Gilson Pipetman P2G User manual

Gilson, Inc. World Headquarters I3000 Parmenter Street I P.O. Box 620027
Middleton, WI 53562-0027 USA
Tel: [phone] or (1) 608-836-1551 IFax: (1) 608-831-4451
Gilson S.A.S.I19, avenue des Entrepreneurs
IBP 145, F-95400 Villiers-le-Bel, FRANCE
Tel: [phone] IFax: (33-1) 34 29 50 20
LT800511/C © 05/2012 Gilson SAS.
All rights reserved. All trademarks
are the property of Gilson Inc. and
its subsidiaries.
www.gilson.com I[email protected] Iser[email protected] Itr[email protected]
Premium pipetting comfort!
EXTREMELY LOW PIPETTING FORCES!
EXTREMELY EASY TIP EJECTION!
LEGENDARY PIPETMAN ROBUSTNESS!
ADJUSTABLEVOLUME PIPETTE
Trusted results
Best results guaranteed with a performance exceeding ISO recommendations.
Model Volume
tested
Maximum Permissible Errors
Gilson ISO 8655
Systematic error
(μL)
Random error
(μL)
Systematic error
(μL)
Random error
(μL)
P2G
(F144054P)
(F144054M)
Min.: 0.2
0.5
Max.: 2
± 0.024
± 0.025
± 0.030
≤ 0.012
≤ 0.012
≤ 0.014
± 0.08
± 0.08
± 0.08
≤ 0.04
≤ 0.04
≤ 0.04
P10G
(F144055P)
(F144055M)
Min.: 1
5
Max.: 10
± 0.025
± 0.075
± 0.100
≤ 0.012
≤ 0.030
≤ 0.040
± 0.12
± 0.12
± 0.12
≤ 0.08
≤ 0.08
≤ 0.08
P20G
(F144056P)
(F144056M)
Min.: 2
10
Max.: 20
± 0.10
± 0.10
± 0.20
≤ 0.030
≤ 0.050
≤ 0.060
± 0.2
± 0.2
± 0.2
≤ 0.1
≤ 0.1
≤ 0.1
P100G
(F144057P)
(F144057M)
Min.: 10
50
Max.: 100
± 0.35
± 0.40
± 0.80
≤ 0.10
≤ 0.12
≤ 0.15
± 0.8
± 0.8
± 0.8
≤ 0.3
≤ 0.3
≤ 0.3
P200G
(F144058P)
(F144058M)
Min.: 20
100
Max.: 200
± 0.50
± 0.80
± 1.60
≤ 0.20
≤ 0.25
≤ 0.30
± 1.6
± 1.6
± 1.6
≤ 0.6
≤ 0.6
≤ 0.6
P1000G
(F144059P)
(F144059M)
Min.: 100
500
Max.: 1000
± 3.0
± 4.0
± 8.0
≤ 0.6
≤ 1.0
≤ 1.5
± 8.0
± 8.0
± 8.0
≤ 3.0
≤ 3.0
≤ 3.0
P5000G
(F144066)
Min.: 500
2500
Max.: 5000
± 12
± 15
± 30
≤ 3
≤ 5
≤ 8
± 40
± 40
± 40
≤ 15
≤ 15
≤ 15
P10mL G
(F144067)
Min.: 1000
5000
Max.: 10000
± 30
± 40
± 60
≤ 6
≤ 10
≤ 16
± 60
± 60
± 60
≤ 30
≤ 30
≤ 30
Gilson’s Complete Pipetting System
Your protocols and experiments require more than just generic pipette tips. Indeed, tips
are essential components of your pipetting system. According to ISO 8655-2*:
“The maximum permissible errors always apply to the total system of piston pipette
and tip. When using pipette tips not delivered by the pipette supplier, the supplier
declaration or certificate of conformity does not apply”.
*ISO 8655 is an international standard for “piston operated apparatus”. It defines, among other things, terms and
definitions regarding piston operated pipettes and their testing, testing procedure and maximum permissible errors.
Pipette model
Recommended PIPETMAN DIAMOND tips
Model (scale 1/2) Volume range
P2G
D10 0.1 – 10 µL
DL10 0.1 – 20 µL
DF10 0.1 – 10 µL
DFL10 0.1 – 10 µL
P10G
D10 0.1 – 10 µL
DL10 0.1 – 20 µL
DF10 0.1 – 10 µL
DFL10 0.1 – 10 µL
P20G D200 2 – 200 µL
DF30 2 – 30 µL
P100G D200 2 – 200 µL
DF100 20 – 100 µL
P200G
D200 2 – 200 µL
D300 20 – 300 µL
DF200 20 – 200 µL
DF300 20 – 300 µL
P1000G
D1000 100 – 1000 µL
D1200 100 – 1200 µL
DF1000 100 – 1000 µL
DF1200 100 – 1200 µL
P5000 G D5000 1 – 5 mL
P10mL G D10mL 1 – 10 mL
PIPETMAN G kits complement the product range and offer
cost-effective package to get you started or to renew your
old set of pipette.
Pipette Kits
All you need to get started, in a single box!
PIPETMAN G Ordering reference Pipette Models* PIPETMAN DIAMOND Tips
STARTER KIT F167900 P20G - P200G - P1000G D200 - D1000
MICRO-VOLUME KIT F167800 P2G - P10G - P100G DFL10ST - DF100ST
*With both ejectors: stainless steel and plastic ejectors.
POS. 1 066803
POS. 2 066805
POS. 3 066807
POS. 4 066811
POS. 5 066813

