Endress+Hauser Analytik Jena Biometra TAdvanced 96 User manual

Operating Manual
Biometra TAdvanced
High-performance thermal cycler for
DNA amplification by PCR

Biometra GmbH (Analytik Jena AG)
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6-0
Fax + 49 551 50 68 6-66
Email [e-mail]
Biometra GmbH
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6 -10, -14 or -16
Email [e-mail]
http://www.analytik-jena.com
Operating manual valid from software version ME 1.70 –RE 1.46
Microsoft and Windows are registered trademarks of Microsoft Corp.
The identification with ® or TM is omitted in this manual.
34-3800-000-23-e
02_20
Analytik Jena AG
© Copyright 2020 Analytik Jena AG
Manufacturer
Service
General information
Copyrights and
Trademarks
Documentation number
Edition
Implementation of the
Technical Documentation

Biometra TAdvanced Manual Contents
Version 02_20 3
Contents
1Basic information ....................................................................................................... 9
1.1 User manual notes............................................................................................................ 9
1.2 Intended use ................................................................................................................... 10
1.3 Warranty and liability..................................................................................................... 10
2Safety instructions.................................................................................................... 11
2.1 General notes.................................................................................................................. 11
2.2 Safety markings on the Biometra TAdvanced .............................................................. 11
2.3 Technical condition ........................................................................................................ 11
2.4 Requirements for the operating personnel................................................................... 12
2.5 Safety instructions: transport and installation ............................................................. 12
2.6 Safety instructions –operation...................................................................................... 12
2.6.1 General............................................................................................................................ 12
2.6.2 Safety instructions: protection against explosion and fire........................................... 13
2.6.3 Safety instructions: electrical equipment ...................................................................... 13
2.6.4 Handling of samples, auxiliary and operating materials ............................................. 14
2.7 Safety instructions –maintenance and repair.............................................................. 14
2.8 Behavior during emergencies ........................................................................................ 14
3Function and setup................................................................................................... 15
3.1 High speed thermoblock ................................................................................................ 16
3.2 High Performance Smart Lid.......................................................................................... 16
3.3 Display............................................................................................................................. 17
3.4 User-specific quick start of programs ............................................................................ 17
3.5Easy programming.......................................................................................................... 17
3.6 Linear Gradient Tool....................................................................................................... 18
3.7 GLP compliance .............................................................................................................. 18
3.8 Open system philosophy ................................................................................................ 18
3.9 Order numbers................................................................................................................ 19
4Installation and commissioning ............................................................................... 20
4.1 Scope of delivery............................................................................................................. 20
4.2 Unpacking and inspection.............................................................................................. 20
4.3 Setting the operating voltage ........................................................................................ 20
4.4 Commissioning ............................................................................................................... 21
5Control elements ...................................................................................................... 22
5.1 Biometra TAdvanced front view.................................................................................... 22
5.2 Biometra TAdvanced rear view...................................................................................... 23
5.3 Quick block exchange..................................................................................................... 23
5.4 High Performance Smart Lid (HPSL)............................................................................. 24
5.4.1 Closing the lid ................................................................................................................. 24
5.4.2 Opening the lid ............................................................................................................... 25
5.5 The Biometra TAdvanced software ............................................................................... 26
5.5.1 Keyboard ......................................................................................................................... 26
5.5.2 Keypad............................................................................................................................. 27
5.5.3 Cursor keys ...................................................................................................................... 27
5.5.4 Confirm or Cancel ........................................................................................................... 27
5.5.5 List of commands and icons used.................................................................................. 28
6Operation.................................................................................................................. 33
6.1 Power on self-test .......................................................................................................... 33
6.2 Login screen.................................................................................................................... 33
6.3 Login................................................................................................................................ 34
6.4 Home screen ................................................................................................................... 35
6.5 Block status..................................................................................................................... 36
6.6 Quick start function ........................................................................................................ 37
6.7 Incubation mode............................................................................................................. 40

