Gilson MICROMAN E M10E User manual

Ergonomic Pipetting for Problem Liquids!

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1  INTRODUCTION
Congratulations on purchasing your new MICROMAN® E
pipette.MICROMANEissuitableformanydierenttypes
ofapplication,includingpipettingvolatile,viscous,dense,
orhighsurfacetensionliquids.It isaquality productfrom
Gilson, which is fully compliant ISO 8655, CE labeled.
The six models available allow for precise and accurate
pipetting of viscous, dense and volatile liquids.They also
enable contamination-free pipetting, avoiding vapor
contamination and cross-contamination. M25E, M50E
and M250E, with thin and long CPs are especially suited
for use with long or narrow vessels.
Enjoy the following features:
- MICROMAN E pipettes are equipped with a positive
displacement mechanism isolating the aspirated
liquid from the pipette body. The positive displace-
ment mechanism prevents the sample-to-sample
contamination that can result from the aerosol eect.
- MICROMAN E uses disposable capillaries and pis-
tons. These are the only parts to be in touch with the
aspiratedliquids,andreplacementthereofwillprovide
absolute protection against contamination caused
by carryover between samples or reagents. Simply
replace the capillary and piston between assays; they
are ejected automatically and simultaneously, thus
avoiding any risk for the operator.
- MICROMAN E is equipped with a direct reading
volumeter that allows precise adjustment of the
required volume. MICROMAN E is permanently
calibrated.
At the manufacturing stage, the capillary and piston
are positioned automatically, and therefore require
no further calibration.
- MICROMANErequiresnolubricationormaintenance,
due to the nature and quality of the materials used in
its component parts.
Contents Page
1 - INTRODUCTION 3
2 - PARTS CHECKLIST 4
3 - DESCRIPTION 4
4 - OPERATING RANGES AND MATERIALS 5
5 - FITTINGTHE CAPILLARY AND PISTON 5
6 - SETTING THE VOLUME 7
7 - PIPETTING 8
8 - GLP FEATURES 9
9 - TROUBLESHOOTING 9
10 - CLEANING AND DECONTAMINATION 10
11 - SPECIFICATIONS 12
WARRANTY 14
EC DECLARATION OF CONFORMITY 15
Before use, please carefully read Warranty
page 14 of this User's Guide!

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2  PARTS CHECK LIST
Just take a moment to verify that the following items
are present:
MICROMAN E,
User's Guide,
Safety bag,
Adhesive id-tags (strip of 6),
Capillary pistons (10),
Certicate of conformity
(including barcode sticker).
3  DESCRIPTION
1Color-coded push-button and
magnier with volume range
for aspirating and dispensing.
2Body or handle.
3Capillary.
Piston.
Shaft.
Thumbwheel for
setting the vol-
ume.
Volumeter.
 Volume unlock button.
 Data matrix.






Never lubricate the capillary holder, capillary,
or any other part of the pipette. If the capillary
tends to slip off the capillary holder, clean the
capillary holder with ethanol, using a medical
wipe or similar soft tissue.
CP Capillary Piston
(C) (P)
CP10 Polypropylene Polyester
CP25 Polypropylene Polyester
CP50 Polypropylene Polyester
CP100 Polypropylene Polyethylene
CP250 Polypropylene Polyethylene
CP1000 Polypropylene Polyacetal
5  FITTING THE CAPILLARY AND PISTON


4  OPERATING RANGES AND MATERIAL
Available Models
Model Volume Range
M10E 1 µL to 10 µL
M25E 3 µL to 25 µL
M50E 20 µL to 50 µL
M100E 10 µL to 100 µL
M250E 50 µL to 250 µL
M1000E 100 µL to 1000 µL
Materials
Model Spring Clamp Shaft Body
(B)
M10E SS Be Alloy PBT PVDF/PP
M25E SS Be Alloy PVDF PVDF/PP
M50E SS Be Alloy PVDF PVDF/PP
M100E SS Be Alloy PBT PVDF/PP
M250E SS Be Alloy PVDF PVDF/PP
M1000E SS Be Alloy PVDF PVDF/PP
Abbreviations
SS = Stainless Steel
Be = Beryllium
PBT=PolybutyleneTerephthalate
PP = Polypropylene
PVDF = Polyvinylidene Fluoride
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Make sure the capillary piston (CP)
model matches the MICROMAN E
you are about to use:
CP25, CP50 and CP250 Bulk
The capillaries and pistons are deliv-
ered in two separate boxes. To t a
capillary piston,proceedasillustrated:
1) Take care not todamage the
sealingtipwhenhandling
the piston .
2) Select a piston and slide
it into the capillary
 .
3) Gently push the capillary
until it snaps onto the capil-
lary holder.
4) Slowly press the push-
button to the rst stop to
attach the stem . You
should denitely feel and
hear a slight click.
button to the rst stop until you feel and hear a slight
click
3 .
4) Do not release the push-button and pipette your
solution.
6  SETTING THE VOLUME
The volume of liquid to be aspirated is set using the
volumeter. The volumeter consists of three number dials,
which are read from top (most signicant digit) to bottom
(least signicant digit). A marker is used to set exact or
intermediate volumes using the scale on the bottom dial.
The dials are colored either black or red to indicate the
position of the decimal point, according to the model (see
examples).
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

