Grant-bio ES-20/80 User manual

Orbital shaker-incubator
ES-20/80
Operating Manual

Edition 1.04 –December of 2021 Orbital shaker-incubator ES-20/80
Page 2 Operating manual

Orbital shaker-incubator ES-20/80 Edition 1.04 –December of 2021
Operating manual Page 3
Contents
1. About this edition of user instructions .....................................................................................3
2. Safety precautions..................................................................................................................4
3. General information................................................................................................................5
4. Getting started........................................................................................................................6
5. Stacking.................................................................................................................................8
6. Operation.............................................................................................................................10
7. Specifications.......................................................................................................................12
8. Ordering information.............................................................................................................13
9. Guarantee and service.........................................................................................................14
10. Compliance..........................................................................................................................15
1. About this edition of user instructions
The current edition of the user instructions applies to the following models and versions of the
orbital shaker-incubator:
•ES-20/80....................................................................................................................... V.1GD

Edition 1.04 –December of 2021 Orbital shaker-incubator ES-20/80
Page 4 Operating manual
2. Safety precautions
Caution!
Make sure you have fully read and understood the present Manual before using
the equipment. Please pay special attention to sections marked by this symbol.
Caution!
Hot surface! Incubator surface and inner camera may become very hot during
use. Always use protective cotton gloves to install or remove samples when the
temperature is set higher than 60°C.
GENERAL SAFETY
•The protection provided can be ineffective if the operation of the appliance does notcomply with
the manufacturer's requirements.
•Save the unit from shocks and falling.
•After transportation, storage or in the case of condensation formation, and before connecting it
to the electric circuit, keep the unitunder room temperature for 2-3 hrs todry outthe unit. Without
drying out, the operation of the unit may not comply with all safety requirements.
•Store and transport the unit at ambient temperatures between -20°C and +60°C and maximum
relative humidity of 80%.
•Before using any cleaning ordecontaminationmethods except thoserecommended by the man-
ufacturer, check with the manufacturer that the proposed method will not damage the equip-
ment.
•Do not make modifications in design of the unit.
ELECTRICAL SAFETY
•Connect only to the mains with voltage corresponding to that on the serial number label.
•Do not plug the unit into an ungrounded power socket, and do not use an ungrounded extension
lead.
•Ensure that the power plug is easily accessible during use.
•Disconnect the unit from the mains before moving.
•If liquid penetrates into the unit, disconnect it from the mains and have it checked by a repair
and maintenance technician.
•Do not operate the unit in premises where condensation can form. Operating conditions of the
unit are defined in the Specifications section.
DURING OPERATION
•Make sure that all sample vessels are tightly sealed. Humidity caused by evaporation from un-
sealed vessels inside the incubator will damage the unit.
•Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please
contact manufacturer for possible operation of the unit in specific atmospheres.
•Do not incubate flammable, explosive or chemically active substances. If such substance is
spilled inside the incubator, it must be cleaned with a moist cloth and a mild soap solution.
•Do not operate the unit if it is faulty or has been installed incorrectly.
•Do not use outside laboratory rooms.
•Do not place a loadexceedingthe maximum loadvalue mentionedin the Specifications section
of this manual.
BIOLOGICAL SAFETY
•The user is responsible to carry out appropriate decontamination if hazardous material spills on
or penetrates into the equipment.

Orbital shaker-incubator ES-20/80 Edition 1.04 –December of 2021
Operating manual Page 5
3. General information
ES-20/80 shaker-incubator for biotechnological and pharmaceutical laboratories is a profes-
sional category equipment. The typical applications include - microbial and cell culture cultivation, pro-
tein expression, solubility studies, general mixing, as well as other various applications in the fields of
biology and chemistry. The unit is equipped with a newly developed triple eccentric mechanism for plat-
form motion that provides supreme balancing characteristics, superior reliability and quiet operation.
The achieved stability of the unit during vigorous mixing allows for stacking installation of up to 3 units
which enables to save space.
The new display and easy to use user interface provide a clear and intuitive control of parame-
ters and allow data logging, storage and display over time. Additionalfeatures like out of balance sensor
and automatic thermostat failure detection make this shaker-incubator an advanced and safe product.
Bluetooth connectivity to PC allows for data management, data logging, parameter control and profiling
in a dedicated software that can be requested separately.
A built-in heat-resistant brushless fan provides precise temperature distribution inside the cham-
ber (from 5°C above ambient up to +80°C). Additionally, excellent sample temperature uniformity of ±0.5
°C at 37 °C is achieved. The inner chamber is made of stainless steel. State-of-the-art motor, thermal
insulation materials and parameter PID-control decrease the energy consumption and make the shaker-
incubator highly energy efficient despite its relatively large size.

