Integra PIPETBOY acu 2 User manual

PIPETBOY acu 2 Operating instructions
466079_V17

i/iii INTEGRA Biosciences AG
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
comply with:
Description Models
PIPETBOY acu 2 155000, 155015, 155016, 155017, 155018, 155019, 155022,
155023, 155024
Accessories 153210, 153211, 153214, 153216, 155066
International Scope
UN 38.3 Lithium battery testing requirements
EU Directives Scope Date effective
2014/35/EU Low voltage directive (LVD) 20.04.2016
2014/30/EU Electromagnetic compatibility (EMC) 20.04.2016
2012/19/EC
Waste electrical and electronic equipment (WEEE) 14.02.2014
2011/65/EC Restriction of hazardous substances (RoHS) 03.01.2013
2006/66/EC Battery directive 26.09.2008
EU Regulations Scope Date effective
1907/2006
Registration, evaluation, authorisation and
restriction of chemicals (REACH)
01.06.2007
2019/1782 External power supply efficiency 01.04.2020
1103/2010 Capacity labelling of portable batteries 30.11.2010
EU Standards Scope
EN 9001:2015 Quality Management
EN 61010-1:2020 Safety general laboratory equipment
EN 61326-1:2013 Electromagnetic compatibility laboratory equipment
EN 60950-1:2013 Safety information technology equipment
EN 62368-1:2021 Safety information technology equipment
EN 62133-2:2017 Batteries containing non-acid electrolytes

www.integra-biosciences.com ii/iii
PIPETBOY acu 2 – Declaration of conformity
GBR Regulations Scope Date effective
S.I. 2016/1101
Electrical equipment safety 08.12.2016
S.I. 2016/1091 Electromagnetic compatibility (EMC) 08.12.2016
S.I: 2008/2164 Batteries and accumulators regulations 26.09.2008
S.I. 2013/3113
Waste electrical and electronic equipment (WEEE) 01.01.2019
S.I. 2012/3032 Restriction of hazardous substances (RoHS) 02.01.2013
GBR Standards Scope
BS 61010-1:2010 Safety general laboratory equipment
BS 62368-1:2020 Safety information technology equipment
BS 63000:2018 Restriction of hazardous substances (RoHS)
USA Regulations Scope Date effective
47 CFR Part 15 (FCC)
Electromagnetic compatibility (EMC)
10 CFR Part 430 External power supply efficiency (CEC VI)
17 CFR Parts 240 & 249bDodd frank “Conflict minerals”
27 CCR Parts 25102-
27001
Proposition 65: The safe drinking water and
toxic enforcement act
20 CCR Parts 1601-1608
CEC BCS, Battery charging efficiency 01.01.2017
TSCA 40 CFR Part 751 Toxic substances control act
USA Standards Scope
UL 61010-1:2012 Safety general laboratory equipment
CAN Standards Scope
CSA-C22.2
No. 61010-1
Safety general laboratory equipment

iii/iii INTEGRA Biosciences AG
PIPETBOY acu 2 – Declaration of conformity
Zizers, April 11, 2022
CHN Regulations Scope Date effective
AQSIQ Order 5 /2001 (CCC) safety and EMC requirements for
electrical equipment
01.08.2003
Order 32/2016 Restriction of hazardous substances (RoHS) 01.07.2016
CHN Standards Scope
GB4943.1-2011 Information technology equipment safety
GB9254-2008 Information technology equipment radio
disturbance
GB17625.1-2012 EMC limits for harmonic current emissions
GB31241-2014 Safety for Lithium-ion batteries
SJ/T 11364-2014 Restriction of hazardous substances (RoHS)
JPN Regulations Scope Date effective
PSE (Denan) Law
Electrical appliance and material safety law 01.01.2014
EAC Технический регламент Таможенного союза
ТР ТС 004/2011 О
безопасности низковольтного оборудования
ТР ТС 020/2011 Электромагнитная совместимость технических средств
Urs Hartmann
CEO
Daniel Bächi
Head of Corporate Quality

