Integra Evolve 3011 User manual

EVOLVE Manual Pipettes Operating instructions
900-00010-00_V00

Declaration of conformity
INTEGRA Biosciences Corp. – Hudson, NH, USA
declares on its own responsibility that the devices
comply with:
Hudson, New Hampshire, USA - March 15, 2016
Description Models
EVOLVE Manual
Pipettes
3011, 3012, 3013, 3015, 3016, 3018, 3019, 3022, 3024, 3025,
3026, 3032, 3034, 3035, 3036
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Restriction of Hazardous Substances 2011/65/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals
(REACH)
1907/2006
Piston-operated volumetric apparatus - Part 2: Piston pipettes ISO 8655-2
George Kalmakis
VP Operations

EVOLVE Manual Pipettes – Operating instructions V00
www.integra-biosciences.com 3
Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................5
1.3 Safety notes .....................................................................................6
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................7
2.2 Overview of the EVOLVE Manual Pipettes ......................................7
Chapter 3 Installation
3.1 Optional color coded plunger cap.....................................................8
3.2 Changing the Plunger Spring ...........................................................8
Chapter 4 Operation
4.1 Setting the volume..........................................................................10
4.2 Attaching and removing GripTip pipette tips ..................................10
4.3 Pipetting .........................................................................................11
4.3.1 Aspirating and Dispensing ..................................................11
4.3.2 Reverse mode.....................................................................12
4.3.3 Proper pipetting techniques ................................................13
4.4 Troubleshooting..............................................................................14

EVOLVE Manual Pipettes – Operating instructions V00
4 INTEGRA Biosciences AG
Chapter 5 Maintenance
5.1 Cleaning .........................................................................................15
5.2 Single channel lower end disassembly ..........................................15
5.2.1 Disassembly........................................................................15
5.2.2 Reassembly ........................................................................18
5.3 Sterilization.....................................................................................20
5.3.1 Autoclaving the disassembled components........................20
5.4 Servicing.........................................................................................21
5.4.1 Shipping to INTEGRA Biosciences.....................................21
5.4.2 Changing O-rings of tip fittings............................................21
5.4.3 Lubrication ..........................................................................22
5.5 Calibration ......................................................................................23
5.5.1 Materials .............................................................................23
5.5.2 Definitions ...........................................................................23
5.5.3 Test conditions and environment ........................................24
5.5.4 Pre-wetting of tips ...............................................................24
5.5.5 Leak test .............................................................................25
5.5.6 Obtaining the actual volume ...............................................26
5.5.7 Calculation of accuracy and precision ................................27
5.5.8 Adjusting EVOLVE Manual Pipettes ...................................28
5.6 Equipment disposal ........................................................................29
Chapter 6 Technical Data
6.1 Environmental conditions ...............................................................30
6.2 Pipette specifications......................................................................30
6.3 Z Correction Factors.......................................................................32
Chapter 7 Accessories and Consumables
7.1 Accessories ....................................................................................33
7.2 Consumables .................................................................................33
Imprint................................................................................................... 36

EVOLVE Manual Pipettes – Operating instructions V00 Introduction
www.integra-biosciences.com 5
1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the EVOLVE Manual Pipettes. This chapter informs about the
symbols used in these operating instructions, the intended use of the EVOLVE Manual
Pipettes and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
WARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
CAUTION
This symbol cautions against potential material damage. Follow the
instructions.
NOTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
1.2 Intended use
EVOLVE Manual Pipettes are intended to be used in a laboratory for aspirating and
dispensing liquids in the volume range of 0.2–5000 µl using GripTip pipette tips.

EVOLVE Manual Pipettes – Operating instructions V00 Introduction
6 INTEGRA Biosciences AG
1.3 Safety notes
EVOLVE Manual Pipettes comply to the recognized safety regulations and is safe to
operate. EVOLVE Manual Pipettes can only be operated when in perfect condition and
while observing these operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the EVOLVE Manual Pipettes must have read
and understood these operating instructions, and particularly, the safety notes, or must
have been instructed by supervisors so that safe operation of the device is guaranteed.
Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after-
sales service member.
Parts may be replaced with original INTEGRA Biosciences parts only.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.
WARNING
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
Do not immerse the EVOLVE Manual Pipettes in liquid. The fluid can damage
internal parts. Avoid pipetting of liquids whose vapors could attack the
materials PA (polyamide), POM (polyoxymethylene), FPM (fluor-rubber),
NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive vapors could also
damage metallic parts inside the device.
NOTE
Prolonged exposure of the EVOLVE Manual Pipettes to UV-light can cause
discoloration and/or yellowing of the pipette housing. However, this will not
affect the performance of the device in any way.

