GE AKTA Valve INV-907 User manual

Valve INV-907
Instructions
GE Healthcare
ÄKTA™

Important user information
All users must read this entire manual to fully
understand the safe use of Valve INV-907.
WARNING!
The WARNING! sign highlights instructions
that must be followed to avoid personal injury.
It is important not to proceed until all stated
conditions are met and clearly understood.
CAUTION!
The Caution! sign highlights instructions that
must be followed to avoid damage to the
product or other equipment. It is important not
to proceed until all stated conditions are met
and clearly understood.
Note
The Note sign is used to indicate information
important for trouble-free and optimal use of
the product.
CE Certifying
This product meets the requirements of applicable CE-
directives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corresponding declaration of
conformity, is valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked GE Healthcare
instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from GE
Healthcare except for alterations described in this
manual.
Recycling
This symbol indicates that the waste of
electrical and electronic equipment must not
be disposed as unsorted municipal waste
and must be collected separately. Please
contact an authorized representative of the
manufacturer for information concerning the
decommissioning of equipment.
WARNING!
This is a Class A product. In a domestic environment
this product may cause radio interference in which
case the user may be required to make adequate
measures.

4Valve INV-907 Instructions 56-8101-01 Edition AE

Valve INV-907 Instructions 56-8101-01 Edition AE
Contents
1 Introduction ...................................................................................................................... 7
2 Installation
........................................................................................................................... 9
3 Operation
.......................................................................................................................... 11
4 Maintenance
............................................................................................................. 15
5 Trouble-shooting
............................................................................................ 17
6 Reference information
..................................................................... 19
5

6Valve INV-907 Instructions 56-8101-01 Edition AE

Introduction 1
7
Valve INV-907 Instructions 56-8101-01 Edition AE
1 Introduction
Valve INV-907 is a motorised rotary 7–port valve. The valve is used in
ÄKTA™design chromatography systems. It is powered from the ÄKTAdesign
P-900 series system pump, and is controlled from UNICORN™ control
system.
The valve has 3 positions used for:
- loading a sample loop
- injecting the sample onto the column
- washing the system pump.
The valve can also be used to switch between upflow and downflow in a
column.
Features:
• Completely swept flow path minimises eluent or sample ”memory
effect”.
• Flow rates up to 100 ml/min.
• All wetted parts are plastic PEEK.
Safety
WARNING! When using hazardous chemicals, take all suitable protective
measures, such as wearing protective glasses and gloves resistant to
the chemicals used. Follow local regulations and instructions for safe
operation and maintenance of the system.

8Valve INV-907 Instructions 56-8101-01 Edition AE
1 Introduction

2 Installation
Installation 2
9
Valve INV-907 Instructions 56-8101-01 Edition AE
CAUTION! Before connecting Valve INV-907 ensure the power is switched
OFF at the system pump or the complete system.
Unpacking
Unpack the valve and check the items against the packing list. Inspect the
items for obvious damage which may have occurred during transportation.
Installing the valve
1 Connect the valve with two UniNet cables as
a part of the UniNet 2 chain.
2 Set the ID-switch at the bottom to the required
valve number 0–9. The number should correspond
to that used in UNICORN.
Note: All valves must have different numbers.
3 Mount the valve vertically, in the way it is shown in the user
documentation of your ÄKTAdesign chromatography system.
4 Apply an identification number from the labels supplied. The number
should be the same as that of the rotary switch at the bottom of the
valve. Place the number so that it is easily readable from the front.
5 Connect the tubing.
INV-907
3
Uninet 2 network

2 Installation
10 Valve INV-907 Instructions 56-8101-01 Edition AE
6 When used as an injection valve connect the tubing as follows:
Port Connect to
1 Column inlet
2 Sample loop or bottom tubing of Superloop
3 Injection port
4 Waste
5 Waste
6 Sample loop or top tubing of Superloop
7 Outlet of the pump
7 When used as an upflow/downflow valve, connect the valve as indicated
in the user documentation of your ÄKTAdesign chromatography system.

