Grant-bio LMC-3000 USA User manual

Operating instructions
Laboratory centrifuge
LMC-3000 USA
-bio

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011

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Centrifuge LMC-3000 USA
Operating Instructions
Contents
Version 6 - December 2011
1 Safety .................................................................................4
2 General Information ..........................................................6
3 Getting started...................................................................7
4 Operation ..........................................................................9
5 Specifications ..................................................................11
6Maintenance ....................................................................13
7 Declaration of Conformity ..............................................15

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Centrifuge LMC-3000 USA
Operating Instructions
The following symbol means:
Caution: Read these operating instructions fully before use and pay particular attention to
sections containing this symbol
GENERAL SAFETY
Use only as specified in the operating instructions provided.
The unit should be saved from shocks or drops.
The unit must be stored and transported in a horizontal position (see package label).
After transport or storage allow the unit to dry out (2-3 hrs) before connecting to the mains.
It is necessary to observe the safety area of 300 mm around the centrifuge in accordance with EN-61010-2-20.
Persons and hazardous materials must not be located in the safety area whilst the centrifuge is in operation.
Use only original accessories (rotors, adaptors, etc.) provided by the manufacturer and ordered specifically for
this model.
ELECTRICAL SAFETY
Connect only to the mains with a voltage corresponding to that on the serial number label.
Ensure that the switch and plug are easily accessible during use.
Do not plug the unit into the main outlet without grounding, and do not use extension lead without grounding.
Before moving the unit, disconnect it from the mains. To turn off the unit, disconnect the power plug from the
mains outlet.
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt on or inside
the equipment. If liquid is spilt inside the unit, disconnect it from the mains and have it checked by a competent
person.
DURING OPERATION
Do not centrifuge flammable or chemically vigorously reactive materials. If such liquids are spilled in the rotor or
rotor chamber the centrifuge must be cleaned with a most cloth and a mild soap solution.
Do not use rotors with visible signs of corrosion, wear or mechanical damage.
Do not fill in the containers after they are inserted in the rotor.
1. Safety
Version 6 - December 2011

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Centrifuge LMC-3000 USA
Operating Instructions
Do not leave the operating unit unattended.
Do not operate the unit in environments with aggressive or explosive chemical mixtures.
Do not operate the unit if it is faulty or been incorrectly installed.
For indoor use only.
Do not use outside laboratory rooms.
Before using any cleaning or decontamination method except those recommended by the manufacturer, check
with the manufacturer that the proposed method will not damage the equipment.
Do not make modifications to the design of the unit.
BIOLOGICAL SAFETY
Without bioseal the centrifuge is not a biosafety system in accordance to EN61010-2-20 and cannot be used for
centrifuging hazardous materials contaminated with toxic, radioactive or pathogenic micro-organisms.
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt on or inside
the equipment.
Version 6 - December 2011

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Centrifuge LMC-3000 USA
Operating Instructions
Fig.1 Overall view
Rotor
Power switch
Control
panel
Fuse
compartment
Power
socket
Opening for external
speed control
2. General Information
LMC-3000 USA is a modern benchtop laboratory centrifuge useful for sedimentation of
cells, bacteria, yeast, formed blood elements. It provides operation with tubes and
microtest plates. LMC-3000 USA is designed for safe work (metal protecting housing),
easy maintenance and wide application range in biochemical, industrial and other type
laboratories.
Version 6 - December 2011