MICROMAN E
CP model
CP10 M10E
CP25 M25E
CP50 M50E
CP100 M100E
CP250 M250E
CP1000 M1000E
CP10, CP25, CP50, CP100,
CP250 and CP1000Tipack
Capillary pistons (CP) are
deliveredreadytouse.To t
a capillary piston, proceed
as follows:
1)Gently press the
MICROMAN E onto the
capillary piston until it
is rmly seated on the
MICROMAN E
 .
2) Pickupthecapillaryfrom
the rack
2 .
3) Slowly press the push-
Model Color of volumeter numbers
Black Red
Increment
M10E, M25E, M50E µL 0.1 µL 0.02 µL
M100E, M250E µL none 0.2 µL
M1000E 0.1 and 0.01 mL mL 2 µL

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The volume is set by turning the thumb-
wheelslowlytoreachtherequiredsetting.
For small volume changes, directly and
slowly turn the thumbwhell.
To obtain maximum accuracy when
setting the volume, proceed as follows:
- when decreasing the volume setting,
slowlyreachtherequiredsetting,mak-
ing sure not to overshoot the mark.
8
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8
36.8 µL
M50
0
6
6.8 µL
M25
1
6
8
168 µL
M250
0
7
5
0.75 mL
M1000
E E
E E
0
6
8
6.8 µL
M10
0
6
8
68 µL
M100
E
E
0
6
8
6.8 µL
M10
0
6
8
68 µL
M
100
E
E
8
3
6
8
36.8 µL
M50
0
6
6.8 µL
M25
1
6
8
168 µL
M
250
0
7
5
0.75 mL
M1000
E E
E E

