Gilson ASPEC GX-271 User manual

Gilson,Inc. | PO Box 620027 | Middleton, WI 53562-0027 | Tel: [phone] OR 800-445-7661 | Fax: [phone]
GilsonS.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 Villiers-le-bel, FRANCE
www.gilson.com | [e-mail] | ser[email protected] | [email protected]
GX-271 Liquid Handler
User’s Guide
LT350012-04

3
GX-271 Liquid Handler | User’s Guide
Table of Contents
Safety
Electronic and Hazard Symbols.....................................................10
Safety Notices .....................................................................11
Lifting.............................................................................11
Voltage............................................................................11
Probes ............................................................................11
Solvents...........................................................................11
Replacement Parts.................................................................11
Sécurité
Symboles Électroniques et de Dangers .............................................14
Notes de Sécurité..................................................................15
Déplacement......................................................................15
Tension............................................................................15
Aiguilles...........................................................................15
Solvants...........................................................................15
Pièces Détachées ..................................................................15
1 Introduction
Description........................................................................18
GX-271 Liquid Handler with GX Solvent System .................................18
GX-271 Liquid Handler with VERITY® Syringe Pump .............................19
Unpacking ........................................................................20
Standard Equipment...........................................................21
Accessories ....................................................................22
Customer Service ..................................................................23
Trademarks........................................................................23
Technical Specications............................................................24

Table of Contents GX-271 Liquid Handler
|
Table of Contents
2 Installation
Locator Pan Installation and Setup .................................................28
Locator Pan Installation ........................................................29
Rinse Station Setup ............................................................29
Rinse Station Locations ........................................................30
Rinse Station Installation on the Tray Insert .....................................31
Tray Insert Installation .........................................................32
Z-Arm Setup.......................................................................33
Isolator Probe Holder Installation...............................................34
Guide Foot Installation and Setup ..............................................35
Z-Arm Installation..............................................................36
Adjusting the Z Travel Height...................................................37
Z-Arm Height Adjustment......................................................38
Probe Installation ..............................................................39
Liquid Level Detection (LLD) Cable Installation..................................39
Plumbing Connections ............................................................40
Transfer Tubing Installation ....................................................40
Rinse Station Plumbing ........................................................41
Rear Panel Connections............................................................42
Rear Panel Diagram ............................................................42
Fuses..........................................................................42
Ethernet.......................................................................43
RS-232 ........................................................................44
GSIOC .........................................................................45
Z-Arm Connection .............................................................49
Input and Output Ports ........................................................49
Power .........................................................................50
Rack Installation ...................................................................51
Code 33X/34X-Series Rack Installation ..........................................51
Solvent Bottle Rack Installation.................................................51

GX-271 Liquid Handler User’s Guide
|
Table of Contents
Final Z-Arm Adjustments...........................................................52
Z-Arm Cable Support Rod Installation ..........................................52
Spiral Wrap Installation ........................................................52
Z-Arm Movement Verication ..................................................52
3 Operation
Front Panel ........................................................................54
Power Indicator Light ..........................................................54
Error Indicator Light ...........................................................54
Start Up ...........................................................................54
GX-27XSeriesOsetUtility ........................................................55
Install the GX-27X Series Oset Utility ..........................................55
Prepare to Run the GX-27X Series Oset Utility..................................56
Start the GX-27X Series Oset Utility............................................56
Use the GX-27X Series Oset Utility.............................................57
4 Maintenance
Helpful Hints ......................................................................62
Cleaning ..........................................................................62
Liquid Handler.................................................................62
Fluid Path .....................................................................62
Part Replacement..................................................................64
Tubing ........................................................................64
Probe .........................................................................64
Fuse...........................................................................66
Transporting the Instrument .......................................................66

Table of Contents GX-271 Liquid Handler
|
Table of Contents
5 Troubleshooting
Error Messages ....................................................................68
Mechanical Troubleshooting .......................................................71
Probe No Longer Finding Tube Center ..........................................71
Electrical Troubleshooting .........................................................71
Input Functions Not Operating .................................................71
Output Functions Not Operating ...............................................71
Unit Not Operational...........................................................71
Unit Blows Fuses...............................................................71
Communication Troubleshooting ..................................................72
Repair and Return Policies .........................................................72
Before Calling Us...............................................................72
Warranty Repair ...............................................................72
Non-Warranty Repair...........................................................72
Return Procedure ..............................................................73
Unit End of Life ................................................................73
A GX Solvent System
Technical Specications............................................................76
Installation ........................................................................77
Operation .........................................................................78
Start Up .......................................................................78
Aspirating Fluid from a Well ....................................................78
Dispensing Fluid to a Well......................................................78
Plumbing Connections ............................................................79
Connection Diagrams..........................................................80
Port Connections and Plumbing................................................81
Rear Panel Connections............................................................82
Unit ID ........................................................................83