EVOLVE Manual Pipettes – Operating instructions V00 Description of the device
www.integra-biosciences.com 7
2 Description of the device
2.1 Scope of delivery
• EVOLVE Manual Pipette
• Multipurpose Tool
• Replacement Plunger Cap
• Quick Start Guide
• Replacement O-rings (for 200 μl, 1000 μl and 5000 μl models)
2.2 Overview of the EVOLVE Manual Pipettes
1Plunger Cap
2Plunger, to pipette
3Tip Ejector
4Finger Hook
5Adjustment Dials, to set volume
6Volume Window
The color of the volume indicator label matches
GripTip box insert
7Ejector Sleeve
8Tip Fitting
1
2
4
6
3
5
7
8

EVOLVE Manual Pipettes – Operating instructions V00 Installation
8 INTEGRA Biosciences AG
3 Installation
3.1 Optional color coded plunger cap
If desired, replace black plunger cap with optional color coded plunger cap.
3.2 Changing the Plunger Spring
The plunger spring installed inside the pipette is the light spring option. You can exchange
with a heavier plunger spring to achieve more control of the plunger movement.
1) Unscrew the Cylinder Assembly (a) and
separate the Piston Assembly (b, models 2–
200 µl) or Piston (model 1000 µl) from the upper
part of the pipette (c). It is held in place by a small
magnet.
NOTE
5000 µl piston and cylinder assembly is
removed as one piece.
2) Set the pipette to the maximum setting. The
maximum setting is higher than the maximum
volume.
3) Put the two teeth of the Multipurpose Tool (d)
vertically into the slots of the black opening inside
at the bottom of the pipette‘s body (c). Rotate
counterclockwise until the plunger spring
assembly (e) is released.
a
b
c
d

EVOLVE Manual Pipettes – Operating instructions V00 Installation
www.integra-biosciences.com 9
CAUTION
Validate or calibrate your pipette after reassembly.
4) To install the optional spring assembly, screw the
plunger spring assembly (e) clockwise into the
upper part of the pipette using the Multipurpose
Tool.
5) Re-attach the Piston assembly/Piston. Screw
clockwise to attach the Cylinder Assembly (a) to
the body of the pipette.
e

EVOLVE Manual Pipettes – Operating instructions V00 Operation
10 INTEGRA Biosciences AG
4 Operation
4.1 Setting the volume
4.2 Attaching and removing GripTip pipette tips
CAUTION
To ensure optimal performance of your EVOLVE Manual Pipettes always use
GripTips of correct size which have been designated for the use with the
EVOLVE Manual Pipettes, see “7.2 Consumables” on page 33.
The unique GripTip system of the EVOLVE Manual Pipettes reduces attachment and
ejection forces, ensures a perfect fitting to prevent the tips from falling off and to provide
a perfect seal. On a multichannel pipette all tips sit on the same height.
Attach the tips:
When loading tip(s), press the pipette into the appropriate GripTip(s) until you hear and
feel a click indicating that a seal has been achieved. Once you feel the click, stop applying
pressure. When loading GripTip pipette tips using a multichannel pipette, slowly rock the
pipette from one side to the other side to ensure the proper seal is achieved.
Discard your used tips:
If liquid is in the tips, empty them by pressing down the Plunger (2) to the second stop
(see section 4.3). Tips are easily removed by pressing the Tip Ejector (3).
1) To set the desired pipetting volume press down the Plunger (a)
and rotate slightly clockwise to unlock the volume dials.
2) Spin the Adjustment Dials (b) until the desired volume is
shown in the Volume Window.
3) Slightly rotate and slowly release the Plunger (a) to lock the
volume.
a
b
A rim inside the GripTips snaps over the multi-lobes and ensures
firm attachment of the tips.
A shoulder provides a positive stop to prevent over tightening of the
tips. Without hammering, the tip is either on or off but nothing in
between.
O-ring provides forgiving and robust seal surface for the pipette tip.