Contents Biometra TAdvanced Manual
4 Version 02_20
7Creating, editing and saving programs.................................................................... 44
7.1 Programming modes......................................................................................................44
7.2 Creating a new program/using a program template....................................................45
7.3 Assigning program names.............................................................................................. 47
7.4 Setting the heated lid temperature ...............................................................................47
7.5 Preheating the heated lid............................................................................................... 48
7.6 Editing steps.................................................................................................................... 49
7.6.1 Editing all parameters of a step.....................................................................................49
7.6.2 Direct programming of program parameters for a step...............................................56
7.7 Inserting a step ...............................................................................................................60
7.8 Deleting a step ................................................................................................................60
7.9 Saving the program ........................................................................................................60
8Starting, coping and deleting programs .................................................................. 63
8.1 Starting a program..........................................................................................................63
8.2 Copying a program..........................................................................................................65
8.3 Copying all programs......................................................................................................66
8.4 Deleting a program.........................................................................................................67
8.5 Deleting all programs .....................................................................................................68
9Running, pausing, continuing and stopping programs ........................................... 69
9.1 Display during a run........................................................................................................69
9.2 Pausing a program..........................................................................................................73
9.3 Continuing the program.................................................................................................75
9.4 Skipping a step................................................................................................................75
9.5 Stopping the program ....................................................................................................75
10 Protocol Wizard........................................................................................................ 77
10.1 Select Polymerase...........................................................................................................77
10.2 Enter a new polymerase or edit a polymerase.............................................................. 78
10.3 Delete a polymerase .......................................................................................................80
10.4 Protocol Wizard parameter screen ................................................................................80
10.5 Primer Annealing Temperature Calculator ...................................................................82
11 Tools ......................................................................................................................... 85
11.1 Configuration ..................................................................................................................85
11.1.1 Setting the date and time...............................................................................................87
11.1.2 Automatic user logout ....................................................................................................88
11.1.3 Configuring the beeper...................................................................................................89
11.1.4 Network...........................................................................................................................89
11.1.5 Display brightness...........................................................................................................91
11.1.6 Screen calibration ........................................................................................................... 92
11.1.7 Factory settings...............................................................................................................93
11.2 User management .......................................................................................................... 93
11.2.1 Creating a user ................................................................................................................95
11.2.2 Editing user settings .......................................................................................................96
11.2.3 Delete user ......................................................................................................................99
11.3 Documentation ............................................................................................................... 99
11.3.1 Run log file ....................................................................................................................100
11.3.2 Power On Logfile ..........................................................................................................102
11.3.3 Extended self-test log file ............................................................................................103
11.3.4 Error log file ..................................................................................................................104
11.4 Extended self-test.........................................................................................................104
11.5 Service info file (SINF)..................................................................................................107
11.6 Backup...........................................................................................................................108
11.7 Cycler Information ........................................................................................................109
11.8 Contact...........................................................................................................................110
12 Adaptation of programs......................................................................................... 111
13 Quick reference guide ............................................................................................ 112

Biometra TAdvanced Manual Contents
Version 02_20 5
14 Fault removal ......................................................................................................... 116
14.1 Administrator password forgotten ..............................................................................116
14.2 Slow heating and cooling.............................................................................................116
14.3 Auto restart...................................................................................................................116
14.4 Auto restart without an apparent cause .....................................................................116
14.5 Adaptation of programs from other thermal cyclers..................................................117
14.6 Loosening the lid wheel in case of blockage...............................................................117
14.7 Recommendations for the use of 384 well sample blocks.........................................118
15 Maintenance and care............................................................................................ 119
15.1 Cleaning the housing....................................................................................................119
15.2 Disinfecting the device .................................................................................................120
15.3 Firmware update ..........................................................................................................120
16 Transport ................................................................................................................ 121
16.1 Device return information............................................................................................121
16.2 Packing the Biometra TAdvanced ...............................................................................122
17 Disposal .................................................................................................................. 123
18 Specifications.......................................................................................................... 124
18.1 Model specific data.......................................................................................................124
18.2 Specifications for all models ........................................................................................130
19 Declaration of conformity ...................................................................................... 131
20 Index....................................................................................................................... 132
Figures
Fig. 1 The Biometra TAdvanced Thermal Cycler........................................................ 15
Fig. 2 Voltage selector switch on the underside of the device .................................. 20
Fig. 3 Front view of the Biometra TAdvanced............................................................ 22
Fig. 4 Rear view of the Biometra TAdvanced ............................................................. 23
Fig. 5 Quick block exchange ........................................................................................ 24
Fig. 6 Placement of sample tubes in different block systems ................................... 25
Fig. 7 Keyboard............................................................................................................. 26
Fig. 8 Keypad ................................................................................................................ 27
Fig. 9 Start screen......................................................................................................... 33
Fig. 10 Login screen ................................................................................................... 34
Fig. 11 Select user screen........................................................................................... 34
Fig. 12 Enter password screen................................................................................... 35
Fig. 13 Home screen................................................................................................... 35
Fig. 14 Block status/quick start button..................................................................... 36
Fig. 15 Program quick start screen (Biometra TAdvanced Mono).......................... 38
Fig. 16 Program quick start screen (Biometra TAdvanced Twin) ........................... 39
Fig. 17 Multiblock selection query ............................................................................ 40
Fig. 18 Incubation mode screen (Biometra TAdvanced Mono) .............................. 41
Fig. 19 Incubation mode screen (Biometra TAdvanced Twin)................................ 42
Fig. 20 Multiblock selection query (1) ...................................................................... 43
Fig. 21 Multiblock selection query (2) ...................................................................... 43
Fig. 22 Spreadsheet programming............................................................................ 44
Fig. 23 Graphical programming................................................................................. 44
Fig. 24 Program overview screen .............................................................................. 46
Fig. 25 BLANK program template ............................................................................... 46
Fig. 26 Program template screen .............................................................................. 47
Fig. 27 Heated lid temperature setting screen......................................................... 48