Premium pipetting comfort!
EXTREMELY LOW PIPETTING FORCES!
EXTREMELY EASY TIP EJECTION!
LEGENDARY PIPETMAN ROBUSTNESS!
PIPETMAN G is a new mechanical, air-displacement pipette from Gilson.
PIPETMAN G oers you the lowest pipetting force on the market thanks to a piston
assembly system that gives you ideal pipetting and purge forces sensations.
Enjoy a new addition to PIPETMAN line with its legendary accuracy, precision, and
robustness… with the highest comfort!
TAKE COMFORT TO THE NEXT LEVEL
KEEP YOUR TECHNIQUE CONSISTENT
Use PIPETMAN G like the PIPETMAN Classic!
You can trust in your results and benefit from a great pipetting
experience. Don’t change your habits, even the ratio between
pipetting and purge forces is maintained!
Complete your equipment with:
Good Laboratory Practice (GLP) features ensure the high quality and reliability of your work.
CARROUSELTM pipette holder
(Ref. F161401)
SINGLETM pipette holder
(Ref. F161406)
TRACKMANTM
(Ref. F70301)
Model Ordering reference Volume Range Color code
Plastic ejector Stainless steel ejector
P2G F144054P F144054M 0.2 µL to 2 µL Orange
P10G F144055P F144055M 0.5 µL to 10 µL Red
P20G F144056P F144056M 2 µL to 20 µL Pale Yellow
P100G F144057P F144057M 10 µL to 100 µL Salmon
P200G F144058P F144058M 20 µL to 200 µL Yellow
P1000G F144059P F144059M 100 µL to 1000 µL Blue
Model Ordering reference Volume Range Color code
P5000G* F144066 500 µL to 5000 µL Purple
P10mL G* F144067 1 mL to 10 mL Light Blue
Vial of lubricant** 5440011070 For all models of PIPETMAN G
PIPETMAN G provides the legendary robustness and trustable accuracy in results of the PIPETMAN Classic.
It perfectly fulfils your expectations in terms of pipetting comfort, even for the most intensive pipetting
routine.
Thanks to the redesigned piston assembly (new seal, lighter springs) and the addition of high quality pis-
ton grease, you can enjoy one of the lowest* pipetting force available on the market!
GET A COMPLETE VOLUME RANGE
Get yourself a complete range of PIPETMAN G pipettes and benefit from the same
comfort, robustness and confidence in result from 2µL to 10mL!
CHOOSE YOUR LOOK:
CLASSIC OR MODERN DESIGN
Stainless steel or plastic ejectors are available to
you. They both have the legendary PIPETMAN
robustness and are equipped with a clip
ejector for a convenient access to the
serviceable parts.
When working with corrosive liquid we
recommend using the plastic ejector.
TRIOTM pipette holder
(Ref. F161405
EASY IDENTIFICATION
To easily distinguish your new PIPETMAN G from your PIPETMAN
Neo or Classic pipette, a blue ring has been placed under the
thumbwheel.
Blue ring
=
PIPETMAN G
Stainless steel ejector
Plastic ejector
C
l
i
p
e
j
e
c
t
o
r
*P5000G and P10mL G models do not require tip ejectors.
**PIPETMAN G can only be lubricated with a high quality lubricant selected and approved by Gilson. The use of other types of lubricants
will void the warranty for your pipette.
0,0
Competitor A
Volume Setting
Force (N.cm)
Newton
PIPETMAN NeoCompetitor B
Model (200 or 300 µL)
Competitor C PIPETMAN M
1,0
2,0
3,0
4,0
5,0
0
PIPETMAN G
Competitor A - Pipette 1
Competitor B - Pipette 1
Competitor A - Pipette 2
Competitor C - Pipette 1
Competitor A - Pipette 4
Competitor D - Pipette 1
Competitor A - Pipette 3
1
3
4
5
6
2
7
8
9
10
11
Competitor E - Pipette 1
PIPETMAN Neo
Competitor B - Pipette 2
Competitor E - Pipette 2
Competitor F
Competitor E - Pipette 3
Competitor B - Pipette 3
Competitor C - Pipette 2
Competitor A - Pipette 5
Competitor D - Pipette 2
Competitor C - Pipette 3
* Tested on Gilson and other brands of 200 µL pipette or equivalent.
POS. 1 066803
POS. 2 066805
POS. 3 066807
POS. 5 066813
POS. 4 066811