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- whenincreasingthe volumesetting,passtherequired
value by 1/3 of a turn and then slowly decrease the
volume to reach the required setting, making sure not
to overshoot the mark.
Forlargevolumechanges,proceed
as follows :
1) Push the unlock button counter.
2) When decreasing the volume
setting, approach the required
setting, by 1/3 of a turn.
When increasing the volume
setting, pass the required setting
by 1/3 of a turn.
3)
Release the unlock button counter.
7  PIPETTING
Aspirating
- Press the push-button to the
rst stop (1),
- Immerse the capillary 2 mm
into the liquid,
- Slowly release the push-
button to draw up the liquid
(top position),
- Wipe any liquid from the out-
sideofthecapillary,takingcare
not to touch the orice.
Dispensing
- Place the end of the capillary against the inside wall
of the recipient vessel,
- Press the push-button slowly to the rst stop,
- Keeping the push-button depressed, move the capil-
lary away from the sidewall,
- Withdraw MICROMAN E from the vessel and release
the push-button.
Ejecting the capillary piston
Press the push-button to the rst stop (1), then press
down harder to the second stop (2); at this point the
piston and capillary are ejected simultaneously.
MICROMAN E is automatically calibrated by correctly fitting a
capillary piston. Once the volume is set, measurements will be
accurate and precise.
8  GLP FEATURES
- No need to touch disposables (capillary piston).
- Serial Number: engraved on pipette body.
- Barcode: on the box and with the certicate (can be
transferred).
- ID Tag (Application or User).
- Color-coded push-button.
- Color-codedmagnierwithvolumerange.
- Data matrix engraved on pipette body.
- Gilson certicate of conformity according
to ISO 8655.
9  TROUBLESHOOTING
You may be able to identify and correct the problem
by referring to the following table. If you cannot solve
the problem, please contact your Gilson representative.
Leaks:
Replace the capillary and piston.
No stroke:
The piston is not properly tted into the clamp; check
that the piston is seated correctly (refer to pages 5-7).
Without any capillary piston, press the push-button to
the rst stop, then press down harder to the second stop.
Dicult to t a capillary:
Clean the capillary holder with ethanol. Without any
Top
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capillary piston, press the push-button to the rst stop,
then press down harder to the second stop.
Inaccuracy:
Make sure the capillary is correctly mounted on the
capillary holder (refer to pages 5-7).
Imprecision:
Replace the capillary and piston.
Dicult to set the volume:
As this suggests internal damage to the pipette you
should contact your Gilson representative.
If the problem persists...
Contact your Gilson representative.
10  CLEANING AND DECONTAMINATION
MICROMAN E is designed so that parts normally in
contact with liquid contaminants are easy to clean and
decontaminate.
Cleaning
Clean the pipette with soap solution prior to decon-
tamination.
External
1) Wipe the entire pipette with a soft cloth or lint-free
tissue impregnated with soap solution to remove any
stains. If the pipette is very dirty, a brush with soft
plastic bristles may be used.
2) Wipe the entire pipette with a soft cloth or lint-free
tissue impregnated with distilled water.
3) Leave the parts to dry by evaporation or wipe them
down with a clean soft cloth or lint-free tissue.
Internal
Only the following components should be immersed in
adecontaminantsolution:clampassembly,returnspring
and capillary holder (shaft).
1) Unscrew the capillary holder (shaft).
2) Remove the clamp assembly and return spring from
the shaft.
3)
Set aside the body (handle) in a dry and secure location.
4) Clean the individual components using an ultrasonic
bath (for 20 minutes at 50 °C) or with a soft cloth and
brush. Small round brushes with soft plastic bristles
may be used to clean the interior of the shaft.
5) Rinse the individual components with distilled water.
6) Leave the parts to dry by evaporation or wipe them
down with a clean soft cloth or lint-free tissue.
7) Reassemble the pipette.
Decontamination
You may refer to the Decontamination Procedure
LT802288 available at www.gilson.com.
Chemical Decontamination
Clean the pipette prior to decontamination. Full details
ofrecommendeddecontaminationproceduresforGilson
pipettesareavailablefromyoursupplier.Whicheverother
decontaminant you use, check with the supplier that the
product is compatible with the materials used for the
componentparts of the pipette,andthatdoes notattack
either of the following plastics and metals: polyester,
PVDF (polyvinylidene uoride), PP (polypropylene), PBT
(polybutylene terephthalate), PC (polycarbonate), SS
(stainless steel), and BE Alloy (beryllium).
Non-immersible Parts
1) Wipe the body (handle) of the pipette with a soft
cloth or lint-free tissue impregnated with the chosen
decontaminant.
2) Wipe the body (handle) of the pipette with a soft cloth
or lint-free tissue impregnated with distilled water or
sterilized water.
Before returning any pipette, ensure that it is com-
pletely free of chemical, biological, or radioactive
contamination. Use the safety bag provided by
Gilson.