Contents Biometra TAdvanced Manual
6 Version 02_20
Fig. 28 Program step editing screen......................................................................... 50
Fig. 29 PCR program with a loop .............................................................................. 51
Fig. 30 Temperature gradient on a 96 well sample block....................................... 52
Fig. 31 Linear Gradient Tool screen (Biometra TAdvanced Mono)........................ 53
Fig. 32 Standard gradient programming screen (Biometra TAdvanced Mono).... 54
Fig. 33 Temperature gradient on a 48 well sample block....................................... 54
Fig. 34 Linear Gradient Tool screen (Biometra TAdvanced Twin) ......................... 55
Fig. 35 Standard gradient programming screen (Biometra TAdvanced Twin) ..... 56
Fig. 36 Temperature gradient query......................................................................... 56
Fig. 37 Spreadsheet program view ........................................................................... 57
Fig. 38 Graphical program view ................................................................................ 58
Fig. 39 Individual program parameter editing......................................................... 59
Fig. 40 Gradient view (Biometra TAdvanced Twin) ................................................ 59
Fig. 41 Program overview screen.............................................................................. 61
Fig. 42 Quick selection screen ................................................................................... 62
Fig. 43 Program overview screen.............................................................................. 63
Fig. 44 Program overview screen with preview ....................................................... 64
Fig. 45 Twinblock selection query............................................................................. 64
Fig. 46 Program overview screen.............................................................................. 66
Fig. 47 Program overview screen.............................................................................. 67
Fig. 48 Confirmation prompt..................................................................................... 68
Fig. 49 Confirmation prompt..................................................................................... 68
Fig. 50 Graphical view ............................................................................................... 69
Fig. 51 Spreadsheet view........................................................................................... 70
Fig. 52 Display during the run in a graphical, spreadsheet and gradient view...... 70
Fig. 53 Graphical view ............................................................................................... 71
Fig. 54 Spreadsheet view........................................................................................... 71
Fig. 55 Display during the run in a graphical, spreadsheet and gradient view...... 72
Fig. 56 Spreadsheet view........................................................................................... 73
Fig. 57 Display screen during a program pause (graphical view)........................... 73
Fig. 58 Spreadsheet view........................................................................................... 74
Fig. 59 Display screen during a program pause (graphical view)........................... 74
Fig. 60 Confirmation prompt..................................................................................... 76
Fig. 61 Twinblock selection query............................................................................. 76
Fig. 62 Confirmation prompt..................................................................................... 76
Fig. 63 Select polymerase screen .............................................................................. 77
Fig. 64 Edit polymerase screen ................................................................................. 78
Fig. 65 Select polymerase screen .............................................................................. 79
Fig. 66 Security query ................................................................................................ 79
Fig. 67 Protocol wizard parameter screen................................................................ 81
Fig. 68 Protocol wizard view parameter screen....................................................... 81
Fig. 69 Security query ................................................................................................ 82
Fig. 70 Primer annealing temperature calculator screen........................................ 83
Fig. 71 Edit primer concentration screen ................................................................. 83
Fig. 72 Tools screen ................................................................................................... 85
Fig. 73 Settings screen............................................................................................... 86
Fig. 74 Date and time entry screen........................................................................... 87
Fig. 75 Automatic user logout screen ....................................................................... 88
Fig. 76 Beeper configuration screen......................................................................... 89
Fig. 77 Network settings screen, IP settings via static ............................................ 90
Fig. 78 Network settings screen, IP settings via DHCP............................................ 90
Fig. 79 Display brightness adjustment screen ......................................................... 92
Fig. 80 Calibration screen .......................................................................................... 92
Fig. 81 Confirmation prompt..................................................................................... 93

