NEW BRUNSWICK SCIENTIFIC MP-1000 Pourmatic User manual

MP-1000 Pourmatic
Automated Plate Pourer
MANUAL No. M1224-0050
Revision Q
September 22, 2005
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: [phone] •1-800-631-5417
Fax: [phone] •Telex: 4753012 NBSCO
Internet: http://www.nbsc.com •E-mail: [email protected]

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MP-1000 PourMatic M1224-0050 User’s Guide

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New Brunswick Scientific Co., Inc. User’s Guide
INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
CHINA
New Brunswick Scientific Co., Inc.
Room 1501, Xiangjiang Building, No. 18
Lane 1265, Zhongshan Road (W)
Shanghai 200051, P.R. China
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
FRANCE
New Brunswick Scientific SARL
3, rue des Deux-Boules
75001 Paris
France
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: [phone] or 0800 581331
Fax: [phone]
E-mail: [email protected]
Web: www.nbsuk.co.uk

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MP-1000 PourMatic M1224-0050 User’s Guide
CAUTION!
This equipment must be operated as described in this manual. If
operational guidelines are not followed, equipment damage and
personal injury can occur.
Please read the entire User’s Guide before attempting to use this
unit.
CAUTION!
For optimum operation of this unit, please restrict your use of petri
dishes to the following only:
60 x 15mm Falcon 1007
Fisher 8-757-13A
100 x 15mm Falcon 1029
Fisher 8-757-13
Lab-Tek 4002

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New Brunswick Scientific Co., Inc. User’s Guide
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
©Copyright 2005 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and
improvement.
Manual Conventions
Bold Text in bold face type emphasizes key words or
phrases.
#NOTE:
CAUTION!
WARNING!
Caution messages appear before procedures
which, if caution is not observed, could result
in damage to the equipment.
Notes contain essential information that
deserves special attention.
Warning messages alert you to specific
procedures or practices which, if not followed
correctly, could result in serious personal
injury.

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MP-1000 PourMatic M1224-0050 User’s Guide
Every Instrument manufactured by the New Brunswick Scientific
Co., Inc. is warranted to be free from defects in material and work-
manship. This apparatus with the exception of glassware, lamps and
electrodes (where supplied), is warranted for 1 year against faulty com-
ponents and assembly and our obligation under this warranty is limited
to repairing or replacing the instrument or part thereof, which shall
with-in 1 year after date of shipment, prove to be defective after our
examination. This warranty does not extend to any NBS products
which have been subjected to misuse, neglect, accident or improper
installation or application; nor shall it extend to products which
have been repaired or altered outside the NBS factory
without prior authorization from the
New Brunswick Scientific Co., Inc.
W A R R A N T Y

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New Brunswick Scientific Co., Inc. User’s Guide
TABLE OF CONTENTS
1OVERVIEW.........................................................................................................................9
1.1 SPECIFICATIONS............................................................................................................10
1.2 CE DECLARATION OF CONFORMITY .............................................................................10
2INSPECTION & VERIFICATION .................................................................................12
2.1 INSPECTION OF BOXES ..................................................................................................12
2.2 PACKING LIST VERIFICATION .......................................................................................12
3PREPARING THE LOCATION......................................................................................13
3.1 PHYSICAL LOCATION ....................................................................................................13
3.2 ENVIRONMENT..............................................................................................................13
4UNPACKING & INSPECTION.......................................................................................14
4.1 UNPACKING OF EQUIPMENT..........................................................................................14
4.2 INSPECTION OF EQUIPMENT ..........................................................................................15
5FEATURES........................................................................................................................17
6SETTING UP .....................................................................................................................22
6.1 LEVELING .....................................................................................................................22
6.2 INSTALL SPILL TUBE.....................................................................................................23
6.3 CAROUSEL ATTACHMENT .............................................................................................23
6.4 INSERTING THE CATCH TRAY........................................................................................24
7GETTING STARTED.......................................................................................................25
7.1 VOLTAGE CONFIGURATION...........................................................................................25
7.2 MAKING ELECTRICAL CONNECTION .............................................................................25
7.3 INDEXING......................................................................................................................25
7.4 CAROUSEL LOADING FOR CONTINUOUS AND BATCH MODES .......................................26
7.5 CONTINUOUS MODE......................................................................................................26
7.6 BATCH MODE ...............................................................................................................27
7.7 AUTO / MANUAL KEY...................................................................................................29
7.8 NOZZLE ASSEMBLY & STERILIZATION .........................................................................29
7.9 LOADING TUBING INTO PUMP HEAD.............................................................................30
7.10 INSTALLING THE DUST COVER......................................................................................31
8OPERATION .....................................................................................................................33
8.1 SPEED ...........................................................................................................................33
8.2 COOL TIME ...................................................................................................................33
8.3 PRIMING THE PUMP.......................................................................................................34
8.4 CALIBRATION................................................................................................................35
8.5 ENTERING A DISPENSING VOLUME ...............................................................................36

