Integra MINI 96 User manual

MINI 96 Operating instructions
129950_V00

www.integra-biosciences.com
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
comply with:
Description Models
MINI 96 4801, 4802, 4803, 4804
EU Standards Scope
EN 9001:2015 Quality Management
EN 61010-1:2010 Safety general laboratory equipment
EN 61326-1:2013 Electromagnetic compatibility laboratory equipment
EN 61010-2-081:2015 Safety automatic laboratory equipment
EU Directives Scope Date effective
2014/35/EU Low voltage directive (LVD) 20.04.2016
2014/30/EU Electromagnetic compatibility (EMC) 20.04.2016
2012/19/EC Waste electrical and electronic equipment
(WEEE)
14.02.2014
2011/65/EC Restriction of hazardous substances (RoHS) 03.01.2013
EU Regulations Scope Date effective
1907/2006
Registration, evaluation, authorisation and
restriction of chemicals (REACH)
01.06.2007
GBR Standards Scope
BS 61010-1:2010 Safety general laboratory equipment
BS 61010-2-081:2015 Safety automatic laboratory equipment
BS 63000:2018 Restriction of hazardous substances (RoHS)
GBR Regulations Scope Date effective
S.I. 2016/1101
Electrical equipment safety 08.12.2016
S.I. 2016/1091 Electromagnetic compatibility (EMC) 08.12.2016
S.I. 2013/3113 Waste electrical and electronic equipment
(WEEE)
01.01.2019
S.I. 2012/3032 Restriction of hazardous substances (RoHS) 02.01.2013

INTEGRA Biosciences AG
MINI 96– Declaration of conformity
Zizers, February 19, 2021
USA Standards Scope
UL 61010-1:2012 Safety general laboratory equipment
UL 61010-2-081:2015 Safety automatic laboratory equipment
USA Regulations Scope
47 CFR Part 15 (FCC) Electromagnetic compatibility (EMC)
17 CFR Parts 240 & 249b Dodd frank “Conflict minerals”
27 CCR Parts 25102-
27001
Proposition 65: The safe drinking water and
toxic enforcement act
CAN Standards Scope
CSA-C22.2 No. 61010-1 Safety general laboratory equipment
CSA C22.2 No. 61010-2-
081
Safety automatic laboratory equipment
CHN Standards Scope
SJ/T 11364-2014 Restriction of hazardous substances (RoHS)
CHN Regulations Scope Date effective
Order 32/2016 Restriction of hazardous substances (RoHS) 01.07.2016
JPN Regulations Scope Date effective
PSE (Denan) Law
Electrical appliance and material safety law 01.01.2014
Urs Hartmann
CEO
Thomas Neher
Quality Manager

MINI 96 – Operating instructions V00
4 INTEGRA Biosciences AG
Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................7
1.2 Intended use.....................................................................................7
1.3 Safety notes .....................................................................................8
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................9
2.2 Overview of MINI 96.........................................................................9
2.2.1 MINI 96 device ......................................................................9
2.2.2 MINI 96 control unit.............................................................10
2.2.3 Display ................................................................................10
2.2.4 Touch wheel........................................................................11
2.2.5 Left and right arrow buttons ................................................11
2.2.6 PURGE button ....................................................................11
2.2.7 RUN button .........................................................................11
Chapter 3 Installation
3.1 Operating environment...................................................................12
3.2 Setting up and moving of the device ..............................................12
3.3 Installation of stages.......................................................................13
3.3.1 Base stage ..........................................................................13
3.3.2 Second stage ......................................................................13
3.3.3 Two position stage ..............................................................13
3.4 Settings - adapt your MINI 96.........................................................14
3.4.1 Position settings..................................................................14
3.4.2 Preferences.........................................................................15
3.4.3 Calibration & Services.........................................................17
3.4.4 Communications .................................................................18
3.4.5 Device Information ..............................................................18
3.4.6 Park Head ...........................................................................18
3.4.7 Language ............................................................................ 18
3.4.8 Write protect........................................................................19