Biometra TAdvanced Manual Contents
Version 02_20 7
Fig. 82 User management screen.............................................................................. 94
Fig. 83 Configure user management screen............................................................. 94
Fig. 84 Create new user screen.................................................................................. 96
Fig. 85 User data screen............................................................................................. 97
Fig. 86 User properties screen ................................................................................... 98
Fig. 87 Confirmation prompt ..................................................................................... 99
Fig. 88 Documentation screen.................................................................................100
Fig. 89 Run log file overview screen........................................................................100
Fig. 90 Run log file screen........................................................................................101
Fig. 91 Program view screen ...................................................................................102
Fig. 92 Power on log file screen ..............................................................................103
Fig. 93 Extended self-test log file screen................................................................103
Fig. 94 Error log file screen......................................................................................104
Fig. 95 Sample block loaded with plasticware for the extended self-test............105
Fig. 96 Extended self-test screen ............................................................................106
Fig. 97 Extended self-test overview screen (Biometra TAdvanced Mono)..........106
Fig. 98 Extended self-test overview screen (Biometra TAdvanced Twin) ...........107
Fig. 99 Service info file creation screen ..................................................................107
Fig. 100 Backup file screen ........................................................................................108
Fig. 101 Cycler Info screen .........................................................................................109
Fig. 102 Service department contact details screen.................................................110

Contents Biometra TAdvanced Manual
8 Version 02_20

Biometra TAdvanced Basic information
Version 02_20 9
1Basic information
1.1 User manual notes
The Biometra TAdvanced is intended for operation by qualified specialist personnel
observing this operating manual.
For the Biometra TAdvanced, there are 12 different models available. In the text
below these devices are collectively called Biometra TAdvanced. Differences are
explained in the corresponding sections.
The operating manual contains information about the design and operation of the
Biometra TAdvanced and provides personnel familiar with PCR technology with the
necessary know-how for the safe handling of the equipment and its components. The
operating manual further includes notes on the maintenance and service of the device
and potential causes and remedies of any faults.
These operating instructions apply to all Biometra TAdvanced as of software version
ME 1.70 - VRE 1.46 (see "Cycler Information" p.109)
Furthermore, sometimes the terms protocol and program are used synonymously,
these are one or more programmed temperature steps with different or the same
holding times and cycles.
Instructions for actions which occur in chronological order are numbered and
combined in action units.
Safety instructions are indicated by pictographs and signal words. The type and source
of the danger are stated together with notes on preventing the danger.
The elements of the control and analysis program are indicated as follows:
Program terms are identified with SMALL CAPS (e.g., Menu FILE).
Buttons are shown by square brackets (e.g., [OK] button)
Menu items are separated by arrows (e.g., FILE OPEN).
The operating manual uses the following symbols and signal words to indicate hazards
or instructions. The safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation which might cause fatal or very serious
injuries (deformities).
CAUTION
Indicates a potentially hazardous situation which might cause light or minor injuries.
NOTICE
Provides indications of potential material and environmental damage.
Conventions
Symbols and signal words

Basic information Biometra TAdvanced
10 Version 02_20
Symbol for waste disposal in accordance with WEEE directive:
Do not dispose of in household waste.
CE marking
China RoHS marking
1.2 Intended use
The Biometra TAdvanced is an end-point thermal cycler. It is designed to amplify
nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a
PCR reaction.
The thermal cycler is developed for Research Use Only (RUO).
The Biometra TAdvanced combines modern design with user-friendly software. The
user interface consists of a touchscreen with a graphical or spreadsheet display that
shows the time, the status and the temperature program for each run. Using the
touchscreen keyboard, information and program parameters can be entered directly
on the screen. Due to the high ramp rates, the Biometra TAdvanced thermal cycler is
suitable for fast PCR applications, thereby helping to shorten program run times.
1.3 Warranty and liability
The warranty duration and liability comply with the legal requirements and the
provisions in the general terms and conditions of Analytik Jena AG.
Deviations from the intended use described in this manual result in limitations of
warranty and liability in the event of damage.
Warranty and liability claims are excluded for personal injury and property damage
due to one or several of the following causes:
use of the Biometra TAdvanced other than intended
improper commissioning, operation and servicing of the device
modifications of the Biometra TAdvanced without prior consultation with Biometra
GmbH
unauthorized intervention in the Biometra TAdvanced
operation of the Biometra TAdvanced with faulty safety equipment
use of other than original spare parts, wearing parts or consumables
improper repairs
improper transport and storage of the Biometra TAdvanced
faults due to the non-observance of this manual