EVOLVE Manual Pipettes – Operating instructions V00 Operation
www.integra-biosciences.com 11
4.3 Pipetting
The Plunger can be pushed down to two different stops:
For detailed recommendations for pipetting see also “4.3.3 Proper pipetting techniques”
on page 13.
4.3.1 Aspirating and Dispensing
To aspirate and dispense liquid perform the following steps:
1) Top position: Varies depending on volume setting.
2) First stop: Fixed calibrated position for aspirating and
dispensing.
3) Second Stop: To blowout the residual liquid.
Aspirate
1) With GripTip(s) attached, push down the Plunger (2) to the first
stop.
2) Place GripTips in liquid. Hold the pipette vertically or in an angle
of max. 20° throughout the entire pipetting process and immerse
the GripTip(s) just below the surface of the liquid (2–3 mm).
3) Slowly return Plunger to the top position. For higher volumes
(>200 μl), follow the liquid level by moving the pipette down.
4) Remove the GripTip(s) from liquid.
Dispense
5) To dispense the liquid, push the Plunger (2) down to the first
stop.
6) If there is any residual liquid in tip, a blowout should be
performed by pressing the Plunger to the second stop.
7) Remove the GripTips from vessel and release Plunger.
1
2
3

EVOLVE Manual Pipettes – Operating instructions V00 Operation
12 INTEGRA Biosciences AG
4.3.2 Reverse mode
Viscous samples should be aspirated and dispensed at slower speeds and in reverse
pipetting mode:
Aspirate
1) Fully push down the Plunger (2) to the second stop.
2) Immerse the GripTip(s) in the liquid, let the Plunger slowly
release to the upper position and wait shortly until the liquid is
fully aspirated.
Dispense
3) To dispense, press the Plunger (2) slowly down to the first stop
and wait a few seconds to dispense the liquid.
4) Remove the GripTip(s) from the target vessel.
5) To expel the excess liquid press the Plunger to the second
stop.

EVOLVE Manual Pipettes – Operating instructions V00 Operation
www.integra-biosciences.com 13
4.3.3 Proper pipetting techniques
INTEGRA Biosciences recommends the following techniques for enhancing pipetting
results. These techniques are consistent with ISO standard 8655-2.
• It is best to immerse the GripTips just below the surface of the liquid (2–3 mm) to allow
the desired volume to be aspirated. If the tip is immersed too deep, the risk of carry-
over is increased due to liquid drops clinging to the outer surface of the tip.
• Press and release the plunger with consistent speed and smoothness.
• Always prewet GripTips. After loading tips onto your pipette, aspirate and dispense the
full volume 2-3 times to coat the inside of pipet tips. Pre-wetting ensures that the liquid
and air inside the tips are at equal temperature and the dead air space is humidified.
Neglecting to perform a pre-wet can result in a smaller delivery volume in the first few
dispenses.
• EVOLVE Manual Pipettes are air displacement pipettes. To properly dispense liquids,
ensure that the pipette tip is at a 0–20° angle. After each dispense, you must touch
GripTips against the vessel wall or liquid. This process is referred to as “touching off”
or “tip touch” and prevents liquid from clinging to the pipette tips.
• Air displacement pipettes show the best performance between 35% and 100% of the
nominal volume.
• Viscous samples should be aspirated and dispensed at slowest speeds to ensure
accurate pipetting. In addition, pipetting should be performed in a reverse mode.
• For pipetting liquids with high vapor pressures (such as methanol or ethanol), use rel-
atively fast pipetting speeds and avoid prolonged pauses after aspiration.
• Calibrate based on fluid type. EVOLVE Manual Pipettes are tested and calibrated at
the factory for use with distilled water at room temperature. It may be necessary to re-
calibrate your EVOLVE Manual Pipettes if the liquid to be used has different physical
properties (specific gravity and vapor pressure) than water.
WARNING
Avoid pipetting for extended periods. To minimize the risk of repetitive strain
injury, include pauses of several minutes.
Use filtered GripTips to pipette corrosive or biohazardous liquids. The filter
prevents vapors and aerosols from corroding or contaminating the cylinder
assembly.

