HACH LANGE LCK 303 User guide

DOC027.98.00789
POCKET COLORIMETERTM II -
Ammonium/Amonio/Ammoniaca
LCK 303/304/305
APPLICATION INSTRUCTION
PROGRAMMIERANLEITUNG
INSTRUCTIONS DE FONCTIONNEMENT
INSTRUCCIONES DE APLICACIÓN
ISTRUZIONI APPLICATIVE
GEBRUIKSAANWIJZING
INSTRUKTIONSHANDBOK
10/2010, Edition 2A
10/2010, Ausgabe 2A
10/2010, édition 2A
10/2010, Edición 2A
10/2010, edizione 2A
10/2010, Editie 2A
10/2010, Utgåva 2A
© HACH LANGE, 2010. All rights reserved. Printed in Germany

2

3
Table of contents
Section 1 General information .......................................................................5
1.1 Safety information .......................................................................................5
1.1.1 Use of hazard information ................................................................5
1.2 General product information .......................................................................5
Section 2 Installation ........................................................................................7
2.1 Packing list ..................................................................................................7
2.2 Insert the adapter ........................................................................................7
2.3 Replace the instrument cap ........................................................................7
Section 3 System start up ...............................................................................9
3.1 Instrument keys and display .......................................................................9
3.1.1 Key-Functions ...................................................................................9
3.2 Enter calibration data of HACH-LANGE Cuvette Tests ..............................9
3.2.1 Overview ...........................................................................................9
3.2.2 Data table .......................................................................................10
3.3 Programming procedure ...........................................................................11
3.3.1 LCK 303 Ammonium-Nitrogen NH4-N ............................................11
3.3.2 LCK 304 Ammonium-Nitrogen NH4-N ............................................12
3.3.3 LCK 305 Ammonium-Nitrogen NH4-N ............................................13
3.4 Check entered data ..................................................................................14
3.5 Overwrite stored calibration data ..............................................................15
3.5.1 Retrieve the factory calibration .......................................................15
Section 4 Operation ........................................................................................17
4.1 Analyse Ammonium-Nitrogen/Ammonium
with HACH-LANGE Cuvette Tests .......................................................17
4.2 Quality assurance .....................................................................................17
4.3 Evaluate HACH-LANGE Ammonium-Nitrogen/
Ammonium Cuvette Tests ...................................................................17
Section 5 Replacement Parts and Accessories ....................................19
5.1 Replacement Parts ...................................................................................19
5.2 Accessories ..............................................................................................19
Section 6 How to order ..................................................................................21

Table of contents
4

5
Section 1 General information
1.1 Safety information
Please read this entire application instruction and the POCKET COLORIMETERTM II
instruction manual before unpacking, setting up, or operating this equipment. Pay
attention to all danger and caution statements. Failure to do so could result in serious
injury to the operator or damage to the equipment.
To ensure that the protection provided by this equipment is not impaired, do not use
or install this equipment in any manner other than that specified in this manual.
1.1.1 Use of hazard information
DANGER
Indicates a potentially or imminently hazardous situation which, if not
avoided, could result in death or serious injury.
Important Note: Information that requires special emphasis.
Note: Information that supplements points in the main text.
1.2 General product information
This application instruction describes how to evaluate HACH-LANGE Cuvette Tests
on a single-wavelength POCKET COLORIMETER II.

General information
6

7
Section 2 Installation
Important Note: This application instruction is only valid in conjunction with the
POCKET COLORIMETERTM II instruction manual and the applied LCK working
procedure. Please read this entire application instruction and the entire POCKET
COLORIMETER II instruction manual before unpacking, setting up or operating the
instrument.
2.1 Packing list
•POCKET COLORIMETER II 655 nm, Cat. No. 5870065
•Instrument cap with HACH-LANGE label, Cat. No. LZV 759
•Cuvette adapter 1 cm, Cat. No. 5954600
•Blank value cuvettes, Cat. No. LCW 919
•Application instruction DOC027.98.00789
2.2 Insert the adapter
Remove the instrument cap and carefully position the cuvette adapter into the cell
holder with the arrow pointing downward. Fix it by pressing it down.
2.3 Replace the instrument cap
Use the instrument cap with the HACH-LANGE label when evaluating HACH-LANGE
cuvettes.