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Page 6 Operating manual
4. Getting started
4.1. Unpacking. Remove packing materials carefully and retain them for future shipment or storage of
the unit. Examine the unit carefully for any damage incurred during transit. The warranty does not
cover in-transit damage. Warranty covers only the units transported in the original package.
Caution!
Due to the high weight of the unit, its unpacking and installing must be carried out
by two persons.
Caution!
After transportation, storage or in the case of condensation formation, and before
connecting it to the electric circuit, keep the unit under room temperature for 2-3
hrs to dry out the unit. Without drying out, the operation of the unit may not com-
ply with all safety requirements.
4.2. Complete set. Package contents:
4.2.1. Standard set:
- ES-20/80, Orbital shaker-incubator.................................................................................1 pce.
- Power cable ..................................................................................................................1 pce.
- Spare fuse (inside fuse holder) ...................................................................................... 1 pce.
- Four screws and a hex driver ........................................................................................... 1 set
- USB drive with software and software manual................................................................1 pce.
- Operating instructions, declaration of conformity ...........................................................1 copy
4.2.2. Optional accessories:
- HSP-30/100 platform............................................................................................... on request
- HSP-16/250 platform............................................................................................... on request
- HSP-9/500 platform................................................................................................. on request
- HSP-6/1000 platform............................................................................................... on request
- PP-400 platform ...................................................................................................... on request
- UP-168 universal platform....................................................................................... on request
- HSC-50, HSC-100, HSC-250, HSC-500, HSC-1000 clamps for UP-168.................. on request
- TR-21/50 test tube rack for UP-168......................................................................... on request
- TR-44/15 test tube rack for UP-168......................................................................... on request
- USB Bluetooth adapter for PC................................................................................. on request
- Stacking kit for two units..........................................................................................on request
- Stacking kit for three units ....................................................................................... on request
HSP-30/100 HSP-16/250 HSP-9/500

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Operating manual Page 7
HSP-6/1000 PP-400 UP-168
TR-21/50 TR-44/15
4.3. Setup.
Note.
Ensure that the unit is placed on level horizontal surface, which is able to support
its weight.
- Place the unit on an even horizontal stable non-flammable surface 30 cm away from any flamma-
ble materials, clear 20 cm around the unit on all sides for ventilation.
- Level the unit on the surface by adjusting the threaded legs. Rotate the legs until the unit is
stable, then secure the thread with a jam nut. The unit should stand on all 4 legs, vibrations
should be absent.
- Remove the protective film from the display.
- Connect the power cable to the socket on the rear side of the unit and position it with easy ac-
cess to the power switch and plug.
4.4. Platform installation.
4.4.1. Remove the silicon mat and unscrew four screws from the previous platform using a hex driver, if a
platform was present. Install and secure the new platform to the moving part of the base with four
screws and replace the silicone mat.
4.4.2. UP-168 platform. The platform can be outfitted with flask clamps or a test tube rack. Arrange the
components symmetrically in relation to the center of the platform. Fix them in place with screws
included with components. Maximum number of clamps or racks can be found in the table below.
FC-50
36
FC-100, FC-250
16
FC-500
8
FC-1000
4
TR-21/50, TR-44/15
2

Edition 1.04 –December of 2021 Orbital shaker-incubator ES-20/80
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5. Stacking
Note.
The following section applies to the optional stacking kit, to use with 2 or 3 shaker-
incubator units.
5.1. Stacking kit contains:
Component
Kit for 2 units
Kit for 3 units
Catalogue number
BS-0101670-OK
BS-0101670-PK
Side legs
–
2
Holder plate
4
8
Hex screw 6x16
16
40
Washer
16
40
Lock washer
16
40
Hex key
1
1
5.2. Aquiring a kit. To purchase the kit, contact Boeco or your local Boeco representative, with infor-
mation from the section Ordering information.
5.3. Getting started.
5.3.1. Unpack the kit. Unpower the units and disconnect the power cables.
5.3.2. Prepare the working space. Ensure that the floor and the desk supports at least 150 kgF/m2(for 2
units) or 200 kgF/m2(for 3 units). Laminate or rubber flooring may reduce overall stability and are
generally not recommended.
5.3.3. Position the units so that the access tothe mains plugs, the sockets andthe power switches is unob-
structed.
5.4. Stacking two units (figure 2).
5.4.1. Set one unit atop the other.
5.4.2. Connecttheunitswithfourplates.Aplaterequires fourscrews,eachwithawasherandalockwasher
on it (fig. 3). Fix the screws tightly.
5.5. Stacking three units.
5.5.1. Base unit (figure 4):
5.5.2. Attach the left and the right legs to the base unit. A leg requires four screws, each with a washer and
a lock washer on it (fig. 3). Fix the screws tightly.
5.5.3. Regulate the rubber parts of the legs so that the base unit is stable and level.
5.5.4. Middle unit (figure 5):
5.5.5. Set the middle unit atop of the base unit.
5.5.6. Connecttheunitswithfourplates.Aplaterequires fourscrews,eachwithawasherandalockwasher
on it (fig. 3). Fix the screws tightly.
5.5.7. Top unit (figure 6):
5.5.8. Set the top unit atop of the middle unit.
5.5.9. Connecttheunitswithfourplates.Aplaterequires fourscrews,eachwithawasherandalockwasher
on it (fig. 3). Fix the screws tightly.
5.6. Connect the power cables to the units and to grounded mains sockets.
5.7. Maintenance. Check the screw fixation and unit stack stability once a month.