PIPETBOY acu 2 - Operating instructions V17
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Table of Contents
1 Introduction ................................................................................................................ 6
2 Description of the device............................................................................................ 8
3 Installation ...................................................................................................................9
4 Operation ................................................................................................................. 10
5 Maintenance............................................................................................................. 13
6 Technical Data ......................................................................................................... 14
7 Accessories.............................................................................................................. 16
Imprint
© 2022 INTEGRA Biosciences AG
This operating instruction manual applies to serial number 1350 000 or higher of
PIPETBOY acu 2 / PIPETGIRL.
Manufacturer and customer service
Your local INTEGRA Biosciences representative, further information, and operating
instructions in other languages can be found at www.integra-biosciences.com or are
available on request [email protected]m.
INTEGRA Biosciences AG
Tardisstrasse 201
CH-7205 Zizers, Switzerland
T [phone]
INTEGRA Biosciences Corp.
22 Friars Drive
Hudson, NH 03051, USA
T [phone]
INTEGRA Biosciences SAS
8 avenue du Fief
95310 Saint Ouen l'Aumône, FR
T [phone]
INTEGRA Biosciences Deutschland GmbH
An der Amtmannsmühle 1
35444 Biebertal, DE
T [phone]
INTEGRA Biosciences Ltd
2 Rivermead Business Park
Thatcham, Berks, RG19 4EP, UK
T [phone]
INTEGRA Biosciences (Shanghai) Co., Ltd.
Room 1110, No. 515 Huanke Road
Shanghai 201315, CN
T [phone]
INTEGRA Biosciences KK
Higashikanda 1-5-6, Chiyoda-ku
Tokyo, 101-0031, JP
T [phone]

PIPETBOY acu 2 - Operating instructions V17 Introduction
6 INTEGRA Biosciences AG
1 Introduction
1.1 Intended use
PIPETBOY acu 2 is a pipette controller designed for aspirating and dispensing aqueous
solutions with plastic or glass pipettes of 1 to 100 ml volumes. It is intended for
measurement, control and laboratory use. Any use of this instrument in a medical or IVD
setting is under the sole responsibility of the user.
PIPETGIRL is a special model of PIPETBOY acu 2 and provides the same functions as
PIPETBOY acu 2.
1.2 Safety notes
1) Do not use or charge PIPETBOY acu 2 in an atmosphere with danger of explosion.
Also, do not pipette highly flammable liquids such as acetone or ether.
2) When handling dangerous substances, comply with the material safety data sheet
(MSDS) and with all safety guidelines such as the use of protective clothing and safety
goggles. Never point a pipette in anyone’s direction.
3) Avoid pipetting of liquids whose vapours could attack the materials PA (polyamide),
POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrile-rubber), CR (chloroprene),
silicone. Corrosive vapours could also damage metallic parts inside the device.
4) Prolonged exposure of PIPETBOY acu 2 to UV-light can cause discolouration and/or
yellowing of the plastic housing. However, this will not affect the performance of the
device in any way.
5) Only use the original Li-ion battery (part no. 155066) and an original
INTEGRA Biosciences mains adapter and protect it from moisture, otherwise
PIPETBOY acu 2 might be damaged.
6) Old Li-ion batteries may cause a safety risk. We recommend to replace the battery after
3 years of use. Also replace the battery if the charging intervals are unusually short or
if the charging takes much longer than usual (4 hours or more). These are indicators
that the battery has reached the end of its life-cycle.
If a lithium battery is never deep discharged and is always stored and operated in the
recommended temperature range and stored at 40-80% charge level during long
standby periods, it may live much longer than 3 years. If it shows no signs of physical
damage or change, see 5.1, it is a strong indication that you may continue to use the
battery.
7) Li-ion technology bears the risk of thermal runaway and cell rupture if the battery was
damaged. Do not expose the battery to heat (> 60°C) and avoid mechanical stress.
Batteries which were subject to deep discharges may develop internal short circuits,
leading to an increased self-discharge rate and heating during battery charging. This
may also result in thermal runaway and cell rupture.

PIPETBOY acu 2 - Operating instructions V17 Introduction
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8) To extend the battery life-cycle, it is recommended to charge the battery every 2
months if the pipette controller is not used regularly. If the pipette controller is not used
for more than 6 months, remove the battery from the instrument.
Regardless of the listed safety notes, additionally applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