Operation 3
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Valve INV-907 Instructions 56-8101-01 Edition AE
The valve is controlled from UNICORN. When used as an injection valve use
the instruction InjectionValve in System Control:Manual:Flowpath. The
valve can be set in 3 positions:
• LOAD position 1 (home position)
• INJECT position 2
• WASTE position 3
If the valve has not been activated it is in position 1.
3 Operation
1
2
3
45
6
7
1
2
3
4
5
6
7
1
2
3
45
6
7
LOAD, position 1
INJECT, position 2
WASTE, position 3
Column
Sample
syringe
Pump
Waste
Column
Pump
Waste
Column
Pump
Waste
Waste

12 Valve INV-907 Instructions 56-8101-01 Edition AE
3 Operation
Manual sample loading
The sample loop and Superloop can be loaded with a syringe. Connect
Superloop with the bottom tubing connected to port 2 and the upper tubing
to port 6.
For large volumes use a luer female/1/16” male union
connected to port 3.
For smaller volumes use an injection needle together
with the injection fill port as described below:
Check the fill port nozzle is screwed into the fill port.
Note: The thread is left-handed.
1 Loosely thread the fill port screw into valve port 3.
2 Insert the injection needle (0.7 mm o.d.) into the injection fill port.
3 Tighten the fill port until the nozzle has formed a seal around the needle
tip. When the seal is adjusted correctly, it feels as if you are penetrating
a septum at the end of the injection fill port. The seal should provide
easy insertion and removal of the needle.
4. Mount the syringe holder on the fill port.
INV-907
3

Operation 3
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Valve INV-907 Instructions 56-8101-01 Edition AE
Two techniques can be used for filling the sample loop; partial or complete
filling.
Type of filling Volume to load
Partial filling max 50% of the sample loop volume
Complete filling 2–5 times the sample loop volume
Partial Filling
Partial filling is used when high recovery is required. Partial filling allows
the injected volume to be changed without changing the loop and does not
waste sample.
Partial filling is achieved as follows:
1 Set injection valve to position LOAD.
2 Load the syringe with a large volume of buffer (5× the loop volume).
3 Fill the sample loop carefully with buffer.
4 Set the injection valve to position INJECT before taking out the syringe.
Note: If the syringe is taken out when the injection valve is in position
LOAD, self drainage will occur and air will enter the sample loop.
5 Load the syringe with the required volume of sample. No more than half
(50%) a loop volume of sample should be loaded into the loop.
6 Insert the syringe in position 3 on the injection valve. Set the injection
valve to position LOAD.
Note: Do not load the sample before the valve is in position LOAD.
7 Gently load the syringe contents into the loop.
8 Leave the syringe in position. The sample will be injected onto the
column when the valve is switched to INJECT in the method.

3 Operation
14 Valve INV-907 Instructions 56-8101-01 Edition AE
Complete filling
In this method a surplus of sample is used to ensure that the sample loop
is filled completely, allowing reproducible sample volumes. In preparative
applications the sample volume should be at least 2 times the volume of
the sample loop. For analytical reproducibility a sample volume 5 times the
volume of the sample loop should be used.
With complete filling the sample volume can only be changed by changing
the loop size.
Complete filling is achieved as follows:
1 Set valve to position LOAD.
2 Load the syringe with sample (2-5 times the loop volume).
3 Gently load the syringe contents into the loop.
4 Leave the syringe in position. The sample will be injected onto the
column when the valve is switched to INJECT in the method.
Note: If the syringe is taken out before the sample is injected onto the
column, self drainage will occur and the loop will be emptied.
Emptying the sample loop
When emptying the sample loop a buffer volume of approx. 5 times the
sample loop volume should be used to flush the loop and ensure that all
sample is injected onto the column.
Storage
Overnight: The valve can be left filled with a buffer.
Weekend or Long time storage: Flush the valve with water and then fill it
with 20% Ethanol.

4 Maintenance
Maintenance 4
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Valve INV-907 Instructions 56-8101-01 Edition AE
WARNING! When using hazardous chemicals, take all suitable protective
measures, such as wearing protective glasses and gloves resistant to
the chemicals used. Follow local regulations and instructions for safe
operation and maintenance of the system.
CAUTION! Only spare parts approved or supplied by GE Healthcare may
be used for maintaining and servicing the valve.
Period Action
Every 12 months Change channel plate and
or when required distribution plate
Cleaning-in-place
Pump a cleaning or sanitizing agent through the valve. The standard
recommendation is to pump 1 M NaOH for 30 minutes and then wash out
with buffer.
Changing channel plate and distribution plate
WARNING! NaOH is injurious to health. Avoid spillage.
A replacement kit, Valve Kit INV–907, is available, see ordering information in
Reference Information.
1 Ensure that the valve is in position 1 and then disconnect it from the
pump.
2 Remove the 4 screws on the front using the supplied 3 mm Allen key.
Loosen each one equally in turn so the distribution plate comes off
parallel to the valve body.
3
INV-907
Channel plate
Channel plate

16 Valve INV-907 Instructions 56-8101-01 Edition AE
4 Maintenance
3 Slide the screws out.
4 Remove the distribution plate containing the ports.
5 Remove the old channel plate and insert a new one.
6 Remount a new distribution plate so that the text 3 is horizontal and to
the right of the central tubing connection. Using the Allen key, tighten
the 4 screws in turn, a little at a time, until the distribution plate is fixed
to the valve body.
This symbol indicates that the waste of electrical and electronic equipment
must not be disposed as unsorted municipal waste and must be collected
separately. Please contact an authorized representative of the manufacturer
for information concerning the decommissioning of equipment.