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Centrifuge LMC-3000 USA
Operating Instructions
3. Getting started
3.1.Unpacking
Remove packing materials carefully, and retain for future shipment or storage of the unit.
3.2. The Laboratory Centrifuge LMC-3000 USA standard set includes:
• Laboratory Centrifuge LMC-3000 USA .................................................1 pce.
• A spare fuse (inside the fuse holder) ....................................................1 pce.
• Power cord ...........................................................................................1 pce.
• Wrench for rotor replacement ...............................................................1 pce.
• Operating instructions, Certificate ........................................................1 pce.
Optional accessories:
• Rotor R-6 ...............................................................................................on request
• Rotor R-12/10 ........................................................................................on request
• Rotor R-12/15 ........................................................................................on request
• Rotor R-2 ...............................................................................................on request
• Rotor support stand RR-U ......................................................................on request
• Adapter sets BN-13/75, BN-13/100 and BN-16/100 for R-12/15 .............on request
3.3.Set up:
• place the centrifuge on the even stable and clean surface;
• plug the mains power cord into the socket on the rear, and position the centrifuge so that
there is easy access to the power switch and connector;
• it is necessary to observe the safety area of 300 mm around the centrifuge in accordance
with EN-61010-2-20. Persons and hazardous materials must not be located in the safety
area whilst the centrifuge is in operation. This area should be marked.
• do not place any objects in front of the ventilation slots underneath and 100 mm behind the
centrifuge.
Version 6 - December 2011

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011
3.4. Rotor replacement
• Hold the rotor with one hand and with the help of the supplied wrench (13 mm) turn
fixation nut (fig.2/1) counter-clockwise to release the rotor.
• Replace the rotor and secure the new rotor carefully and turning the fixation nut tightly.
1
Fig.2 Rotor replacement

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011
Fig.3 Control panel
124
3
5
10:00 2000
0000
STOP
USA
4. Operation
Recommendations during operation
When loading use even number of tubes arranged symmetrically (facing one another) to give the
unit even balance during operation. The opposite tubes must be filled up equally.
·Centrifuge containers must not be filled over the capacity specified by the manufacturer.
·Rotor must always be fixed securely. Stop the operation immediately with the RUN/STOP key if
any unusual noise occurs during acceleration which can be due to improper rotor fixation.
4.1. Check the power cord for any signs of damage and replace if necessary. Connect the power cord
to the mains outlet . Set the power switch in
position I (ON) on the rear side.
4.2. The centrifuge turns on and lid opens automatically.
The display shows the following readouts:
·previously set time and speed in the upper line (Set);
·mode indication (OPEN - lid opend, rotor stopped) and current speed - 0 RPM in the
lower line (Actual).
4.3. Check the rotor and buckets for any signs of wear or corrosion and replace if necessary.
Insert EVEN number of tubes/microtest plates in rotor facing one another. The loading in
the opposite tubes must be equal.
which provides a safety earth (ground) terminal

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011
4.4. Close the lid (the clicking sound of the lock) readings STOP and (fig.3/5) in the lower
line of the display indicate that the lid is closed.
Note! If any of these readings has not appeared on the display
centrifugation cannot be started. Try to open and close the lid again.
4.5. With the “p” and “q” TIME buttons (fig. 3/1) set the required time interval (0-90 min,
increment 1 min).
4.6. With the “p” and “q” RPM buttons (fig. 3/2) set the required speed (100-3000 RPM,
increment 100 RPM). Note that the speed can also be adjusted during operation.
Note that some plastic tubes and microtest plates can be damaged at higher speeds.
Refer to the tube material specifications to make sure that it will not get damaged at the
set speed. Do not set speed higher than 2000 RPM when working with microtest plates!
4.7. Press RUN/STOP button (fig. 3/3) to start centrifugation. Blinking indication RUN and
current speed is displayed in the lower line. The timer in the upper line starts countdown
after the set speed is achieved (stable indication RUN).
Note! If the rotor imbalance occurs causing vibration the centrifuge stops automatically
(indication IMBALANCE). After the rotor is stopped open the lid and remedy the cause of
imbalance.
4.8. Centrifugation is stopped automatically after the set time elapses while display shows
blinking indication STOP. A sound signal is emitted after full stop of the rotor (press
RUN/STOP button (fig.3/3) to stop the signal).
4.9. If necessary centrifugation can be stopped before the set time elapses by pressing
RUN/STOP button. The set time interval will be shown on the display.
4.10. Press the button open (fig.3/4) and open the lid lifting it upwards with a hand (it is
possbile to open the lid only when the rotor is stopped). Display shows OPEN.
4.11. At the end of operation set the power switch in position O (OFF) on the rear.
4.12. Disconnect the power cord from the mains outlet.
Note! The electrical lid lock allows opening the lid only when the unit is connected to the
mains and is turned on. Do not force the lid to open when the unit is switched off!
Lid emergency opening
-Disconnect the power cord from the mains outlet and allow the centrifugation to stop.
-Slide the unit to the front of the bench to access the emergency opening slot on the underside of
the unit (located in the front side).
-Avoid tilting the unit as this may cause spilling of the materials from the containers inside the
unit.
-Insert a small screwdriver (or similar tool with diameter up to 3 mm) into the emergency opening
slot in front of the dot on the label “Open” at a depth of 10-15 mm.
-Move the lever from the left to the right hand side to release the lid lock.
(STOP and )