Orbital shaker-incubator ES-20/80 Edition 1.04 –December of 2021
Operating manual Page 9
Figure 2. Stacking two units Figure 3. Washers on the screw
Figure 4. Stacking three
units, base level
Figure 5. Stacking three
units, middle level
Figure 6. Stacking three
units, top level

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6. Operation
6.1. Connecttheunittoagroundedpowersocket.SetthePowerswitch
on the rear panel to position I(on).
6.2. The display turns on. From top to bottom, display shows:
- Current date and time;
- Actual and set temperatures;
- Actual RPM, set RPM and timer;
- Status bar;
- Context buttons, corresponding to unmarked keys below the
display.
6.2.1. Below the display:
- Four unmarked keys, corresponding to context buttons on the
display;
- The Select, ▲, ▼ and Run Stop keys.
6.3. Settingtheparameters.Pressthe Select keytoselecttheparam-
eter to change. Use the ▲ and ▼ keys to adjust the parameter.
Pressing the key for longer than 2 s increases changing speed.
Press the Select key againto select the next parameter. Changes
are saved automatically.
6.3.1. Setting temperature. Temperature starts changing according to
set value. Increment is 0.1ºC. Values can be changed anytime.
6.3.2. Setting speed (RPM). Increment is 10 RPM. Speed can be ad-
justed anytime.
6.3.3. Setting time. Time is set separately for hours and minutes, with
increment of 1 minute.
6.4. Program execution.
6.4.1. When the actual temperature in the incubator reaches set values, open the door and place samples
on the platform.
Caution!
Place the samples symmetrically in relation to the center of the platform to avoid
imbalance errors.
Caution!
Do not fill the vessels inside the incubator. Please make sure that all vessels are
tightly sealed. Humidity caused by evaporation from unsealed vessels inside the
incubator will damage the unit!
6.4.2. Press the Run Stop key. The platform will start rotating and the timer indicator will start counting the
time interval.
Caution!
If the speed is set to zero, pressing the Run Stop key will start the timer, but the
platform will not move.