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Immersible Parts
Only the following components should be immersed in
adecontaminantsolution:clampassembly,returnspring
and capillary holder (shaft).
1) Unscrew the capillary holder (shaft).
2) Remove the clamp assembly and return spring from
the shaft.
3)
Set aside the body (handle) in a dry and secure location.
4) Immerse the components in the decontaminant
solution or wipe them down according to the
instructions providedbythe manufacturerorsupplier
of the decontaminant.
5) Rinse the individual components with distilled or
sterilized water.
6) Leave the parts to dry by evaporation or wipe them
with a clean lint-free tissue or a soft cloth.
7) Reassemble the pipette.
11  SPECIFICATIONS
MICROMAN E is a high quality pipette that oers excel-
lent accuracy and precision. The gures provided in the
“GilsonMaximum PermissibleErrors”tablewereobtained
using
GilsonCPs.Theseguresareonlyguaranteedwhen
using genuine Gilson CPs.
Each pipette is inspected and validated by qualied
technicians according to the Gilson Quality System.
Gilson declares that its manufactured pipettes comply
with the requirements of the ISO 8655 Standard, by
type testing.The adjustment is carried out under strictly
dened and monitored conditions (ISO 8655-6):
• Basis of adjustment, Ex.
• Reference temperature, 20 °C
• Relative humidity, 50%
• Barometric pressure, 101 kPa
• Use of distilled water grade 3 (ISO 3696)
• Ten measurements for each test volume, which are
nominal volume, 50% of nominal volume, and the
minimum or 10% of nominal volume.
Performance Tests
Each pipette is inspected and validated according to the
Gilson Quality Assurance System. Based on extensive
historical data, manufacturing conditions and expertise,
andincompliancewithISOstandardsrelativetostatistical
process control, the assurance level of this instrument
performing to specications is 99.8%. Specications rely
onqualityandconsistencyforthewholepipettingsystem
and are guaranteed only when the pipette is used with
Gilson capillary pistons.
Gilson Maximum Permissible Errors
The data given in the table conform to the ISO 8655-2 Standard.
Maximum Permissible Errors
Model Volume Gilson ISO 8655
(Reference) (µL) Systematic Random Systematic Random
error (µL) error (µL) error (µL) error (µL)
M10E Min 1 ± 0.09 0.03 ± 0.2 0.1
(FD10001) 5 ± 0.10 0.03 ± 0.2 0.1
Max. 10 ± 0.15 0.06 ± 0.2 0.1
M25E Min. 3 ± 0.25 0.08 ± 0.7 0.3
(FD10002) 10 ± 0.27 0.08 ± 0.7 0.3
Max 25 ± 0.30 0.10 ± 0.7 0.3
M50E Min. 20 ± 0.34 0.20 ± 0.7 0.3
(FD10003) Max. 50 ± 0.70 0.30 ± 0.7 0.3
M100E Min. 10 ± 0.50 0.20 ± 1.5 0.6
(FD10004) 50 ± 0.75 0.30 ± 1.5 0.6
Max. 100 ± 1.00 0.40 ± 1.5 0.6
M250E Min. 50 ± 1.50 0.30 ± 6 2.0
(FD10005) 100 ± 1.70 0.30 ± 6 2.0
Max. 250 ± 2.50 0.50 ± 6 2.0
M1000E Min. 100 ± 3.0 1.6 ± 12 4.0
(FD10006) 500 ± 5.0 2.5 ± 12 4.0
Max. 1000 ± 8.0 4.0 ± 12 4.0

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WARRANTY
Gilson warrants this pipette against defects in material under normal
use and service for a period of 12 months from the date of purchase.
This warranty shall not apply to pipettes which are subject to abnormal
use and/or improper or inadequate maintenance (contrary to the
recommendations given in the User’s guide), including, but not limited
to pipettes which have been subjected to physical damage, improper
handling, spillage or exposure to any corrosive environment. This war-
ranty shall also be void in the event pipettes are altered or modified
by any party other than Gilson or its designates. Gilson’s sole liability
under this warranty shall be limited to, at Gilson’s sole option, repair or
replacement of any defective components of pipettes or refund of the
purchase price paid for such pipettes.
THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY
DISCLAIMSALLOTHERWARRANTIES,WHETHER EXPRESSOR IMPLIED,
INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY
WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO
CIRCUMSTANCES SHALLGILSON BELIABLE FORANYCONSEQUENTIAL,
PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY
BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.
EC DECLARATION OF CONFORMITY
MICROMAN® E
M10E, M25E, M50E, M100E, M250E, M1000E
Positive displacement Capillaries/Pistons
98/79/EC*
on In Vitro Diagnostic Medical Devices
comply with the requirements of the following European
Directives:
Villiers-le-Bel, June 20th, 2014
E. Chamault H. Le Dorze
General Manager Quality Manager
GILSON S.A.S.
19, avenue des Entrepreneurs
BP. 145 - 95400 Villiers-le-Bel (France)
Tel.: [phone]
Fax: [phone]
www.gilson.com
* Annex III, self-declared
The company,
Hereby certifies under its sole responsibility that the products
listed below:

LT801547/A - ©2014 Gilson SAS All rights reserved June 2014
English Printed in France
Specifications subject to change without notifications - errors omitted.
Gilson, Inc. World Headquarters
3000 Parmenter Street I P.O. Box 620027 I Middleton, WI 53562-0027, USA
Tel: [phone] or (1) 608-836-1551 I Fax: [phone]
Gilson S.A.S.
19, avenue des Entrepreneurs I BP 145, F-95400 Villiers-le-Bel, France
Tel: [phone] I Fax: [phone]
www.gilson.com
[email protected]
[email protected]
[email protected]