Integra ASSIST PLUS User manual

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Quick Start Guide ASSIST PLUS Pipetting Robot
This quick start guide is intended to provide a quick overview of your ASSIST PLUS’s
key features and to offer basic instructions for getting started. For detailed
information, please refer to the latest operating instructions (OI)
that can be found at
www.integra-biosciences.com in different languages.
Intended use
This is a general-purpose laboratory instrument. Any use of this instrument in a medical or IVD
setting is the sole responsibility of the user. ASSIST PLUS is used to perform pipetting operations
automatically with a VIAFLO multichannel pipette, a VOYAGER pipette or a D-ONE single channel
pipetting module attached.
Safety information
Regardless of the listed safety notes, all locally applicable regulations must be observed.
1) This product may only be operated in a secure, protected network with trustworthy clients.
2) The pipette may only be used by properly trained personnel in a manner specified by INTEGRA
Biosciences.
3) Observe the hazard warnings on the device. Keep the tower moving area free of any labware
and keep hands away.
4) Do not use or charge the instrument near flammable material or in an atmosphere with danger
of explosion. Also, do not pipette highly flammable liquids such as acetone or ether.
5) Servicing work and repairs may only be performed by INTEGRA Biosciences or an authorized
after-sales service member.
Getting started
Set up the instrument on a perfectly horizontal surface according to the IQ/OQ document
(PN 128953). Supply voltage: 100 Hz3 6– 47 240 VAC, – .
Insert the adapter cable in the socket on the left side of the ASSIST PLUS and connect the adapter
via a 3-core cable to the electricity mains.
a. Gripper, with lever to release the pipette
b. Tower, with pipette arm
c. Interfaces and main switch
d. Tip rack deck, for GRIPTIPS
e. Touch panel
f. Deck, with positions for labware
g. Instrument base
h. Waste bin, for tip ejection
i. Tip sensor, for tip monitoring
j. Reflector, for tip sensor
Switch on/off:
Press the main switch.
k. Arrow keys to move the pipette back and
fore (Y-axis), right and left (X-axis) and up
and down (Z-axis)
l. Start/pause key
m. Communication LED and error LED
n. On/standby key
i
g
c
d
e
f
a
j
h
k
l
m
n
b

