Gilson MICROMAN E M25E User manual

User’s Guide
EN
E

TABLE OF CONTENTS
1TABLE OF CONTENTS |MICROMAN E®
TABLE OF CONTENTS
INTRODUCTION | 2
Parts Checklist | 3
Good Laboratory Practice (GLP) Features | 3
Description | 4
Operating Ranges and Material | 5
Specifications | 6
FITTING THE CAPILLARY PISTON | 8
PIPETTING | 10
Setting the Volume | 10
Aspirating | 11
Dispensing | 11
Ejecting the Capillary Piston | 12
TROUBLESHOOTING | 13
CLEANING AND DECONTAMINATION | 14
WARRANTY | 17
Before use, please carefully read
WARRANTY on page 17 of this
User’s Guide!

INTRODUCTION
2
MICROMAN E® |INTRODUCTION
TABLE OF CONTENTS |MICROMAN E®
Chapter 1
INTRODUCTION
Congratulations on purchasing your new
MICROMAN E®pipette. MICROMAN E is suitable
for many different applications, including
pipetting volatile, viscous, dense, or high surface
tension liquids. It is a quality product from Gilson,
which is fully compliant with ISO 8655 standards.
The six models available allow for precise and
accurate pipetting of viscous, dense, and volatile
liquids. They also enable contamination-free
pipetting, preventing vapor contamination and
cross-contamination. M25E, M50E, and M250E,
with thin and tall capillary pistons (CPs) are
especially suited for use with tall or narrow
vessels.
Enjoy the following features:
●MICROMAN E pipettes are equipped with a
positive displacement mechanism isolating
the aspirated liquid from the pipette body.
The positive displacement mechanism
prevents the sample-to-sample contamination
that can result from the aerosol effect.
●MICROMAN E uses disposable capillary
pistons. These are the only parts that contact
with the aspirated liquids, and replacement
thereof will provide absolute protection
against contamination caused by carryover
between samples or reagents. Simply replace
the capillary piston between assays; they are
ejected automatically and simultaneously,
thus avoiding any risk for the operator.
●MICROMAN E is equipped with a direct
reading volumeter that allows precise
adjustment of the required volume. At the
manufacturing stage, each MICROMAN E is
permanently adjusted to the corresponding
capillary piston. The capillary piston is
positioned automatically, and therefore
no further adjustment is requested when
replacing it.
●MICROMAN E requires no lubrication, due to
the nature and quality of the materials used in
its component parts.

INTRODUCTION
3INTRODUCTION | MICROMAN E®
Parts Checklist
Verify that the following items are present:
●MICROMAN E,
●User’s Guide,
●Safety bag,
●Adhesive ID-tags (strip of 6),
●Capillary pistons (10),
●Certificate of conformity
(including barcode sticker).
Good Laboratory Practice (GLP)
Features
●No need to touch disposables
(capillary piston).
●Serial Number: engraved on
pipette body.
●Barcode: on the box and
with the certificate (can be
transferred).
●ID Tag (Application or User).
●Color-coded push button
indicating the volume range.
●Data matrix engraved on
pipette body.
●Gilson certificate of
conformity according
to ISO 8655.
Figure 1
Data Matrix and
Serial Number
Location

INTRODUCTION
4
MICROMAN E® |USER’S GUIDE
INTRODUCTION | MICROMAN E®
Description
Color-coded push button
indicating the volume
range for aspirating and
dispensing.
Body or handle
Capillary
Piston
Shaft
Thumbwheel for
setting the volume
Volumeter
Volume unlock button
Data matrix code
1
2
3
4
5
6
7
8
9
1
2
3
4
5
6
7
8
9
Figure 2
MICROMAN® E

