Integra D-ONE User manual

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Quick Start Guide D-ONE Single Channel Pipetting Module
This quick start guide is intended to provide a quick overview of your D-ONE single
channel pipetting module’s key features. For detailed information, please refer to the
latest operating instructions of D-ONE and ASSIST PLUS that can be found at
www.integra-biosciences.com in different languages.
Intended Use
This is a general-purpose laboratory instrument for use in research only. D-ONE is used on the
ASSIST PLUS pipetting robot for aspirating and dispensing aqueous liquids in the volume range of
0.5 –1250 μl using GripTip™ pipette tips only, see www.integra-biosciences.com. D-ONE provides
pressure-based liquid level detection and is compatible with two different GripTip sizes.
Safety information
Regardless of the listed safety notes, all locally applicable regulations must be observed.
1) This product may only be operated in a secure, protected network with trustworthy clients.
2) The pipette may only be used by properly trained personnel in a manner specified by INTEGRA
Biosciences.
3) Use only an INTEGRA battery and charging device.
4) Do not expose the battery to heat over 60 °C or mechanical stress.
5) Replace battery if the charging intervals are unusually short or if the charging takes much
longer than 4 hours.
6) Do not use or charge the instrument near flammable material or in an atmosphere with danger
of explosion.
7) Servicing work andrepairs may only be performed by INTEGRA Biosciences or an authorized
after-sales service member.
Battery Charge
Charge the Li-ion battery completely before first use
with an INTEGRA stand, cable or on ASSIST PLUS.
The supply voltage must meet the requirements of the mains
adapter: 100–240 VAC, 50/60 Hz.
Low Battery
Recharging Battery
Fully Charged Battery
~ 2.5 h
Blinking red
Blinking bars
Three solid bars
Getting Started
1. Battery charge indicator on display
2. Back button, to navigate backward
3. Touch wheel, spin to scroll the cursor
4. OK button, to make a selection
5. Arrow buttons, for selections
6. Purge button, to empty tips
7. RUN button, to start operations
8. Tip ejector
Turn on:
Press and release RUN.
Turn off:
Press and hold the Back button for 3 seconds.

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Language Selection
From the Main Menu, use the Touch Wheel to scroll to the Toolbox and press OK. Use the
“Language” option to select the desired language.
Pipette’s Toolbox
The Toolbox provides a variety of options, as setting personal preferences like the Touch wheel
spin sensitivity, computer connectivity and device information.
Creating a Pipetting Program
Use the VIALAB software to create multi-step programs on a PC and transfer them to the D-ONE,
see VIALAB operating instructions.
Starting a Pipetting Program
Use the communication module or the communication cable to connect D-ONE with ASSIST PLUS.
The following GripTips for benchtop pipetting systems can be attached:
D-ONE 0.5–300 μl (# 4531): 12.5 µl (pink) and 300 µl (green).
D-ONE 5–1250 μl (# 4532): 125 µl (yellow) and 1250 µl (blue).
Please visit the GripTip Selector Guide to find the correct GripTips.
From the Main Menu, select ASSIST PLUS. Press OK to enter the list of
VIALAB programs.
Select a program and press RUN.
Maintenance
Clean the outer surface with a lint-free cloth lightly soaked with mild soap solution in distilled water
or with a 70 % dilution of Isopropyl or Ethanol. Never use Acetone or other solvents.
D-ONE cannot be disassembled or autoclaved. Decontaminate D-ONE in accordance with good
laboratory practice. The device may be decontaminated with H2O2gas(maximalconcentration 35 %)
for 60 minutes.
If you intend to ship D-ONE to be periodically calibrated, the original packaging can be stored and
reused for this purpose.
Equipment Disposal
Electronic pipettes must not be disposed of with unsorted municipal waste.
Dispose of electronic pipettes in accordance with the regulations in your area governing
disposal of devices.
In certain regions and countries, e.g. in all EU member states, the distributor is obliged to
take back this product free of charge at the end of life. Please contact your local
distributor for more details.

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Manufacturer
INTEGRA Biosciences AG
Tardisstrasse 201
CH-7205 Zizers, Switzerland
T [phone]
[e-mail]
www.integra-biosciences.com
INTEGRA Biosciences Corp.
22 Friars Drive
Hudson, NH 03051, USA
T [phone]
INTEGRA Biosciences SAS
8 avenue du Fief
95310 Saint Ouen l'Aumône, FR
T [phone]
INTEGRA Biosciences Ltd
2 Rivermead Business Park
Thatcham, Berks, RG19 4EP, UK
T [phone]
INTEGRA Biosciences KK
Higashikanda 1-5-6, Chiyoda-ku
Tokyo, 101-0031, JP
T [phone]
INTEGRA Biosciences
Deutschland GmbH
An der Amtmannsmühle 1
35444 Biebertal, DE
T [phone]
INTEGRA Biosciences
(Shanghai) Co., Ltd.
Room 1110, No. 515 Huanke Road
Shanghai 201315, CN
T [phone]
Declaration of conformity
INTEGRA Biosciences AG –7205 Zizers, Switzerland
declares on its own responsibility that the devices
Description
Models
VIAFLO Pipettes
4011, 4012, 4013, 4014, 4015, 4016, 4621, 4622, 4623, 4624,
4626, 4631, 4632, 4633, 4634, 4636, 4641, 4642, 4646
VOYAGER Pipettes
4721, 4722, 4723, 4724, 4726, 4731, 4732, 4736, 4743, 4744,
4763, 4764
D-ONE Module
4531, 4532
Accessories
3214, 3215, 3216, 3217, 3218, 4200, 4205, 4210, 4211, 4215, 4221
comply with:
EU Directives and Regulations
2014/35/EU, 2014/30/EU, 2012/19/EC, 2011/65/EC, 2006/66/EC, 1907/2006, 2019/1782,
1103/2010
GBR Regulations
S.I. 2016/1101, S.I. 2016/1091, S.I: 2008/2164, S.I. 2013/3113, S.I. 2012/3032
Zizers, May 2, 2022
Urs Hartmann
CEO
Daniel Bächi
Head of Corporate Quality
For detailed CE declaration with regulations of other countries, please refer to the D-ONE
operating instructions.