Biometra TAdvanced Safety instructions
Version 02_20 11
2Safety instructions
2.1 General notes
For your own safety and to ensure error-free and safe operation of the Biometra
TAdvanced, please read this section carefully before commissioning.
Besides the safety instructions in this user manual and the local safety regulations that
apply to the operation of the device, the general applicable regulations regarding
accident prevention, occupational health and safety and environmental protection
have to be observed and complied with.
References to potential dangers do not replace the work protection regulations which
must be observed.
2.2 Safety markings on the Biometra TAdvanced
Damaged or missing safety symbols can cause incorrect actions leading to personal
injury or material damage. Do not remove safety symbols! Replace damaged safety
symbols immediately!
The following safety symbols are attached to the inside of the heated lid and the rear
of the device:
CAUTION! Hazard area!
Proceed with due care when handling samples and working with the Biometra
TAdvanced.
CAUTION! Risk of burns!
There is a risk of burning at the heated lid, the thermal block, the samples, and the
rear of the device.
The following sign is attached to the underside of the Biometra TAdvanced:
ATTENTION! Keep ventilation slits clear!
Keep the ventilation slits on the underside and at the rear of the device clear. Make
sure that there are no objects underneath the device (e.g., paper) that may be sucked
against the ventilation slot and thus interfere with ventilation.
2.3 Technical condition
The Biometra TAdvanced has been built and certified according to safety standard
EN 61010-1. Do not modify the device in any way. Any modification of the device will
lead to a loss of warranty and the EN 61010-1 certificate and poses a potential risk.

Safety instructions Biometra TAdvanced
12 Version 02_20
The following has to be observed:
The operator must only operate the device in a sound and operationally safe
condition. The technical condition must always comply with the legal requirements
and regulations.
Prior to every use the device must be checked for damage and sound condition.
Any changes in the device affecting its safety must be reported by the operating
personnel to the operator without delay.
2.4 Requirements for the operating personnel
The Biometra TAdvanced must only be operated by qualified specialist personnel
instructed in the use of the device. The instructions also include imparting the contents
of this manual.
In addition to the safety at work instructions in this operating manual the generally
applicable safety and accident prevention regulations of the respective country of
operation must be observed and adhered to. The operator must ascertain the latest
version of these regulations.
The operating manual must be accessible to the operating and service personnel at all
times.
2.5 Safety instructions: transport and installation
The following has to be observed:
Drain the sample block before transporting the Biometra TAdvanced. Make sure
there are no sample tubes in the block.
Only ship the Biometra TAdvanced in its original packaging with the transport lock
installed.
Please verify that the delivery is complete on receipt of the Biometra TAdvanced
and check for possible transport damage. In case of claims please contact Biometra
GmbH.
2.6 Safety instructions –operation
2.6.1 General
The operator of the Biometra TAdvanced must make sure before each commissioning
that the condition of the device including the safety equipment is sound.
The following has to be observed:
Free access to the power switch on the back of the enclosure has to be ensured
during operation.

Biometra TAdvanced Safety instructions
Version 02_20 13
The ventilation fittings at the rear of the device must be unobstructed and
operational. Covered ventilation grilles or slits etc. may cause the device to break
down or may cause damage to it.
The use of oil between the samples and the sample block is not necessary to
achieve an improved heat exchange. However, if you still want to use oil, you
should use mineral oil. Do not use silicone oil.
Be careful to avoid crushing or pinching injuries when closing the device.
The Biometra TAdvanced may cause burns. The following has to be observed:
The thermal block, the samples and the heated lid reach high temperatures. There
is a risk of burns during contact.
Ensure that the lid is securely closed before starting the program! Do not touch the
heated lid!
The rapid heating of the thermal block can cause liquids to boil explosively. Always
wear safety goggles during operation!
Do not touch hot sample tubes or plates and do not open them or boiling liquid
may escape!
Only use plates and tubes that are suitable for high temperatures (up to 100°C), fit
well into the thermal block (no shaking) and whose lids seal tightly!
2.6.2 Safety instructions: protection against explosion and fire
The Biometra TAdvanced must not be operated in an explosive environment.
The Biometra TAdvanced must not be operated with flammable, explosive or volatile
substances.
2.6.3 Safety instructions: electrical equipment
Work on the electrical components of the Biometra TAdvanced may only be performed
by a qualified electrical technician in accordance with the applicable electrical
engineering rules. Life-threatening electrical voltages may occur in the interior of the
Biometra TAdvanced!
The following has to be observed:
Any work on the interior of the device may only be carried out by the customer
service of Biometra GmbH and specially authorized technicians.
The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections, faulty or damaged cables, must be repaired
without delay.
Before opening the device it must be switched off at the power switch and the
mains plug must be disconnected from the mains outlet!
The Biometra TAdvanced must be switched off immediately using the power switch
(on the equipment back plate) and the mains plug disconnected from the power
supply if any faults occur in the electric components.