Troubleshooting 5
17
Valve INV-907 Instructions 56-8101-01 Edition AE
If the suggested actions do not correct the fault, call GE Healthcare.
Fault Action
The valve is not switching
1 Check the connection to the pump.
The valve should be connected to the UniNet
2 socket, not the UniNet 1 socket.
2 Check the ID-switch on the valve.
The ID number should correspond to the
number set in UNICORN.
3 Check the UniNet cable and replace if
required.
The valve is switching to wrong position
The valve parts may have been incorrectly
assembled after replacement.
1 Check that the distribution plate marking 3 is
horizontal.
External leakage
1 Check the tubing connections.
Tighten or replace if required.
Internal leakage
Internal leakage are easy to detect at the
small hole on the underside of the valve body.
1 Internal valve parts may be worn.
Change channel plate and distribution plate
according to section 4.
High back pressure (clogging)
1 Do cleaning-in-place according to the
instructions in section 4.
2 Change channel plate and distribution plate
according to section 4.
Other faults Contact GE Healthcare.
This symbol indicates that the waste of electrical and electronic equipment
must not be disposed as unsorted municipal waste and must be collected
separately. Please contact an authorized representative of the manufacturer
for information concerning the decommissioning of equipment.
5 Troubleshooting

18 Valve INV-907 Instructions 56-8101-01 Edition AE
5 Troubleshooting

Reference information 6
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Valve INV-907 Instructions 56-8101-01 Edition AE
Description
The valve consists of two main parts:
• Housing which
encloses the motor
and electronics.
• Valve body with a
rotating central core.
As the channel plate is turned by
the motor, different ports are connected.
Valve switching is controlled from UNICORN by reading the actual position
of the channel plate.
The geometry of the valve assures that the flow path is completely swept so
that solvent or sample ”memory effect” is virtually non-existing. The valve
rotates the shortest way to the next position (max. 180°). The material used
in the switching parts ensures both long mechanical and chemical lifetime.
The valve housing contains no user replaceable items.
Technical specifications
Operating data
Max Flow rate 100 ml/min
Max Pressure 25 MPa (250 bar, 3600 psi)
Back pressure <50 kPa at 100 ml/min with water
Leakage <0.1 μl/min at 25 MPa
pH stability range 1–13, 1–14 (<1 day exposure)
Viscosity Max. 5 cP
Switch time <260 ms between two adjacent positions
Operating life time >50 000 cycles, two adjacent positions
Environment +4 to +40 °C
20–95% relative humidity
84–106 kPa (840–1060 mbar)
atmospheric pressure
6 Reference information
3
INV-907
Housing
Valve body

20 Valve INV-907 Instructions 56-8101-01 Edition AE
6 Reference information
Physical data
Internal volume
Pos 1 LOAD
Port 1–7 9 μl
Port 2–3 5 μl
Port 4–6 8 μl
Pos 2 INJECT
Port 1–2 7 μl
Port 3–5 6 μl
Port 6–7 9 μl
Pos 3 WASH
Port 2–4 8 μl
Port 5–7 9 μl
Flow channel diameter 0.8 mm
Valve principle Motor controlled valve
Functions Switching 3 positions controlled
from UNICORN
Degree of protection IP 43
Wetted materials PEEK (polyetheretherketone)
Chemical resistance The wetted parts are resistant to organic
solvents and salt buffers commonly used
in chromatography of biomolecules, except
100% ethylacetate, 100% hexane and
100% tetrahydrofuran (THF)
Power requirement 32 V DC ±10% from the system pump
Power consumption Up to 9 W
UniNet 2 address 0–9
Inlet and outlet tubing UNF 10–32 2B ”Fingertights” for capillary
tubing 1/16” outer diameter
Dimensions,
H × W × D 135 × 80 × 120 mm
Weight 1.2 kg
EMC Standards This device meets the requirement of the
EMC Directive 89/336/EEC through the
following harmonized standards:
• EN 61326 (emission and immunity)
• EN 55011, GR 2, Class A (emission)
• This device complies with part 15 of
the FCC rules (emission). Operation is
subject to the following two conditions:
1 The device may not cause harmful
interference.
2 This device must accept any
interference recieved, including
interference that may cause
undesired operation.