MINI 96 – Operating instructions V00
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Chapter 4 Operation
4.1 Turn on/off the device.....................................................................20
4.2 Attaching and removing GripTips ...................................................20
4.2.1 Loading tips from a rack with 96 tips...................................20
4.2.2 Loading tips from a rack with 384 tips.................................20
4.2.3 Partially tips loading ............................................................21
4.2.4 Ejecting used GripTips........................................................22
4.3 Start pipetting .................................................................................22
4.3.1 Pipetting ..............................................................................22
4.3.2 Setting pipetting height .......................................................22
4.3.3 Blowout/blowin modes ........................................................23
4.3.4 Recommendations for pipetting ..........................................24
4.3.5 Pipetting between 96-384 well plates .................................25
4.3.6 Pipetting with the Two position stage..................................25
4.4 Pipetting options and settings ........................................................26
4.4.1 Edit option ........................................................................... 26
4.4.2 Volume selection.................................................................26
4.4.3 Speed selection ..................................................................27
4.4.4 Custom................................................................................28
4.4.5 Pace....................................................................................28
4.4.6 Count, Mix Cycle and Rows................................................28
4.5 Troubleshooting/FAQ .....................................................................29
Chapter 5 Programs
5.1 Overview programs ........................................................................30
5.2 Detailed description of programs....................................................31
5.2.1 Pipet ...................................................................................31
5.2.2 Repeat dispense ................................................................32
5.2.3 Pipet/mix ............................................................................34
5.2.4 Sample dilute .....................................................................35
5.2.5 Manual pipet ......................................................................36
5.2.6 Reverse pipet .....................................................................37
5.2.7 Variable dispense ..............................................................38
5.2.8 Multi aspirate ......................................................................39
5.2.9 Sample dilute/mix ...............................................................40
5.2.10 Serial dilution program ......................................................41
5.3 Custom step-based program..........................................................42
5.3.1 Custom program .................................................................42
5.3.2 Modify existing programs ....................................................44

MINI 96 – Operating instructions V00
6 INTEGRA Biosciences AG
Chapter 6 Maintenance
6.1 Cleaning .........................................................................................45
6.2 Decontamination ............................................................................45
6.2.1 Two position stage ..............................................................45
6.3 Leak test.........................................................................................46
6.4 Servicing.........................................................................................47
6.4.1 Changing O-rings of tip fittings............................................47
6.5 Calibration ......................................................................................48
6.6 Equipment disposal ........................................................................48
Chapter 7 Technical Data
7.1 Environmental conditions ...............................................................49
7.2 Specification of the device..............................................................49
7.3 Intellectual Property........................................................................50
7.4 Pipetting specifications...................................................................50
7.5 Pipetting Speeds ............................................................................51
Chapter 8 Accessories and consumables
8.1 Accessories ....................................................................................52
8.2 Consumables .................................................................................53
Imprint ...................................................................................................57

MINI 96 – Operating instructions V00 Introduction
www.integra-biosciences.com 7
1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of MINI 96. This chapter informs about the symbols used in these
operating instructions, the intended use of MINI 96 and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
WARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
CAUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
NOTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
The device is marked with the following symbols:
BIOHAZARD
The device can be potentially biohazardous due to the use of biozazardous
substances by the operator.
CRUSHING OF HANDS
The hands may be squeezed, pulled in or otherwise injured by moving parts
of the device.
UNPLUG
The power cable must be unplugged before the housing of the device is
opened by a service technician.
1.2 Intended use
MINI 96 is an electronic 96 channel hand held pipette designed for aspirating and
dispensing aqueous solutions in the volume range of 0.5 µl to 1250 µl using GripTip
pipette tips. MINI 96 is used like a hand held pipette. The movement and positioning of the
pipette is supported by a servo assisted steering mechanism which allows fast, precise
and stress free multichannel pipetting.
Any use of this device in a medical or IVD setting is the responsibility of the user.
If MINI 96 is used in a manner not specified by INTEGRA Biosciences, the protection
provided by the MINI 96 may be impaired.

MINI 96 – Operating instructions V00 Introduction
8 INTEGRA Biosciences AG
1.3 Safety notes
MINI 96 complies to the recognized safety regulations and is safe to operate. MINI 96 can
only be operated when in perfect condition and while observing these operating
instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the MINI 96 must have read and understood
these operating instructions, and particularly, the safety notes, or must have been
instructed by supervisors so that safe operation of the device is guaranteed.
WARNING
Do not use the MINI 96 near flammable material or in explosive areas. Also,
do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
Do not immerse the pipette in liquid. The fluid can damage internal parts.
Avoid pipetting of liquids whose vapors could attack the materials PA
(polyamide), POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrile-
rubber), CR (chloroprene), silicone. Corrosive vapors could also damage
parts inside the device.
Do not open or modify MINI 96 in any way. The cover must not be removed.
Repairs may only be performed by INTEGRA Biosciences AG or by an
authorized after-sales service member.
Parts may be replaced with original INTEGRA Biosciences parts only.
NOTE
Prolonged exposure of the MINI 96 to UV-light can cause discoloration and/
or yellowing of the control unit. However, this will not affect the performance
of the device in any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