Safety instructions Biometra TAdvanced
14 Version 02_20
Do not operate the device near sources of strong electromagnetic radiation (for
example, an unshielded, deliberately operated high frequency source), as these
may affect proper operation of the device.
2.6.4 Handling of samples, auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well
as for their safe handling. This is particularly important for radioactive, pathogenic,
infectious, poisonous, corrosive or otherwise dangerous substances. For details contact
the safety officer responsible for your location. When handling dangerous substances
local safety codes and guidelines must be observed. The following general notes do
not replace the specific local regulations or the regulations in the EC safety data sheets
of the manufacturers for the auxiliary and operating materials.
The following has to be observed:
Protective goggles and rubber gloves have to be worn when handing reagents.
If only a few samples are treated, an (empty) tube of the same height must
additionally be placed at each corner position of the block. If the number of
samples in the block is too low, there is a risk of the tubes being damaged and
sample liquid leaking out.
For your own safety, please observe the potential infectious qualities of the
examined biological material.
2.7 Safety instructions –maintenance and repair
The Biometra TAdvanced is usually repaired by the service department of Biometra
GmbH or its authorized and trained specialist personnel. Unauthorized repairs can
damage the device. Therefore, the operator may generally only carry out the tasks
listed in section "Maintenance and care" p. 119.
The following has to be observed:
The exterior of the device may only be cleaned with a damp, not dripping, cloth
after the device has been switched off.
Do not use alcohol (e.g., methanol or ethanol), organic solvents or abrasives to
clean the device.
Any maintenance on the device may usually only be carried out in the switched-off
condition (unless stated otherwise).
2.8 Behavior during emergencies
In case of danger or accidents, immediately switch off the Biometra TAdvanced using
the main switch on the equipment back plate. Disconnect the mains plug from the
power supply!

Biometra TAdvanced Function and setup
Version 02_20 15
3Function and setup
The Biometra TAdvanced thermal cycler is available in the following models:
Model
Biometra TAdvanced 96
with aluminum block
Biometra TAdvanced 96 G
with aluminum block
with gradient function
Biometra TAdvanced 96 S
with silver block
Biometra TAdvanced 96 SG
with silver block
with gradient function
Biometra TAdvanced 60
with aluminum block
Biometra TAdvanced 60 G
with aluminum block
with gradient function
Biometra TAdvanced 384
with aluminum block
Biometra TAdvanced 384 G
with aluminum block
with gradient function
Biometra TAdvanced Twin 48
with aluminum twinblock
Biometra TAdvanced Twin 48 G
with aluminum twinblock
with gradient function
Biometra TAdvanced Twin 30
with aluminum twinblock
Biometra TAdvanced Twin combi
with aluminum twinblock
The device is controlled by a 7'' touchscreen display and an easy-to-use user interface.
PCR protocols and run log files can be saved to a connected USB stick. By using the
USB functions, PCR protocols can be exchanged easily between devices and run log
files can be stored for documentation reasons on a PC.
Fig. 1 The Biometra TAdvanced Thermal Cycler

Function and setup Biometra TAdvanced
16 Version 02_20
The Biometra TAdvanced thermal cycler features an automatic restart. If a power
failure occurs during the run, the device will continue the run as soon as power is
restored. In case of long-term power failure (longer than 30 minutes), the device will
keep the sample block at 4 °C (freeze step) and the user can decide to repeat the run
with the same samples or to discard them.
The block with gradient function can optionally be used for the optimization of new
primer pairs. The Linear Gradient Tool allows the programming of gradients with a
defined temperature difference between the rows or columns of the sample block.
This manual provides information on how to use all models of the Biometra
TAdvanced Thermal Cycler most effectively. Functions that are only available for
certain versions are indicated accordingly, for example, "This function is only available
for gradient-enabled thermal cyclers".
3.1 High speed thermoblock
The Biometra TAdvanced Thermal Cycler is available with different block formats and
with quick block exchange technology that allows easy exchange of block modules in a
few seconds. Most blocks are made of aluminum and offer high ramp rates for fast
protocol running times. Additionally, to achieve ultimate performance, a 96 well block
made of silver is available. Due to its excellent heat conductivity silver equilibrates
extremely quickly, thus providing maximum speed and temperature uniformity. To
protect the valuable silver blocks against corrosion, the block surfaces are gold plated.
A rubber seal on the heated lid forms an encapsulated space around the sample block
as soon as the lid is closed. The closed space serves to improve the temperature
uniformity of the sample block and avoids the formation of condensed water at the
final PCR cooling step. Furthermore all blocks are perfectly sealed to prevent
condensed water from penetrating into the Peltier elements underneath the sample
block and other parts of the electronics. The sealing protects the Peltier elements and
prolongs the lifetime of the device.
3.2 High Performance Smart Lid
The Biometra TAdvanced heated lid has been optimized to heat twice as fast as with
older models and thus helps to shorten protocol run times. Moreover the heated lid
fulfills two other important functions: It prevents the formation of condensation at the
reaction tube portion located above the block surface level and it ensures reliable
contact between the reaction tubes and the thermoblock by applying constant
pressure. Thanks to the integrated clutch mechanism, the pressure applied to the
reaction tubes is always the same, regardless of the plasticware height. The
combination of the heated lid shape and the reliable contact pressure ensures even
temperature distribution between samples, thus significantly improving temperature
uniformity.
With one press on the front button, the heated lid gently swings open and locks in its
end position. This mechanism ensures that the lid cannot fall down and the heated lid
is out of the radius of action while the user inserts PCR tubes or plates. This prevents
any risk of injury from crushing or hot surfaces.

