Grant-bio LMC-3000 USA User manual

Operating instructions
Laboratory centrifuge
LMC-3000
For versions V.3GD
V.3GE

Contents
Page 2
Laboratory centrifuge LMC-3000
Operating Instructions
1 Safety .................................................................................3
2 General Information ..........................................................5
3 Getting started...................................................................6
4 Operation of Centrifuge....................................................8
5 Specifications..................................................................10
6 Guarantee and service....................................................12
7 Declaration of Conformity ..............................................13
Version 3.01 - October 2012

The following symbols mean:
Caution: Read these operating instructions fully before use and pay particular attention to
sections containing this symbol
GENERAL SAFETY
Use only as specified in the operating instructions provided.
The unit should not be used if it has encountered a physical shock or has been dropped.
The unit must be stored and transported in a horizontal position (see package label).
After transport or storage allow the unit to dry out (2-3 hrs) before connecting to the mains.
It is necessary to observe the safety area of 300 mm around the centrifuge in accordance with EN-
61010-2-20. Persons and hazardous materials must not be located in the safety area whilst the
centrifuge is in operation.
Use only original accessories (rotors, adaptors, etc.) provided by the manufacturer and ordered
specifically for this model.
ELECTRICAL SAFETY
Connect only to the mains with a voltage corresponding to that on the serial number label.
Ensure that the switch and plug are easily accessible during use.
Do not plug the unit into the main outlet without grounding, and do not use extension lead without
grounding.
Before moving the unit, disconnect it from the mains. To turn off the unit, disconnect the power plug
from the mains outlet.
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt on
or inside the equipment. If liquid is split inside the unit, disconnect it from the mains and have it
checked by a competent person.
DURING OPERATION
Do not centrifuge flammable or chemically vigorously reactive materials. If such liquids are spilled in
the rotor or rotor chamber the centrifuge must be cleaned with a most cloth and a mild soap solution.
1. Safety
Page 3
Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

Page 4
Do not use rotors with visible signs of corrosion, wear or mechanical damage.
Do not fill in the containers after they are inserted in the rotor.
Do not leave the operating unit unattended.
Do not operate the unit in environments with aggressive or explosive chemical mixtures.
Do not operate the unit if it is faulty or been incorrectly installed.
For indoor use only.
Do not use outside laboratory rooms.
Before using any cleaning or decontamination method except those recommended by the manufacturer,
check with the manufacturer that the proposed method will not damage the equipment.
Do not make modifications to the design of the unit.
BIOLOGICAL SAFETY
Without bioseal the centrifuge is not a biosafety system in accordance to EN61010-2-20 and cannot be
used for centrifuging hazardous materials contaminated with toxic, radioactive or pathogenic micro-
organisms.
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt on or
inside the equipment.
Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

2. General Information
LMC-3000 is a modern benchtop laboratory centrifuge useful for sedimentation of cells,
bacteria, yeast and blood samples. It spins tubes and microtest plates. LMC-3000 is
designed for safe work (metal protecting housing), easy maintenance and wide
application range in medical, biochemical, industrial and other types of laboratories.
FEATURES:
• Easy set up of centrifugation parameters (time and speed) and simultaneous display
of the set and actual parameter values.
• Safety: metal protective housing and metal lid, automatic imbalance switch-off, lid
lock when centrifuge is running provide safe operation at all speeds.
• Rotor imbalance automatic diagnostics (emergency stop, indication “IMBALANCE”).
• Low noise level.
• Soft run-up and run-down of the rotation.
• Wide choice of accessory rotors.
Centrifugation process causes moderate heating of operating chamber, which does not
exceed 15°С above room temperature during an hour and a half. In cases where quickly
inactivating samples are to be centrifuged the preliminary cooling or use of refrigerated
centrifuge LMC-4200R is recommended.
Page 5
Fig.1 Overall view
Rotor
Power switch
Control
panel
Fuse
compartment
Power
socket
Opening for external
speed control
Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