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6.5. After finishing the program (after the set time elapses), the platform motion stops and the status bar
shows STOP accompanied by the repetitive sound signal. Press any key to stop the signal.
Caution!
The chamber heating can only be stopped manually by reducing the temperature.
6.6. If the time is not set (set time is 00:00), the Run Stop key will start continuous operation of the unit
until the Run Stop key is pressed again.
6.7. The platform motion can be stopped at any time by pressing the Run Stop key. In this case, the
program realisation and the platform motion will stop and the timer will switch into the STOP mode
saving previously set time. Press the Run Stop key to restart the operation with the same time and
speed. Additionally, if the door is opened the platform will stop; after closing the door, platform will
reinitiate motion automatically.
6.8. Incubator menu. The Menu button opens incubator’s menu. Use ▲ and ▼ buttons to navigate and
Enter button to select. While browsing in the menu, select the topmost Quit option in order to return
from sub-menu to menu.
6.8.1. Incubator ID. Option GetID shows the serial number and name of the incubator and its Bluetooth
module MAC address, as well as remote PC Bluetooth module MAC address.
6.8.2. Date & Time. To set dateand timein this submenu, press Enter button to select the parameter, then
▲ and ▼ buttons to change the value and Enter button to confirm changes.
6.8.3. Service. This submenu has following options: Current Errors and Settings.
6.8.3.1 Current Errors. This option allows user to view and reset the list of active errors.
6.8.3.2 Settings. This option has two suboptions available –Alarm Setting and Start mode.
•Alarm Setting. User can set the alarm settings based on values of temperature and RPM. All
alarms are working on the following principle, temperature taken as an example:
If Tact < Tthr.low or Tact > Tthr.high for tdelay minutes, then an alarm will be triggered.
Where Tact is the actual temperature inside the incubator, Tthr.low is the lower threshold limit value,
Tthr.high is the higher threshold limit value and tdelay is the time delay in minutes. Time delay is
used in order to avoid false triggering of the alarms.
RPM alarm is operated using the same principle.
•Start Mode. User can change the acceleration mode of the unit. In Start Mode Slow the unit
picks up speed gently to reduce the possibility of spilling. In Start Mode Fast the unit rapidly
gains speed and set RPM rate.
6.8.4. Active Alarms. This option allows user to view and reset the list of active alarms.
6.8.5. Data Logger. Data logger features following options: View Plot T(t), View Plot RPM(t), View Plot
T(t)+RPM(t), Clear log, Logger on/off.
6.8.5.1 All View Plot options use the same interface. Use Left and Right buttons to move the plot along the
x axis. Use Up andDown buttons to movetheplot along the y axis. Use <-xx-> button tochoosethe
x axis navigational scale. Available scales are 15 minutes, 1 hour, 1 day, 1 week and 1 month.
6.8.5.2 Clear Log button clears the logger’s data.
6.8.5.3 Logger On/Off button toggles the automatic logging of the data.
6.8.5.4 Availablememory indicator.Whenthedataloggeruses upallavailable memory,itbegins overwrit-
ing data from beginning. 100% of memory is used up in approximately 1 month.
6.9. At the end of operation, set the Power switch in position O (off). Disconnect the power cable from
electric circuit.

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7. Specifications
The unit is designed for operation in cold rooms and closed laboratory rooms at ambient tem-
perature from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for
temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.
Grant is committed to a continuous programme of improvement and reserves the right to alter
design and specifications of the equipment without additional notice.
7.1. Temperature specification
Setting range ..................................................................................+25°C ... +80°C (increment 0.1°C)
Control range .......................................................................................... 5°C above ambient ... +80°C
Uniformity
1
.................................................................................................................................±0.3°C
Accuracy1..................................................................................................................................±0.1°C
Stability1.....................................................................................................................................±0.1°C
Heat up time to +80°C inside the chamber ................................................................................ 75 min
7.2. General specification
Speed control range........................................................................50 - 400 RPM (increment 10 RPM)
Digital time setting ..............................................................1 min - 96 hrs (increment 1 min) / non-stop
Maximum continuous operation time
2
........................................................................................30 days
Maximum load.......................................................................................................................... 10.6 kg
Orbit 20 mm
Display .................................................................................................................................. TFT LCD
PC software......................................................................................................................... on request
Data transfer..........................................................................................................................Bluetooth
Dimensions ..............................................................................................................620x620x510 mm
Dimensions of the inner chamber..............................................................................460x350x400 mm
Stacking............................................................................................................................up to 3 units
3
Operating voltage/ power consumption...............................................230 V, 50/60 Hz / 500 W (2.2 A)
Weight
4
48 kg
Table 1. Recommended maximum speed for UP-168 platform
with fully loaded TR-21/50 or TR-44/15, depending on the set angle.
Set angle
Speed, in RPM
200
250
350
400
0°
OK
15°
OK
30°
OK
45°
OK
Not recommended
60°
OK
Not recommended
75°
OK
Not recommended
1
Data for samples, at 37°C and 240 RPM
2
Recommended interval between prolonged operations –not less than 8 hours
3
Only with the stacking kit installed. Base side-mounted leg parts are required for third level stacking
4
Accurate within ±10%

Orbital shaker-incubator ES-20/80 Edition 1.04 –December of 2021
Operating manual Page 13
8. Ordering information
8.1. Models and versions available:
Model
Version
Description
ES-20/80
V.1GD
230 V, 50/60 Hz, no platforms
8.2. To inquire about or order the optional accessories, contact Grant or your local Grant representative.
8.3. Optional accessories for ES-20/80:
Model
Description
HSP-30/100
Platform with clamps for 30 flasks of 100 ml
HSP-16/250
Platform with clamps for 16 flasks of 250 ml
HSP-9/500
Platform with clamps for 9 flasks of 500 ml
HSP-6/1000
Platform with clamps for 6 flasks of 1000 ml
PP-400
Flat platform with non-slip silicone mat. Working dimensions 360x400 mm
UP-168
Universal platform for different flasks
HSC-50
Clamp for 50 ml flask for UP-168 platform (ø 50 mm)
HSC-100
Clamp for 100 ml flask for UP-168 platform (ø 65 mm)
HSC-250
Clamp for 250 ml flask for UP-168 platform (ø 85 mm)
HSC-500
Clamp for 500 ml flask for UP-168 platform (ø 105 mm)
HSC-1000
Clamp for 1000 ml flask for UP-168 platform (ø 130 mm)
TR-21/50
Variable angle test tube rack for 21 tubes of 50 ml for UP-168 platform
TR-44/15
Variable angle test tube rack for 44 tubes of 15 ml for UP-168 platform
Bluetooth adapter
Stacking kit for two units
Stacking kit for three units