Caratteristiche tecniche pipette Gilson Pipetman
®
G
Pipette manuali a spostamento d’aria, a volume variabile.
•Gamma di volumi da 0.2 µl a 10 ml
•Forze di pipettaggio molto fluide grazie al nuovo pistone assemblato con molle e
guarnizioni più morbide.
•ndicatore di volume analogico a tre cifre.
•Regolazione in continuo del volume.
•L’espulsore può essere in plastica o in acciaio inox. L’espulsore in plastica è consigliato
quando si usano liquidi corrosivi e molto aggressivi.
•Numero di serie impresso a caldo sul corpo pipetta, dal quale si può risalire al mese e
all’anno di fabbricazione.
•Progettate, prodotte, calibrate e validate in conformità con le specifiche e i metodi
dello standard SO8655.
•Corredate di certificato di conformità originale Gilson.
•Conformi alla Normativa VD (98/79/EC) per gli strumenti n – Vitro Diagnostici.
•Garanzia 12 mesi.
Codici e specifiche tecniche
Modello
Codice
Volume
(µL)
Errori massimi tollerabili (µL)
Gilson ISO 8655
Errore Errore Errore Errore
Sistematico Casuale Sistematico Casuale
P2G
F144054P
F144054M
Min. 0.2
0.5
Max. 2
± 0.024 0.012
± 0.025 0.012
± 0.030 0.014
± 0.08 0.04
± 0.08 0.04
± 0.08 0.04
P10G
F144055P
F144055M
Min. 1
5
Max. 10
± 0.025 0.012
± 0.075 0.030
± 0.100 0.040
± 0.12 0.08
± 0.12 0.08
± 0.12 0.08
P20G
F144056P
F144056M
Min. 2
10
Max. 20
± 0.10 0.03
± 0.10 0.05
± 0.20 0.06
± 0.20 0.10
± 0.20 0.10
± 0.20 0.10
P100G
F144057P
F144057M
Min. 10
50
Max. 100
± 0.35 0.10
± 0.40 0.12
± 0.80 0.15
± 0.80 0.30
± 0.80 0.30
± 0.80 0.30
P200G
F144058P
F144058M
Min. 20
100
Max. 200
± 0.50 0.20
± 0.80 0.25
± 1.60 0.30
± 1.60 0.60
± 1.60 0.60
± 1.60 0.60
P1000G
F144059P
F144059M
Min. 100
500
Max. 1000
± 3 0.6
± 4 1.0
± 8 1.5
± 8 3.0
± 8 3.0
± 8 3.0
P5000G
F144066
Min. 500
2500
Max. 5000
± 12 3
± 15 5
± 30 8
± 40 15
± 40 15
± 40 15
P10mlG
F144067
Min. 1000
5000
Max. 10000
± 30 6
± 40 10
± 60 16
± 60 30
± 60 30
± 60 30
-Con una buona tecnica di pipettaggio la P2G può essere usata a 0.1 µL e la P10G a 0.5 µL.
-Nel codice la lettera P indica l’espulsore in plastica, mentre la lettera l’espuslore in metallo.
-La P5000G e la P10ml non hanno l’espulsore.
POS. 1 066803
POS. 2 066805
POS. 3 066807
POS. 5 066813
POS. 4 066811