PIPETBOY acu 2 - Operating instructions V17 Description of the device
8 INTEGRA Biosciences AG
2 Description of the device
2.1 Scope of delivery
• PIPETBOY acu 2 device
• 1 rechargeable Li-ion battery
• Mains adapter
• Wall mount
• Hydrophobic sterile filter 0.45 µm (spare)
• Quick Start Guide
2.2 Overview of PIPETBOY acu 2
1 Aspiration button
2 Dispensing button
3 Thumb wheel to set maximum speed
4 Battery charge indicator
5 Socket for mains adapter cable
6 Handle
7 Sterile module complete (=nose piece)
7 Sterile module complete (=nose piece)
7a O-ring
7b Housing of sterile module, upper part
7c Filter rubber
7d Hydrophobic filter
7e Pipette mount
7f Housing of sterile module, lower part
1
2
4
5
7
6
3
c
d
e
f
b
7a

PIPETBOY acu 2 - Operating instructions V17 Installation
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3 Installation
3.1 Charging the battery
A full charge takes 3.5 hours. Before the first use, PIPETBOY acu 2 should be charged
until the battery charge indicator (4) turns green, showing that the battery is full.
When the battery charge indicator (4) starts flashing red, PIPETBOY acu 2 can be used
for around 100 pipetting cycles before shutting down. It should thus be recharged
immediately.
PIPETBOY acu 2 has an integrated protection: it will not overcharge even if it is connected
to power for indefinite time. To avoid unnecessary power consumption, it is recommended
to unplug the power supply when the charge indicator is green. PIPETBOY acu 2 can be
used while it is being charged.
The battery charge indicator provides various information:
3.2 Replacing the battery
3.3 Mounting of the wall mount
The wall mount serves to park PIPETBOY acu 2.
To mount the wall mount, remove the protective foil from the adhesive tape at the back of
the holder. Hold it with the sign <up> facing upwards and press it to the desired place.
Make sure that the surface onto which the wall mount is mounted is smooth, clean and
grease-free. Wait 24 hours before using the wall mount for the first time. Alternatively the
wall mount can be fixed with the included screws.
Battery charge indicator Battery status and information
Flashes red Battery is low. Charging is needed.
Is red and power supply is connected Battery is being charged.
Is green and power supply is connected Battery is fully charged.
Flashes alternately red and green Battery error. Check if the correct type of
battery with correct polarity (+/-) and/or
power supply are used.
1) Move the lid of the battery compartment upwards and
remove it (a).
2) Replace the old battery with an original
INTEGRA Biosciences rechargeable battery (see “7
Accessories” on page 16) and make sure that it is
inserted with the correct polarity (+/-).
3) Close the battery compartment with the lid (a).
a

PIPETBOY acu 2 - Operating instructions V17 Operation
10 INTEGRA Biosciences AG
4 Operation
4.1 Inserting the pipette
WARNING
Do not insert pipettes with force into PIPETBOY acu 2, because they can break
and cause injury, particularly thin pipettes made of glass.
4.2 Pipetting
Press the aspiration button (1) to fill the pipette and the dispensing button (2) to empty it.
The aspiration and dispensing speed can be controlled in two manners:
• Fine speed adjustment by varying the finger pressure on the buttons (1, 2).
• Step-less presetting of the maximum pump speed by turning the thumb wheel (3) to
optimally match the pipette volume (turning to the left = slower pump speed, for small
pipettes; to the right = faster, for large pipettes).
To empty the pipette by gravity force, press the dispensing button only slightly in order to
avoid reaching the trigger point where the pump starts running. Gravity dispensing is used
for “to deliver” (TD) pipettes that are not of the “blow-out” type (blow-out pipettes have two
thin rings or a frosted band around the neck).
PIPETBOY acu 2 is featured with a “TURBO” mode. Plug the mains adapter cable into the
pipette controller and turn the thumb wheel completely to the right for maximal speed.
The silicone pipette mount (7e) has a special conical
channel to guarantee a firm and leak-proof grip of the
pipette independently of its diameter.
Disassemble the nose piece (see “5.1 Cleaning and
servicing” on page 13) and orient the pipette mount:
a) with the large opening facing down for pipettes > 2 ml
(factory setting), or
b) with the small opening facing down for pipettes < 2 ml.
a) b)

