WPI MPS-4 User manual

WORLD
PRECISION
INSTRUMENTS
MPS-4
Multichannel Perfusion System
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
011720

MPS-4
World Precision Instruments i
Copyright © 2020 by World Precision Instruments. All rights reserved. No part of this publication may
be reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 1
Notes and Warnings................................................................................................................. 2
Parts List...................................................................................................................................... 3
Unpacking................................................................................................................................... 4
INSTRUMENT DESCRIPTION ........................................................................................................ 4
Hardware Installation............................................................................................................... 4
OPERATING INSTRUCTIONS......................................................................................................... 5
Software Installation................................................................................................................. 6
Installation............................................................................................................................. 6
Startup ................................................................................................................................... 6
Creating a New Perfusion Experiment ................................................................................ 6
Set the Experiment Time......................................................................................................... 7
Preset Each Channel’s Perfusion Time ................................................................................ 7
Saving Your Experimental Parameters................................................................................ 9
Mode Selection .................................................................................................................... 9
Computer Perfusion Control Modes............................................................................10
Run Perfusion.....................................................................................................................10
Hardware Testing Procedure...............................................................................................11
Testing Drug Delivery.............................................................................................................12
MAINTENANCE ..............................................................................................................................13
Cleaning.....................................................................................................................................13
TROUBLESHOOTING ...................................................................................................................13
APPENDIX A: DETERMINING FLOW RATE................................................................................14
Theoretical Calculation ..........................................................................................................14
APPENDIX B: DISABLE DRIVER SIGNATURE ENFORCEMENT (WIN10) .............................15
APPENDIX C: HOW TO INSTALL AN UNSIGNED DRIVER ON WINDOWS 8 ..................... 19
APPENDIX D: INSTALL THE MPS-4 SOFTWARE ON WINDOWS 10....................................22
WARRANTY .....................................................................................................................................31
Claims and Returns ................................................................................................................31
Repairs.......................................................................................................................................31

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ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The MPS-4 controller operates the eight channels.
INTRODUCTION
The MPS-4 is a programmable 8-channel perfusion system designed for single
channel and whole-cell patch preparations. It oers the best combination of
performance and value. The improvement of MPS-4 over the previous MPS-2 is
the use of a diaphragm type solenoid valve that completely isolates the perfusion
solution from the outside to avoid the inuence of metal ions in the moving iron of
the solenoid valve on the experimental results. Unlike other perfusion systems on the
market, which often compromise performance to t every possible application, the
MPS-4 is the only perfusion system designed and optimized specically for single-
channel and whole-cell patch perfusion applications.
The system can be controlled manually (i.e., via membrane switches on the front
panel) or through a PC. Two dierent manual control modes are oered. One controls
each channel independently and the other mode allows sets channel 8 as the master
channel that will keep the system owing when all other channels are switched o.
User-friendly timing software is included, and the programmed perfusion sequence
can be started by computer, a TTL trigger from an external source such as a patch
clamp amplier or manually by the user. The high level (ON= +3 ~ +5V) and low level
(OFF = 0 ~ +1V) control mode that permits independent control of each valve by the
external instruments or data acquisition system.

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The perfusion uid ows through specially designed, color-coded, polyurethane
ribbon style tubing. The color-coding allows you to easily trace each channel for
diagnostic or set up. The ribbon style of the tubing keeps the system neat and clean.
Unlike PVC based tubing, polyurethane tubing contains no plasticizer, which can cause
contamination.
The most unique feature of the MPS-4 is its perfusion µ-manifold. Using the latest
microuidic techniques, the injection molded µ-manifold provides the least ow
resistance and dead volume of any product on the market. The ow channel inner
diameter is approximately 1 mm, except for the last 5 mm before the junction point.
This design allows a fast ow rate without using a pressurized system. The maximum
ow rates are 1 and 16 µL/s for the 15 mm long 100 µm and 250 µm ID tips,
respectively. Small channels and a unique design at the merging point further reduce
the chance of cross contamination. Dead volume is less than 100 nL. The injection-
molded µ-manifold is also designed as an economical disposable item, eliminating
problems of cross-contamination from other experiments.
Notes and Warnings
NOTE: Before starting a formal experiment, perform several preliminary tests, such as
the drug interaction range test, to get familiar with this perfusion system.
NOTE: The 100 and 250 µm perfusion manifold tips are made of ne glass capillary,
which is subject to breakage and clogging. Handle the tip carefully during the
experiment.
CAUTION: Tubing must be removed gently so that the manifold is not
damaged.
CAUTION: Any organic solvent, including alcohol, may damage the perfusion
manifold.
CAUTION: All the drug solutions should be ltered before use to prevent
clogging of the perfusion head.
CAUTION: After the experiment, the tubing system (especially the
electromagnetic valve, manifold and perfusion head) should be thoroughly
washed with warm, fresh distilled water as soon as possible. Failure to do so
may cause damage to the system. See “Cleaning” on page 13.
CAUTION: If the MPS-4 system does not work properly, stop the experiment
immediately. Switch o the power. See “Troubleshooting” on page 13.