MINI 96 – Operating instructions V00 Description of the device
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2 Description of the device
2.1 Scope of delivery
• MINI 96 device
• Base stage
• Second stage
• Power cable
• Mains adapter
• Replacement O-rings and removal tool
(for 300 µl and 1250 µl instruments)
• Replacement Magnetic covers
• Quick start guide
CAUTION
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is damaged,
instead contact your local INTEGRA representative.
2.2 Overview of MINI 96
2.2.1 MINI 96 device
1Carrying grip
2Base stage on Deck
3Magnetic covers, for Second stage
4LED bars
5Deck light
6Control unit, see 2.2.2
7Carrying handle
8Pipetting unit, to move up and down
9AUX port
10 USB-C port
11 Main switch (ON ǀOFF)
12 DC Input for mains adapter
12
7
8
11
10
9
1
2
3
4
5
6

MINI 96 – Operating instructions V00 Description of the device
10 INTEGRA Biosciences AG
2.2.2 MINI 96 control unit
2.2.3 Display
The Display shows all pipetting programs.
13 Display
14 Back button, to navigate backward
15 Touch wheel, spin to scroll and move the cursor
16 OK button, to make a selection
17 Left and right arrow buttons, for selections
18 PURGE button, to empty tips
19 RUN button, to start operations
20 Tip ejector
Options are
listed in the
lower half
Run steps are
listed in the
upper half:
current program
instruction
active step
(orange)
OK to edit
Select and OK
to set pipetting
height
13
15
14
17
18 16
19
20
OK

MINI 96 – Operating instructions V00 Description of the device
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2.2.4 Touch wheel
The Touch wheel is fully operational with only one hand. Rotational finger movements
translate into up and down cursor movement on the display. The Touch wheel is fully
functional with the use of latex gloves.
2.2.5 Left and right arrow buttons
2.2.6 PURGE button
During pipetting, you can interrupt the current pipetting protocol and purge all remaining
liquid currently in the GripTips. To do so, press PURGE (18).
2.2.7 RUN button
Press and release RUN (19) to initiate aspiration, dispense, mix, purge, and special
pipetting operations.
During dispense, you can press and hold RUN to perform a two-step blowout, see “4.3.3
Blowout/blowin modes” on page 23.
Move finger on the Touch wheel to choose (and
highlight) an option on the display. Press OK (16) to
make the selection.
When a setting dial is displayed, spin the Touch wheel
to change the value and press OK.
At times, you will see and on a display screen. These buttons are
used to select options.
Press to select the option indicated with the left arrow (Help, in the
example beside). Press to select the option indicated with the right
arrow (Tutorials, in the example).
The pipette will display a prompt.
To proceed, press and release RUN. Upon completion of the dispense,
the first step in the current program will be displayed.

MINI 96 – Operating instructions V00 Installation
12 INTEGRA Biosciences AG
3 Installation
3.1 Operating environment
MINI 96 has been designed for use in a laboratory. It shall be operated in a dry and dust-
free location with a temperature of 5–40 °C and a maximal (non-condensing) relative
humidity of 80 %.
3.2 Setting up and moving of the device
The MINI 96 must be set up on a cleaned, dry and horizontal surface.
WARNING
The device must not be carried on the control unit because it will be
damaged.
Leave at least 5 cm of free space on the back for air circulation and to allow easy
connection of the mains cable.
Hold the Carrying grip (1) and handle (7) to lift the
MINI 96.

MINI 96 – Operating instructions V00 Installation
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3.3 Installation of stages
3.3.1 Base stage
Put the Base stage (2) on the two Deck pins. It can be placed on either side. The graphic
“96” or “384” must match the tip rack or well plate pattern.
3.3.2 Second stage
The Second stage can be used as a second position. Source liquids can be placed on
the Base stage, and the target, i.e. the well plate, can be slid on the Second stage for
filling.
3.3.3 Two position stage
The optional Two position stage (see "Accessories" on page 52) is required for partial tip
loading, filling partial plates or performing sample transfers and dilutions within a plate.
To install the Second stage, remove the two Magnetic
covers (3) by pressing on one side or using a GripTip.
Insert the Second stage with the desired side (96 or 384
format) facing upwards.
If it is noticed that the Second stage is not level, use a
1.27 hexagon screwdriver and tighten or loosen the two
screws (a) on the stage the same amount, to ensure that
both sides are the same height.
When the 96 side needs to be adjusted, screw on the 384
side and vice versa.
Tightening clockwise will move the stage very slightly
down.
Switch off the device.
Put the Two position stage on the two Deck pins.
Hold the device by the Carrying handle (7) and tilt it
slightly backwards. Fix the stage from the underside of
the deck with the large screw (b).
a
b

