3
• Molecules larger than the largest pores in the Sephadex matrix are
excluded from the matrix and are eluted first, in or just after the
void volume. The void volume is the column volume outside the
Sephadex matrix.
• Molecules smaller than the largest pores in the Sephadex matrix
will penetrate the pores to varying extent. They have a larger
accessible column volume than the large molecules and therefore
they elute after the large molecules just before one total column
volume of buffer has passed through the column.
Group separation can be made using two different protocols, gravity
protocol and spin protocol.
GE provides an assortment of sample clean-up products. The
different formats available are summarized in Table 1.
Table 1. Product overview
Clean Up
product
Exclusion
limit, Mr
Bed
volume
Sample volume
gravity protocol1
Sample colume
spin protocol1
PD SpinTrap™
G-25
5000 0.5 mL - 100 to 180 µL
PD MultiTrap™
G-25
5000 0.5 mL - 70 to 130 µL
PD MiniTrap™
G-25
5000 2.1 mL 0.1 to 0.5 mL 0.2 to 0.5 mL
PD MidiTRap™
G-25
5000 3.5 mL 0.5 to 1.0 mL 0.75 to 1.0 mL
PD-10 Desalting
Columns
5000 8.3 mL 1.0 to 2.5 mL 1.75 to 2.5 mL
PD MiniTrap
G-10
700 2.1 mL 0.1 to 0.3 mL -
PD MidiTrap
G-10
700 5.3 mL 0.4 to 1.0 mL -
1Recommended sample volumes.
