Grant-bio PCV-2400 User manual

Operating instructions
Centrifuge/Vortexer
Combi-spin
PCV-2400
For versions: V.3GA
V.3GC
V.3G3

1 Safety .............................................................................................3
2 General Information......................................................................4
3 Getting started ..............................................................................5
4 Operation of PCV-2400 .................................................................6
5 Specifications................................................................................8
6 Maintenance ..................................................................................9
7 Guarantee and service ...............................................................10
8Declaration of Conformity..........................................................11
Contents
Page 3
Combi-spin PCV-2400
Operating Instructions Version 1.03 - July 2013

1.1 Caution symbol
The following symbol means:
Caution! Make sure you have fully read and understood the present operating instructions
before using the equipment. Please pay special attention to sections marked by
this symbol.
1.2 Safety features
The PCV-2400 is constructed so as to meet the requirements of international safety standard IEC
61010-2-020: Safety requirements for electrical equipment for measurement, control, and
laboratory use – Part 2-020: Particular requirements for laboratory centrifuges, and national
standards based on it, including:
EN 61010-2-020;
BS EN 61010-2-020;
A copy of the Declaration of Conformity with CE requirements is included at the back of this manual.
Particular safety features include:
УA microswitch that prevents the centrifuge motor from being started while the lid is open;
УAn interlock that prevents the lid from being opened until the rotor has slowed down.
1.3. Safety precautions
GENERAL SAFETY
Use only as specified in the Operating Instructions provided.
The unit should not be used if dropped or damaged.
After transportation or storage keep the unit under room temperature for 2–3 hrs before
connecting it to the electric circuit.
Use only cleaning and decontamination methods recommended by the manufacturer.
Do not make modifications to the design of the unit.
1. Safety
Page 4 Centrifuge/Vortexer
Operating Instructions
PCV-2400
Version 1.03 - July 2013

ELECTRICAL SAFETY
Connect only to electric circuit with voltage corresponding to that on the serial number label.
Do not plug the unit into an ungrounded power socket, and do not use an ungrounded extension
lead.
Ensure that the plug is easily accessible during use.
Disconnect the unit from the electric circuit before moving.
Disconnect the power cord plug from the power socket to turn off the unit.
If liquid penetrates into the unit, disconnect it from the electric circuit and have it checked by a
repair and maintenance technician.
DURING OPERATION
Do not open the lid during work, run-up or run-down of the rotor.
Do not operate the unit in environments with aggressive or explosive chemical mixtures.
Do not use rotors with visible signs of corrosion, wear or mechanical damage.
Do not operate the unit if it is faulty or has been installed incorrectly.
Do not use outside laboratory rooms.
BIOLOGICAL SAFETY
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt
on or penetrates into the equipment.
Page 5
Combi-spin PCV-2400
Operating Instructions Version 1.03 - July 2013

2. General Information
The Centrifuge/Vortexer Combi-spin PCV-2400 is specially designed for life science research. It
can be used in biochemical, microbiological and industrial biotechnology laboratories.
The PCV-2400 makes it possible to centrifuge a batch of samples and at the same time mix other
samples using the concave cup, which is driven by the same drive shaft as the centrifuge.
The PCV-2400 is designed for handling small quantities of liquid. Examples include micro quantity
enzyme reactions; mixing in microtubes prior to incubation, and in dry block heaters and coolers.
It can help to save working space in laminar flow or PCR boxes.
Page 6 Centrifuge/Vortexer
Operating Instructions
PCV-2400
Version 1.03 - July 2013