Premium Pipetting Comfort!
IMPORTANT NOTICE!
This pipette can be only lubricated
with a high quality lubricant selected
and approved by Gilson. This lubricant is
availableunderthereference:5440011070.
The use of other type of lubricant cancels the
warranty of this pipette.

2
1  INTRODUCTION
PIPETMANGis afullyadjustableair displacementpipette
and is used with disposable tips. To answer the current
needs of our loyal users of ours pipettes, and still being
conform to Gilson brand qualities, three improvements
have been added to our mechanical series:
pipetting and purge forces dramatically decreased*,
large volumes, 5 mL and 10 mL very comfortable,
you have the choice for an ejector plastic or a stainless
steel ejector.
* Implementation of new features to improve dramatically
pipetting comfort has no impact on the legendary robustness,
accuracy and precision of PIPETMAN.
Eight single channel models cover a volume range from
0.2 µL to 10 mL.
Contents Page
1 - INTRODUCTION 2
2 - PaRTs CheCk LIsT 3
3 - DesCRIPTION 3
4 - seTTING The VOLUMe 4
5 - PIPeTTING 4
6 - GeNeRaL GUIDeLINes f OR GOOD PIPeTTING 7
7 - aCCess ORIes 8
8 - GLP fea TURes 9
9 - TROUbLesh OOTING 9
10 - Leak TesT 11
11 - MaINTeNaNCe 12
12 - CLeaNING aND DeCONTaMINaTION 15
13 - sPeCIf ICaTIONs 18
14 - sPaRe Pa RTs 20
eC DeCLaRaTION Of CONf ORMITY 22
WaRRaNTY 23

3
Decreased pipetting forces are due to the new de-
signedpistonassemblyincludingtheuseofavery
highqualityoflubricant.Theuseofotherlubricant
cancelsthewarrantyof thispipette.Thislubricant
is available under the reference: 5440011070.
Please contact your Gilson distributor.
2  PARTS CHECK LIST
Just take a moment to verify that the following items
are present:
PIPETMAN G, User's Guide,  Safety bag,
 Certificate of conformity (including bar-code sticker).
3  DESCRIPTION
Please refer to the following chapters for a full description of the different parts and functions of the pipette.
PIPeTMaN DIaMOND® tip
Tip-ejector
Tip-holder
handle
Tip-ejector button
Color-coded push-button
Thumbwheel
Connecting nut
blue ring