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Switch on the device. Press the blinking start/pause key (l) to home the ASSIST PLUS.
Remove hands from ASSIST PLUS during homing and operation.
Adapting the pipette holder
Adapt the pipette holder to the used INTEGRA multichannel pipette or D-ONE:
•VIAFLO 8- or 16-channel: fold both silver levers (1, 2) down.
•VIAFLO 12-channel: pull the lower silver lever (2) down.
•VOYAGER or D-ONE: fold both silver levers (1, 2) up.
Attaching an electronic pipette
For communication with ASSIST PLUS, the communication module must be installed to the pipette.
Rotate the lower housing of the pipette by 90 degrees as displayed in the picture below.
•To install a pipette, the tip ejector opening must face the pipette
holder. Hold the pipette at an angle and insert it into the pipette
holder. Then lift up until the black gripper snaps in place.
•To release the pipette, lift the black gripper by pushing down the
lever.
Enabling ASSIST PLUS program and pairing
To display the ASSIST PLUS program in the main menu of the pipette, navigate to the Toolbox.
Select Preferences - Main Menu and select ASSIST PLUS (green ).
The first time a specific pipette is connected to ASSIST PLUS, both
instruments need to be paired.
•Scroll to the Toolbox and press OK.
•From the ASSIST PLUS menu select ComModule Pairing.
•Switch OFF and ON the ASSIST PLUS device and wait until the
message “Pairing successful” is displayed.
Connection between ASSIST PLUS and pipette
When selecting ASSIST PLUS in the Main Menu, the pipette establishes a connection with the
ASSIST PLUS. The communication symbol is displayed next to the battery indicator and on the
touch panel of the ASSIST PLUS the communication LED (m) lights up in blue.
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Running a program
Place all required labware (reservoir, plates, etc.) on the deck. D-ONE requires its own tip deck.
From the ASSIST PLUS menu select the program category and the program you would like to run.
•Insert the tip box in the correct orientation and select the first
row/column with tips. Press the RUN button of the pipette.
•Press the blinking start/pause key (l) on the ASSIST PLUS device. It
switches to solid white, the ASSIST PLUS homes and the program is
performed automatically.
A program can be paused by pressing the start/pause key (l). Either press the start/pause key again
to continue the program or press Abort on the pipette to abort the program.
Setting up pipetting programs
You can easily create multi-step programs with the VIALAB software on a PC or select a predefined
program on the pipette and adapt the parameters to your needs.
Program category
Description
VIALAB Programs
Contains all programs created with VIALAB software (the only option for D-
ONE).
Serial Dilution
Aspirate a transfer volume followed by a mix.
Repeat Dispense
Dispense multiple aliquots of the same volume.
Variable Dispense
Dispense multiple aliquots of different volumes.
Multi Aspirate
Aspirate multiple aliquots of different volumes.
Plate Copy
Transfer samples between plates of the same well number.
Reformat
Reformat 12, 24, 48, 96 and 384 well plates.
Custom Programs
Create multi-stepped user-defined pipetting programs.
ASSIST PLUS’ Toolbox
The Toolbox provides a variety of options, like pairing ASSIST PLUS with a pipette, device
information, tip monitoring, tip type, park position and more.
Maintenance
Always switch off the device and disconnected form the electricity supply when carrying
out maintenance work.
•Clean the ASSIST PLUS housing with a lint-free cloth lightly soaked with mild soap solution in
distilled water or with a 70 % solution of isopropanol or ethanol.
•To decontaminate the ASSIST PLUS, take out the waste bin (h) to the front, remove the waste
bin frame, the magnetically attached sensor reflector (j) and the decks. Wipe the clean
instrument surface with a lint-free cloth lightly soaked with a suitable disinfectant and wipe dry
directly.
•Decontaminate the sensor reflector by spraying and wiping it with 70% Ethanol and after a short
treatment time (1-2 minutes) wiping it dry.
•The aluminum decks and the waste bin with frame can be autoclaved at 121 °C for 20 min.
•The device may be decontaminated with H2O2gas (maximal concentration 35 %) for 60
minutes.

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Equipment Disposal
ASSIST PLUS must not be disposed of with unsorted municipal waste.
Dispose of ASSIST PLUS in accordance with the regulations in your area governing
disposal of devices.
In certain regions and countries, e.g. in EU member states, the distributor is obliged to
take back this product free of charge at the end of life. Please contact your local
distributor for more details.
Manufacturer and customer service
INTEGRA Biosciences AG
Tardisstrasse 201
CH-7205 Zizers, Switzerland
INTEGRA Biosciences Corp.
22 Friars Drive
Hudson, NH 03051, USA
Find your local representative:
www.integra-biosciences.com
[e-mail]
Direct sales country
Telephone
Mail
China
[phone]
[e-mail]
Denmark, Sweden
[phone]
[e-mail]
France
[phone]
[e-mail]
Germany, Austria
[phone]
[e-mail]
Japan
[phone]
[e-mail]
Switzerland
[phone]
[e-mail]
United Kingdom
[phone]
[e-mail]
United States,Canada
[phone]
[e-mail]
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the device
Description
Model
ASSIST PLUS
4505
Mains adapter
128909
complies with:
EU Directives and Regulations
2014/35/EU, 2014/30/EU, 2014/53/EU, 2012/19/EC, 2011/65/EC, 1907/2006, 2019/1782
GBR Regulations
S.I. 2016/1101, S.I. 2016/1091, S.I. 2017/1206, S.I. 2013/3113, S.I. 2012/3032
For detailed, signed CE declaration with regulations of other countries, please refer to the
operating instructions supplied with the device.