Biometra TAdvanced Function and setup
Version 02_20 17
3.3 Display
The Biometra TAdvanced thermal cycler features a 7" state-of-the-art color
touchscreen user interface. The touchscreen is built-in at a flat angle to ensure
reflection-free viewing and ergonomic programming. For programming PCR protocols
the software incorporates Biometra's proven spreadsheet philosophy and alternatively
offers graphical programming. One touch of a button leads from the spreadsheet to
the alternative graphical programming mode. This makes the creation of new
programs or editing existing programs fast and easy.
3.4 User-specific quick start of programs
Many other thermal cyclers offer a so-called "List of latest programs used". In the list of
latest programs used a certain number of PCR programs is collected and offered for
quick start. The list is typically ordered only by date and the user has to search for the
desired program. In contrast the Biometra TAdvanced software creates a user-specific
list and only the latest programs started by the currently logged-in user are offered for
quick start. Even if a lab member does not use the device for a longer time, the
information on the latest programs started by this user will not get lost.
For maximum convenience the Biometra TAdvanced software additionally features a
program preview. Before the start of a program the protocol steps are summarized in a
clearly arranged table by the program preview tool. The preview therefore provides a
comprehensive overview of the protocol structure without the need to access
programming screens. The program preview is also available before the quick start of
programs.
3.5 Easy programming
Creating new PCR programs takes a lot of time if for each single step all parameters
have to be set manually. The Biometra TAdvanced offers two options for easy protocol
creation:
Pre-installed program templates
Protocol wizard
The Biometra TAdvanced software offers several pre-installed program templates for
different applications. The program templates provide a general protocol structure and
can be easily adapted for the current experiment.
The protocol wizard offers the comfort to create specific PCR programs by just a few
inputs. The protocol wizard is based on program templates for specific polymerases.
For up to eight different polymerases program templates can be saved in the Biometra
TAdvanced software and just the annealing temperature, cycle number and product
length has to be filled in to create a specific PCR program. Optionally two-step or
three-step PCR programs can be created by the software. Furthermore the protocol
wizard includes a primer annealing temperature calculator and the calculated
annealing temperature Tavalue can be used for protocol creation.