Operating Ranges and Material
Available Models
Materials
INTRODUCTION
5INTRODUCTION | MICROMAN E®
MODEL VOLUME RANGE
M10E 1 µL to 10 µL
M25E 3 µL to 25 µL
M50E 20 µL to 50 µL
M100E 10 µL to 100 µL
M250E 50 µL to 250 µL
M1000E 100 µL to 1000 µL
MODEL SPRING CLAMP SHAFT BODY (B)
M10E SS Be Alloy PBT PVDF/PP
M25E SS Be Alloy PVDF PVDF/PP
M50E SS Be Alloy PVDF PVDF/PP
M100E SS Be Alloy PBT PVDF/PP
M250E SS Be Alloy PVDF PVDF/PP
M1000E SS Be Alloy PVDF PVDF/PP
CP CAPILLARY (C) PISTON (P)
CP10 Polypropylene Polyester
CP25 Polypropylene Polyester
CP50 Polypropylene Polyester
CP100 Polypropylene Polyethylene
CP250 Polypropylene Polyethylene
CP1000 Polypropylene Polyacetal
Abbreviations
SS = Stainless Steel
Be = Beryllium
PBT = Polybutylene Terephthalate
PP = Polypropylene
PVDF = Polyvinylidene Fluoride

INTRODUCTION
6
MICROMAN E® |USER’S GUIDE
INTRODUCTION | MICROMAN E®
Specifications
MICROMAN E is a high quality pipette that offers
excellent accuracy and precision. The figures
provided in the “ Gilson Maximum Permissible
Errors ” table were obtained using Gilson CPs.
These figures are only guaranteed when using
genuine Gilson CPs.
Each pipette is inspected and validated by qualified
technicians according to the Gilson Quality System.
Gilson declares that its manufactured pipettes
comply with the requirements of the ISO 8655
Standard, by type testing. The adjustment is carried
out under strictly defined and monitored conditions
(ISO 8655-6):
●Basis of adjustment, Ex.
●Reference temperature, 20°C
●Relative humidity, 50%
●Barometric pressure, 101 kPa
●Use of distilled water grade 3 (ISO 3696)
●Ten measurements for each test volume,
which are nominal volume, 50% of nominal
volume, and the minimum or 10% of nominal
volume.
Performance Tests
Each pipette is inspected and validated according
to the Gilson Quality Assurance System. Based
on extensive historical data, manufacturing
conditions, and expertise, and in compliance with
ISO standards relative to statistical process control,
the assurance level of this instrument performing
to specifications is 99.8 %. Specifications rely on
quality and consistency for the whole pipetting
system and are guaranteed only when the pipette is
used with Gilson capillary pistons.

MAXIMUM PERMISSIBLE ERRORS
MODEL
(PART
NUMBER)
VOLUME
( µ L)
GILSON ISO 8655
SYSTEMATIC
ERROR
(µL)
RANDOM
ERROR
(µL)
SYSTEMATIC
ERROR
(µL)
RANDOM
ERROR
(µL)
M10E
(FD10001)
Min. 1 ±0.09 ≤0.03 ±0.2 ≤0.1
5 ±0.10 ≤0.03 ±0.2 ≤0.1
Max. 10 ±0.15 ≤0.06 ±0.2 ≤0.1
M25E
(FD10002)
Min. 3 ±0.25 ≤0.08 ±0.7 ≤0.3
10 ±0.27 ≤0.08 ±0.7 ≤0.3
Max. 25 ±0.30 ≤0.10 ±0.7 ≤0.3
M50E
(FD10003)
Min. 20 ±0.34 ≤0.20 ±0.7 ≤0.3
Max. 50 ±0.70 ≤0.30 ±0.7 ≤0.3
M100E
(FD10004)
Min. 10 ±0.50 ≤0.20 ±1.5 ≤0.6
50 ±0.75 ≤0.30 ±1.5 ≤0.6
Max. 100 ±1.00 ≤0.40 ±1.5 ≤0.6
M250E
(FD10005)
Min. 50 ±1.50 ≤0.30 ±6 ≤2.0
100 ±1.70 ≤0.30 ±6 ≤2.0
Max. 250 ±2.50 ≤0.50 ±6 ≤2.0
M1000E
(FD10006)
Min. 100 ±3.0 ≤1.6 ±12 ≤4.0
500 ±5.0 ≤2.5 ±12 ≤4.0
Max. 1000 ±8.0 ≤4.0 ±12 ≤4.0
INTRODUCTION
7INTRODUCTION | MICROMAN E®
Gilson Maximum Permissible Errors
The data given in the table conform to the
ISO 8655-2 Standard.