Installation
8

9
Section 3 System start up
3.1 Instrument keys and display
3.1.1 Key-Functions
ZERO/SCROLL (BLUE key) : In measurement mode, sets the instrument to zero. In
menu mode, scrolls through menu options. Also scrolls numbers when entering or
editing a value.
READ/ENTER (GREEN key) : In measurement mode, initializes sample
measurement. In menu mode, selects a menu option. When entering numbers,
moves one space to the right. Confirm the function when the entry is complete.
3.2 Enter calibration data of HACH-LANGE Cuvette
Tests
3.2.1 Overview
Each POCKET COLORIMETER II has two channels in which measurements can be
made. Each channel (measuring range) will accept user-defined calibration data.
Figure 1 Instrument keys and display
1 POWER/BACKLIGHT 6 Measuring range indicator (2)
2 ZERO/SCROLL (BLUE key)7Menu indicator
3MENU 8Calibration adjusted indicator
4Numeric display 9Battery low indicator
5Measuring range indicator (1) 10 READ/ENTER (GREEN key)
1 absorbance 21absorbance 2
655 nm

System start up
10
This application instruction contains the calibration data of the HACH-LANGE
Cuvette Tests for Ammonium-Nitrogen/Ammonium:
•LCK 303: 2-47 mg/l NH4-N or 2.5-60 mg/l NH4
•LCK 304: 0.015-2 mg/l NH4-N or 0.02-2.5 mg/l NH4
•LCK 305: 1-12 mg/l NH4-N or 1.2-15 mg/l NH4
Select the calibration data of maximum two measuring ranges which will be the
suitable one.
Each programming procedure has only to be followed once prior to the first test
evaluation.
For detailed information please read the POCKET COLORIMETER II instruction
manual.
3.2.2 Data table
3.2.2.1 Evaluation as Ammonium-Nitrogen NH4-N
3.2.2.2 Evaluation as Ammonium NH4
Table 1 Evaluation as Ammonium-Nitrogen NH4-N
LCK 303 LCK 304 LCK 305
RES (resolution) --.-- -.--- --.--
S0 (standard 0) 02.00 0.015 00.99
A0 (absorbance 0) 0.113 0.044 0.203
S1 (standard 1) 47.00 2.000 12.00
A1 (absorbance 1) 1.862 1.832 1.871
Table 2 Evaluation as Ammonium NH4
LCK 303 LCK 304 LCK 305
RES (resolution) --.-- -.--- --.--
S0 (standard 0) 02.50 0.020 01.30
A0 (absorbance 0) 0.110 0.047 0.198
S1 (standard 1) 60.00 2.500 15.00
A1 (absorbance 1) 1.845 1.783 1.788

System start up
11
3.3 Programming procedure
Note: Each programming procedure has only to be followed once prior to the first test
evaluation.
Important Note: The following programming procedures are valid for the evaluation
form Ammonium-Nitrogen NH4-N. To evaluate as Ammonium NH4please choose
the data mentioned in table 3.2.2.2 Evaluation as Ammonium NH4 on page 10.
Note: After programming the calibration data, it could be helpful to mark the channel(s) 1
and/or 2 accordingly with a label.
3.3.1 LCK 303 Ammonium-Nitrogen NH4-N
1. Press POWER to switch on the instrument.
2. Press MENU. "SEL" is displayed. A flashing arrow indicates the current
measuring range (1 or 2).
3. Select measuring range 1 or 2 for LCK 303 (2-47 mg/l NH4-N).
Note: Press GREEN key to toggle between measuring ranges.
4. Press MENU for about five seconds until "USER" followed by "CAL" is
displayed.
5. Press BLUE key until "Edit" is displayed. Press GREEN key. " RES" is
displayed.
6. Press GREEN key. "-.---" is displayed.
7. Press BLUE key until "--.--" is displayed. Press GREEN key. "S0" is displayed.
8. Press GREEN key until "A0" is displayed.
9. Press GREEN key until "S1" is displayed.
10. Press GREEN key. Enter "47.00" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "A1" is
displayed.
11. Press GREEN key. Enter "1.862" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "Add" is
displayed.
12. Press MENU until "Edit" is displayed. Press GREEN key until „S0“ is displayed.
13. Press GREEN key. Enter "02.00" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "A0" is
displayed.
14. Press GREEN key. Enter "0.113" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until „S1“ is
displayed.
15. Press MENU until "Edit" is displayed to check the entered data.
Press GREEN key. „RES“ is displayed.