Function and setup Biometra TAdvanced
18 Version 02_20
3.6 Linear Gradient Tool
For optimizing new primer pairs in a single run the Biometra TAdvanced can optionally
be equipped with a gradient function. Finding the best primer annealing temperature
is crucial for the specificity and efficiency of PCR reactions. Often the optimization of
experiments is conducted to a limited extent only leading to unspecific byproducts or
reduced PCR sensitivity. By using the gradient function, new primer pairs with
unknown annealing temperatures can be tested quickly and optimized in a very short
time.
For maximum convenience the Biometra TAdvanced offers the Linear Gradient Tool.
For most other devices the temperature difference from row to row or from column to
column in a gradient step varies across the sample block. The Biometra TAdvanced
Linear Gradient Tool enables the programming of gradient steps with a defined
temperature increment between the columns or rows. The Linear Gradient Tool option
allows the comfortable creation of gradient steps and thus the best annealing
temperature can be easily transformed to a non-gradient protocol.
3.7 GLP compliance
The software can manage up to 90 user accounts. With each system start the Biometra
TAdvanced performs an initial self-test and additionally the software offers an
extended self-test that can be initiated by the user. The results of the extended self-
test are summarized in a protocol and stored by the device. In addition to the self-test
functions, the device creates and stores log files for each single run. For long-term
storage, the log files and extended self-test protocols can be exported as a proprietary
file format.
3.8 Open system philosophy
The Biometra TAdvanced is an open system regarding consumables and reagents.
Depending on the sample block, the system can be used with single tubes, strips or
plates. PCR plates can be standard profile or low profile and they can be full-skirted,
half/semi-skirted or non-skirted. The Biometra TAdvanced can handle all kinds of
plates and the plasticware be sealed with domed lids, flat lids, sealing foil or other
technologies. Regardless of the sealing method used, thanks to the HPSL technology
(see section "High Performance Smart Lid" p. 16) the same pressure is always applied
to the consumables for absolutely reproducible conditions.
The Biometra TAdvanced thermal cycler is not limited to reagents from a specific
vendor and can be used with any kind of polymerase. Regardless of whether
isothermal, hot start or non-hot start polymerases are used, the Biometra TAdvanced
will produce reliable results. By programming a pause step for the initial denaturation,
for example, the system can be used for manual hot start applications. After inserting
the samples into the heating block, the PCR protocol can be started just by pressing
"continue".

Biometra TAdvanced Function and setup
Version 02_20 19
3.9 Order numbers
Instruments incl. block and base unit
Model
Block type
Gradient range
Block exchange
Order numbers
Biometra TAdvanced 96
Aluminum
–
846-x-070-211
Biometra TAdvanced 96 G
Aluminum
30 °C
846-x-070-201
Biometra TAdvanced 96 S
Silver
–
846-x-070-251
Biometra TAdvanced 96 SG
Silver
40 °C
846-x-070-241
Biometra TAdvanced 60
Aluminum
–
846-x-070-210
Biometra TAdvanced 60 G
Aluminum
30 °C
846-x-070-200
Biometra TAdvanced 384
Aluminum
–
846-x-070-224
Biometra TAdvanced 384 G
Aluminum
24 °C
846-x-070-214
Biometra TAdvanced Twin 48
Aluminum
–
846-x-070-212
Biometra TAdvanced Twin 48 G
Aluminum
20 °C
846-x-070-202
Biometra TAdvanced Twin 30
Aluminum
–
846-x-070-213
Biometra TAdvanced Twin combi
Aluminum
–
846-x-070-215
G = gradient, S = silver block
X = 2 for 230 V EN/DE, 4 for 115 V EN, 5 for 100 V EN/DE
Base unit and block modules
Block model
Block type
Gradient range
Block exchange
Order numbers
Block module 96
Aluminum
–
846-070-231
Block module 96 G
Aluminum
30 °C
846-070-221
Block module 96 S
Silver
–
846-070-271
Block module 96 SG
Silver
40 °C
846-070-261
Block module 60
Aluminum
–
846-070-230
Block module 60 G
Aluminum
30 °C
846-070-220
Block module 384
Aluminum
–
846-070-234
Block module 384 G
Aluminum
24 °C
846-070-224
Twinblock module 48
Aluminum
–
846-070-232
Twinblock module 48 G
Aluminum
20 °C
846-070-222
Twinblock module 30
Aluminum
–
846-070-233
Twinblock module combi
Aluminum
–
846-070-235
Base unit
TAdvanced base unit
846-x-070-280
G = gradient, S = silver block

Installation and commissioning Biometra TAdvanced
20 Version 02_20
4Installation and commissioning
4.1 Scope of delivery
The scope of delivery includes:
Thermal cycler
Mains cable
Operating instructions
Quick reference guide
Please keep the original packaging in case the device needs to be returned. The
shipping box for the Biometra TAdvanced thermal cycler contains a specially
developed foam system for the transport of high quality electronic devices.
4.2 Unpacking and inspection
Unpack and carefully check the device. Immediately report any damage to Biometra
GmbH. Do not attempt to operate the device if there is any visible damage.
Keep the original packaging for possible return. The Biometra TAdvanced must always
be transported in its original packaging. If the packaging is no longer available, please
contact Biometra GmbH.
4.3 Setting the operating voltage
WARNING
Danger of electric shock! Before commissioning the device, make sure that the voltage
selector setting on the underside of the device corresponds to the mains voltage in
your laboratory.
The Biometra TAdvanced can be operated at 100, 115 or 230 volts.
Set the operating voltage by turning the dial on the underside of the Biometra
TAdvanced to the desired voltage. You can do so with a coin.
Fig. 2 Voltage selector switch on the underside of the device