PIPETBOY acu 2 – Operating instructions V17 Operation
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4.3 Troubleshooting Problem Probable cause Remedy Pipette drips (leak in the system).
Pipette is damaged or not fully inserted in the nose piece (7).
Reinsert a new pipette and push it all the way into the nose piece. Make sure that the pipette mount orientation is correct (see section 4.1).
The inside of the pipette mount (7e) is
damaged resulting in insufficient sealing of the pipette neck.
Replace the pipette mount (#151 020).
The filter rubber (7c) or the filter (7d) in the
nose piece are damaged or missing causing a leak.
Replace the filter rubber (#153225) and/or the filter (Part No. see “7 Accessories” on page 16).
Reduced aspiration efficiency or no liquid aspiration.
The filter (7d) is wet or dirty. Replace filter.
The nose piece (7) is not tight. Tighten the nose piece, or replace defective parts.
The battery is discharged (battery charge indicator flashes red)
Charge the battery.
The battery is missing. Insert the battery or connect the instrument to the
mains adapter.
The battery is defective. Replace the battery. The battery is wrongly inserted. Insert correctly, note polarity (+) and (-).
Reduced operating time with fully charged battery.
The battery is worn. Replace the battery. Wrong battery type is inserted. Use only original Li-ion battery (#155 066).
Extremely long charging time of battery.
Wrong mains adapter is used. Use only original mains adapter (see “7
Accessories” on page 16).
Extremely short charging and operating time.
Wrong battery type is inserted. Use only original Li-ion battery (#155066).

PIPETBOY acu 2 – Operating instructions V17 Operation
12 INTEGRA Biosciences AG
Battery is not charging. The battery is wrongly inserted. Insert correctly, note polarity (+) and (-).
Wrong battery type is inserted. Use only original Li-ion battery (#155066). Wrong mains adapter is used. Use only original mains adapter (see section 7).
Battery operation not possible.
The battery is wrongly inserted. Insert correctly, note polarity (+) and (-). Wrong battery type is inserted. Use only original Li-ion battery (#155066). The battery is missing. Insert the battery.
Problem Probable cause Remedy

PIPETBOY acu 2 - Operating instructions V17 Maintenance
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5 Maintenance
After maintenance work, perform a leak test to ascertain correct functioning of
PIPETBOY acu 2: liquid should not leak out of a filled pipette before the dispensing button
is pressed.
5.1 Cleaning and servicing
PIPETBOY acu 2 can be cleaned with a cloth moistened with soapy water or with a 70 %
ethanol.
Disassembly of the nose piece:
It is recommended to change the hydrophobic filter (7d) every three months. Should the
filter get wetted or soiled, it has to be changed immediately. The filter must be oriented
with the blue (0.45 µl) / red (0.2 µl) side facing upwards towards PIPETBOY acu 2.
Annual inspection: If you operate the battery beyond the recommended 3 year period,
visually check the battery for signs of damage, e.g. discoloration, unexpected stains,
shrinking of the tube wrapping.
5.2 Decontamination
The nose piece housing (7b, 7f), the pipette mount (7e) and the filter rubber (7c) can be
autoclaved at 121 °C, 1 bar overpressure for 20 minutes. Silicone may become brittle after
extensive autoclaving. Replace the pipette mount and filter rubber if they are damaged.
If the housing of the PIPETBOY acu 2 have been in contact with biohazardous material,
it must be decontaminated in accordance to good laboratory practice. Do not spray directly
on the instrument but use a lint-free cloth, lightly soaked with a disinfectant and wipe dry
directly after decontamination. Never use acetone or other solvents! Follow the
instructions provided by the disinfectant manufacturer.
The device may be decontaminated with H2O2gas (maximal concentration 35 %) for 60
minutes.
Unscrew the nose piece (7) from the handle by turning it
counter clockwise. Hold the upper part of nose piece (7b),
press the lower part (7f) firmly against the upper part (7b)
and turn it counter clockwise (left). The lower part of nose
piece housing (7f) will disengage after about 1/8th of a
turn.
Remove the pipette mount (7e), the filter (7d) and the
filter rubber (7c), if required.

PIPETBOY acu 2 - Operating instructions V17 Technical Data
14 INTEGRA Biosciences AG
5.3 Equipment disposal
PIPETBOY acu 2 device must not be disposed of with unsorted municipal
waste. Do not dispose of the device in a fire.
PIPETBOY acu 2 contains a Li-ion battery. Do not modify the battery in any
way. Dispose of the PIPETBOY acu 2 device and the battery separately in
accordance with the laws and regulations in your area governing disposal of
devices containing Li-ion batteries.
In certain regions and countries, e.g. in all EU member states, the distributor is obliged to
take back this product free of charge at the end of life. Please contact your local distributor
for more details.
6 Technical Data
6.1 Specifications
Pipetting speed max. 13.5 ml/s (with a 50 ml serological pipette)
Battery Type: rechargeable, Li-ion, min. 500 mAh
Typical charging time: 3.5 hours
Charging cycles: 500–1000 (when charging as indicated)
Running time: at least 5500 cycles of aspiration and
dispensing of 25 ml.
Electricity supply Mains adapter input: 100–240 VAC, 50/60 Hz
Device Input: 16–19 VDC, 3.1 W
Materials Housing: PA
Nose piece housing: POM
Pipette mount: Silicone
Filter rubber: Silicone
Dimensions (H x W x D) 125 x 130 x 35 mm
Weight 195 g
Ambient conditions Operation: 5–40°C, max. 80% RH
Storage: -10–50°C, max. 95% RH