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MP-1000 PourMatic M1224-0050 User’s Guide
9STARTING THE POURMATIC.....................................................................................38
9.1 PRE-RUN CHECKLIST....................................................................................................38
10 UNLOADING PETRI DISHES....................................................................................39
10.1 UNLOADING DISHES IN BATCH MODE ..........................................................................39
10.2 UNLOADING IN CONTINUOUS MODE.............................................................................40
11 CLEARING JAMS........................................................................................................41
11.1 GENERAL GUIDELINES..................................................................................................41
11.2 CAROUSEL JAMS (FLASHING “ERR” CODE & CAROUSEL KEY) ....................................42
11.3 DISH TRANSFER TABLE JAMS (FLASHING “ERR” CODE & TRANSFER TABLE KEY)......43
11.4 DISH ERRORS (FLASHING CODE <100 IN FUNCTION DISPLAY) ...................................45
11.4.1 Flashing Error Code < 45...................................................................................45
11.4.2 Flashing Error Code 45-75 .................................................................................45
11.4.3 Clearing Error Code 12.......................................................................................45
11.4.4 Clearing Error Code 50.......................................................................................46
11.4.5 Clearing Other Error Code Numbers..................................................................47
11.5 ELEVATOR JAMS (FLASHING “ELE” CODE)...................................................................48
11.6 SENSOR ERRORS (FLASHING ERROR CODE 102 OR 103)...............................................50
12 PREVENTIVE MAINTENANCE................................................................................51
12.1 CLEANING PROCEDURES...............................................................................................51
12.2 FUSE REPLACEMENT.....................................................................................................52
13 TROUBLESHOOTING................................................................................................54
13.1 ERROR MESSAGE TABLE...............................................................................................54
14 REPLACEMENT PARTS & ACCESSORIES...........................................................56
14.1 REPLACEMENT PARTS...................................................................................................56
14.2 ACCESSORIES................................................................................................................56
14.3 CONVERSION KIT..........................................................................................................56
15 DRAWINGS...................................................................................................................60
15.1 CONTROL SCHEMATICS.................................................................................................60
15.2 INDEX OF DRAWINGS ....................................................................................................62
16 INDEX.............................................................................................................................63

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New Brunswick Scientific Co., Inc. User’s Guide
1
1 O OV
VE ER RV VI IE EW W
The NBS Pourmatic Plate Pourer is a single-carousel design petri dish filler that
automatically dispenses sterile agar into covered plastic petri dishes. The space-efficient
design uses the same carousel to hold both empty and filled dishes without the need for
separate empty and filled stacking areas. You can load up to 320 dishes of size 100 x 15
or 60 x 15 mm. An optional Conversion Kit (purchased separately) is available for quick
change of petri dish sizes.
Agar flows through autoclavable silicone tubing and a stainless steel nozzle, designed for
rapid and aseptic insertion at the dispensing station. The PourMatic is equipped with a
transparent dust cover and germicidal lamp located over the filling area. This helps
control dust, microbial contamination and ensure sterility.
The PourMatic has two operating modes: Continuous and Batch. Continuous Mode
allows processing one stack of petri dishes at a time. The user removes stacks of filled
dishes and loads empty dishes on a continuous basis.
In Batch Mode, the user loads up to 320 dishes and the PourMatic automatically fills
them in one single run. The agar filled petri dishes cool by natural convection. A digital
timer set by the user, signals the end of a cooling cycle.
Petri dishes are filled with a user-selected volume of agar by an integral peristaltic
metering pump. Accurate and reproducible dispensing is achieved by regulating pump
revolutions. Dispense volume is independent of variations in line voltage. Agar
dispenses at low velocity to provide a gentle flow into the dish and the volume of the
dispensed agar is adjustable by the user. The PourMatic features three operating speeds to
allow gentle handling of full dishes or maximum processing rates with normally filled
dishes. Petri dishes filled by the PourMatic are level, uniform and bubble-free.
The PourMatic shuts off automatically at the end of the filling cycle, signaled by an
audible tone. Automatic shut-off occurs as audible and visible signals alert the user of an
upside-down, half or jammed dish. As a safety feature, the germicidal lamp is controlled
by an interlock switch; it lights only when the dust cover is properly positioned.

