main contentsDocumentation
Overview and detailed descriptions of the
system control software designed for
ÄKTA start, which includes process pic-
ture map for real time monitoring,
method editor, evaluation and adminis-
tration modules.
UNICORN start 1.0 User Manual
Dialog descriptions for UNICORN start
(only accessible from the Help menu).
UNICORN start Online Help
Data files, application notes and
user documentation on the web
To order or download data files, application notes or user documentation, see the instruc-
tion below.
ActionStep
Go to www.gelifesciences.com/AKTA.1
Click ÄKTA Lab-Scale Systems.2
Select ÄKTA start from the list.3
Click Related Documents.4
Select to download the chosen literature.5
ÄKTA start Maintenance Manual 29-0603-08 AC 13
1 Introduction
1.4 Associated documentation

Access documentation from
mobile units
Scan the code using your mobile phone or tablet computer to access the product page
for ÄKTA start. Select documents to download under the Related Documents tab.
14 ÄKTA start Maintenance Manual 29-0603-08 AC
1 Introduction
1.4 Associated documentation

2 Safety instructions
About this chapter
This chapter describes safety precautions and emergency shutdown procedures for the
product. The labels on the system and recycling procedures are also described.
In this chapter
This chapter contains the following sections:
See pageSection
162.1 Safety precautions
242.2 Labels
282.3 Emergency procedures
302.4 Recycling procedures
312.5 Declaration of Hazardous Substances (DoHS)
ÄKTA start Maintenance Manual 29-0603-08 AC 15
2 Safety instructions

2.1 Safety precautions
Introduction
ÄKTA start is powered by mains voltage and handles liquids that may be hazardous.
Before installing, operating or maintaining the system, you must be aware of the hazards
described in this manual. Follow the instructions provided to avoid personal injuries
or damage to the equipment.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Using flammable liquids
•Personal protection
•Installing and moving the instrument
•System operation
•Maintenance
General precautions
WARNING
Always follow these General precautions to avoid injury when using
ÄKTA start.
•Do not operate ÄKTA start in any other way than described in
the ÄKTA start user documentation.
•Operation and user maintenance of ÄKTA start should be per-
formed according to the instructions described in ÄKTA start
Operating Instructions and ÄKTA start Maintenance Manual.
•Do not use any accessories not supplied or recommended by
GE.
•Do not use ÄKTA start if it is not working properly, or if it has
suffered any damage, for example:
-damage to the power cord, its plug or the Frac30 cable
-damage caused by dropping the equipment
-damage caused by splashing liquid onto it
16 ÄKTA start Maintenance Manual 29-0603-08 AC
2 Safety instructions
2.1 Safety precautions

Using flammable liquids
WARNING
When using flammable liquids with ÄKTA start, follow these precau-
tions to avoid any risk of fire or explosion.
•Fire Hazard. Before starting the system, make sure that there
is no unintentional leakage in the instrument or tubing.
•Explosion hazard. To avoid building up an explosive atmo-
sphere when using flammable liquids, make sure that the room
ventilation meets the local requirements.
CAUTION
To avoid hazardous situations when unpacking, installing or moving
ÄKTA start, follow the precautions below.
ÄKTA start is filled with denaturated alcohol (50% C2H5OH (ethanol))
at delivery. The denatured alcohol mixture can be hazardous to
humans if consumed. Flush out the denaturated alcohol before
assembling, testing or integrating ÄKTA start into the intended
process context.
Personal protection
WARNING
To avoid hazardous situations when working with ÄKTA start, take
the following measures for personal protection.
Spread of biological agents. The operator has to take all necessary
actions to avoid spreading hazardous biological agents in the
vicinity of the equipment. The facility should comply with the na-
tional code of practice for biosafety.
ÄKTA start Maintenance Manual 29-0603-08 AC 17
2 Safety instructions
2.1 Safety precautions

CAUTION
To avoid hazardous situations when working with ÄKTA start, take
the following measures for personal protection.
•Always use appropriate personal protective equipment during
operation and maintenance of ÄKTA start.
•Spillage hazard. When using ÄKTA start use personal protective
equipment like goggles, lab coat, protective shoes and gloves
to avoid any circumstances of spillage.
•Cut injuries. The tubing cutter is very sharp and must be han-
dled with care to avoid injuries.
•Hazardous substances. When using hazardous chemical and
biological agents, take all suitable protective measures, such
as wearing protective glasses and gloves resistant to the sub-
stances used. Follow local and/or national regulations for safe
operation, maintenance and decommissioning of the equip-
ment.
18 ÄKTA start Maintenance Manual 29-0603-08 AC
2 Safety instructions
2.1 Safety precautions

Installing and moving the
instrument
WARNING
To avoid damage to person when installing or moving ÄKTA start,
follow the instructions below.
•Moving the instrument horizontally. One person is recommend-
ed when moving the instrument horizontally.
•Supply voltage. Make sure that the supply voltage at the wall
outlet corresponds to the marking on the instrument, before
connecting the power cord.
•Power cord. Only use grounded power cords delivered or ap-
proved by GE.
•Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power
switch must always be easy to access. The power cord with
plug must always be easy to disconnect.
•Installing the computer (optional). The computer should be
installed and used according to the instructions provided by
the manufacturer of the computer.
•Disconnect power. Always switch off power to ÄKTA start be-
fore an instrument module is removed or installed, or a cable
is connected or disconnected.
CAUTION
To avoid damage to person when installing or moving ÄKTA start,
follow the instructions below.
Protective ground. ÄKTA start must always be connected to a
grounded power outlet.
ÄKTA start Maintenance Manual 29-0603-08 AC 19
2 Safety instructions
2.1 Safety precautions

NOTICE
To avoid damage to ÄKTA start or other equipment when installing
or moving the instrument, follow the instructions below.
•Vents on ÄKTA start. To ensure adequate ventilation, keep
papers and other objects away from the vents of the instru-
ment.
•Any computer used with the equipment shall comply with IEC
60950 and be installed and used according to the manufactur-
er's instructions.
•Frac30 should not be connected or disconnected from ÄKTA
start when the instrument is powered ON.
System operation
WARNING
To avoid personal injury when operating ÄKTA start, follow the in-
structions below.
•Rotating the instrument. Make sure that there is always at
least 20 cm of free space around ÄKTA start to allow for suffi-
cient ventilation. When turning or moving the instrument, take
care not to stretch or squeeze tubing or cables. A disconnected
cable may cause power interruption or network interruption.
Stretched tubing may cause bottles to fall, resulting in liquid
spillage and shattered glass. Squeezed tubing may cause in-
crease in pressure, or block liquid flow. To avoid the risk of
knocking over bottles, always place bottles on the buffer tray
and turn or move carefully.
•Hazardous chemicals during run. When using hazardous
chemicals, run the System cleaning template to clean and flush
the entire system tubing with distilled water, before service and
maintenance.
•Setting. Check that the correct outlet size settings are used.
Make sure that tubing and fittings are properly connected and
secured. Make sure that the pressure limit settings are correct
before starting the run.
20 ÄKTA start Maintenance Manual 29-0603-08 AC
2 Safety instructions
2.1 Safety precautions