System start up
12
Press BLUE key to scroll through the entries. Compare the entries with the data
shown in the table below.
16. After comparing the entries press MENU two times to enter the measuring
mode. See 4.3 Evaluate HACH-LANGE Ammonium-Nitrogen/ Ammonium
Cuvette Tests on page 17.
Note: In case of any mistake retrieve the factory calibration first. See 3.5.1 Retrieve the factory
calibration on page 15. After retrieving the factory calibration, start the programming procedure
again. If the instrument is shut-off or loses power during data entry, all edits will be lost.
Automatic shut-off is 60 minutes.
3.3.2 LCK 304 Ammonium-Nitrogen NH4-N
1. Press POWER to switch on the instrument.
2. Press MENU. "SEL" is displayed. A flashing arrow indicates the current
measuring range (1 or 2).
3. Select measuring range 1 or 2 for LCK 304 (0.015-2 mg/l NH4-N).
Note: Press GREEN key to toggle between measuring ranges.
4. Press MENU for about five seconds until "USER" followed by "CAL" is
displayed.
5. Press BLUE key until "Edit" is displayed. Press GREEN key. " RES" is
displayed.
6. Press GREEN key. "-.---" is displayed.
7. Press GREEN key. "S0" is displayed.
8. Press GREEN key until "A0" is displayed.
9. Press GREEN key until "S1" is displayed.
10. Press GREEN key. Enter "2.000" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "A1" is
displayed.
11. Press GREEN key. Enter "1.832" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "Add" is
displayed.
12. Press MENU until "Edit" is displayed. Press GREEN key until „S0“ is displayed.
13. Press GREEN key. Enter "0.015" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "A0" is
displayed.
Table 3 Calibration data of LCK 303
RES --.--
S0 2.00
A0 0.113
S1 47.00
A1 1.862

System start up
13
14. Press GREEN key. Enter "0.044" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until „S1“ is
displayed.
15. Press MENU until "Edit" is displayed to check the entered data.
Press GREEN key. „RES“ is displayed.
Press BLUE key to scroll through the entries. Compare the entries with the data
shown in the table below.
16. After comparing the entries press MENU two times to enter the measuring
mode. See 4.3 Evaluate HACH-LANGE Ammonium-Nitrogen/ Ammonium
Cuvette Tests on page 17.
Note: In case of any mistake retrieve the factory calibration first. See 3.5.1 Retrieve the factory
calibration on page 15. After retrieving the factory calibration, start the programming procedure
again. If the instrument is shut-off or loses power during data entry, all edits will be lost.
Automatic shut-off is 60 minutes.
3.3.3 LCK 305 Ammonium-Nitrogen NH4-N
1. Press POWER to switch on the instrument.
2. Press MENU. "SEL" is displayed. A flashing arrow indicates the current
measuring range (1 or 2).
3. Select measuring range 1 or 2 for LCK 305 (1-12 mg/l NH4-N).
Note: Press GREEN key to toggle between measuring ranges.
4. Press MENU for about five seconds until "USER" followed by "CAL" is
displayed.
5. Press BLUE key until "Edit" is displayed. Press GREEN key. " RES" is
displayed.
6. Press GREEN key. "-.---" is displayed.
7. Press BLUE key until "--.--" is displayed. Press GREEN key until "S0" is
displayed.
8. Press GREEN key until "A0" is displayed.
9. Press GREEN key until "S1" is displayed.
10. Press GREEN key. Enter "12.00" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "A1" is
displayed.
11. Press GREEN key. Enter "1.871" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "Add" is
displayed.
Table 4 Calibration data of LCK 304
RES -.---
S0 0.015
A0 0.044
S1 2.000
A1 1.832

System start up
14
12. Press MENU until "Edit" is displayed. Press GREEN key until „S0“ is displayed.
13. Press GREEN key. Enter "00.99" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until "A0" is
displayed.
14. Press GREEN key. Enter "0.203" by using BLUE key to change value and
GREEN key to move to the next space. Press GREEN key until „S1“ is
displayed.
15. Press MENU until "Edit" is displayed to check the entered data.
Press GREEN key. „RES“ is displayed.
Press BLUE key to scroll through the entries. Compare the entries with the data
shown in the table below.
16. After comparing the entries press MENU two times to enter the measuring
mode. See 4.3 Evaluate HACH-LANGE Ammonium-Nitrogen/ Ammonium
Cuvette Tests on page 17.
Note: In case of any mistake retrieve the factory calibration first. See 3.5.1 Retrieve the factory
calibration on page 15. After retrieving the factory calibration, start the programming procedure
again. If the instrument is shut-off or loses power during data entry, all edits will be lost.
Automatic shut-off is 60 minutes.
3.4 Check entered data
To avoid erroneous measurements it is recommended to check the data after
completing the programme procedure.
1. Press MENU for about five seconds until "USER" followed by "CAL" is
displayed.
2. Press BLUE key to scroll to "Edit". Press GREEN key.
3. "RES" is displayed. Press BLUE key to scroll through the entries. Compare the
entries with the figures in the 3.2.2.1 Evaluation as Ammonium-Nitrogen NH4-N
on page 10.
Incasea value wasnot stored correctly, retrieve the factory calibration first. See 3.5.1
Retrieve the factory calibration on page 15. After retrieving the factory calibration,
start the programming procedure again.
Table 5 Calibration data of LCK 305
RES --.--
S0 0.99
A0 0.203
S1 12.00
A1 1.871