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011
5. Specifications
•
• Rotation direction ..........................................................................counter-clockwise;
• Timer ..........................................................................1 - 90 min (increment - 1 min);
• Rotor imbalance automatic diagnostics (emergency stop, indication “IMBALANCE”);
• Rotor slowdown time, not more .......................................................................30 sec;
• Display.................................................................................................................LCD;
• Chamber working diameter ..........................................................................335 mm;
• ;
• Dimensions .................................................................................. 495x410x235 mm;
• Weight, not more ...........................................................................................13,5 kg;
Speed range .................................................100 - 3000 RPM (increment 100 RPM);
Operating voltage ....................................................... 120 V, 50/60 Hz, 120 W (1 A)
• Operating conditions
The unit is designed for operation in cold rooms, incubators and closed laboratory
rooms at ambient temperature from +4°C to +40°C and maximum relative humidity
80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011
Grant is committed to a continuous programme of improvement, specifications may be
changed without notice.

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011
6. Maintenance
6.1.Guarantee
When used in laboratory conditions and according to these working instructions, this
product is guaranteed for TWO YEARS against faulty
materials or workmanship.
6.2.Service & Maintenance
There are no user-serviceable parts inside the unit. For all maintenance and repairs
(exept as defined below) return to our service department in the UK or in other
countries,our distributor.
6.3.Cleaning & Disinfection
Standard ethanol (75%) or other cleaning agents recommended for cleaning of laboratory
equipment can be used for cleaning and disinfection of the unit.
It is recommended to perform disinfection after operation session by cleaning the parts
inside the centrifuge chamber. Rotors and other accessories are not autoclavable.
6.4.Replacement of fuses (fig.4)
Disconnect from the outlet.
Remove the power plug from the rear of the unit. Pull out the
fuse holder by applying leverage in recess (A). Remove the
fuse from the holder. Check and replace with the correct fuse if
necessary (for 230V, T1A or for 120V, T2A).
6.5.Routine safety tests
If routine tests are to be made, we recommend a test of the integrity of the protective
earth conductor and an insulation test at 500 Vdc. Routine flash tests are not
recommended for any electrical equipment, because repeated high voltage tests
degrade insulation materials.
6.6. Aditional items
BN-16/90 standard adapter set for R-12/10
BN-17/120 standard adapter set for R-12/15
BN-13/75 adapter set for R-12/15
BN-13/100 adapter set for R-12/15
BN-16/100 adapter set for R-12/15
Rotor holder RR-U
(exclude items mentioned in p.6.6)
Fig.4 Fuse holder
A

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011

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Centrifuge LMC-3000 USA
Operating Instructions
Version 6 - December 2011

Grant Instruments
(Cambridge) Ltd
Shepreth,
Cambridgeshire
SG8 6GB
Tel: [phone]
www.grant.co.uk
[email protected]
Fax: [phone]
Centrifuge/LMC-3000/28713/6