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Page 14 Operating manual
9. Guarantee and service
9.1. Guarantee. When used in laboratory conditions and according to these working instructions, this
product is guaranteed for TWO YEARS (excluding items mentioned in tables in Error! Reference
source not found.) against faulty materials or workmanship.
9.2. Service. There are no user-serviceable parts inside theunit. For all maintenance and repairs (except
as defined below) return to our service department in the UK or in other countries, our distributor.
9.3. Cleaning and disinfection.
9.3.1. Cleaning the outside parts. Use mild soap and water with a soft cloth or sponge for cleaning the
exterior. Rinse remaining washing solution with distilled water. Wipe dry the excess water with clean,
soft cloth or sponge.
9.3.2. Disinfecting the exterior plastic and metal parts. Use 75% ethanol or DNA/RNA removing solution
(e.g., PDS-250). After disinfecting it is necessary to wipe the surfaces dry.
9.4. Fuse replacement. Disconnect the power cable from the mains. Disconnect
the power cable from the socket on the rear of the unit. Open the fuse holder,
located near the socket. Check and replace with a correct fuse if necessary,
see table below:
Model & version
Fuse1
ES-20/80 V.1GD (230 V)
M 4 A
1
Fuse type M - time lag Medium

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10. Compliance
EU Declaration of Conformity
All the products covered by this Manual comply with the requirements of the EU harmonised legisla-
tion verified using the following standards
Low Voltage Directive (2014/35/EC) for Electrical safety.
LVS EN 61010 Part 1
LVS EN 61010 Part 2-010
LVS EN 61010 Part 2-051
EMC directive (2014/30/EC) for Electromagnetic compatibility
LVS EN 61326-1
RoHS Directive (Directive 2011/65/EC including 2015/863)
for Hazardous substances
LVS EN 50581
UK Declaration of Conformity
All the products covered by this Manual comply with the requirements of UK statutory requirements
verified using the following standards.
Electrical Equipment (Safety) Regulations 2016
BS EN 61010 Part 1
BS EN 61010 Part 2-010
BS EN 61010 Part 2-051
Electromagnetic Compatibility Regulations 2016
BS EN 61326-1
The Restriction of the Use of Certain Substances in Electrical and
Electronic equipment Regulations 2012
BS EN 50581
Waste Electrical and Electronic Equipment (WEEE)
All the products covered by this Manual are marked with the crossed-out wheelie bin sym-
bol indicating they must not be disposed of with unsorted waste. Safe recycling of WEEE
helps conserve natural resources and protect human health.
Grant Instruments complies fully with the UK Waste Electrical & Electronic Equipment
(WEEE) regulations 2013. We are a member of the B2B compliance scheme (Scheme
Approval Number WEE/MP3338PT/SCH), which handle our WEEE obligations on our be-
half. Grant Instruments have been issued with a unique registration number by the Environmental
Agency, this reference number is WEE/GA0048TZ.
For information regarding WEEE collections in the UK please contact our B2B Compliance Scheme
directly on 01691 676 124 or www.b2bcompliance.org.uk
In the EU, Grant Instruments complies with WEEE Directive 2012/19/EU.
Contact your local equipment supplier for WEEE collections.
REACH Regulations
This product does not contain any Substances of Very High Concern (SVHCs) at greater than 0.1%
that have to be identified in accordance with Regulation (EC) No 1907/2006 and therefore does not
have an entry in the SCIP database.

Grant Instruments
(Cambridge) Ltd
Shepreth
Cambridgeshire
SG8 6GB
UK
Tel: [phone]
Email: [e-mail]
www.grantinstruments.com
Representative in the European Union
Grant Instruments Europe B.V
Strawinskylaan 411
WTC, Tower A, 4th Floor
1077 XX Amsterdam
The Netherlands
Email: [e-mail]
Orbital shaker-incubator / ES-20/80 / 1.04