4
4  SETTING THE VOLUME
Thevolume ofliquidto beaspi-
ratedissetusingthevolumeter.
The dials are colored either
black or red to indicate the
position of the decimal point,
depending on the model (see
examples).
The volume is set by turn-
ing the thumbwheel or the
push-button.The push-button
makes it easier and quicker to
set volumes, especially when
wearing gloves. The thumb-
wheel may be turned using
only one hand to slowly reach
the required setting.
To obtain maximum accuracy when setting the volume,
proceed as follows:
whendecreasingthe volumesetting,slowlyreach the
requiredsetting,makingsurenottoovershootthemark.
whenincreasingthevolumesetting,pass therequired
valueby 1/3ofaturnandthenslowlydecreasetoreach
the volume, making sure not to overshoot the mark.
5  PIPETTING
For optimum performance, use of PIPETMAN DIAMOND
Tips with your PIPETMAN G is strongly recommended.
These tips, made from pure polypropylene have the
Model Color of volumeter numbers
Black Red Increment
P2G µL 0.01 µL 0.002 µL
P10G to P20G µL 0.1 µL 0.02 µL
P100G-P200G µL - 0.2 µL
P1000G 0.01 mL mL 0.002 mL
P5000G 0.01 mL mL 0.002 mL
P10mlG mL 0.01 mL 0.02 mL
Push-button Thumbwheeel
1
5
P2G
1.25 µL
2
0
7
5
P10G
7.5 µL
1
2
5
P20G
12.5 µL
P100G
0
7
5
75 µL
P200G
1
2
5
125 µL
P1000G
0
7
5
0.75 mL
P5000G
1
2
5
1.25 mL
P10mlG
0
7
5
7.5 mL

5
Gilson logo engraved on their collar, ensuring that you
have a genuine Gilson product. Plastic tips are for a
single application – they must not be cleaned for reuse.
PIPETMAN G can also be used with the main tip brands.
Fitting the tips
Tot anewPIPETMANDIAMONDTip,pushthe tip-holder
into the tip using a slight twisting motion to ensure a
firm, airtight seal.
For the P2G and P10G models equipped with stainless
steel tip-ejector,
A dual-position adapter (plastic) is
required to t DL10 tips (long tips) or
D10 tips (short tips).
P2G and P10G models are delivered
with the adapter in place, ready to
use DL10 tips. If D10 tips are used, the
adapter must be repositioned in the
shorter slot as follows:
Pull the adapter down from the
metallic rod.
Turn the adapter through 180°C.
Refit the adapter so that the end
of the metallic rod engages the
shorter slot of the adapter.
For the P2G and P10G models equipped with plastic
tip-ejector,
Atip-ejectorextensionissuppliedtot with
D10 tips (short tips).
To t a tip-ejector extension:
Slide the extension over the tip-holder.
Push the extension rmly onto the end
ofthe tip-ejectoruntilitclicksintoplace.
To remove a tip-ejector extension:
Gently twist the extension.
Pull it away from the pipette.
Both dual-position adapter and tip-ejector
extension are autoclavable .
Short tips Long tips

6
Pre-rinse the tips
Some liquids (e.g. protein-containing solutions and
organic solvents) can leave a lm of liquid on the inside
wall of the tip ; pre-rinse the tip to minimize any errors
that may be related to this phenomenon.
Pre-rinsingconsistsofaspiratingtherstvolumeofliquid
and then dispensing it back into the same vessel (or to
waste). Subsequent volumes that you pipette will have
levels of accuracy and precision within specications.
Aspirate
Press the push-button to the first stop (this
corresponds to the set volume of liquid).
Holdthepipetteverticallyandimmersethe tip in the
liquid (see immersion depth table, page 10). Release
the push-button slowly and smoothly (to top posi-
tion) to aspirate the set volume of liquid. Wait one
second (time depends on model, see table) ; then
withdraw the pipette-tip from the liquid. You may
wipe any droplets away from the outside of the tip
using a medical wipe, however if you do so take care
to avoid touching the tip’s orifice.
First stop
Second stop

7
Dispense
Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the
rst stop.
Waitforatleasta second,thenpressthepush-button
to the second stop to expel any residual liquid from
thetip.Keep thepush-buttonpressedfullydownand
(while removing the pipette) draw the tip along the
inside surface of the vessel.
Release the push-button, smoothly. Eject the tip by
pressing rmly on the tip-ejector button.
6 GENERAL GUIDELINES FORGOODPIPETTING
Makesurethatyouoperate
thepush-buttonslowlyand
smoothly.
When aspirating, keep the
tip at a constant depth
below the surface of the
liquid (refer to the table).
Change the tip before as-
pirating a dierent liquid,
sample, or reagent.
Change the tip if a droplet remains at the end of the
tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid
to be pipetted.
Liquid should never enter the tip-holder; to prevent
this:
- press and release the push-button slowly and
smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is liquid
in the tip.
If you use the same tip with a higher volume, pre-
rinse the tip.
Table - Immersion Depth
and Wait Time
Model Immersion Wait Time
Depth (mm) (seconds)
P2G 1 1
P10G 1 1
P20G 2-3 1
P100G 2-4 1
P200G 2-4 1
P1000G 2-4 2-3
P5000G 3-6 4-5
P10mlG 5-7 4-5