PIPETBOY acu 2 - Operating instructions V17 Technical Data
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6.2 Chemical compatibility
The table below lists PIPETBOY acu 2 parts that come into contact with the aspirated
liquid or its aerosols and vapors, and rates the compatibility of these parts to a few of the
chemicals commonly used in laboratories. To determine the compatibility of a component
to a chemical not listed in the table, please consult one of the several tables available on
the internet. Note that the rating refers to soaking in the concentrated chemical; however,
more relevant here is the attenuated effect resulting from vapors and the diluted chemical.
It is recommended to test the compatibility of relevant components to a specific chemical
prior to extensive use.
INTEGRA Biosciences does not warrant that the information in the table is accurate or
complete and that any material is suitable for any purpose.
Chemical compatibility chart
Compatibility ratings:
A = Good: no or minor effects.
B = Fair: moderate effects, not recommended for continuous use.
C = Critical: not recommended, suitability to be determined by test.
Parts Materials
JAVEL (e.g. NaClO)
Acetic acid
Ethanol
Isopropyl alcohol
NaCl saturated
Sodium hydroxide (50%)
Sodium acetate
(3M, pH 5.2)
Hydrochloric acid (20%)
Chloroform
Acetone
Handle PA A C B A A A A C C A
Nose piece housing POM C C A A A A A B A A
Pipette mount,
Filter rubber, tubings
Silicone A B A A A A C A C C
Internal parts
(e. g. pump)
FPM AAAAAC C AAC
NBR ABBAAA A ACC
CR AAAAAA A ABA
Metal CCAABC A CAA

PIPETBOY acu 2 - Operating instructions V17 Accessories
16 INTEGRA Biosciences AG
7 Accessories
Accessories Part No.
Stand for
PIPETBOY
for a well organized work area, to park PIPETBOY acu 2 with
inserted pipette
155065
Wall mount for holding PIPETBOY acu 2 on the wall 155521
Mains adapter
(100-240 VAC,
50/60 Hz)
EU version 153210
US/JP version 153211
UK version 153214
AU version 153216
Consumables Part No.
Sterile module
(nose piece)
complete (7)
turquoise for PIPETBOY acu 2 classic and transparent;
with filter 0.45 µm, unsterile
155025
classic (grey) for colored PIPETBOY acu 2 and PIPETGIRL;
with filter 0.45 µm, unsterile
155070
Sterile module
(nose piece)
housing (7b,7f)
turquoise, for PIPETBOY acu 2 classic and transparent 155230
classic (grey), for colored PIPETBOY acu 2 and PIPETGIRL 155060
Filter 0.45 µm
(7d)
sterile/unsterile, for the country specific part numbers,
please refer to our website
Filter 0.2 µm
(7d)
sterile/unsterile, for the country specific part numbers,
please refer to our website
Pipette mount
(7e)
silicone, for holding pipette in the sterile module (nose piece) 151020
Filter rubber (7c) silicone, for holding the filter in the sterile module (nose
piece)
153225
O-ring (7a) 153235
Battery
compartment lid
classic (grey) 155203
green 155160
red 155161
blue 155162
transparent 155163
purple 155164
pink (PIPETGIRL) / Pink Sunrise 155166
Agave 155167
Ocean Dream 155168

PIPETBOY acu 2 - Operating instructions V17 Accessories
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WARNING
Battery Li-ion for PIPETBOY acu 2 / PIPETGIRL
(with serial number >=1350001)
155066
Consumables Part No.
Only use the battery Li-ion for PIPETBOY acu 2
(serial number >=1350001; LED is white when inactive,
red or green when active)!
The NiMH battery for PIPETBOY acu is not compatible!
serial number