MAXIMUM PERMISSIBLE ERRORS
MODEL
(PART
NUMBER)
VOLUME
( µ L)
GILSON ISO 8655
SYSTEMATIC
ERROR
(µL)
RANDOM
ERROR
(µL)
SYSTEMATIC
ERROR
(µL)
RANDOM
ERROR
(µL)
M10E
(FD10001)
Min. 1 ±0.09 ≤0.03 ±0.2 ≤0.1
5 ±0.10 ≤0.03 ±0.2 ≤0.1
Max. 10 ±0.15 ≤0.06 ±0.2 ≤0.1
M25E
(FD10002)
Min. 3 ±0.25 ≤0.08 ±0.7 ≤0.3
10 ±0.27 ≤0.08 ±0.7 ≤0.3
Max. 25 ±0.30 ≤0.10 ±0.7 ≤0.3
M50E
(FD10003)
Min. 20 ±0.34 ≤0.20 ±0.7 ≤0.3
Max. 50 ±0.70 ≤0.30 ±0.7 ≤0.3
M100E
(FD10004)
Min. 10 ±0.50 ≤0.20 ±1.5 ≤0.6
50 ±0.75 ≤0.30 ±1.5 ≤0.6
Max. 100 ±1.00 ≤0.40 ±1.5 ≤0.6
M250E
(FD10005)
Min. 50 ±1.50 ≤0.30 ±6 ≤2.0
100 ±1.70 ≤0.30 ±6 ≤2.0
Max. 250 ±2.50 ≤0.50 ±6 ≤2.0
M1000E
(FD10006)
Min. 100 ±3.0 ≤1.6 ±12 ≤4.0
500 ±5.0 ≤2.5 ±12 ≤4.0
Max. 1000 ±8.0 ≤4.0 ±12 ≤4.0
FITTING THE CAPILLARY PISTON
8
MICROMAN E® |USER’S GUIDE
INTRODUCTION | MICROMAN E®
Chapter 2
FITTING THE CAPILLARY
PISTON
Make sure the capillary piston
(CP) is compatible with the
MICROMAN E model that will
be used.
CP25, CP50, and CP250 Bulk
The capillaries and pistons
are delivered in two separate
boxes. To fit a capillary piston:
1. Take care not to
damage the sealing
tip when handling
the piston .
2. Select a piston
and slide it into the
capillary .
3. Gently push the
capillary until it snaps
onto the capillary
holder.
4. Attach the stem (
by slowly pressing
the push button until
you feel and hear a
slight click and then
continue to press to
the first stop. Finally,
pipette the liquid.
CP MODEL
CP10 M10E
CP25 M25E
CP50 M50E
CP100 M100E
CP250 M250E
CP1000 M1000E
1
3
3
4
2
1
4
3
2
Figure 3
MICROMAN
Capillary Piston
Never lubricate the capillary holder,
capillary, or any other part of the
pipette. If the capillary tends to slip
o the capillary holder, clean the
capillary holder with ethanol, using
a medical wipe or similar soft tissue.

FITTING THE CAPILLARY PISTON
9FITTING THE CAPILLARY PISTON | MICROMAN E®
CP10, CP25, CP50, CP100, CP250, and
CP1000 Tipack
Capillary pistons (CP) are delivered ready to use.
To fit a capillary piston:
1. Press the
MICROMAN E onto
the capillary piston
until it is firmly
seated .
2. Pick up the CP
from the rack .
3. Slowly press the
push button until
you feel and hear
a slight click and
continue to press
to the first stop .
Then pipette the
liquid.
4. Press the push button
on the second stop to
eject the CP.
1
1
2
2
3
3
Figure 4
CP fitting on MICROMAN®E