GX-271 Liquid Handler User’s Guide
|
Table of Contents
B Fraction Collection Valve
Technical Specications............................................................86
Installation ........................................................................87
Valve and Power Cable.........................................................87
Tube and Wire Routing Strip....................................................87
Plumbing Connections ............................................................88
Assembling the Collection Probes ..............................................88
Installing the Collection Probes ................................................89
Valve Connections and Description.............................................90
C GX Direct Injection Module
Technical Specications............................................................92
Installation ........................................................................94
Injection Module ..............................................................94
Injection Module with Riser Block ..............................................94
Plumbing Connections ............................................................95
Port Connections and Tubing ..................................................96
Rear Panel Connections............................................................97
Unit ID ........................................................................98
D GX Rinse Pump
Technical Specications........................................................... 100
Installation ....................................................................... 101
Plumbing Connections ........................................................... 102
Rear Panel Connections........................................................... 103
E Safety Shield
Installation ....................................................................... 106

Table of Contents GX-271 Liquid Handler
|
Table of Contents
F Parts and Accessories
GX-271 Liquid Handler............................................................ 107
Systems ...................................................................... 107
Probes........................................................................ 107
Probe Guide Assemblies ...................................................... 110
Z-Arm and Components ...................................................... 110
Rinse Stations ................................................................ 111
Cables and I/O Accessories .................................................... 111
Miscellaneous ................................................................ 111
GX Solvent System................................................................ 112
Transfer Tubing Packages ..................................................... 112
Transfer Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Inlet Tubing Assembly ........................................................ 112
Accessories ................................................................... 112
GX Rinse Pump ................................................................... 113
Components ................................................................. 113
GX Direct Injection Modules ...................................................... 113
Sample Loops ................................................................ 113
Injection Ports ................................................................ 115
Accessories ................................................................... 115
Fraction Collection Valve.......................................................... 116
Components ................................................................. 116
Racks......................................................................... 117
Rack Heighteners ............................................................. 121
Tray Insert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
G Materials
Liquid Contact Materials .......................................................... 123

9
GX-271 Liquid Handler | User’s Guide
Safety
Read this chapter before installing and operating the instrument.
Only trained technical personnel in a laboratory environment may use the instrument for non-medical, liquid
handling purposes. For safe and correct use of the instrument, operating and service personnel must follow
all instructions contained in this guide when installing, cleaning, and maintaining the instrument. All safety
precautions must be observed during all phases of operation, service, and repair of the instrument.
Failure to comply with these precautions or with warnings described in the user’s guide violates safety
standards of design, manufacture, and intended use of the instrument. Gilson assumes no liability for
customers failing to comply with these requirements.
The instrument has been certied to safety standards required in Canada, Europe, and the United States.
Refer to the rear panel label on the instrument and the Declaration of Conformity document for the current
standards to which the instrument has been found compliant.

10
Safety
Safety |GX-271 Liquid Handler
Electronic and Hazard Symbols
The following electronic and hazard symbols may appear on the instrument:
Symbol Explanation
~
Alternating current
Courant alternatif
Wechselstrom
~
Direct current
Courant continu
Gleichstrom
Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
|
Electrical power ON
Sous tension
Netzschalter ein
O
Electrical power OFF
Hors tension
Netzschalter aus
Caution
Attention
Vorsicht
Caution, risk of electric shock
Attention, risque de choc électrique
Vorsicht, Elektroschockgefahr
Caution, hot surface
Attention, surface chaude
Vorsicht, heiße Oberäche
Fuse
Fusible
Sicherung
Keep hands clear of probe
Garder les mains éloignees de l’aiguille
Halten Sie Hände fein von der Nadel