3. Getting started
3.1. Unpacking
Remove packing materials carefully and retain them for future shipment or storage of the unit.
Examine the unit carefully for any damage incurred during transit. The warranty does not cover in-
transit damage.
3.2. PCV-2400 standard set includes:
·Centrifuge/Vortexer PCV-2400 ...........................................................................1 pce.
·Rotor R-05-02 12 x 0.5ml + 12 x 0.2ml u..............................................................1 pce.
·Rotor R-15 12 x 1.5ml v......................................................................................1 pce.
·Concave cup 18090 z........................................................................................1 pce.
·Spare Fuse .........................................................................................................1 pce.
·Operating instructions, Declaration of conformity ...............................................1 copy
Optional accessories:
·rotor PR2-05 8 x 1.5/2.0ml + 8 x 0.5ml w.......................................................on request
·rotor PR2-05-02 6 x 1.5/2.0ml + 6 x 0.5ml + 6 x 0.2ml x................................on request
·rotor PSR-16 2 x 8 well strips or 0.2ml y.......................................................on request
3.3. Set up:
- place the unit on an horizontally even working surface;
- position the unit so that there is easy access to the plug.
-according to EN 61010-2-20 people and hazardous materials must not be within a 300 mm area
around the device during the centrifuge operation.
3.4. Rotor replacement
The unit is provided with the fixation mechanism for all the types of the above mentioned rotors; The
operator can easily change the rotor for the desired type of tubes in w–y s. To change a rotor hold it
with one hand and turn the concave cup (fig.1/ ) counterclockwise to set rotor free. Change the
rotor and re-attach the concave cup by turning until secure.
Page 7
Combi-spin PCV-2400
Operating Instructions Version 1.03 - July 2013

4. Operation of PCV-2400
Page 8 Centrifuge/Vortexer
Operating Instructions
PCV-2400
Recommendation during operation
Check the rotor for any signs of wear and replace if necessary. Insert EVEN number of tubes
in rotor one opposite another. The opposite tubes must be filled equally.
4.1. Connect unit to a properly grounded power socket.
4.2. Operation in the quick centrifugation mode:
4.2.1 Turn the switch (Fig.1/x) into position I (QS, Quick Spin).
Fig.1 General view
4 TUBE
ARRANGEMENT
2 TUBE
ARRANGEMENT 6 TUBE ARRANGEMENT FULL ARRANGEMENT
Version 1.03 - July 2013

Page 9
4.2.2 Open the lid and place EVEN number of tubes to the rotor sockets (Fig.1/v)
opposite one another. Close the lid.
4.2.3 Press the QS (Fig.1/w) button for quick mixing / spinning and hold it pressed for
the desired time. The unit will automatically stop when the QS button is released.
Note! Do not open the lid whilst the rotor is moving. The unit stops automatically when the
lid is opened.
4.2.4 Turn the switch (Fig. 1/x) into position O (OFF).
4.3. Operation in the vortex mode:
4.3.1 Turn the switch (Fig. 1/x) into position I (QS).
4.3.2 Gently holding a tube with fingers at its upper part press the tube's lower part to the
vortex head cavity bottom (Fig.1/u).
4.3.3 Press QS button for mixing and hold it pressed until full sediment dissolution.
Caution! Please note that the mixing effectiveness is dependent on the angle of the tube
and the pressure applied.
To avoid premature wear of the vortex head and plastic tube's surfaces don't press
tubes to the vortex head with too much force.
4.3.4 Turn the switch (Fig. 1/x) into position O (OFF).
4.4. Operation in the long centrifugation mode:
4.4.1 Open the lid and place EVEN number of tubes to rotor sockets (Fig.1/v) opposite
one another. Close the lid.
4.4.2 Turn the switch (Fig. 1/x) into position II (LS, Lon Spin) to start centrifugation.
4.4.3 Turn the switch into position O (OFF) to stop centrifugation.
4.5. Disconnect unit from the electric circuit.
Combi-spin PCV-2400
Operating Instructions Version 1.03 - July 2013