System start up
15
3.5 Overwrite stored calibration data
With the POCKET COLORIMETER II it is possible to overwrite stored calibration
data. Before overwriting calibration data it is recommended to retrieve the factory
calibration first. After retrieving the factory calibration, you can program new
calibration data.
In case of any mistake retrieve the factory calibration first.
3.5.1 Retrieve the factory calibration
1. Press MENU. "SEL" is displayed. A flashing arrow indicates the current
measuring range (1 or 2). Press GREEN key to toggle between measuring
ranges. Select measuring range retrieving the factory calibration.
1. Press MENU for about five seconds until "USER" followed by "CAL" is
displayed.
2. Press BLUE key to scroll to "dFL". Press GREEN key.

System start up
16

17
Section 4 Operation
4.1 Analyse Ammonium-Nitrogen/Ammonium with
HACH-LANGE Cuvette Tests
DANGER
Handling chemical samples, standards and reagents can be dangerous. Users
of this product are advised to familiarize themselves with safety procedures
and the correct use of chemicals, and to carefully read all relevant material
safety data sheets.
To analyse Ammonium or Ammonium-Nitrogen follow the working procedure
enclosed in the according Cuvette Test LCK 303, LCK 304 or LCK 305.
4.2 Quality assurance
The measurement results must be subjected to plausibility checks (dilute and/or
spike the sample).
Regular checks with corresponding standard solutions (see Section 5 Replacement
Parts and Accessories on page 19) ensure that your measurement system is
functioning properly and is being correctly operated.
4.3 Evaluate HACH-LANGE Ammonium-Nitrogen/
Ammonium Cuvette Tests
Note: Make sure that the instrument is programmed with the corresponding calibration data,
see 3.4 Check entered data on page 14.
1. Fill DI water into an empty cuvette (LCW 919) up to the lower edge of the
barcode label.
2. Close the cuvette with the rubber stopper. This is the blank cuvette.
3. Thoroughly clean the outside of the cuvette.
4. Press POWER to switch on the instrument.
5. Make sure that the correct measuring range is selected!
Otherwise press MENU. "SEL" is displayed. A flashing arrow indicates the
current measuring range (1 or 2). Press GREEN key to select measuring range.
Press MENU to get back to the measuring mode.
6. Insert the blank cuvette into the cell holder equipped with the cuvette adapter
1cm.
Note: Make sure that the cuvette adapter is positioned correctly.
7. Place the instrument cap with the HACH-LANGE label over the cell holder to
cover the cuvette.
8. Press BLUE key. "0.00" (LCK 303/305) or "0.000“ (LCK 304) is displayed.
9. Remove the instrument cap and insert the sample cuvette.
10. Place the instrument cap over the cell holder to cover the cuvette.
11. Press GREEN key.

Operation
18
12. The result is displayed in mg/l Ammonium-Nitrogen/Ammonium.
Note: In case the Ammonium-Nitrogen/Ammonium concentration is out of the selected
measuring range the value for the lowest or highest concentration is displayed and
flashes. Repeat the test using the appropriate LCK Cuvette Test measuring range or
dilute your sample.
Note: A new blank measurement should be performed prior to each individual test series.

19
Section 5 Replacement Parts and
Accessories
5.1 Replacement Parts
Description Quantity Cat. Number
POCKET COLORIMETERTM II 655 nm 15870065
Instrument cap with HACH-LANGE label 1 LZV 759
Cuvette adapter 1 cm 15954600
Blank value cuvettes 1 pk/5 pc LCW 919
5.2 Accessories
Description Quantity Cat. Number
Ammonium Cuvette Test (2-47 mg/l NH4-N) 1 pk/25 pc LCK 303
Ammonium Cuvette Test (0.015-2 mg/l NH4-N) 1 pk/25 pc LCK 304
Ammonium Cuvette Test (1-12 mg/l NH4-N) 1 pk/25 pc LCK 305
Standard and Ring Test solution for LCK 304 1 LCA 700
Standard and Ring Test solution for LCK 303 1LCA 703
Standard and Ring Test solution for LCK 305 1 LCA 704
Pipette 0.2-1 ml 1BBP 078
Pipette tips for BBP 078 1 pk/100 pc BBP 079
Pipette 1-5 ml 1BBP 065
Pipette tips for BBP 065 1 pk/75 pc BBP 068
Cuvette Stand for HACH-LANGE cuvettes 1LYW 915

Replacement Parts and Accessories
20