11
Safety
GX-271 Liquid Handler | User’s Guide
Safety Notices
The following safety notices may appear in this document:
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, may result
in serious injury
CAUTION
CAUTION indicates a potentially hazardous situation which, if not avoided, may result
in minor or moderate injury
NOTICE
NOTICE indicates a potentially hazardous situation which, if not avoided, may result in
equipment damage
Lifting
The instrument exceeds the weight one person can lift safely. Two or more people are required to lift the
instrument safely. Always lift the instrument from the base and follow any unpacking instructions provided
with the instrument.
Voltage
Ensure that the rear panel is easily accessible. Detach all sources of voltage from the instrument before the
service, repair, or exchange of parts. Use only the grounded AC cord provided. Ungrounded power cords
can result in electrical shock and serious personal injury. Use only approved fuses with the specied current
rating. The instrument must be operated within the voltage specied on the rear panel of the instrument.
Probes
Keep clear of the probe while the X/Y/Z arm is in motion to avoid personal injury. Probes may contain
hazardous substances.
Solvents
Observe safe laboratory practices when handling solvents. Adequate safety precautions, such as proper
ventilation, safety glasses, etc., must be used when handling dangerous liquids. Refer to the Material Safety
Data Sheet (MSDS) for each solvent before use.
Replacement Parts
Only use the replacement parts mentioned in this user’s guide.

13
GX-271 Liquid Handler | User’s Guide
Sécurité
Merci de lire attentivement cette section avant toute installation ou utilisation de l’instrument.
Cet instrument est exclusivement destiné à être utilisé dans un environnement de laboratoire, par un
personnel qualié, à des ns de manipulations de liquides non-médicales. Pour une utilisation correcte
et en toute sécurité de l’instrument, il est nécessaire que le personnel qui utilise et réalise la maintenance
de l’instrument, suive les instructions contenues dans ce guide lors de l’installation, du nettoyage et de la
maintenance de l’instrument. Toutes les consignes de sécurité doivent être respectées durant toutes les
phases de fonctionnement, d’entretien ou de réparation de l’instrument.
Le non-respect de ces précautions ou des avertissements spéciques mentionnés dans ce guide compromet
les normes de sécurité de conception, de fabrication et d’utilisation prévue de l’instrument. Gilson décline
toute responsabilité en cas d’incapacité du client à se conformer à ces exigences.
L’instrument a été certié conformément aux normes de sécurité en vigueur au Canada, en Europe et aux
Etats-Unis. Merci de vous reporter aux indications mentionnées sur le panneau arrière de l’instrument
ainsi qu’au document de Déclaration de Conformité aux normes pour lesquelles l’instrument a été déclaré
conforme.

14
Sécurité
Sécurité |GX-271 Liquid Handler
Symboles Électroniques et de Dangers
Les symboles électroniques et de dangers suivants peuvent apparaitre sur l’instrument:
Symbole Signication
~
Alternating current
Courant alternatif
Wechselstrom
~
Direct current
Courant continu
Gleichstrom
Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
|
Electrical power ON
Sous tension
Netzschalter ein
O
Electrical power OFF
Hors tension
Netzschalter aus
Caution
Attention
Vorsicht
Caution, risk of electric shock
Attention, risque de choc électrique
Vorsicht, Elektroschockgefahr
Caution, hot surface
Attention, surface chaude
Vorsicht, heiße Oberäche
Fuse
Fusible
Sicherung
Keep hands clear of probe
Garder les mains éloignees de l’aiguille
Halten Sie Hände fein von der Nadel

15
Sécurité
GX-271 Liquid Handler | User’s Guide
Notes de Sécurité
Les notes de sécurité suivantes peuvent apparaitre dans ce document:
WARNING
WARNING (AVERTISSEMENT) indique une situation potentiellement dangereuse qui, si
elle n’est pas évitée, peut entraîner des blessures graves.
CAUTION
CAUTION (ATTENTION) indique une situation potentiellement dangereuse qui, si elle
n’est pas évitée, peut entraîner des blessures mineures ou légères.
NOTICE
NOTICE (AVIS) indique une situation potentiellement dangereuse qui, si elle n’est pas
évitée, peut entraîner des dommages matériels.
Déplacement
Le poids de l’instrument implique que deux personnes ou plus sont requises pour le déplacer en toute
sécurité. Toujours soulever l’instrument par sa base et suivre les instructions de déballage fournies avec
l’appareil.
Tension
S’assurer que l’accès au panneau arrière est libre. Déconnecter la source d’alimentation avant toute
opération d’entretien, de réparation ou de remplacement de pièces. Utiliser exclusivement le bloc et le
cordon d’alimentation avec raccordement à la terre fournis. Un cordon d’alimentation sans terre peut
provoquer choc électrique et graves blessures. Utiliser exclusivement des fusibles de l’intensité et du type
spécié. Pour le fonctionnement, respecter la tension indiquée sur le panneau arrière de l’instrument.
Aiguilles
An d’éviter tout risque de blessure, rester à distance des aiguilles lorsque le bras X/Y/Z est en mouvement.
Les aiguilles peuvent contenir des substances dangereuses.
Solvants
Respecter les Bonnes Pratiques de Laboratoire lors de la manipulation de solvants. Si des liquides dangereux
sont utilisés, s’assurer que la ventilation est adéquate et porter en permanence un équipement de protection
individuelle (EPI), tel que : lunettes, gants et vêtements de protection. Se reporter aux Fiches de Données de
Sécurité relatives aux solvants avant toute utilisation.
Pièces Détachées
S’assurer d’utiliser seulement les pièces détachées mentionnées dans le guide utilisateur. S’il est nécessaire
de changer des pièces non listées, merci de contacter votre représentant Gilson local.