3. Getting started
3.1.Unpacking
Remove packaging carefully, and retain for future shipment or storage of the unit.
3.2. The Laboratory Centrifuge LMC-3000 set includes:
Standard set
Laboratory centrifuge LMC-3000 ...........................................................................1 pce.
A spare fuse (inside the fuse holder) .....................................................................1 pce.
Power cord .............................................................................................................1 pce.
Wrench for rotor replacement .................................................................................1 pce.
Operating instructions, Declaration of Conformity .................................................1 copy
Optional accessories
Rotor R-6 u.....................................................................................................on request
Rotor R-12-10 v..............................................................................................on request
Rotor R-12-15 w..............................................................................................on request
Rotor R-2 x.....................................................................................................on request
Rotor support stand RR-U y...........................................................................on request
Adapter sets BN-13-75, BN-13-100 and BN-16-100 for R-12-10 z................on request
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Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

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3.3. Set up:
·place the unit on an even stable and clean surface;
·plug the power cord into the socket on the rear, and position the unit so that there is
easy access to the power switch and mains;
·it is necessary to observe the safety area of 300 mm around the centrifuge in
accordance with EN-61010-2-20. Persons and hazardous materials must not be
located in the safety area whilst the centrifuge is in operation;
·it is necessary to observe the safety area of at least 100 mm from the rear of the
centrifuge.
3.4. Rotor replacement
Caution! Check the rotor and adapters for any signs of wear or corrosion and replace if
necessary.
·Hold the rotor with one hand and with the help of the supplied wrench (13 mm) turn
fixation nut (fig.2/ ) counter-clockwise to release the rotor.
Caution! Do not hold the rotor by rings or adapters mounting when mounting and fixing
it. Hold the rotor as shown on Fig. 2 (Correct).
·Replace the rotor and secure the new rotor carefully and turning the fixation nut tightly.
Laboratory centrifuge LMC-3000
Operating Instructions
Fig.2 Rotor fixation
Correct Wrong
Version 3.01 - October 2012

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4. Operation of LMC-3000
Recommendations during operation
·When loading use even number of tubes arranged symmetrically (facing one another) to
give the unit even balance during operation. The opposite tubes must be filled up
equally.
·Centrifuge containers must not be filled over the capacity specified by the manufacturer.
Rotor must always be fixed securely. Stop the operation immediately with the RUN/STOP
key if any unusual noise occurs during acceleration which can be due to improper rotor
fixation.
4.1. Check the power cord for any signs of damage and replace if necessary. Connect the
power cord to the mains outlet which provides a safety earth (ground) terminal. Set the
power switch in position I (ON) on the rear side.
4.2. The centrifuge turns on and lid opens automatically.
The display shows the following readouts:
·previously set time and speed in the upper line (Set);
·mode indication (OPEN - lid opend, rotor stopped) and current speed - 0 RPM in the
lower line (Actual).
4.3. Check the rotor and buckets for any signs of wear or corrosion and replace if necessary.
Insert EVEN number of tubes/microtest plates in rotor facing one another. The opposite
tubes must be filled up equally.
4.4. Carefully close the lid (listen for the clicking sound of the lock) readings STOP and
(fig.3/5) in the lower line of the display indicate that the lid is closed.
Note! If any of these readings (STOP and ) has not appeared on the display
centrifugation cannot be started. Try opening and closing the lid again.
4.5. With the “p” and “q” TIME buttons (fig. 3/ ) set the required time interval (0-90 min,
increment 1 min).
4.6. With the “p” and “q” RPM buttons (fig. 3/ ) set the required speed (100-3000 RPM,
increment 100 RPM). Note that the speed can also be adjusted during operation.
Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