8
For volatile solvents you should saturate the air-
cushionofyourpipetteby aspiratinganddispensing
the solvent repeatedly before aspirating the sample.
When pipetting liquids with temperatures dierent
tothe ambient temperature,pre-rinse thetipseveral
times before use.
You may remove the tip-ejector (see Chapter 11 -
Maintenance) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids that
emit vapors, remove the tip-ejector, the tip-holder,
rinse, dry and lubricate the piston ( see Chapter 11
- Maintenance ). For the model P1000G, by using a
specic tip holder equipped with a lter, you can
increase the lifetime of the piston (see Chapter 7 -
Accessories).
Do not pipette liquids having temperatures above
70°Corbelow 4 °C.Thepipette can beusedbetween
+ 4 °C and + 40 °C but the specifications may vary
according to the temperature (refer to the ISO 8655-2
standard for conditions of use).
7  ACCESSORIES
To make pipetting more comfortable and more secure,
Gilson has developed several accessories:
To avoid the possibility of liquid running back into
the pipette, store the pipette vertically.
To identify or personalize your pipette, COLORIS™
clips are available:
COLORIs™ clips (mixed colors set of 10) f 161301
COLORIs™ clips (red, set of 10) f 161302
COLORIs™ clips (yellow, set of 10) f 161303
COLORIs™ clips (green, set of 10) f 161304
COLORIs™ clips (blue, set of 10) f 161305
COLORIs™ clips (white, set of 10) f 161306
CaRROUseL™ Pipette stand (7 pipettes) f 161401
TRIO™ stand (3 pipettes) f 161405
sINGLe™ pipette holder f 161406

9
WithTHE JIMMY™,hands freemicrotubeopener, you
can open both snap-cap and screw-cap microtubes.
To protect the piston when pipetting corrosive
liquids, you can use a specic tip holder and lter
for the model P1000G:
8  GLP FEATURES
The Serial Number is en-
graved on the body of the
pipette. It provides unique
identication of your pipette
and the date of manufacture.
Ex : AA10369
The Bar Code on the box and
the certificate of conformity
provide traceability of your
pipette.
9  TROUBLESHOOTING
A quick inspection of the pipette may help you to detect
a problem.
You maydownloadfrom theGilsonwebsite(www.gilson.com)
the “2 minute inspection”, which shows how to perform a quick
diagnosis of your pipette.
The JIMMY™ (set of 3) f 144983
Corrosion protection kit (tip holder + a bag of 10 filters) f 144570
Before returning any pipette to your local Gilson
Service Center, ensure that it is completely
free of chemical, biological, or radioactive
contamination. Refer to Chapter 12 - Cleaning
and Decontamination. Please use the included
safetybagtoreturnthepipettetoyourlocalGilson
Service Center.

10
The following table may help you to identify and correct
the problem you might encounter.
Symptom Possible Cause Action
Pipette is Damaged tip-holder Replace the tip-holder
leaking sample Worn O-ring or seal Replace both parts and lubricate
Pipette won’t Worn O-ring Replace both parts and lubricate
aspirate Damaged tip-holder Replace the tip-holder
Connecting nut is loose Tighten connecting nut
Damaged or corroded piston Return pipette to supplier
Improper repair or assembly see Chapter 11 - Maintenance
Pipette is Improper repair or assembly see Chapter 11 - Maintenance
inaccurate Unscrewed tip-holder Tighten connecting nut
Connecting nut is loose Tighten connecting nut
Pipette is Tip-holder is loose Tighten connecting nut
not precise Connecting nut is loose Tighten connecting nut
Incorrect operator technique Operator training
Damaged or corroded piston(s) Return pipette to supplier
Damaged tip-holder(s) Replace the tip-holder
Worn O-ring or seal Replace both parts and lubricate
Tips fall off or Low quality tips Use PIPeTMaN DIaMOND tips
do not fit Dirty tip-holder Clean the tip-holder with alcohol
correctly Damaged tip-holder(s) Replace the tip-holder
Damaged tip-ejector Replace tip-ejector
ejector spacer is missing Mount the spacer on the tip- ejector
The ejector spacer is damaged Replace the ejector spacer
The tip-ejector is loose assemble the tip-ejector properly
The ejector lock is misaligned align the ejector lock
Pipetting seize up Piston need lubricant Lubricate piston assembly
However, if you can’t solve the problem, contact your
Gilson distributor.