MINI 96 – Operating instructions V00 Installation
14 INTEGRA Biosciences AG
3.4 Settings - adapt your MINI 96
The Settings provides options to adapt the device to appropriate applications, setting
personal preferences, calibration, computer connectivity and storing owner information.
The Help information describes the programs and some settings, press to select the
Help option.
3.4.1 Position settings
Press OK to access. The desired setting is saved directly.
Settings mode Description
Position Settings Enables the ability to override the active pipette height.
Preferences Customizes the system parameters.
Calibration / Service Sets calibration and service history options.
Communications Enables communication between your MINI 96 and a PC.
Device Information View your pipette’s serial number, software version and set a
personal ID.
Park Head Fixes the pipetting unit for safe shipping.
Language Sets display language.
Write Protect Protects programs or menu options from modification.
Time / Date Sets time and date.
Position Description Range
Pipetting
Height Over-
ride
Enables the pipetting height override. The active
pipetting height can be overcome by pushing down on
the control unit for a short time. Press OK to toggle
between On and Off.
/
(On/Off)

MINI 96 – Operating instructions V00 Installation
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3.4.2 Preferences
Preferences customizes your system parameters. Use the Touch wheel to select an
option and press OK to access. The desired settings are saved directly.
Preferences Description Range
Deck Bright-
ness
Sets brightness of the deck light from off, 1 (weak) to 10
(bright).
Off
1-10
Dim Time Sets the time to enter the dim mode. 2-30 min
LED Bar
Brightness
Sets brightness of the LED bars on the device from off, 1
(weak) to 10 (bright).
Off
1-10
Display
Brightness
Sets brightness of the display from 1 (weak) to 10
(bright).
1-10
Handle
Sensitivity
Sets the movement sensitivity of the pipetting unit from 1
(low) to 10 (high). 1-10
Sound Select an option and press OK to activate or deactivate
beep tones:
•Step Complete: at the end of a program step
•Program Complete: at program completion
•PURGE Key: when PURGE is pressed.
•Messages: when a message appears.
•Error Messages: when an error message appears or
when illegal data entry is attempted.
•Touch Wheel: When spinning the Touch wheel.
•Last dispense: before the last dispense in Repeat
Dispense and Variable Dispense.
/
(On/Off)
Main Menu Define which programs are displayed in the Main Menu.
Select the most often used programs and press OK to
change their status to either On or Off. Programs marked
On are displayed in the Main Menu, and all others under
More Programs.
/
(On/Off)
Touch
Wheel
Adjust your Touch wheel spin sensitivity. Low, Medium,
High

MINI 96 – Operating instructions V00 Installation
16 INTEGRA Biosciences AG
NOTE
Extending the volume range is not recommended. Full functionality and
specified accuracy/precision cannot be guaranteed.
Pipetting Select an option and press OK.
•PURGE Speed: Choose the desired purge speed.
•BlowIn Delay: Choose a timed delay between the
blowout and the blowin at the end of a dispense, if no
two step blowout is performed, see “4.3.3 Blowout/
blowin modes” on page 23.
•Extend Volume: For pipetting below the volume range
specified:
50 μl pipette: (1.0)–2–50 μl
125 μl pipette: (2.0)–5–125 μl
300 μl pipette: (5.0)–10–300–(310) μl
1250 μl pipette: (25)–50–1250 μl
The volumes in brackets refer to extended volumes,
e.g. extend the minimal pipetting volume of a 125 μl
pipette from 5 μl to 2 μl.
•Speed Table: Change the default pipetting speed (see
7.5) of any speed step. Press to save.
•Pace in Custom: Allows for continuous dispensing by
pressing and holding RUN during consecutive dis-
penses. To activate this feature, set the time gap
between dispenses.
1-10
None/
0.5-5.0 s
/
(On/Off)
μl/s
None/1-10
(slow-fast)