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MP-1000 PourMatic M1224-0050 User’s Guide
1.1Specifications
PourMatic Specifications
Alarms Audible and visual signals.
Auto shutoff at end of filling cycle.
Audible and visual signals when jam occurs.
Carousel Speed Selectable speeds achieve gentle dish transfer
when working with large fill volumes
Dimensions 19¼″W x 24″L x 34½″H
(489mm x 610mm x 876mm)
Dish Sizes / Manufacturer
60 x 15mm
100 x 15mm
Falcon 1007
Fisher 8–757–13A
Falcon 1029
Fisher 8–757–13
Lab–Tek 4002
Dispensing
accuracy
repeatability
volume
±1 ml
within ±0.5 ml
from 4–200 ml
Electrical Requirements
fuse 100/120V–4 Amp Slo-Blo®
220/240V–2 Amp Slo-Blo®
Maximum Dish Load 320 (8 stacks of 40 dishes)
Optic Sensors Detect incomplete or improperly positioned
dishes: automatic shutoff & alarm sounds.
Pouring Rate 15 dishes per minute (based on 15 ml volume)
Pump Peristaltic Metering Pump
Microprocessor-controlled with digital
dispense volume calibration
Sterility See-through Dust Cover and Germicidal Lamp
help eliminate dust and contamination
Weight 80 lbs.
Electrical Requirements 100/120V—50/60 Hz, 480VA
220/240V—50/60 Hz, 480VA
Regulatory Standards ETL
CE
1.2 CE Declaration of Conformity
See the following page.

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New Brunswick Scientific Co., Inc. User’s Guide

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MP-1000 PourMatic M1224-0050 User’s Guide
2
2 I IN
NS SP PE EC CT TI IO ON N &
& V VE
ER RI IF FI IC CA AT TI IO ON N
2.1 Inspection of Boxes
After you have received your order from New Brunswick Scientific, inspect the boxes
carefully for any damage that may have occurred during shipping. Report any damage
immediately to your local NBS Sales Order Department or local NBS distributor.
2.2 Packing List Verification
Verify against your NBS packing list that you have received the correct materials.

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New Brunswick Scientific Co., Inc. User’s Guide
3
3 P PR
RE EP PA AR RI IN NG G T TH HE E L
LO
OC CA AT TI IO ON N
3.1 Physical Location
The surface where you place the PourMatic should be smooth, level and sturdy. Ensure
that there are two or more inches of free space around the PourMatic for proper
operation.
3.2 Environment
The PourMatic operates properly under the following conditions:
• incubated and refrigerated
• operating temperature range 5°C–40°C
• relative humidity up to 80% up to 31°C decreasing linearly to 50% at 40°C
• voltage fluctuation ±10% maximum
#NOTE:
The Environmental Operating Range of 5 to 40°C is the range of
temperatures at which the PourMatic can operate. Successful operation is
subject to the agar remaining liquid throughout the dispensing process.

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MP-1000 PourMatic M1224-0050 User’s Guide
4
4 U UN
NP PA AC CK KI IN NG G &
& I IN
NS SP PE EC CT TI IO ON N
4.1 Unpacking of Equipment
Save all packing materials and User’s Guide. If any part of your order was damaged
during shipping, missing pieces or fails to operate properly, please fill out the Customer
Service Form 6300 and return by fax. You can also call the New Brunswick Scientific
Service Department.
The PourMatic is shipped in two separate boxes:
Box Type Box Weight & Dimensions Box Contents
Carousel 21 lbs.
22″X 33″X 31″CAROUSEL & ACCESSORIES
Base 100 lbs.
32″x 25″x 15″BASE
To properly unpack the PourMatic, follow these instructions:
1. Open the top of the CAROUSEL box.
2. Remove the CAROUSEL and then remove the foam packing spacers from both sides
of the CAROUSEL.
3. Remove the bagged ACCESSORIES from the center of the CAROUSEL and set aside.
4. Open the top of the BASE box.
CAUTION!
The PourMatic Base is heavy. Two people are required to lift or move it.
5. Grab the BASE from the bottom and gently place on a sturdy, level surface.
6. Remove packing spacer from the BASE.