17
GX-271 Liquid Handler | User’s Guide
1Introduction
Chapter One
This chapter provides information on the following topics:
●Description on page 18
●Unpacking on page 20
●Customer Service on page 23
●Trademarks on page 23
●Technical Specications on page 24

18
Description
Introduction |GX-271 Liquid Handler
Description
The GX-271 Liquid Handler is an X/Y/Z instrument that automates liquid handling procedures. The
liquid handler can be congured with a GX Solvent System or VERITY® Syringe Pump, rinse stations, and
racks. Optional accessories include a GX Direct Injection Module, GX Rinse Pump, and fraction collection
valve.
GX-271 Liquid Handler with GX Solvent System
Locator
Plate
GX Direct
Injection Module
Mounted on the
locator plate of the
GX-271 Liquid Handler.
The injection module
has an option of four
dierent valves (for
1/8"OD sample loops or
1/16" OD sample loops).
The valves available for
the injection module
feature a vertical
direct connection for
the injection port.
One injection module
can be placed on the
locator plate of the
GX-271 Liquid Handler.
For plumbing
instructions, refer
to page 95 of
its appendix.
GX Solvent System
A bi-directional pump that can switch
from aspirate to dispense mode.
The solvent system can handle ow
rates up to 50 mL/min. The solvent
selection valve can accommodate up
to ve dierent reservoir solvents.
For plumbing instructions, refer
to page 79 of its appendix.
Tray Insert
Figure 1: System Overview and Description of the GX-271 Liquid Handler with GX Solvent System
Fraction Collection Valve
A three-way valve
mounted low on the
Z-arm. This design
allows for reduced dead
volume and collection
of larger volumes.
For plumbing instructions,
refer to page 88 of
its appendix.

19
Description
GX-271 Liquid Handler | User’s Guide
GX-271 Liquid Handler with VERITY® Syringe Pump
VERITY® Syringe Pump
A syringe pump that can
be used to deliver up to
25 mL in one stroke and
down to 10 μL (typical
value) with high accuracy.
For more information,
refer to its user’s guide.
Figure 2: System Overview and Description of the GX-271 Liquid Handler with VERITY® Syringe Pump
GX Rinse Pump
Integration of a GX Rinse Pump
provides the option for a owing rinse
using high or low ow settings.
For installation instructions, refer to
page 101 of its appendix.
Rinse Stations
Probe rinsing occurs
through up to three
dedicated rinse stations
capable of performing
inside and outside
rinses, and a owing
outside rinse when
the system includes
a GX Rinse Pump.
For plumbing
instructions, refer
to page 41.
Z Arm
For installation
instructions,
refer to
page 33.
Locator Pan
For installation
instructions, refer
to page 28.
Racks
For installation
instructions, refer
to page 51.
Safety Shield
For installation
instructions, refer
to page 106 of
its appendix.
Cable
Support
Rod

20
Unpacking
Introduction |GX-271 Liquid Handler
Unpacking
The instrument is delivered with most major components already assembled. Keep the original container
and packing assembly in case the instrument must be returned to the factory.
To unpack the instrument:
1. Open the box and remove the foam inserts.
2. Remove the accessory box from the locator plate of the instrument and set aside.
3. Grip the instrument at the base and lift it out of the box. Place it on a lab bench or cart.
It is recommended that two people lift the unit out of the box.
Do not attempt to lift the instrument from the Y-arm (the horizontal arm).
4. Remove the plastic wrap covering the liquid handler.
Figure 3: Unpacking the GX-271 Liquid Handler (Bottom View)
Lift Here