11
10  LEAK TEST
This test may be performed at any time to check that the
pipettedoes notleak,especiallyafterperformingamain-
tenance or decontamination procedure. If a pipette fails
this test, replace the O-ring and seal. After making sure
thatthepipette is correctly reassembled,repeat thistest.
For the P2G to P200G models:
Fit a PIPETMAN DIAMOND Tip.
Set the pipette to the maximum volume given in the
specifications, and pre-rinse.
Aspirate the set volume from a beaker of distilled
water.
Maintain the pipette in the vertical position and wait
for 20 seconds.
If a water droplet appears at the end of the tip there
is a leak.
If you see no droplet, re-immerse the tip below the
surface of water.
Thewaterlevelinsidethetip shouldremainconstant;
if the level goes down there is a leak.
For the P1000G, P5000G and P10mlG models:
Fit a PIPETMAN DIAMOND tip.
Set the pipette to the maximum volume given in
the specifications.
Aspirate the set volume from a beaker of distilled
water.
Maintain the pipette in the vertical position and wait
for 20 seconds.
If a water droplet appears at the end of the tip, there
is a leak.

12
11  MAINTENANCE
Routine maintenance will help keep your pipette in
good condition, ensuring a continued high level of
performance.
Maintenance is limited to:
Cleaningor decontamination(see Chapter12 -Cleaning
and Decontamination)
 Replacing spare parts
 Greasing the piston assembly.
PIPETMANP2G andP10Gshould notbedisassembled,
so you may only replace the push-button, tip-ejector,
dual position tip-ejector and its adapter. With these
pipettes if the tip-holder is damaged, the piston may
also be damaged.
After replacing any parts you should verify
the performance of your pipette following the
verification procedure available on the Gilson
website (www.gilson.com). If the pipette needs
to be readjusted, please contact your local Gilson
authorized Service Center.
Changing the Tip-ejector
To remove
Pushtheejection
button.
Pushlaterallythe
tip-ejector.
Slideand remove
the tip-ejector.
To ret
Pushtheejection
button.
Slide the tip-ejector along the tip-holder.
Clip the tip-ejector on the body of the pipette.

13
P10G, P20G
P100G, P200G
P5000G
P10mlG
P1000G
Tip-holder
Tip-ejector
stainless steel plastic
Connecting nut
Color coded push-button
Piston
assembly
Blue ring

14
Changing the Tip-holder – no tools required
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter-
clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly and the seals.
Clean, autoclave, or replace the tip-holder.
If necessary, lubricate lightly the piston and its seals
(see below).
Reassemble the pipette (refer to the figure, p 16).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
Servicing the Piston Assembly
You may remove the piston assembly for cleaning pur-
posesonly. If the pistonassemblyischanged, the pipette
must be adjusted and calibrated in a Gilson authorized
Service Center. As the models P2G and P10G contain
miniaturized parts, it is best not to disassemble these
pipettes yourself.
The piston assembly must not be autoclaved.
Remove the tip-ejector (see above).
Unscrew the connecting nut by
turning it counter- clockwise.
Carefully separate the lower and
upper parts.
Removethe pistonassemblyand
the seals.
Leave exposed the piston, clean
it with isopropanol or ethanol
and lubricate lightly. For P100G,
P200G and P1000G models, lu-
bricate only the useful part of the
piston and the seals. For P5000G
and P10mlG, lubricate the tip of
the piston, disassemble the seals
and lubricate their internal part.