MINI 96 – Operating instructions V00 Installation
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3.4.3 Calibration & Services
These options enable you to set calibration features and review service history.
Calibration
& Services Description Range
Calibration Allows for re-calibration of MINI 96 to restore accuracy.
The calibration factors for Pipet and Repeat type are
displayed. To edit the calibration volumes, press .
•Target Volume: This is the volume you are interested
in using for the calibration.
•Actual Volume: This is the measured volume obtained
when dispensing the target volume.
•Current Factor: Displays the factor currently in use.
This factor should be the same as specified on the lat-
est calibration certificate.
Enter the Actual Volume and press OK. Press Start to
calculate and save the new Current Factor. Press 
Reset to set the correction factor back to the original
Factory Factor 1.0000.
-
Calibration
Reminder
Sets a calibration reminder based on a number of
pipetting cycles. When the calibration reminder is
displayed, press any key to confirm. However, the
reminder will reappear every time the pipette is turned on
until you change the reminder time or use the reset
option.
•Reminder Time: Press OK to turn the reminder timer
On or Off. Select Days to define the reminder time.
•Reminder Cycles: Use the Touch wheel to set a
reminder interval for calibration (time in thousands of
cycles).
Remind in: Displays the residual amount of day/cycles
before calibration is required.
Reset: Resets the timer back to the original factory
interval. Press to save the defined interval.
/
(On/Off)
1 - 365 days
1 - 240 K
Service
History
Displays notes of any service that took place on the
MINI 96 listed newest entry first.
-

MINI 96 – Operating instructions V00 Installation
18 INTEGRA Biosciences AG
3.4.4 Communications
The latest firmware for MINI 96 can be loaded from a PC via a USB-C to USB
communication cable.
3.4.5 Device Information
3.4.6 Park Head
3.4.7 Language
Communi-
cations Description
USB Connect the USB-C cable between MINI 96 and a PC. Press OK to begin
bi-directional communication. To exit the communications mode turn off
MINI 96.
Device
Information Description
MINI 96 Information about your MINI 96, such as volume range, owner, serial
number, head number, firmware (FW) version and other device details are
displayed.
Edit Owner: Press to enter the user name for your pipette. Use the
Touch wheel to highlight a character and press OK. You can press to
Delete the last character entered. After entering the desired text, press 
to save.
Park Head Description
Park Head Fixes the pipetting unit for safe shipping, for example back to INTEGRA
for calibration. Make sure GripTips are ejected and the deck is cleared.
Press RUN to start the park routine. The pipette moves to park position
and can then be turned off.
Language Description
Language You can choose the language in which all screens are displayed. Scroll to
the desired language, press OK and to save.

MINI 96 – Operating instructions V00 Installation
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3.4.8 Write protect
Select this option to protect programs and menu options from inadvertent modification.
The pipetting programs can still be used.
Keep the password in a safe place. Should you lose your password, contact
INTEGRA Biosciences for assistance in retrieving it.
Write
protect Description Range
Select an option and press OK to switch protection on or
off:
•Standard Programs
•Custom Programs
•Calibration
•Settings
•Password Protection: Protect the access to the write
protect menu by selecting “”.
•Edit Password: if password protect is switched on. To
enter a password use the Touch wheel to highlight a
character and press OK. Press to save the pass-
word. The password must be entered before you can
access the write protect menu.
/
(On/Off)

MINI 96 – Operating instructions V00 Operation
20 INTEGRA Biosciences AG
4 Operation
4.1 Turn on/off the device
Turn on:
CAUTION
Remove hands from Control unit (6) at switch on and during homing.
Press the Main switch (11) to turn on. You are prompted to press RUN (19) to perform a
vertical homing routine. After homing the Main Menu is displayed.
Turn off:
Press the Main switch to turn off.
NOTE
MINI 96 automatically goes into stand-by after 30 minutes of inactivity.
Press OK to continue.
4.2 Attaching and removing GripTips
CAUTION
Use only GripTips in boxes designated for benchtop pipetting systems, see
“8 Accessories and consumables” on page 52 that fit to the Base stage and
allow supported Griptip loading.
To prevent contamination of MINI 96 pipetting unit, it is recommended to use
filtered GripTips only.
4.2.1 Loading tips from a rack with 96 tips
Insert the Base stage on the Deck (2). The graphic “96” must point upwards and match
the tip rack pattern (“96” for 300 μl and 1250 μl devices). Put a tip rack on the Base stage.
Hold the Control unit (6) and lower the Pipetting unit (8) down onto the tip rack until you
are prompted to press OK (16) to start automated tip loading.
4.2.2 Loading tips from a rack with 384 tips
12.5 μl and 125 μl tips are available in racks of 384 tips. With a 96 channel head you can
load tips four times from these racks, drastically reducing plastic waste. Insert the Base
stage on the Deck with side “384” pointing upwards. Put a tip rack on the Base stage.
Move the tip rack to one corner, e.g. to the front left to load
the back left quadrant. Hold the Control unit (6) and
lower the pipetting unit down onto the tip rack and load
tips by pressing OK (16).
Load the other quadrants in any order.