EVOLVE Manual Pipettes – Operating instructions V00 Operation
14 INTEGRA Biosciences AG
4.4 Troubleshooting
Problem Probable cause Remedy
Leaking. • Tip incorrectly attached.
• Foreign particles between tip
and tip fitting.
• Damaged red O-ring.
• Attach a new tip.
• Clean tip fitting. Attach new tips.
• Change the O-ring, see 5.4.2.
• If leak persists, contact service.
Dispense results
are inaccurate.
• Unsuitable calibration for
liquid class.
• Improper pipetting
techniques.
• Recalibrate with the liquids in
question.
• Adjust aspiration and dispense
speed depending on liquid:
- High viscosity liquids may
require calibration.
- High vapor pressure liquids
may require pre-wetting.
- Refer to proper pipetting
techniques section 4.3.3
Not dispensing/
aspirating.
• Piston stuck or not
connected.
• Internal seal is damaged.
• Contact service.
Droplets on the
tips.
• Temperature of liquid differs
from that of air inside the tips.
• Liquid of low viscosity and
high vapor pressure.
• Touch off was not performed.
• Pre-wet tips up to 3 times.
• Increase dispensing speed.
• Perform a touch-off.

EVOLVE Manual Pipettes – Operating instructions V00 Maintenance
www.integra-biosciences.com 15
5 Maintenance
5.1 Cleaning
The materials used on the exterior of the EVOLVE Manual Pipettes support regular
cleaning intervals. Clean the external components with a lint-free cloth lightly soaked with
mild soap solution in distilled water or with a 70 % dilution of Isopropyl or Ethanol. Never
use acetone or other solvents.
WARNING
Do not immerse the entire pipette into a cleaning solution or spray cleaning
solution directly onto the exterior body of the pipette.
If the pipette is exposed to corrosive vapors, e.g. from concentrated acids, or if liquid
enters the internals, disassemble the lower part of the pipette and clean the internal parts
with distilled water or with a 70 % dilution of Isopropyl or Ethanol. Follow the instructions
below for disassembly, also necessary for trouble shooting and autoclaving.
5.2 Single channel lower end disassembly
5.2.1 Disassembly
Disassemble the lower part of the single channel pipette as follows:
Models of all sizes:
1) Unscrew counterclockwise to remove the
Cylinder Assembly (a) from the pipette.
2) Slide the Cylinder Assembly (a) down from the
pipette body. This exposes the Piston Assembly
(b, models 2–200 µl) or Piston with mounted
Cup Seal (m, model 1000 µl).
a
b

EVOLVE Manual Pipettes – Operating instructions V00 Maintenance
16 INTEGRA Biosciences AG
Models 2–20 µl:
3) Slide the following components from the Piston (h):
• O-Ring (black, g) and Seal (white, f) Assembly
•Lower Seal Retainer (white, e)
•Seal Retainer Spring (d)
•Seal Retainer (white, c)
Set these components aside or place them in an
autoclave pouch.
Models 100 µl and 200 µl:
3) Slide the following components from the Piston (h):
• O-Ring (black, g) and Seal (white, f) Assembly
•Seal Retainer Spring (d)
•Seal Retainer (black or white, c)
Set these components aside or place them in an
autoclave pouch.
100 µl 200 µl
Models 2–1000 µl:
4) Separate the Piston (h) from the upper part of the
pipette. The Piston is held in place by a small
magnet.
f
c
d
h
e
g
f
d
c
h
g
f
d
c
h
g
h

EVOLVE Manual Pipettes – Operating instructions V00 Maintenance
www.integra-biosciences.com 17
Models of all sizes:
6) Remove the Ejector Spring (j).
7) Set all components of the lower part aside or place them in an autoclave pouch.
Cylinder Assemblies (a): Models 2–1000 µl:
5) With the Cylinder Assembly (a) in hand, turn
counterclockwise to remove the black Snap Ring
(i). Pullout the black Cylinder (k) from the white
Ejector Sleeve (l).
Model 5000 µl:
5) Put your fingernails into the gap of the white
Ejector Sleeve (l) and pull off the Ejector Sleeve
Cap (n). With the black Cylinder (k) in hand
unscrew the upper part. Remove the Piston (h)
with mounted Cup Seal (m).
2–1000 µl 5000 µl
k
j
i
l
h
l
n