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New Brunswick Scientific Co., Inc. User’s Guide
4.2 Inspection of Equipment
Verify that you have received the following:
PourMatic BASE CAROUSEL SECURING KNOB
installed on PourMatic Base
IEC-320 DETACHABLE POWER CORD
PourMatic CAROUSEL
2 DISPENSING NOZZLES
1 installed on PourMatic
1 loose
TRANSPARENT DUST COVER
installed on PourMatic
GERMICIDAL LAMP
installed on PourMatic

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MP-1000 PourMatic M1224-0050 User’s Guide
PourMatic
User’s Guide
12 FT. SILICONE TUBING
CATCH TRAY
2 HOSE CLAMPS
SPARE FUSE
25 ml. GRADUATED CYLINDER
SPILL TUBE
PourMatic USER’S GUIDE

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New Brunswick Scientific Co., Inc. User’s Guide
5
5 F FE
EA AT TU UR RE ES S
• IEC–320 DETACHABLE POWER CORD Located on the right side panel–supplies
power to the PourMatic
• ON / OFF ROCKER SWITCH Located on the right side panel–
controls power of the PourMatic
• CAROUSEL Removable, rotating bin–dishes are
placed through the spring loaded rods
that keep stacked dishes in position
• CAROUSEL SECURING KNOB Secures the CAROUSEL to the
PourMatic Base
• STACKING PLATES (9) One placed on each stack of 60 mm
(for 60mm units only) plates to ensure smooth operation
• GERMICIDAL LAMP Illuminates DISPENSING NOZZLE area to
help ensures sterility–will not illuminate
when DUST COVER is removed
• DUST COVER U.V. absorbent plastic–fits over
dispensing area to help maintain sterility
• DISPENSING NOZZLE Dispenses agar into petri dishes–
sterilizable
• DISPENSING NOZZLE HOLDER Positions DISPENSING NOZZLE prior to
filling dishes
• SILICONE TUBING Agar runs through tubing from reservoir
to petri dishes
• PERISTALTIC PUMP Roller pump for dispensing agar
• SPILL TUBE Diverts spilled agar to the CATCH TRAY
• CATCH TRAY Catches spilled agar & is removable

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MP-1000 PourMatic M1224-0050 User’s Guide
• DISH SENSOR Three fiber optic sensors detect if
dishes are present and positioned
properly–two sensors are to the right
of the DISH TRANSFER TABLE the other
is under the DISH SENSOR PLATE and
pointed up
• STATIONARY PLATFORM Teflon coated platform–supports stacks
of petri dishes
• DISH TRANSFER TABLE Rotating, removable–three position
moves dishes from SENSOR STATION to
DISPENSING STATION and then to
ELEVATOR STATION
• ELEVATOR STATION Reloads petri dishes from the DISH
TRANSFER TABLE to the CAROUSEL
• FUNCTION KEYS Located on the MEMBRANE KEY PAD
Start / Stop Key Stops the PourMatic; starts and stops
the PUMP in MANUAL and in
CALIBRATE
modes
Select Key Used to select CALIBRATE,DISPENSE
VOLUME, COOL TIME, or SPEED %
parameters
Up Key Increases the Setpoint of a
displayed parameter
Down Key Decreases the Setpoint of a
Displayed parameter
SELECT
START
STOP

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New Brunswick Scientific Co., Inc. User’s Guide
Auto / Manual Key Allows user to select
AUTO or MANUAL mode
When in MANUAL, user operates PUMP
manually by pressing START / STOP
KEY
Continuous/Batch Key Allows user to select CONTINUOUS or
BATCH mode: CONTINUOUS mode fills
one stack of dishes; when complete,
dishes can be removed and replaced with
new dishes without interrupting the filling
cycle. PourMatic stops when a station is
empty or when you press the START /
STOP KEY
BATCH mode allows user to run a
complete cycle: PourMatic stops
automatically and sounds an alarm
alerting user that cycle is complete
Carousel Transfer Key Rotates CAROUSEL–used to index the
CAROUSEL after a jam–LED illuminates
red when jams occur
Dish Transfer Key Rotates DISH TRANSFER TABLE–Used
to index the DISH TRANSFER TABLE
after a jam–LED illuminates red when
jams occur
CONTINUOUS
BATCH
A
UTO
MANUAL

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MP-1000 PourMatic M1224-0050 User’s Guide
• FUNCTION INDICATORS Located on the MEMBRANE KEY PAD
Calibrate User adjustsPUMP
calibration data
Dispense Volume User adjusts the desired amount of agar
that is transferred to the petri dish
Cool Time After a cycle is complete the internal
timer counts down a cool period for the
agar and sounds an alarm when cool time
is complete
Speed % User adjust the speed of the DISH
TRANSFER TABLE
Figure 1: Function Indicators & Keys
Function Keys
Function
Indicators Function Display
Function Keys