Page 9
Note: Some plastic tubes and microtest plates can be damaged at higher speeds. Refer
to the tube material specifications to make sure that it will not get damaged at the set
speed. Do not set speed higher than 2000 RPM when working with
microtest plates!
4.7. Press RUN/STOP button (fig. 3/ ) to start centrifugation. Blinking indication RUN and
current speed is displayed in the lower line. The timer in the upper line starts countdown
after the set speed is achieved (stable indication RUN).
Note: If the rotor imbalance occurs causing vibration, the centrifuge stops automatically
(indication IMBALANCE). After the rotor is stopped, open the lid and remedy the cause
of imbalance.
4.8. Centrifugation is stopped automatically after the set time elapses while display shows
blinking indication STOP. A sound signal is emitted after full stop of the rotor (press
RUN/STOP button (fig.3/ ) to stop the signal).
4.9. If necessary centrifugation can be stopped before the set time elapses by pressing
RUN/STOP button. The set time interval will be shown on the display.
4.10. Press the button Open (fig.3/ ) and open the lid lifting it upwards with a hand (it is
possbile to open the lid only when the rotor is stopped). Display shows OPEN.
4.11. At the end of operation set the Power switch in position O (OFF) on the rear.
4.12. Disconnect the power cord from the mains outlet.
Note: The electrical lid lock allows opening the lid only when the unit is connected to the
mains and is turned on. Do not force the lid to open when the unit is switched off!
Lid emergency opening
·Disconnect the power cord from the mains outlet and allow the centrifugation to stop.
·Slide the unit to the front of the bench to access the emergency opening slot on the
underside of the unit (located in the front side).
·Avoid tilting the unit as this may cause spilling of the materials from the containers
inside the unit.
·Insert a small screwdriver (or similar tool with diameter up to 3 mm) into the
emergency opening slot in front of the dot on the label “Open” at a depth of 10-15 mm.
·Move the lever from the left to the right hand side to release the lid lock.
Fig.3 Control panel
10:00 2000
0000
STOP
Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

Page 10
5. Specifications
The product is designed for operation indoors in a laboratory at altitudes up to 2000 m, with
ambient temperature from +4°C to +40°C and maximum relative humidity 80% for
temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.
·Speed setting range .....................................100 - 3000 RPM (increment 100 RPM)
100 - 2000 RPM using the rotor R-2
·Digital time setting .......................................................1 - 90 min (increment - 1 min)
·Acceleration time up max. speed, not more.....................................................35 sec
·Slowdown time, not more.................................................................................35 sec
·Rotor imbalance automatic diagnostics (emergency stop, indication “IMBALANCE”)
·Rotation direction ...........................................................................counterclockwise
·Display.................................................................................................................LCD
·Maximum noise level, not more.........................................................................65 dB
·Working diameter ..........................................................................................335 mm
·Dimensions .................................................................................. 495x410x235 mm
·Operating voltage/ power consumption ........................ 230 V, 50 Hz / 110 W (0.5 A)
or 120 V, 50/60 Hz / 120 W (1 A)
·Weight, not more ...............................................................................................13 kg
Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

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Grant is committed to a continuous programme of improvement, specifications may be changed
without notice.
Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

6. Guarantee and service
6.1.Guarantee
When used in laboratory conditions and according to these working instructions, this
product is guaranteed for TWO YEARS (exclude items mentioned in Table below) against
faulty materials or workmanship.
BN-16-90 standard adapter set for R-12-10
BN-17-120 standard adapter set for R-12-15
BN-13-75 adapter set for R-12-10
BN-13-100 adapter set for R-12-10
BN-16-100 adapter set for R-12-10
Rotor holder RR-U
6.2.Service & Maintenance
There are no user-serviceable parts inside the unit. For all maintenance and repairs (exept
as defined below) return to our service department in the UK or in other countries, our
distributor.
6.3.Cleaning & Disinfection
Cleaning liquids that do not contain concentrate organic solvents, alkali or acid can be used
for device cleaning.
Rotors and other accessories are not
autoclavable.
Standard ethanol (75%) or other cleaning agents recommended for cleaning of laboratory
equipment can be used for cleaning and disinfection of the unit.
6.4.Replacement of fuses (fig.4)
6.5.Routine safety tests
If routine tests are to be made, we recommend a test of the integrity of the protective earth
conductor and an insulation test at 500 Vdc. Routine flash tests are not recommended for
any electrical equipment, because repeated high voltage tests degrade insulation materials.
Aditional items
It is recommended to perform disinfection after operation session by
cleaning the parts inside the centrifuge chamber.
Disconnect from the outlet.
Remove the power plug from the rear of the unit. Pull out
the fuse holder by applying leverage in recess (A).
Remove the fuse from the holder. Check and replace with
the correct fuse if necessary (for 230V - T1A or for 120V -
T2A).
Fig.4 Fuse holder
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Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

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Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

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Laboratory centrifuge LMC-3000
Operating Instructions
Version 3.01 - October 2012

Laboratory centrifuge/LMC-3000/28713/3.01
Grant Instruments
(Cambridge) Ltd
Shepreth
Cambridgeshire
SG8 6GB
UK
Tel: [phone]
Fax: [phone]
Email: [email protected]
www.grantinstruments.com