5. Specifications
Page 10 Centrifuge/Vortexer
Operating Instructions
PCV-2400
The product is designed for operation indoors in a laboratory at altitudes up to 2000 m, with
ambient temperature from +4°C to +40°C and maximum relative humidity 80% for temperatures up
to 31°C decreasing linearly to 50% relative humidity at 40°C.
·
Fixed rotation speed .............................................................................2800 rpm (50 Hz)
3500 rpm (60 Hz)
·
Acceleration time to max. speed ..............................................................................5 sec
·
Maximum continuous operation time..................................................................... 60 min
·
Dimensions ..........................................................................................190х235х125 mm
·
Nominal operating voltage/ Consumed power ...................230 V; 50 Hz; 30 W (0.13 A),
·230 V; 60 Hz; 25 W (0.1 A) or 120 V; 50/60 Hz; 30 W (0.27 A)
·
Weight*....................................................................................................................1.7 kg
* Accurate within ±10%.
Grant is committed to a continuous programme of improvement, specifications may be changed
without notice.
DescriptionReplacement parts
Description
Optional accessories
rotor for 12 x 1.5 ml microtest tubesR-15
rotor for 12 x 0.5 + 12 x 0.2 ml microtest tubesR-05-02
rotor for 8 x 2.0 ml + 8 x 0.5 ml microtest tubesPR2-05
rotor for 6x2,0 ml + 6x0.5 ml + 6x0.2 ml
microtest tubes
PR2-05-02
rotor for 2 strips 8 x 0,2 ml microtubesPSR16
concave cup with 2 mm eccentric 18090
Version 1.03 - July 2013

6. Maintenance
6.1 Cleaning and Disinfection
Clean the centrifuge with a damp cloth, using water only. In particular ensure that the sucker
feet are kept clean. Do not use chemical cleaning agents. Standard ethanol (75%) can be used
for disinfection. Before using any other cleaning or decontamination method, check with the
manufacturer or supplier to make sure that the proposed method will not damage the
equipment.
6.2 Routine maintenance and inspections
There are no user-serviceable parts inside the unit. For all maintenance and repairs (except as
defined below) return to our service department in the UK or in other countries, our distributor.
6.1.1 Maintenance
Inspect the spin plate and vortex head for mechanical damage/wear. Worn or damaged
parts should not be used and must be replaced. Replacement spin plates and vortex
heads can be obtained from Grant, but wear/damage to the centrifuge drive shaft will
necessitate its return to Grant, or its agent, for repair.
6.1.2 Routine Safety checks
If routine tests are to be made, we recommend a test of the integrity of the protective
earth conductor and an insulation test at 500 Vdc. Routine flash tests are not
recommended for any electrical equipment, because repeated high voltage tests
degrade insulation materials.
Values for tests:
Earth continuity 0.1 Ohm max.
Insulation resistance 10 M Ohm min.
6.1.3 Fuse replacement
Disconnect the unit from the mains power supply. Open the fuse holder located on rear
side of the unit by turning its cover counter-clockwise. Replace with the correct fuse.
Fuse ratings
240 V centrifuge..................20mm x 5mm, F250 mA (250V~)
110 V centrifuge..................20mm x 5mm, F500 mA (250V~)
Page 11
Combi-spin PCV-2400
Operating Instructions Version 1.03 - July 2013

7. Guarantee and Service
7.1 Guarantee
When used in laboratory conditions and in accordance with these working instructions,
this centrifuge is guaranteed for TWO YEARS against faulty materials or workmanship.
7.2 Service
Equipment requiring repair should be sent to our Service Department in the UK or in
other countries to our distributor.
Page 12Centrifuge/Vortexer
Operating Instructions
PCV-2400
Version 1.03 - July 2013

Page 13
Manufacturer:
Equipment name/type number:
Description of Equipment:
Directive:
Harmonized Standards:
Applied Standards
Combi-spin PCV-2400
Operating Instructions Version 1.03 - July 2013

Page 14Centrifuge/Vortexer
Operating Instructions
PCV-2400
Version 1.03 - July 2013

Centrifuge-Vortexer Combi-spin /PCV-2400/17994/1.03
Grant Instruments
(Cambridge) Ltd
Shepreth
Cambridgeshire
SG8 6GB
UK
Tel: [phone]
Fax: [phone]
Email: [email protected]
www.grantinstruments.com