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Parts List
After unpacking, verify that there is no visible damage to the sensor.
Verify that all items are included:
(1) Stand base and Stainless Steel Post
(1) MPS-4 Controller
(1) Valve Console
(1) Syringe Holder
(1) Power Cord
(1) USB Cable
(2) 1A Fuse
(10) 10mL Syringes
(10) 3-way Stopcock
(10) Luer tting with barb for 1/16” ID tubing
(5’) Color Coded Polyurethane Tubing Ribbon
(1) µ-manifold Holding Rod
(1) µ-manifold with 100µm ID tip
(1) µ-manifold with 250µm ID tip
(1) Installation Software
(1) Instruction Manual
Valve Console
Syringe Holder
Stand, post
3-way Stopcock
(#3744-100) 10 mL Syringe
(#3744-100)
Luer Fitting for 1/16” ID
tubing (#13156-100)
µ-Manifold
100µm: #502110
250µm: #502125
Manifold Holder
Fig. 2—These components are included with the MPS-4 system.

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Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 31 of this manual. Please contact WPI Customer
Service if any parts are missing at 941.371.1003 or [email protected].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary,
use the original container, if possible. If the original container is not available, use a
suitable substitute that is rigid and of adequate size. Wrap the instrument in paper or
plastic surrounded with at least 100mm (four inches) of shock absorbing material. For
further details, please read the section entitled “Claims and Returns” on page 31 of
this manual.
INSTRUMENT DESCRIPTION
Hardware Installation
1. Set the MPS-4 perfusion stand on a stable platform.
2. Insert the stainless steel post into the base and tighten the screw.
3. Loosen the screw on the back of the valve console and x it onto the post. Set
the distance between the valve console center point and top of the post to the
desired height.
4. Fasten the syringe holder onto the post.
5. Connect the valve console to the MPS-4 instrument with the cable supplied.
6. Pull out of the plungers of the syringes and put the syringes into the syringe
holder.
7. Connect the 3-way stopcocks to the syringes.
8. Cut a 20cm long section of the color-coded polyurethane tubing. Split it to 8
individual tubes and put the luer tting to one end. Connect the luer tting to the
3-way stopcock.
9. Connect the other ends of the eight tubes to the inputs of the valve console.
10. Split one end of the long color-coded polyurethane tubing for about 15cm.
Connect the splited end to the valve output port on the valve console.
11. Carefully connect the other ends of the polyurethane tubing to the µ-manifold.
CAUTION: In this step, the manifold should not be connected to the holding
rod. The female luer port may be damaged by the force applied to the manifold
when attaching the 8 ends of the tubing.

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12. Connect the stainless holder rod to the µ-manifold. The end of the holder rod is
a male luer tting. It can be pressed into the center hole of the µ-manifold for a
secure hold. Fix the holder to a micromanipulator.
TIP: We recommend WPI’s KITE-L for this purpose. It has sucient precision for
the perfusion tip and an economical price. It has a left hand scale for placing on
the left side of the microscope, leaving the right side free for the patch pipette.
13. Connect the MPS-4 to a computer USB port.
14. Connect a cable with a BNC plug to the socket on the back panel of MPS-4
controller corresponding to the channel to be controlled. Connect the other end
of the cable to the equipment that generates the control signal.
OPERATING INSTRUCTIONS
Fig. 3—The front panel of the MPS-4 has the operational controls.
The left section of the MPS-4 front panel contains the Start, Reset and Mode buttons,
and three functional indicator LEDs.
Start button–The button runs the perfusion process. In the Online mode,
it acts as the button and runs the experiment controlled by the
perfusion software. In Oine mode, the Start button runs the preset
perfusion parameters saved to the RAM of the control box from the
computer.
Reset button–This button is the zero button for the micro-controller. It
stops any currently running experiment, turns all channels o and resets
the mode to Manual. Press the button and hold it for 1 second before
lifting it to ensure the system is completely reset.
Mode button–This button toggles the perfusion mode between Manual,
Online, Oine and Master Manual. Once a mode is selected, the
corresponding LED illuminates. In Master Manual mode, the Manual LED
blinks continuously.

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Fig. 4—The Mode button toggles between the four operational modes.
Software Installation
System Requirements: Windows 7, 8, 8.1 or 10 PC, 10 Mb free hard drive space; CD-
ROM or DVD-ROM driver.
Installation
NOTE: The installer must have administrative privileges on the computer.
For complete instructions, see:
• “Appendix B: Disable driver signature enforcement (Win10)” on page 15
• “Appendix C: How to install an unsigned driver on Windows 8” on page 19
• “Appendix D: Install the MPS-4 software on Windows 10” on page 22.
Startup
The perfusion software can be found in the Start menu of Windows under C:\Program
Files (x86). The software automatically connects to the MPS-4 on startup. If the MPS-4
is plugged into the computer after the program has been loaded, press the button
to establish a connection.
Creating a New Perfusion Experiment
The Experiment menu allows you to open a new or
existing perfusion experiment.
After clicking on “New Experiment”, two new
windows pop up to allow you to set the experimental
parameters.