15
The use of other lubricant cancels the warranty
of this pipette.
Reassemble the pipette (refer to the figure, page 13).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
Changing the seals
The O-ring and seal are on the piston; they must not be
autoclaved, if worn or damaged in any way (chemical
or mechanical), they must be replaced. As the models
P2G and P10G contain miniaturized parts, it is best not
to disassemble these pipettes yourself, please contact
your local Gilson authorized Service Center.
The dimensions of the O-ring vary depending on the
model of pipette.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter-
clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
If necessary clean the piston and replace the seal;
lubricate them lightly. Please place them in the
correct order.
Reassemble the pipette (refer to the figure, page 13).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
12  CLEANING AND DECONTAMINATION
PIPETMAN G is designed so that the parts normally in
contact with liquid contaminants, can easily be cleaned
and decontaminated. However, because the models
P2G and P10G contain miniaturized parts, it is best not
to disassemble these pipettes yourself; please contact
your local Gilson authorized Service Center.

16
You may refer to the decontamination procedure
availableontheGilsonwebsite(www.gilson.com).
Liquidmustnever entertheupperpart (handle)
of any pipette.
Cleaning
The pipette must be cleaned, as described below, before
it is decontaminated. Soap solution is recommended for
cleaning PIPETMAN G.
External
Remove the tip-ejector.
Wipe the tip-ejector with a soft-cloth or lint-free
tissue impregnated with soap solution.
Wipe the entire pipette with a soft-cloth or lint- free
tissueimpregnatedwith soap solution, to removeall
dirty marks. If the pipette is very dirty, a brush with
soft plastic bristles may be used.
Wipethe entirepipetteandthetip-ejector withasoft
cloth or lint-free tissue soaked with distilled water.
Refit the tip-ejector and allow the pipette to dry.
Internal
The following components only can be immersed in a
cleaning solution: connecting nut, tip-ejector, tip-holder,
piston assembly, seal and O-ring.
Disassemble the pipette as described in the Chapter
11 - Maintenance.
Set aside the upper part in a clean, dry place.
Clean the individual components of the lower part
of the pipette using an ultrasonic bath (20 minutes
at 50°C) or with a soft-cloth and brushes. Note that
the piston assembly and seals must be degreased
with isopropanol or ethanol before being immersed
in the ultra sonic bath. Small round brushes with soft
plastic bristles may be used to clean the interior of
the tip-holder.
Rinsetheindividualcomponentswithdistilledwater.
Leave the parts to dry by evaporation or wipe them
with a clean soft-cloth or lint-free tissue.

17
Reassemble the pipette as described in the Chapter
11 - Maintenance.
Autoclaving
The upper part (body) and the piston assembly of the
pipette are not autoclavable. Only the following parts
maybeautoclaved:tip-ejector,tip-holderandconnecting
nut. The O-ring and seal are not autoclavable; they may
be cleaned or replaced with the one specified in Chapter
14 - Spare Parts.
Clean the parts to be autoclaved, especially the
tip-holder.
Put the parts in an autoclaving sack.
Autoclave for 20 minutes at 121°C, 0.1 MPa.
Check that the parts are dry before re-assembling
the pipette.
Setthepipetteasidetostabilizeatroomtemperature.
Chemical Decontamination
You may choose to decontaminate your pipette chemi-
cally, in accordancewithyourownprocedures.Whatever
decontaminant you use, check with the
supplier of the
decontaminant that it is compatible with stainless steel
and the plastics used in the construction of the pipette:
PA (Polyamide), PBT (Polybutylene Terephtalate), PC
(Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene
Terephtalate), POM (Polyoxymethylene), or PVDF (Poly-
vinylidene Fluoride).
Upper Part (handle)
Wipe the upper part (handle) of the pipette with a
soft-cloth or lint-free tissue impregnated with the
chosen decontaminant.
Wipe the upper part of the pipette with a soft- cloth
or lint-free tissue soaked with distilled water or
sterile water.
Lower Part (Volumetric module)
The following components only can be immersed in a
decontaminant solution: connecting nut, tip-ejector,
tip-holder.