EVOLVE Manual Pipettes – Operating instructions V00 Maintenance
18 INTEGRA Biosciences AG
5.2.2 Reassembly
Before reassembling the pipette, check each component for lint or dust particles. It is
recommended to replace the O-Ring (black, g) and Seal (white, f) or Cup Seal (m).
1) Lightly lubricate the Piston, O-Rings and Seals (see “5.4.3 Lubrication” on page 22).
Models 2 µl–1000 µl:
2) Position the Piston (h) at the small magnet to connect the Piston to the upper part of
the pipette.
Models 2 µl– 200 µl:
3) Slide the Seal Retainer (c) onto the Piston and up into the pipette. The end of the Seal
Retainer that has an extended edge (lip) faces the upper part of the pipette.
4) Slide the Seal Retainer Spring (d) onto the Piston. It rests against the Seal Retainer.
Slide the Lower Seal Retainer (e, 2, 10, 20 µl only) onto the Piston.
5) Slide the lightly lubricated
O-Ring (black, g) and Seal (white, f) Assembly onto the
Piston. The white Seal is closest to the Seal Retainer Spring. Be sure the black O-Ring
is securely slided into the white Seal.
Piston Assemblies (models 2–200 µl) and Pistons with Cup Seal (1000 µl, 5000 µl):
c: Seal
Retainer
d: Seal
Retainer
Spring
e: Lower Seal
Retainer
f: O-Ring
g: Seal
h: Piston
m: Cup Seal
2, 10, 20 µl 100, 200 µl 1000 µl 5000 µl
g
c
d
h
e
f
f
d
c
h
g
h
m
m
h

EVOLVE Manual Pipettes – Operating instructions V00 Maintenance
www.integra-biosciences.com 19
Models of all sizes:
6) Slide the Ejector Spring (j) onto the top of the black Cylinder (k).
Models of all sizes:
10) Screw clockwise to attach the Cylinder Assembly (a) to the body of the pipette.
Perform a leak test (see “5.5.5 Leak test” on page 25) and validate pipetting volumes after
reassembly.
Cylinder Assemblies (a): Models 2 µl– 1000 µl:
2–1000 µl 5000 µl
7) Slide the Cylinder (k) into the white Ejector Sleeve (l).
8) Position the black Snap Ring (i) over the Cylinder (k) at
the top of the Ejector Sleeve (l). Turn the sleeve
clockwise until the Snap Ring snaps into place and
secures the Cylinder in the Cylinder Assembly.
9) Slide the Cylinder Assembly (a) over the Piston (h).
Model 5000 µl:
7) Push the Cup Seal (m) of the Piston (h) into the top of
Cylinder‘s lower part (k) and screw on the upper part of
Cylinder (k).
8) Ensure that the four noses (o) of the Ejector Sleeve Cap
(n) point to the openings (p) of the Ejector Sleeve (l) and
re-attach the cap.
k
j
i
l
n
h
o
p

EVOLVE Manual Pipettes – Operating instructions V00 Maintenance
20 INTEGRA Biosciences AG
5.3 Sterilization
If the surface of the EVOLVE Manual Pipettes have been in contact with biohazardous
material, they must be decontaminated in accordance to good laboratory practice. Wipe
the clean surface with a lint-free cloth, lightly soaked e. g. with the following disinfectants:
• Ethanol 70 %
• Microcide SQ 1:64
• Glutaraldehyde solution 4%
• Virkon solution 1-3%
Follow the instructions provided with the disinfectants.
It is not recommended to autoclave the EVOLVE Manual Pipettes. If autoclaving is
required, only the lower assembly of the EVOLVE single channel pipettes can be
autoclaved.
WARNING
Service is required after autoclaving the EVOLVE Manual Pipettes!
Do not autoclave the entire unit. Multichannel pipettes can NOT be
autoclaved, they might be damaged!
As-found calibration (measurement report, indicating “before” data) is not
possible after autoclaving!
5.3.1 Autoclaving the disassembled components
Place the disassembled components (see 5.2.1) into steam autoclave in an autoclave
pouch:
You may autoclave the components at 121°C, 1 bar overpressure for 20 minutes.
CAUTION
Service is required after autoclaving the EVOLVE Manual Pipettes!
Single channel 2–1000 µl: Single channel 5000 µl: