Grant-bio PHMP User manual

Operating instructions
Thermo shaker
PHMP/ PHMP-4
For version V.2GW

Page 2 Thermo shaker PHMP/PHMP-4
Operating instructions
Version 2.03 - July 2013
Contents
1 Safety..........................................................................................3
2 General Information ..................................................................5
3 Getting Started ..........................................................................6
4 Operation of PHMP/PHMP-4.....................................................7
5 Specifications............................................................................9
6 Guarantee and service............................................................10

Page 3
Thermo shaker PHMP/PHMP-4
Operating instructions
1. Safety
The following symbols mean:
Caution! Make sure you have fully read and understood the present manual before
using the equipment. Please pay special attention to sections marked by
this symbol.
Caution! Surfaces can become hot during use.
GENERAL SAFETY
Use only as specified in the operating manual provided.
The unit should not be used if dropped or damaged.
The unit must be stored and transported in a horizontal position (see package label).
After transportation or storage keep the unit at room temperature for 2–3 hrs before
connecting it to the electric circuit.
Use only cleaning and decontamination methods recommended by the manufacturer.
Do not make modifications to the design of the unit.
ELECTRICAL SAFETY
Connect only to the external power supply unit with voltage corresponding to that on the
serial number label.
Use only the external power supply unit provided with this product.
Ensure that the switch and external power supply unit are easily accessible during use.
Do not plug the unit into an ungrounded power socket, and do not use an ungrounded
extension lead.
Disconnect the unit from the electric circuit before moving.
Disconnect the external power supply unit from power socket to turn off the unit.
If liquid penetrates into the unit, disconnect it from the external power supply unit and
have it checked by a repair and maintenance technician.
Version 2.03 - July 2013

Page 4 Thermo shaker PHMP/PHMP-4
Operating instructions
DURING OPERATION
Do not leave the operating unit unattended.
Do not impede the platform motion.
Do not operate the unit in environments with aggressive or explosive chemical mixtures.
Do not operate the unit if it is faulty or has been installed incorrectly.
Do not use outside laboratory rooms.
Do not check the temperature by touch. Use a thermometer.
BIOLOGICAL SAFETY
It is the user's responsibility to carry out appropriate decontamination if hazardous
material is spilt on or penetrates into the equipment.
Version 2.03 - July 2013

2. General Information
The Thermo Shaker PHMP/PHMP-4 is designed for shaking a that holds
1–4 standard microplates, and controlling the set temperature in the range of 25°C to
60°C (if the room temperature (RT) is less than 25°C).
The main features of theThermo-Shaker are
u Additional heating source, in the lid of the device, which allows:
• faster heat-up times;
• increase of temperature range;
•
v Compact size.
w Informative LCD, which allows instant control over both set and current temperatures
of the aluminium block, as well as the shaking intensity and duration.
The Thermo Shaker was designed using the multi-system principle, which allows use as
three independent devices:
u Incubator – for incubation without shaking of microplates;
v Microplate Shaker – for operation in the cold room or other conditions, which do not
require temperature stabilisation;
w Thermo Shaker – for immunochemistry and molecular diagnostics, where stringent
requirements of repeatability and precision are necessary.
An external 12 V power supply unit is used to power the unit. This makes it safe for use
in the cold room, where condensation may cause leakage current from the electric
circuit. The device can be used in:
• cytochemistry – for in situ reactions;
• immunochemistry – for immunofermentative reactions;
• biochemistry – for enzyme and protein analysis;
• molecular biology – for matrix analysis.
The maximum guaranteed number of diagnostic cycles in the Thermo-Shaker mode,
which require 15–30 min work in one cycle, is 7000–14000 times.
special platform
reduces condensation in the plate.
Thermo shaker PHMP/PHMP-4
Operating instructions Page 5
Version 2.03 - July 2013

3. Getting started
3.1 Unpacking
Remove packing materials carefully and retain them for future shipment or storage of
the unit.
Examine the unit carefully for any damage incurred during transit. The warranty does not
cover in-transit damage.
3.2 Thermo-Shaker set includes:
• Thermo Shaker PHMP/PHMP-4 .........................................................1 piece
• Spare rubber drive belt......................................................................2 pieces
• External power supply unit..................................................................1 piece
• Operating instructions; Declaration of Conformity ...............................1 copy
3.3 Set up
Place the unit onto an even horizontal non-flammable surface away from any flammable
materials (not less than 30 cm);
Plug the external power supply unit into the socket at the rear side of the PHMP and
position the unit so that there is easy access to the power switch and the external power
supply unit.
Page 6 Thermo shaker PHMP/PHMP-4
Operating instructions
Version 2.03 - July 2013

4. Operation of PHMP / -4PHMP
4.1 Connect the external power supply unit to a grounded power socket and set the power switch
located on the rear panel of the unit to position I ("ON").
4.2 The display will turn on with the upper line (Set) showing time, speed and temperature set
earlier and the lower line (Actual) showing current readings of the same parameters
(thermoblock temperature °C, which automatically starts rising according to the temperature
set in the upper line). The time of temperature stabilisation depends on the initial temperature.
Setting the parameters
Use the readings in the upper line of the display (Set), while setting the necessary parameters.
Setting time (TIME)
4.3 Using the p and q keys (Fig. 1/u) set the required working time interval in hours and minutes
(increment - 1 min). Pressing the key for more than 3 sec will increase the increment.
Setting speed (RPM)
4.4 Using the p and q keys (Fig. 1/v) set the required speed (increment 10 RPM). Pressing the
key for more than 3 sec will increase the increment.
Setting temperature (T,ºC)
4.5 Using the p and q keys (Fig. 1/w) set the necessary temperature (increment 0.1ºC).
Pressing the key for more than 3 sec will increase the increment.
Caution! The platform heating can be turned off only by setting the required temperature
below 25°C (the display will show OFF – T,ºC – set point). It can be used in cold
rooms as a mixing device without thermal regulation in this mode.
Program execution
After the thermal stabilisation of the unit (when the set and current temperature readings
become the same)
Page 7
Thermo shaker PHMP/PHMP-4
Operating instructions
Set
Actual
hr : min
Thermo-Shaker
00:00 1000 35.0
STOP 0000 35.0
Fig. 1 Control panel
Version 2.03 - July 2013

4.6 Microplate fixation:
PHMP: Place microplates on the platform and fix it with the special push-down clip by
pressing it against the plate covers.
PHMP-4: Unscrew the fixation screws. Place microplates on the platform and fix them by
tightening the fixation screws.
Caution! For model PHMP: Load only pairs of microplates for best fixing.
Caution! For model PHMP-4: The microplate fixation screws must always be tightened to
avoid damage. Tighten the fixation screws completely when microplates are
removed from or placed on the platform. Do not close the lid if the microplate fixation
screws are not tightened to avoid damage.
4.7 Press the RPM-RUN/STOP key (Fig. 1/x). The platform will start rotating and the timer
indicator will start counting up the time interval (with 1 min precision).
4.8 After finishing the program the platform motion will stop and the timer will show the flashing
reading STOP accompanied by the repetitive sound signal until the RPM-RUN/STOP key is
pressed.
4.9 If the working time is not set (or is reset) and the timer indicator in the upper line shows 00:00,
pressing the RPM-RUN/STOP key will start continuous operation of the Thermo-shaker
(timer indicator will start counting up the time interval in the lower line (Actual)) until the RPM-
RUN/STOP key is pressed again.
Please note! The platform temperature will be constantly maintained in accordance with the
set temperature. This allows the devise to be used again without pre-heating.
4.10 The timer can be reset during operation if required. Press the TIME-RUN/STOP key once
(Fig. 1/y) to stop the timer. Press the TIME-RUN/STOP key again to restart the timer.
4.11 The platform motion can be stopped at any time by pressing the RPM-RUN /STOP key. In this
case the program realisation run and the platform motion will stop and the timer will switch into
the STOP mode saving previously set time. Press the RPM-RUN/STOP key to repeat the
operation with the same time and speed.
Caution! At the end of the set time period the platform movement is stopped automatically,
but the heating can be stopped only by reducing the temperature using the q T(ºC)
key (Fig. 1/w lower key) till the OFF sign appears in the upper part of the display.
4.12 After finishing the operation set the power switch, located on the rear panel of the unit, in
position O (Off) and disconnect the external power supply from electric circuit.
Page 8 Thermo shaker PHMP/PHMP-4
Operating instructions
Correct loading Wrong loading
PHMP PHMP-4
Version 2.03 - July 2013

5. Specifications
5.1 Thermo Shaker PHMP/PHMP-4 provides:
·soft or intensive sample shaking;
·rotation speed control, stabilisation and display;
·even shaking amplitude throughout the Thermo shaker platform;
·required operation time setting and display;
·automatic stopping of the platform movement after the set time expires;
·current operation time display;
·setting and display of the required temperature.
5.2 Temperature specifications
·Temperature setting range ......................................................+25°C ... +60°C (increment 0,1°C)
·Temperature control range........................................................................5°C above RT to +60°C
·Temperature stability............................................................................................................±0.1°C
·Temperature accuracy .........................................................................................................±0.5°C
·Temperature uniformity at +37ºC.......................................................................................±0.25°C
·Time of block heating from +25°C to +37ºC ........................................................................12 min
5.3 General specifications
·Speed setting range ................................................................250–1200 rpm (increment 10 rpm)
·Orbit.......................................................................................................................................2 mm
·Display..................................................................................................................16x2 signs, LCD
·Digital time setting range ........................................................................1 min – 96 hrs / non-stop
·Max. continuous operation time............................................................................................96 hrs
(recommended interval between operation sessions not less than 8 hrs)
·Time setting resolution...........................................................................................................1 min
·Max. height of miocroplate...................................................................................................18 mm
·External power supply............................................ input AC 100-240 V 50/60Hz, output DC 12V
PHMP PHMP-4
·Current/power consumption 12 V DC, 3.3 A / 40 W 12 V DC, 4.15 A / 50 W
• Platform loading capacity 24
• Platform plate dimensions 250x150 mm 210x290 mm
• Dimensions 270x260x125 mm 380x390x140 mm
• Weight*6.1 kg 8.8 kg
•The product is designed for operation indoors in a laboratory at altitudes up to 2000 m, with ambient
temperature from +4°C to +40°C and maximum relative humidity 80% for temperatures up to 31°C
decreasing linearly to 50% relative humidity at 40°C.
* Accurate within ±10%.
Grant is committed to a continuous programme of improvement, specifications may be changed without
notice.
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Thermo shaker PHMP/PHMP-4
Operating instructions Version 2.03 - July 2013

6. Guarantee and Service
6.1 Guarantee
When used in laboratory conditions and according to these working instructions, this
product is guaranteed for TWO YEARS against faulty materials or workmanship. For full
Details of the Grant Bio Warranty policy please contact Grant Instruments.
6.2 Service
For service, return for repair to our Service Department in the UK or, in other countries,
to our distributor.
6.2.1 Replacing Drive belt
For the maintenance of reliable operation of the device Grant recommends to
replace the rubber belts after 1.5 years or 2000 hours of operation time.
1. Disconnect the external power supply unit
from the device.
2. Remove 4 fixation screws on the device
base and remove the bottom plate.
3. Replace the rubber drive belt (fig. 2).
4. Re-assemble the device.
6.2.2 Spare parts:
- Rubber drive belt (117x5x0.6 mm);
- External power supply unit (part no. 18020)
input AC 100–240 V, 50–60 Hz; output DC 12 V.
6.3 Cleaning & disinfection
Standard ethanol (75%) or other cleaning agents recommended for cleaning of
laboratory equipment can be used for cleaning and disinfection of the unit.
Page 10 Thermo shaker PHMP/PHMP-4
Operating instructions
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Fig. 2 Drive belt
Version 2.03 - July 2013

Page 11
Thermo shaker PHMP/PHMP-4
Operating instructions
Manufacturer:
Equipment name/type number:
Description of Equipment:
Directive:
Harmonized Standards:
Applied Standards
Version 2.03 - July 2013

Thermo shaker/PHMP/PHMP-4/18004/2.03
Grant Instruments
(Cambridge) Ltd
Shepreth
Cambridgeshire
SG8 6GB
UK
Tel: [phone]
Fax: [phone]
Email: [email protected]
www.grantinstruments.com